Abstract: In order to explore the impact of substrates on Pleurotus pulmonariuss yield and quality， different treatments of substrate preparation， sterilization and other conditions were set up. The results showed that the physicalchemical characters of substrates which were placed for more than 4 hours after bagging would deteriorate， and had a negative impact on mycelia and fructification growth. After sterilization under atmospheric pressure for 1， 2， 3， 4 and 5 times， the bulk density and electrical conductivity （EC） index of substrates increased， but the aeration porosity， moisture content and pH value decreased according to the increasing sterilization times. And the time of full bags of hyphae were 42， 49，56，63 and 70 d， respectively. The biological conversion were 52.26%， 45.85%， 45.19%， 40.10% and 40.91%， respectively， which indicated that the number of sterilization times had a negative effects on hyphae nutrition and reproductive growth. It can be concluded that substrates can maintain good physical and chemical properties and were beneficial for highquality and highyield of Pleurotus pulmonarius when they were sterilized under atmospheric pressure at 4 h after bagging， at 100 ℃ for 10 h.

Key words: Pleurotus pulmonarius, substrate, physicalchemical characters, correlation