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Isolation, identification and capsule serotyping of Pasteurella multocida originated from swine

  

  1. (1. College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China; 2. Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, China)
  • Online:2016-04-25 Published:2016-04-27

Abstract: To investigate the serotypes of Pasteurella multocida in Sichuan, Chongqing and Yunnan, a total of 125 tissue samples were collected from intensive piggery during 2013 to 2014. PCR was performed to identify the isolated Pasteurella multocida and separate their capsular serotyping. It was shown that 11 strains of Pasteurella multocida were isolated from the lung tissues of swine. PCR typing assay indicated that 7 strains were serotype A (63.6%), 3 strains were serotype D (27.3%), 1 strain was serotype F (9.1%), the serotype B and serotype E were not found. It suggested that swine lung plague was mainly caused by serotype A Pasteurella multocida in these areas. In addition, a rare serotype F was isolated from the collected swine lung tissues. These results would provide scientific basis for the prevention and treatment of Pasteurella multocida in above areas.

Key words: swine, Pasteurella multocida, PCR, serotyping