Effects of different diets on growth performance and environmental water quality of Scylla paramamosain cultured in carb apartment

doi:10.3969/j.issn.1004-1524.2022.11.10

Acta Agriculturae Zhejiangensis ›› 2022, Vol. 34 ›› Issue (11): 2404-2415.DOI: 10.3969/j.issn.1004-1524.2022.11.10

• Animal Science • Previous Articles Next Articles

Effects of different diets on growth performance and environmental water quality of Scylla paramamosain cultured in carb apartment

CAI Yilong¹(), ZHANG Libing², HU Gaoyu¹, XIAO Guoqiang¹, CAI Jingbo¹, ZHANG Xiang^*()

1. Zhejiang Key Laboratory of Exploitation and Preservation of Coastal Bio-Resource, Wenzhou Key Laboratory of Marine Biological Genetics and Breeding, Zhejiang Mariculture Research Institute, Wenzhou 325005, Zhejiang, China
2. Yueqing Agricultural and Rural Bureau, Yueqing 325699, Zhejiang, China

Received:2022-03-02 Online:2022-11-25 Published:2022-11-29
Contact: ZHANG Xiang

Abstract

Abstract:

To study the effects of different baits on growth performance and feeding environment of Scylla paramamosain in crab apartment, the growth and environmental water quality parameters of S. paramamosain during aquaculture were analyzed by feeding different baits (Sinonovacula constricta, fresh fish meat, compound feed for green crab and eel compound feed). The results showed that the specific growth rate of S. paramamosain in S. constricta group was the highest (1.15%·d^-1), followed by the eel feed group (0.56%·d^-1). Besides the S. constricta group, the specific growth rate of other bait groups were significantly correlated with pH, dissolved oxygen and ammonia nitrogen, etc. In terms of survival rate, there was no significant correlation between S. constricta group and water quality indexes, fish group was only significantly correlated with phosphate and nitrite, and significant correlation between two artificial compound diets and active phosphate, suspended particulate matter, dissolved oxygen, pH, nitrite and other indexes. In addition to water temperature and salinity, water quality was mainly affected by feeding. The comprehensive pollution index of water quality showed that the four bait groups were in the state of pollution and environmental eutrophication (NQI≥8.5), but the eutrophication degree of S. constricta group was the lowest. The results showed that S. constricta was the best bait for crab apartment breeding, and the green crab fed by S. constricta had higher growth performance and better breeding water environment. Considering the breeding cost, compound feed was also suitable for green crab breeding in crab apartment, but the water quality of feeding environment needed to be further improved. The result provided a technical basis for the selection of bait and the optimization of management measures in the facility culture of S. paramamosain.

Key words: crab apartment, Scylla paramamosain, growth performance, feeding environment, water quality indicator

CLC Number:

S963
S966.5

CAI Yilong, ZHANG Libing, HU Gaoyu, XIAO Guoqiang, CAI Jingbo, ZHANG Xiang. Effects of different diets on growth performance and environmental water quality of Scylla paramamosain cultured in carb apartment[J]. Acta Agriculturae Zhejiangensis, 2022, 34(11): 2404-2415.

Figures/Tables 11

References 39

[1]	杨正宽, 方怀义, 雷宁, 等. 拟穴青蟹养殖技术要点及养殖模式优劣分析[J]. 水产养殖, 2019, 40(3): 19-21.
	YANG Z K, FANG H Y, LEI N, et al. Analysis on the key points of culture technology and the advantages and disadvantages of breeding mode in Scylla paramamosain[J]. Journal of Aquaculture, 2019, 40(3): 19-21. (in Chinese)
[2]	施兆鸿, 夏连军, 王建钢. 影响锯缘青蟹幼体成活的几个因素[J]. 海洋渔业, 2004, 26(4): 281-285.
	SHI Z H, XIA L J, WANG J G. Factors affecting survival rate of mud crab Scylla serrata larvae[J]. Marine Tieheries, 2004, 26(4): 281-285. (in Chinese with English abstract)
[3]	农业部渔业局. 中国渔业统计年鉴2020[M]. 北京: 中国农业出版社, 2020.
[4]	农业部渔业渔政管理局. 中国渔业统计年鉴-2016[M]. 北京: 中国农业出版社, 2016.
[5]	农业部渔业渔政管理局. 中国渔业统计年鉴-2017[M]. 北京: 中国农业出版社, 2017.
[6]	农业部渔业局. 中国渔业统计年鉴2018[M]. 北京: 中国农业出版社, 2018.
[7]	农业部渔业局. 中国渔业统计年鉴2019[M]. 北京: 中国农业出版社, 2019.
[8]	艾春香, 刘建国, 林琼武, 等. 青蟹的营养需求研究及其配合饲料研制[J]. 水产学报, 2007, 31(S1): 122-128.
	AI C X, LIU J G, LIN Q W, et al. Nutrient requirements and development of formulated diets of mud crab, Scylla sp[J]. Journal of Fisheries of China, 2007, 31(S1): 122-128. (in Chinese with English abstract)
[9]	黄莹, 何亮华, 黄贞胜, 等. 青蟹人工配合饲料的营养需求初探[J]. 当代水产, 2018, 43(9): 96-97.
	HUANG Y, HE L H, HUANG Z S, et al. Preliminary study on nutritional requirements of artificial compound feed of mud crab, Scylla sp[J]. Current Fisheries, 2018, 43(9): 96-97. (in Chinese)
[10]	王贤丰, 赵艳飞, 宋志飞, 等. 应用高通量测序技术分析拟穴青蟹肠道及其养殖环境菌群结构[J]. 中国水产科学, 2017, 24(6): 1245-1253.
	WANG X F, ZHAO Y F, SONG Z F, et al. Application of high-throughput sequencing techniques for analyzing bacterial communities in pond-raised mud crab (Scylla paramamosain) intestine and its aquaculture environment[J]. Journal of Fishery Sciences of China, 2017, 24(6): 1245-1253. (in Chinese with English abstract) DOI URL
[11]	许明珠, 张琴, 董兰芳, 等. 不同糖源对拟穴青蟹仔蟹的生长、体成分及消化酶的影响[J]. 水产科学, 2020, 39(2): 175-181.
	XU M Z, ZHANG Q, DONG L F, et al. Effects of different dietary carbohydrate sources on growth performance, body composition and digestive enzyme activities of juvenile green crab Scylla paramamosain[J]. Fisheries Science, 2020, 39(2): 175-181. (in Chinese with English abstract)
[12]	杨坤, 胡星明, 卢文轩. 三角帆蚌对综合养殖水体水质及鱼蚌生长的影响[J]. 淡水渔业, 2020, 50(1): 82-86.
	YANG K, HU X M, LU W X. Effects of Hyriopsis cumingii on water quality and growth of other mussel and fish in the integrated culture[J]. Freshwater Fisheries, 2020, 50(1): 82-86. (in Chinese with English abstract)
[13]	曾媛媛. 环境因子对拟穴青蟹生理生化影响[D]. 厦门: 厦门大学, 2009.
	ZENG Y Y. Effects of the environmental factors on physiology-biochemistry of Scylla paramamosain[D]. Xiamen: Xiamen University, 2009. (in Chinese with English abstract)
[14]	黄海涛. 温度、盐度、溶解氧、氨氮、亚硝酸盐氮对拟穴青蟹蜕壳的影响[D]. 湛江: 广东海洋大学, 2011.
	HUANG H T. Effects of temperature, salinity, dissolved oxygen, ammonia-N and nitrite-N on the molting of Scylla paramamosain[D]. Zhanjiang: Guangdong Ocean University, 2011. (in Chinese with English abstract)
[15]	张贵. 溶解氧、盐度、氨氮、亚硝酸盐氮对三疣梭子蟹存活和摄饵的影响[D]. 湛江: 广东海洋大学, 2012.
	ZHANG G. Effect of dissolved oxygen, salinity, ammonia-N and nitrite-N on the survivoal and food intake of Portunus trituberculatus[D]. Zhanjiang: Guangdong Ocean University, 2012. (in Chinese with English abstract)
[16]	王煜恒, 李庆, 陈军, 等. 不同溶氧浓度下非离子氨和亚硝酸盐对中华绒螯蟹的急性毒性[J]. 水产学杂志, 2019, 32(4): 38-44.
	WANG Y H, LI Q, CHEN J, et al. Acute toxicity of nonionic ammonia and nitrite to Chinese mitten handed crab Eriocheir sinenisis at different levels of dissolved oxygen[J]. Chinese Journal of Fisheries, 2019, 32(4): 38-44. (in Chinese with English abstract)
[17]	孙晓飞, 花勃. 室内工厂化循环水立体养殖锯缘青蟹技术[J]. 中国水产, 2017(5): 80-82.
	SUN X F, HUA B. Cultivation of Scylla serrata in indoor three-dimensional industrialized circulating water[J]. China Fisheries, 2017(5): 80-82. (in Chinese)
[18]	曾尚伟, 吴喜标, 裴琨. 红树林滩涂瓦缸养殖锯缘青蟹关键技术[J]. 中国水产, 2011(1): 43-44.
	ZENG S W, WU X B, PEI K. Key techniques for culturing Scylla serrata in vats on mangrove Beach[J]. China Fisheries, 2011(1): 43-44. (in Chinese)
[19]	王桂忠, 李少菁, 陈志刚. 青蟹(Scylla spp.)养殖现状及拟穴青蟹(S. paramamosain)种群生物学研究[J]. 厦门大学学报(自然科学版), 2016, 55(5): 617-623.
	WANG G Z, LI S J, CHEN Z G. Status of mud crabs(Scylla spp.) farming and studies on the population biology of S. paramamosain[J]. Journal of Xiamen University (Natural Science), 2016, 55(5): 617-623. (in Chinese with English abstract)
[20]	聂晓锋, 李春晓. “蟹公寓”养殖青蟹技术[J]. 海洋与渔业, 2018(2): 56-57.
	NIE X F, LI C X. Technique of culturing green crab in “crab apartment”[J]. Ocean & Fishery, 2018(2): 56-57. (in Chinese)
[21]	罗蔚华, 钱晓薇, 杨曙光, 等. 乐清产缢蛏(Sinonovacula constricta)肉营养成分的研究[J]. 江西科学, 2006, 24(5): 360-362.
	LUO W H, QIAN X W, YANG S G, et al. Study on the nutritive content of Sinonovacula constricta[J]. Jiangxi Science, 2006, 24(5): 360-362. (in Chinese with English abstract)
[22]	李太武, 林叶, 苏秀榕. 不同群体缢蛏营养成分的多元性分析[J]. 食品科学, 2008, 29(11): 548-551.
	LI T W, LIN Y, SU X R. Multivariation analysis of nutrients of sinonovaculla Constricta lamarck in different populations[J]. Food Science, 2008, 29(11): 548-551. (in Chinese with English abstract)
[23]	贾成霞, 曲疆奇, 李永刚, 等. 密云水库鲢鱼、鳙鱼营养成分分析与评价[J]. 水产科学, 2019, 38(1): 40-47.
	JIA C X, QU J Q, LI Y G, et al. Nutritional compositions in muscles of silver carp Hypophthalmichthys molitrix and bighead carp aristichthys nobilis in Miyun reservoir[J]. Fisheries Science, 2019, 38(1): 40-47. (in Chinese with English abstract)
[24]	杨丽丽, 杨筱珍, 赵柳兰, 等. 冰鲜野杂鱼和配合饲料对中华绒螯蟹幼蟹生长、消化酶活力及血细胞的影响研究[J]. 复旦学报(自然科学版), 2011, 50(5): 619-624.
	YANG L L, YANG X Z, ZHAO L L, et al. Effects of two different diets on the growth, digestive enzyme activity and haemocytes in juvenile Chinese mitten crab(Eriocheir sinensis)[J]. Journal of Fudan University (Natural Science), 2011, 50(5): 619-624. (in Chinese with English abstract)
[25]	陈立侨, 堵南山, 赖伟. 水体和配合饲料中钙、磷含量对河蟹生长的影响[J]. 淡水渔业, 1994, 24(1): 13-15.
	CHEN L Q, DU N S, LAI W. Effects of calcium and phosphorus contents in water and compound feed on growth of river crab[J]. Freshwater Fisheries, 1994, 24(1): 13-15. (in Chinese)
[26]	黄伟卿, 陈爱平, 张艺, 等. 软颗粒饲料对“红膏蟹”培育生长、存活和营养成分的影响[J]. 水产科学, 2018, 37(5): 605-611.
	HUANG W Q, CHEN A P, ZHANG Y, et al. Effect of soft pelleted feed on growth, survival and ovarian composition in mature mud crab[J]. Fisheries Science, 2018, 37(5): 605-611. (in Chinese with English abstract)
[27]	周凡, 陈丽芝, 王鼎南, 等. 配合饲料在拟穴青蟹-脊尾白虾混养模式中的应用效果初探[J]. 水产科技情报, 2017, 44(5): 248-251.
	ZHOU F, CHEN L Z, WANG D N, et al. Preliminary study on the application effect of compound feed in polyculture mode of mud crab (Scylla paramichthys) and white shrimp (Mystus carinatus)[J]. Fisheries Science & Technology Information, 2017, 44(5): 248-251. (in Chinese)
[28]	丁小丰, 杨玉娇, 金珊, 等. 温度变化对锯缘青蟹免疫因子的胁迫影响[J]. 水产科学, 2010, 29(1): 1-6.
	DING X F, YANG Y J, JIN S, et al. The stress effects of temperature fluctuation on immune factors in crab Scylla serrata[J]. Fisheries Science, 2010, 29(1): 1-6. (in Chinese with English abstract)
[29]	TOMASETTI S J, MORRELL B K, MERLO L R, et al. Individual and combined effects of low dissolved oxygen and low pH on survival of early stage larval blue crabs, Callinectes sapidus[J]. PLoS One, 2018, 13(12): e0208629. DOI URL
[30]	王桂忠, 林淑君, 林琼武, 等. 盐度对锯缘青蟹幼体存活与生长发育的影响[J]. 水产学报, 1998, 22(1): 89-92.
	WANG G Z, LIN S J, LIN Q W, et al. Effects of salinity on survival and development of the mud crab Scylla serrata[J]. Journal of Fisheries of China, 1998, 22(1): 89-92. (in Chinese)
[31]	袁春营, 崔青曼. 盐度影响水产养殖动物生长发育的研究进展[J]. 水产科学, 2004, 23(5): 41-42.
	YUAN C Y, CUI Q M. A review: influence of salinity on development and growth of aquatic animals in aquaculture[J]. Fisheries Science, 2004, 23(5): 41-42. (in Chinese with English abstract)
[32]	吴琴瑟. 虾蟹养殖高产技术: 高效益养殖技术丛书[M]. 2版. 北京: 中国农业出版社, 1998.
[33]	刘璐, 杨玉娇, 王国良. pH突变对拟穴青蟹免疫因子的胁迫影响[J]. 宁波大学学报(理工版), 2009, 22(4): 484-489.
	LIU L, YANG Y J, WANG G L. Effect of sudden changes in pH on the immune factors of Scylla paramamosain[J]. Journal of Ningbo University (Natural Science & Engineering Edition), 2009, 22(4): 484-489. (in Chinese with English abstract)
[34]	魏虎进, 程飞, 朱小明, 等. 以缢蛏为主的生态综合养殖池塘氮磷流转研究[J]. 厦门大学学报(自然科学版), 2013, 52(2): 254-258.
	WEI H J, CHENG F, ZHU X M, et al. The budgets of nitrogen and phosphorus in the integrated multi-trophic aquaculture(IMTA) ponds[J]. Journal of Xiamen University (Natural Science), 2013, 52(2): 254-258. (in Chinese with English abstract)
[35]	金珊, 程岩雄, 杨玉姣, 等. 铵氮胁迫对拟穴青蟹Scylla paramamosain免疫力的影响[J]. 浙江农业学报, 2008, 20(6): 402-407.
	JIN S, CHENG Y X, YANG Y J, et al. Effects of ammonium nitrogen stress on the immunity of Scylla paramamosain[J]. Acta Agriculturae Zhejiangensis, 2008, 20(6): 402-407. (in Chinese with English abstract)
[36]	于忠利, 乔振国, 王建钢. 两种环境因子对青蟹蜕壳影响的研究[J]. 现代渔业信息, 2009, 24(11): 25-27.
	YU Z L, QIAO Z G, WANG J G. Study on effects of two environmental factors on molting of Scylla spp[J]. Modern Fisheries Information, 2009, 24(11): 25-27. (in Chinese)
[37]	孙国铭, 汤建华, 仲霞铭. 氨氮和亚硝酸氮对南美白对虾的毒性研究[J]. 水产养殖, 2002, 23(1): 22-24.
	SUN G M, TANG J H, ZHONG X M. Toxicity research of ammonia nitrogen and nitrite nitrogen to Penaeus vannamei[J]. Journal of Aquaculture, 2002, 23(1): 22-24. (in Chinese with English abstract)
[38]	蔡继晗, 沈奇宇, 郑向勇, 等. 氨氮污染对水产养殖的危害及处理技术研究进展[J]. 浙江海洋学院学报(自然科学版), 2010, 29(2): 167-172, 195.
	CAI J H, SHEN Q Y, ZHENG X Y, et al. Advancement in researches of ammonia pollution hazards on aquaculture and its treatment technology[J]. Journal of Zhejiang Ocean University (Natural Science), 2010, 29(2): 167-172, 195. (in Chinese with English abstract)
[39]	刘桂芳. 水产养殖中氨氮和亚硝酸盐氮的危害及防治[J]. 养殖与饲料, 2018(5): 37-38.
	LIU G F. Harm and prevention of ammonia nitrogen and nitrite nitrogen in aquaculture[J]. Animals Breeding and Feed, 2018(5): 37-38. (in Chinese)

基本营养成分 Proximate composition	水分 Moisture	粗蛋白质 Crude protein	粗灰分 Crude ash	粗脂肪 Crude lipid
鳗鱼饲料	9.80	44.10	16.66	3.92
Eel feed
青蟹配合饲料	10.40	41.68	1.30	10.70
Compound feed
缢蛏^[21-22]	85.56	10.16	2.82	0.55
Sinonovacula constricta
鱼肉^[23]	79.10	18.40	1.17	0.60
Fresh fish

基本营养成分 Proximate composition	水分 Moisture	粗蛋白质 Crude protein	粗灰分 Crude ash	粗脂肪 Crude lipid
鳗鱼饲料	9.80	44.10	16.66	3.92
Eel feed
青蟹配合饲料	10.40	41.68	1.30	10.70
Compound feed
缢蛏^[21-22]	85.56	10.16	2.82	0.55
Sinonovacula constricta
鱼肉^[23]	79.10	18.40	1.17	0.60
Fresh fish

组别 Groups	特定生长率 Special growth rate/(%·d^-1)	肝胰腺指数 Hepatopancreas index/%	存活率 Survival rate/%
缢蛏组SC	1.15±0.12 a	6.97±2.48 a	48.33±0.03 a
鱼肉组FF	0.49±0.04 b	4.96±1.20 a	55.00±0.05 a
配合饲料组CF	0.51±0.08 b	7.28±1.01 a	48.33±0.03 a
鳗鱼饲料组EF	0.56±0.05 b	6.82±1.26 a	43.33±0.03 a

组别 Groups	特定生长率 Special growth rate/(%·d^-1)	肝胰腺指数 Hepatopancreas index/%	存活率 Survival rate/%
缢蛏组SC	1.15±0.12 a	6.97±2.48 a	48.33±0.03 a
鱼肉组FF	0.49±0.04 b	4.96±1.20 a	55.00±0.05 a
配合饲料组CF	0.51±0.08 b	7.28±1.01 a	48.33±0.03 a
鳗鱼饲料组EF	0.56±0.05 b	6.82±1.26 a	43.33±0.03 a

组别 Groups	pH	溶解氧 Dissolved oxygen/ (mg·L^-1)	化学需氧量 COD/ (mg·L^-1)	悬浮颗粒物 Suspended particulate matter/ (mg·L^-1)	活性磷酸盐 Phosphate/ (mg·L^-1)	氨氮 Ammonium/ (mg·L^-1)	无机氮 inorganic nitrogen/ (mg·L^-1)
对照组CK	7.7±0.3 a	7.1±0.9 a	2.9±2.4 b	59.9±26.0 b	0.024±0.027 c	0.161±0.204 b	0.150±0.236 b
缢蛏组SC	7.4±0.1 b	4.3±0.7 b	2.9±1.4 b	57.3±10.2 b	0.168±0.050 b	0.851±0.617 ab	1.165±0.043 a
鱼肉组FF	7.5±0.2 b	4.8±1.6 b	3.0±0.7 b	53.7±6.7 b	0.440±0.034 a	0.767±0.453 ab	1.264±0.114 a
配合饲料组CF	7.4±0.4 bc	4.4±1.8 b	4.7±1.2 a	62.6±10.8 a	0.220±0.014 b	0.563±0.338 b	0.804±0.091 a
鳗鱼组EF	7.2±0.2 c	3.0±1.2 c	5.0±1.8 a	72.6±22.1 a	0.132±0.044 b	1.018±0.447 a	1.452±0.121 a

Effects of different diets on growth performance and environmental water quality of Scylla paramamosain cultured in carb apartment

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组别 Groups	活性磷酸盐 Phosphate	氨氮 Ammonium	硝态氮 Nitrate	亚硝氮 Nitrite	悬浮颗粒物 Suspended particulate matter	化学需氧量 COD	溶解氧 Dissolved oxygen	pH
缢蛏组SC	-0.434	-0.580	0.605	0.243	-0.075	-0.321	0.080	-0.327
鱼肉组FF	0.502	0.720^*	0.363	0.287	0.618	0.610	0.603	-0.527
配合饲料组CF	-0.585	-0.287	0.260	-0.244	0.147	-0.026	-0.032	-0.731^*
鳗鱼饲料组EF	-0.394	-0.305	0.182	0.535	-0.192	-0.443	0.670^*	-0.199

各组存活率 SR	活性磷酸盐 Phosphate	氨氮 Ammonium	硝态氮 Nitrate	亚硝氮 Nitrite	悬浮颗粒物 Suspended particulate matter	化学需氧量 COD	溶解氧 Dissolved oxygen	pH
缢蛏组SC	0.093	-0.035	0.502	0.569	0.257	0.327	0.129	0.296
鱼肉组FF	0.976^*	0.241	0.356	0.803^*	0.111	-0.398	0.674	0.486
配合饲料组CF	0.747^*	0.043	0.285	0.605	-0.825^*	-0.127	0.818^*	0.775^*
鳗鱼饲料组EF	0.817^*	0.029	0.547	0.844^*	0.266	0.628	0.670	0.181

组别 Groups	养殖过程中平均NQI值 Average NQI value in the process of culture	评价 Evaluation
缢蛏组SC	8.50	富营养化 Eutrophication
鱼肉组FF	17.77	富营养化 Eutrophication
配合饲料CF	10.25	富营养化 Eutrophication
鳗鱼饲料EF	8.57	富营养化 Eutrophication
对照组水样CS	2.61	中营养水平 Mesotrophic level

组别 Groups	养殖成本/元 Production cost/Yuan	青蟹增重 Green crabs gain weight/g	青蟹售价/(元·g^-1) Green crab price/(Yuan·g^-1)	收入/元 Revenue/Yuan
缢蛏组SC	194.88	1 147.49	0.2	229.50
鱼肉组FF	66.53	498.63	0.2	99.73
配合饲料组CF	24.36	349.30	0.2	69.86
鳗鱼饲料组EF	21.84	379.24	0.2	75.85

[1]	AN Yawen, YANG Xiaodong, GAO Zhixiong, GUO Shaoqian, GAO Aiwu, YANG Jinli, WANG Hairong. Effects of adding Sophora alopecuroides to high grain diet on growth and serum biochemical indexes of lambs [J]. Acta Agriculturae Zhejiangensis, 2022, 34(5): 908-914.
[2]	WU Tao, WEI Yuming, JIANG Xiaofan, HUANG Jie, YANG Farong, CHEN Guoshun, CAI Yuan, JIAO Ting, ZHAO Shenguo. Effects of Chenopodium quinoa in diets on growth performance, slaughter performance, organ index and intestinal morphology of Luhua chickens [J]. Acta Agriculturae Zhejiangensis, 2022, 34(2): 255-265.
[3]	JIANG Xiaofan, WU Tao, WEI Yuming, YANG Farong, CHEN Guoshun, JIAO Ting, CAI Yuan, ZHAO Shengguo. Effects of dietary oregano essential oil on growth performance, slaughter performance, organ indexes and intestinal morphology of Luhua chickens [J]. Acta Agriculturae Zhejiangensis, 2022, 34(1): 41-49.
[4]	LIU Hong\\|lu， FAN Lei， DAI Xi\\|xi， ZHANG Fei\\|yan， PAN Kang\\|cheng*. Effects of Saccharomyces cerevisiae selenium and Clostridium butyricum additive on growth， antioxidant ability and intestinal microflora#br# [J]. , 2015, 27(9): 1529-.
[5]	ZHOU Fan1， WANG Ya\\|qin2， LIN Ling3， MA Wen\\|jun1， DING Xue\\|yan1，*， HE Feng1， XUE Hui\\|li1. Effects of dietary protein levels on growth performance and digestive enzyme activities of Chinese soft\\|shelled turtle Pelodiscus sinensis juvenile [J]. , 2014, 26(6): 1442-.
[6]	CHEN Shao\\|meng;LU Fu\\|zeng;*;HUA Wei\\|dong;XYU Ru\\|hai;CHU Xiao\\|hong;DAI Li\\|he;HU Jin\\|ping;SONG Wei\\|long. Effect of spraying Chinese herbal medicine in the pigpen on the diarrhea occurrence of early weanling piglets [J]. , 2013, 25(6): 0-1227.
[7]	ZHANG Yong-jing;XU Wei-na;LIU Wen-bin*;LI Gui-feng;XIA Wei;SHAO Xian-ping. Effects of dietary Pb on growth performance and enzyme activities of Jian carp [J]. , 2012, 24(2): 0-227.
[8]	WEN Jing;SUN Jian-an;ZHOU Xu-xia;LI Wei-fen;*. Effects of Enterococcus faecium on growth performance, immune and antioxidant function of piglets [J]. , 2011, 23(1): 70-73.
[9]	ZHANG Jian-zhong;LU Jian-jun;*. Effects of soybean antigen protein on the growth performance of weaning piglets [J]. , 2010, 22(6): 843-847.
[10]	XU Wen;DONG Qi-guo;WANG Gai-qin;LUO Lan;WANG Tian;*. Effects of Bioplex Cu on sow productivity, piglet growth performance and serum mineral contents [J]. , 2010, 22(4): 0-473.
[11]	CAI Zhao-wei;LIU Xiao-feng;HUA Xu-chuan;HUA Wei-dong;ZHANG Jin-zhi;*. Effect of different levels of compound gastrointestinal microbial modulator on the growth performance and small intestinal structure of weaned pigs [J]. , 2010, 22(4): 0-478.