Abstract: To elucidate the genetic diversity of wheat breeding parents in southern Zhejiang province， 88 SSR markers located on 21 chromosomes of wheat were amplified to study the genetic diversity among 48 wheat cultivars. Stable polymorphism could be amplified by 27 out of 88 SSR markers among 48 wheat cultivars. A total of 159 alleles were identified in these varieties. Each of primer pairs could detect 2 to 9 alleles，with an average of 5.89.The PIC value was in the range from 0.117 to 0.850， with the average of 0.652.The genetic similarity coefficients of the 48 wheat cultivars ranged from 0.68 to 1.00， which suggested that the diversity of these 48 wheat cultivars was lower. Furthermore， all the cultivars were classified into 6 groups at the level of genetic similarity coefficient of 0.75 by un-weighted pair-group method with arithmetic mean. The results of grouping can reveal the genetic relationship of cultivars.

Key words: wheat, SSR markers, genetic diversity