Effects of inoculation method of rhizobia on dry matter accumulation and yield of multiple soybean

doi:10.3969/j.issn.1004-1524.2021.10.03

Acta Agriculturae Zhejiangensis ›› 2021, Vol. 33 ›› Issue (10): 1808-1816.DOI: 10.3969/j.issn.1004-1524.2021.10.03

• Crop Science • Previous Articles Next Articles

Effects of inoculation method of rhizobia on dry matter accumulation and yield of multiple soybean

XU Yue(), XU Yaxin, HUANG Xingjun, WU Shu, CHEN Guodong^*(), WU Quanzhong, ZHAI Yunlong

College of Plant Sciences, Tarim University, Alar 843300, China

Received:2020-12-01 Online:2021-10-25 Published:2021-11-02
Contact: CHEN Guodong

Abstract

Abstract:

To explore the effects of different modes of rhizobium inoculation on the biomass accumulation and the yield, three isolated, identified and purified rhizobium strains, and multiple soybean varieties of SUINONG35 was selected as materials, the effects of the three rhizobium inoculation application methods of seed dressing, seed fertilizer, and drip application on the rhizobia nodule number, dry weight of root nodules, aboveground dry matter accumulation and distribution, yield and its composition were compared. The results showed that the rhizobium inoculation could promote the nodule formation of soybean roots, among which, seed dressing inoculation method of SN7-2, seed fertilization inoculation method for SMH12 and SN7-2, and drip inoculation for T6 had the best nodule effect. Seed dressing inoculation method of SN7-2, seed fertilization inoculation method for SMH12 and SN7-2, and drip inoculation for T6 coiled significantly promote the dry matter accumulation of soybean. Seed dressing method and seed fertilizer inoculation method of SN7-2, and drip inoculation method for T6 could promote dry matter distribution to reproductive organs. The seed yield of soybean improved by increasing the number of main stem nodes, pod number per plant, grain number per plant and 100 grain weight with different inoculation methods. The seed dressing method and seed fertilizer method were suitable for the SN7-2, whilst, for the T6, the suitable application method was dripping.

Key words: rhizobia, inoculation method, multiple soybean, dry matter accumulation, yield

CLC Number:

S565.1

XU Yue, XU Yaxin, HUANG Xingjun, WU Shu, CHEN Guodong, WU Quanzhong, ZHAI Yunlong. Effects of inoculation method of rhizobia on dry matter accumulation and yield of multiple soybean[J]. Acta Agriculturae Zhejiangensis, 2021, 33(10): 1808-1816.

Figures/Tables 5

References 29

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接种方式 Inoculation method	根瘤菌 Rhizobia	四节期 V4	盛花期 R2	盛荚期 R4	鼓粒期 R6
拌种Seed dressing	SMH12	0.006±0.001 cC	0.016±0.002 dD	0.191±0.001 cC	0.443±0.016 cC
	T6	0.033±0.000 aA	0.037±0.000 cC	0.230±0.006 aA	0.545±0.027 bB
	SN7-2	0.023±0.003 bB	0.099±0.008 aA	0.216±0.006 bB	1.610±0.008 aA
	CK	0.021±0.000 bB	0.069±0.006 bB	0.183±0.005 dC	0.390±0.056 cC
种肥Seed manure	SMH12	0.045±0.001 aA	0.034±0.003 cC	0.187±0.004 bB	0.601±0.011 bA
	T6	0.026±0.004 bB	0.076±0.002 bB	0.079±0.019 cC	0.278±0.032 cB
	SN7-2	0.040±0.003 aA	0.097±0.007 aA	0.266±0.009 aA	0.657±0.005 aA
	CK	0.024±0.001 bB	0.072±0.013 bB	0.186±0.006 bB	0.296±0.029 cB
滴施Drip irrigation	SMH12	0.016±0.002 cC	0.138±0.006 abA	0.209±0.004 cC	0.372±0.026 cC
	T6	0.055±0.002 aA	0.123±0.012 bAB	0.311±0.015 aA	0.753±0.019 aA
	SN7-2	0.023±0.001 bB	0.161±0.015 aA	0.261±0.017 bB	0.567±0.020 bB
	CK	0.022±0.001 bB	0.072±0.024 cB	0.189±0.006 dC	0.358±0.029 cC

接种方式 Inoculation method	根瘤菌 Rhizobia	四节期 V4	盛花期 R2	盛荚期 R4	鼓粒期 R6
拌种Seed dressing	SMH12	0.006±0.001 cC	0.016±0.002 dD	0.191±0.001 cC	0.443±0.016 cC
	T6	0.033±0.000 aA	0.037±0.000 cC	0.230±0.006 aA	0.545±0.027 bB
	SN7-2	0.023±0.003 bB	0.099±0.008 aA	0.216±0.006 bB	1.610±0.008 aA
	CK	0.021±0.000 bB	0.069±0.006 bB	0.183±0.005 dC	0.390±0.056 cC
种肥Seed manure	SMH12	0.045±0.001 aA	0.034±0.003 cC	0.187±0.004 bB	0.601±0.011 bA
	T6	0.026±0.004 bB	0.076±0.002 bB	0.079±0.019 cC	0.278±0.032 cB
	SN7-2	0.040±0.003 aA	0.097±0.007 aA	0.266±0.009 aA	0.657±0.005 aA
	CK	0.024±0.001 bB	0.072±0.013 bB	0.186±0.006 bB	0.296±0.029 cB
滴施Drip irrigation	SMH12	0.016±0.002 cC	0.138±0.006 abA	0.209±0.004 cC	0.372±0.026 cC
	T6	0.055±0.002 aA	0.123±0.012 bAB	0.311±0.015 aA	0.753±0.019 aA
	SN7-2	0.023±0.001 bB	0.161±0.015 aA	0.261±0.017 bB	0.567±0.020 bB
	CK	0.022±0.001 bB	0.072±0.024 cB	0.189±0.006 dC	0.358±0.029 cC

接种方式 Inoculation method	根瘤菌 Rhizobia	茎 Straw
接种方式 Inoculation method	根瘤菌 Rhizobia	V4	R2	R4	R6	R8	V4	R2	R4	R6	R4	R6	R8
拌种	SMH12	25.60	22.07	25.29	11.33	10.00	74.40	77.93	45.14	30.67	29.57	58.00	90.00
Seed dressing	T6	24.78	21.15	22.76	12.70	9.13	75.22	78.85	52.59	30.85	24.66	56.45	90.87
	SN7-2	25.90	21.92	23.52	12.65	11.19	74.10	78.08	55.16	30.33	21.32	57.02	88.81
	CK	27.61	24.27	26.15	13.17	11.29	72.39	75.73	52.91	30.92	20.94	55.91	88.71
种肥	SMH12	23.49	25.94	22.95	16.46	8.56	76.51	74.06	55.76	38.13	21.29	45.41	91.44
Seed manure	T6	20.46	26.12	21.16	13.47	8.17	79.54	73.88	57.07	34.91	21.77	51.62	91.83
	SN7-2	24.47	25.13	21.35	15.33	8.55	75.53	74.87	52.78	35.74	25.87	48.92	91.45
	CK	24.55	27.67	25.32	15.41	10.23	75.45	72.33	53.80	35.97	20.89	48.62	89.77
滴施	SMH12	25.93	25.11	21.69	16.15	9.72	74.07	74.89	53.66	42.42	24.65	41.43	90.28
Drip	T6	24.59	26.45	26.01	14.02	9.58	75.41	73.55	53.98	38.55	20.01	47.43	90.42
irrigation	SN7-2	22.65	24.86	22.00	16.57	12.06	77.35	75.14	57.24	39.20	20.76	44.23	87.94
	CK	26.46	27.84	25.11	13.51	11.32	73.54	72.16	53.92	36.45	20.97	50.04	88.68

接种方式 Inoculation method	根瘤菌 Rhizobia	茎 Straw
接种方式 Inoculation method	根瘤菌 Rhizobia	V4	R2	R4	R6	R8	V4	R2	R4	R6	R4	R6	R8
拌种	SMH12	25.60	22.07	25.29	11.33	10.00	74.40	77.93	45.14	30.67	29.57	58.00	90.00
Seed dressing	T6	24.78	21.15	22.76	12.70	9.13	75.22	78.85	52.59	30.85	24.66	56.45	90.87
	SN7-2	25.90	21.92	23.52	12.65	11.19	74.10	78.08	55.16	30.33	21.32	57.02	88.81
	CK	27.61	24.27	26.15	13.17	11.29	72.39	75.73	52.91	30.92	20.94	55.91	88.71
种肥	SMH12	23.49	25.94	22.95	16.46	8.56	76.51	74.06	55.76	38.13	21.29	45.41	91.44
Seed manure	T6	20.46	26.12	21.16	13.47	8.17	79.54	73.88	57.07	34.91	21.77	51.62	91.83
	SN7-2	24.47	25.13	21.35	15.33	8.55	75.53	74.87	52.78	35.74	25.87	48.92	91.45
	CK	24.55	27.67	25.32	15.41	10.23	75.45	72.33	53.80	35.97	20.89	48.62	89.77
滴施	SMH12	25.93	25.11	21.69	16.15	9.72	74.07	74.89	53.66	42.42	24.65	41.43	90.28
Drip	T6	24.59	26.45	26.01	14.02	9.58	75.41	73.55	53.98	38.55	20.01	47.43	90.42
irrigation	SN7-2	22.65	24.86	22.00	16.57	12.06	77.35	75.14	57.24	39.20	20.76	44.23	87.94
	CK	26.46	27.84	25.11	13.51	11.32	73.54	72.16	53.92	36.45	20.97	50.04	88.68

接种方式 Inoculation method	根瘤菌 Rhizobia	主茎节数 Node number of main stem	单株荚数 Pod number per plant	单株粒数 Seed number per plant	百粒重 100-seed weight/g	产量 Yield/ (kg·hm^-2)
拌种	SMH12	12.8±1.9 aA	38.6±0.5 cC	106.4±0.7 bB	20.2±0.3 aA	4 651.5±64.0 cC
Seed dressing	T6	13.2±0.4 aA	35.4±0.5 bB	98.0±1.0 aA	18.8±0.3 cB	5 208.8±22.0 bB
	SN7-2	13.4±1.1 aA	44.6±0.5 cBC	105.0±0.9 abAB	19.6±0.5 abAB	5 946.0±52.4 aA
	CK	12.6±1.5 aA	33.4±0.9 aA	88.2±0.8 aAB	19.2±0.1 bcAB	4 120.6±18.0 dD
种肥	SMH12	11.4±0.9 aA	26.2±0.8 bB	77.0±1.0 bB	20.6±0.3 aA	5 446.6±58.7 aA
Seed manure	T6	11.0±0.7 aA	26.0±0.7 bB	67.0±0.7 aA	18.9±0.1 bB	3 305.3±88.4 cC
	SN7-2	11.4±0.9 aA	31.6±0.5 bB	82.6±0.9 bB	19.2±0.3 bB	5 461.6±61.7 aA
	CK	11.0±1.0 aA	25.8±0.8 aA	66.6±0.9 aA	19.1±0.1 bB	3 957.4±56.2 bB
滴施	SMH12	11.6±1.7 bA	37.4±0.9 bA	101.8±1.5 bB	16.5±0.2 cB	4 739.6±52.4 cB
Drip irrigation	T6	14.4±1.1 aA	48..0±0.7 aA	117.0±1.2 aA	17.4±0.0 bA	5 888.6±61.0 aA
	SN7-2	13.4±1.1 abA	38..0±1.2 bA	102.6±0.9 bB	17.9±0.3 aA	4 948.4±46.7 bB
	CK	11.8±0.8 bA	36.6±0.5 bA	84.0±1.2 cC	16.4±0.3 cB	4 094.8±54.7 dC

Effects of inoculation method of rhizobia on dry matter accumulation and yield of multiple soybean

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