Dynamic profile of genes related to seed dormancy under high humidity condition during late stage of rice grain filling

doi:10.3969/j.issn.1004-1524.2022.06.01

Acta Agriculturae Zhejiangensis ›› 2022, Vol. 34 ›› Issue (6): 1103-1113.DOI: 10.3969/j.issn.1004-1524.2022.06.01

• Crop Science • Previous Articles Next Articles

Dynamic profile of genes related to seed dormancy under high humidity condition during late stage of rice grain filling

DONG Yuanyuan¹^,²(), XU Heng², ZHANG Hua², ZHANG Heng², WANG Fulin², GU Nana², ZHU Ying²^,^*()

1. College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua 321004, Zhejiang, China
2. State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Received:2022-02-22 Online:2022-06-25 Published:2022-06-30
Contact: ZHU Ying

Abstract

Abstract:

To investigate the effect of high humidity on pre-harvest sprouting in the late stage of rice grain filling, milky stage rice plants, at 26, 28, 30, 32 DAP (day after pollination) respectively, were undergo high humidity treatment in the artificial climate chamber (28 ℃/22 ℃, 12 h light/12 h dark cycle, relative humidity were 60%, photosynthetic photon flux density were 600 μmol·m^-2·s^-1). Spikelet was sprayed periodically by water (spray 500 mL water once every 3 h per bots, 5 d in total) to mimic continuous rainy conditions, and the viviparity of mature seeds were then investigated. Then, developing seeds at DAP30 were undergo same treatment and the dynamic expression of ABA and GA synthesis and metabolism and α-starch hydrolase and other related genes were investigated at different time under high humidity treatment. The results indicated that seed maturity was an important factor affecting viviparity induced by high humidity. Few seeds were sprouted at DAP26 and DAP28 but most of seeds could be sprouted after DAP30 under high humidity treatments. The results showed that high humidity treatment could rapidly induce the expression of abscisic acid (ABA) degradation related genes, such as OsABA8ox1 and OsABA8ox3, and gibberellin (GA) synthesis genes such as OsGA3ox2 and OsGA20ox1, and simultaneously inhibited the expression of key ABA synthesis genes OsNECD1, OsNECD2 and OsNECD3. Many α-starch hydrolase genes, such as OsAmy1A, OsAmy3B, OsAmy3C and OsAmy3D were activated after 72 h water-spraying treatment and provided material and energy for seed germination. The results of this study would help us to understand the molecular mechanism of pre-harvest sprouting under rainy climate.

Key words: high humidity, pre-harvest spouting, rice, gene expression

CLC Number:

S511

DONG Yuanyuan, XU Heng, ZHANG Hua, ZHANG Heng, WANG Fulin, GU Nana, ZHU Ying. Dynamic profile of genes related to seed dormancy under high humidity condition during late stage of rice grain filling[J]. Acta Agriculturae Zhejiangensis, 2022, 34(6): 1103-1113.

Figures/Tables 7

References 24

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基因 Gene	上游引物 Forward primer (5'→3')	下游引物 Reverse primer (5'→3')	产物长度 Product length/bp
OsNCED1	AGCCTCGGTCTTCCAATTTT	CACCCAACACAAAAGCTACG	125
OsNCED2	TCATTCCAAAACACCTTCCA	TCCGGGGACCTCCTATGTAT	113
OsNCED3	ACGTGATCAAGAAGCCGTACCT	GCTGGTCGAGCGGGATCT	87
OsNCED4	GCCGAGACACGCATTGG	GTGAAGGTGGCGACAGCAA	74
OsABA8ox1	AAGCTGGCAAAACCAACATC	CCGTGCTAATACGGAATCCA	146
OsABA8ox2	CTACTGCTGATGGTGGCTGA	CCCATGGCCTTTGCTTTAT	115
OsABA8ox3	AGTACAGCCCATTCCCTGTG	ACGCCTAATCAAACCATTGC	136
OsAmy1A	GATACGACGTCGAACACCTC	CGGATCGGATACAGCTCGTTG	184
OsAmy2A	GCCGATCATCGCACCTCTTC	CGATCCCACATATCAGTGACG	150
OsAmy3A	GAGGGTCATCACCAAGATCG	TGTGTAGCTAGCTTGCGAGC	116
OsAmy3B	GATTGGGACACGGTATGACG	CTGCAGGAACTCTGAGACCG	110
OsAmy3C	AGACTTCCATGTCGTTGCTC	CAGGCACAAAATAGTCCTG	119
OsAmy3D	GTAGGCAGGCTCTCTAGCCT	CCAACGGTTACAAACTGCGTGA	91
OsAmy3E	GAAGGAAGGCCTCAGGGTTC	GCTCGTACACATCTCGCAGCA	153
OsGA20ox1	AATGAGCATGGTGGTGCAGCAGGAGCAG	GTTAACCACCAGGAAGAAGCCGTGCCTC	235
OsGA20ox2	TACTACAGGGAGTTCTTCGCGGACAGCA	TGTGCAGGCAGCTCTTATACCTCCCGTT	268
OsGA3ox1	GACGATTCACCTCAACATGTTCCCT	GGCTCTGCAGGATGAAGGTGAA	104
OsGA3ox2	TCTCCAAGCTCATGTGGTCCGAGGGCTA	TGGAGCACGAAGGTGAAGAAGCCCGAGT	346
OsABI3	GAAGACGGACAAGAACCTGC	ATGTTCCACACCTGAGACGT	177
OsABI4	TTCCATCACCAACCGTTC	TTGAGGAAGAGATCGAACCA	353
OsABI5	ATGGGATCTGGCATGGTCAA	CAATCGCCATCCCGTTGTAC	151
OsLEA3	GCCGTGAATGATTTCCCTTTG	CACACCCGTCAGAAATCCTCC	148
OsSLR1	GATCGTCACCGTGGTAGAGC	GAGGGAATCGAACATGGTGG	103
OsGAMYB	GAATCCACCCCTCCTGTT	GCCCCATTACTTGCTCTCC	106
OsKO2	CGTGGAGAGGCAAAGACATG	GCCAACAATGAGCTGGAACA	116
OsKS1	TCTCATTTGCACTGGGACCT	ACTGTTCAGCTTTCCCTCCA	182
OsPDS	AAACCGTTCAATGCTGGAGTTG	CAAGGTTCACAGTCCGGGATAG	210
OsZDS	CAATCATTGTACCGCGAGTCAC	TCGACAATGAGCTTCTTTCGGA	209
OsCRTISO	GCTGAAGTCCCTTGAGGAGCCTC	CCCCCGAAGTGCCTATCGCAC	240
UBQ10	TGGTCAGTAATCAGCCAGTTTGG	GCACCACAAATACTTGACGAACAG	81

基因 Gene	上游引物 Forward primer (5'→3')	下游引物 Reverse primer (5'→3')	产物长度 Product length/bp
OsNCED1	AGCCTCGGTCTTCCAATTTT	CACCCAACACAAAAGCTACG	125
OsNCED2	TCATTCCAAAACACCTTCCA	TCCGGGGACCTCCTATGTAT	113
OsNCED3	ACGTGATCAAGAAGCCGTACCT	GCTGGTCGAGCGGGATCT	87
OsNCED4	GCCGAGACACGCATTGG	GTGAAGGTGGCGACAGCAA	74
OsABA8ox1	AAGCTGGCAAAACCAACATC	CCGTGCTAATACGGAATCCA	146
OsABA8ox2	CTACTGCTGATGGTGGCTGA	CCCATGGCCTTTGCTTTAT	115
OsABA8ox3	AGTACAGCCCATTCCCTGTG	ACGCCTAATCAAACCATTGC	136
OsAmy1A	GATACGACGTCGAACACCTC	CGGATCGGATACAGCTCGTTG	184
OsAmy2A	GCCGATCATCGCACCTCTTC	CGATCCCACATATCAGTGACG	150
OsAmy3A	GAGGGTCATCACCAAGATCG	TGTGTAGCTAGCTTGCGAGC	116
OsAmy3B	GATTGGGACACGGTATGACG	CTGCAGGAACTCTGAGACCG	110
OsAmy3C	AGACTTCCATGTCGTTGCTC	CAGGCACAAAATAGTCCTG	119
OsAmy3D	GTAGGCAGGCTCTCTAGCCT	CCAACGGTTACAAACTGCGTGA	91
OsAmy3E	GAAGGAAGGCCTCAGGGTTC	GCTCGTACACATCTCGCAGCA	153
OsGA20ox1	AATGAGCATGGTGGTGCAGCAGGAGCAG	GTTAACCACCAGGAAGAAGCCGTGCCTC	235
OsGA20ox2	TACTACAGGGAGTTCTTCGCGGACAGCA	TGTGCAGGCAGCTCTTATACCTCCCGTT	268
OsGA3ox1	GACGATTCACCTCAACATGTTCCCT	GGCTCTGCAGGATGAAGGTGAA	104
OsGA3ox2	TCTCCAAGCTCATGTGGTCCGAGGGCTA	TGGAGCACGAAGGTGAAGAAGCCCGAGT	346
OsABI3	GAAGACGGACAAGAACCTGC	ATGTTCCACACCTGAGACGT	177
OsABI4	TTCCATCACCAACCGTTC	TTGAGGAAGAGATCGAACCA	353
OsABI5	ATGGGATCTGGCATGGTCAA	CAATCGCCATCCCGTTGTAC	151
OsLEA3	GCCGTGAATGATTTCCCTTTG	CACACCCGTCAGAAATCCTCC	148
OsSLR1	GATCGTCACCGTGGTAGAGC	GAGGGAATCGAACATGGTGG	103
OsGAMYB	GAATCCACCCCTCCTGTT	GCCCCATTACTTGCTCTCC	106
OsKO2	CGTGGAGAGGCAAAGACATG	GCCAACAATGAGCTGGAACA	116
OsKS1	TCTCATTTGCACTGGGACCT	ACTGTTCAGCTTTCCCTCCA	182
OsPDS	AAACCGTTCAATGCTGGAGTTG	CAAGGTTCACAGTCCGGGATAG	210
OsZDS	CAATCATTGTACCGCGAGTCAC	TCGACAATGAGCTTCTTTCGGA	209
OsCRTISO	GCTGAAGTCCCTTGAGGAGCCTC	CCCCCGAAGTGCCTATCGCAC	240
UBQ10	TGGTCAGTAATCAGCCAGTTTGG	GCACCACAAATACTTGACGAACAG	81

Dynamic profile of genes related to seed dormancy under high humidity condition during late stage of rice grain filling

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