Isolation and identification of Xanthomonas axonopodis pv. citr on lemon samples from Taiwan

doi:10.3969/j.issn.1004-1524.2017.01.14

›› 2017, Vol. 29 ›› Issue (1): 101-105.DOI: 10.3969/j.issn.1004-1524.2017.01.14

• Plant Protection • Previous Articles Next Articles

Isolation and identification of Xanthomonas axonopodis pv. citr on lemon samples from Taiwan

CHEN Xianfeng, ZHANG Huili^*, ZHAO Lei

Ningbo Exit & Entry Inspection and Quarantine Bureau, Ningbo 315012, China

Received:2016-05-23 Online:2017-01-15 Published:2017-02-23

Abstract

Abstract: A typical symptom of citrus bacterial canker was observed on inspection of lemon samples imported from Taiwan. A strain 6503 was isolated from this sample and was identified by morphological observation, pathogenicity determination, specific-PCR detection and gyrB sequence analysis. The colony morphology of strain 6503 on nutrient agar medium (NA) was yellow, circular, entire, glistening, convex, mucoid. The pathogenicity test demonstrated that strain 6503 was pathogenic to the leaf of citrus. Strain 6503 can be amplified and produced 581 bp target band with specific primer Xac01/Xac02. Strain 6503 and X. axonopodis pv. citri were within the same clade in phylogenetic tree based on gyrB sequences. Based on these results above, strain 6503 was identified as X. axonopodis pv. citri.

Key words: lemon from Taiwan, X. axonopodis pv. citr, gyrB, pathogenicity, identification

CLC Number:

S436.661

CHEN Xianfeng, ZHANG Huili, ZHAO Lei. Isolation and identification of Xanthomonas axonopodis pv. citr on lemon samples from Taiwan[J]. , 2017, 29(1): 101-105.

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Isolation and identification of Xanthomonas axonopodis pv. citr on lemon samples from Taiwan

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