Isolation， identification and biological characteristics analysis of Streptococcus parauberis

Isolation， identification and biological characteristics analysis of Streptococcus parauberis

（1. College of Veterinary Medicine， Sichuan Agricultural University， Chengdu 611130， China;2. Key Laboratory of Animal Disease and Human Health of Sichuan Province， Chengdu 611130， China）

Online:2016-05-25 Published:2016-05-19

Abstract

Abstract: One strain of Streptococcus parauberis was isolated from a large scale pig farm in Sichuan Province. In this study， gram staining， culture characteristics， biochemical tests， antibiotic sensitivity tests， and 16S rRNA gene sequence analysis were used to identify the strain characteristics and resistance to drugs. The results of the 16S rRNA sequencing of the isolated strain showed 99% homology with Streptococcus parauberis. The colonies of the isolated strain on TSA agar appeared to be smooth， circular and translucent and had βhemolysis on blood agar. The LD50 of isolated strain to mice was 206×107 cfu per mouse. The antibiotic sensitivity experiment showed that it was sensitive to cephalosporin antibiotics but insensitive to quinolone， tetracycline and aminoglycoside antibiotics. This study laid a foundation in clinical diagnosis， disease prevention and drug treatment for associate streptococcus disease.

Key words: Streptococcus parauberis, swine, 16S rRNA, isolation and identification

REN Meishen1，2， WANG Yin1，2，*， YANG Zexiao1， YAO Xueping1， WU Xulong1，2， XIAO Lu1，2. Isolation， identification and biological characteristics analysis of Streptococcus parauberis[J]. .

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Isolation， identification and biological characteristics analysis of Streptococcus parauberis

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