Abstract: Lizaoxiang is a tea line bred in Lishui City， Zhejiang Province， whose genetic characteristics and diversity with other tea cultivars were the important basis for the examination of new varieties. The local major tea cultivars such as Jiaming No.1（Wuniuzao）， Yingshuang， Longjing 43 and Jiukeng were selected as experimental materials， and the total DNA of 6 materials including the above four cultivars and Lizaoxiang（mountain land type and field land type）were extracted using improved CTAB method and the genetic diversity and relationship of Lizaoxiang were analyzed using intersimple sequence repeat （ISSR） markers. The results indicated that Lizaoxiang is a stable tea line without variation in DNA profiles in different cultivation environment. Phylogenetic relationships revealed that Lizaoxiang was closest to Jiaming No.1 （Wuniuzao） but farthest from Longjing 43， while Yingshuang and Jiukeng were in between.

Key words: Lizaoxiang, ISSR, genetic diversity, genetic relationship