Abstract: In order to investigate the molecular mechanism of Vc accumulation, 661 bp cDNA encoding L-galactono-1,4-lacton dehydrognease (GalLDH) fragment was cloned from fruit of Actinidia eriantha by the method of RT-PCR, and then translated into amino acid sequences. In GenBank, compared with Actinidia delicisa, the amino acid sequence of GalLDH gene sequence showed 99% identity with those of Actinidia delicisa and they were both classified in the same family. The amino acid sequence of GalLDH gene sequence showed 91% identity with Vitis vinifera and more than 80% identity with Fragaria xananassa, Rosa roxburghii, Ipomoea batatas, citrur unshiu, Arabidopsis thaliana and Cucumis melo. These results suggested that the cDNA fragment cloned in this stady was from GalLDH gene of Actinidia eriantha．

Key words: kiwifruit, L-galactono-1, 4-lacton dehydrognease (GalLDH), ascorbic acid (AsA), gene cloning