Abstract: Changes of five extracellular enzymes activity(carboxymethyl cellulose, hemicellulase, laccase, neutral proteinase and acid phosphatase) of Flammulina velutipes were investigated during its growth and development. The results showed that the activity curves of extracellular enzyme during the growth and development showed markedly periodic style. Carboxymethyl cellulose activity was relatively low during the mycelial vegetative growth phase but it reached 11-43 U after primordium formation. Hemicellulase activity was the highest of 8-13 U during the harvest phase. Laccase activity was relatively high during mycelial growth phase and decreased to 0-15 U after primordium formation. Neutral proteinase activity remained constant during mycelial vegetative growth phase, decreased to relatively low levels before primordium formation, and increased again during the fruit body growth phase. Acid phosphatase activity decreased with mycelium culture, but increased sharply after development formation.

Key words: Flammulina velutipes, growth and development, extracellular enzyme, culture material