Abstract: Amplified fragment length polymorphism (An )analysis of eight prunella germplasm resources was carried out to explore a variety of the genetic diversity．Using AFLP markers, 32 pairs of selective amplification primers was screened out．The similarity among the pnmeHa germplasm was analyzed and phylogenetic tree was constructed ．The results showed that：(1)genomie DNA extracted from prune~a by SDS method was good to satisfy AFLP analysis；(2)10 m rs of primers with stronger polymorphism，better banding and higher resoluing power were screened out from 32 pairs of selective amplification primers；(3)divided eight germplasms into three categories through constructing evolutionary tree；(4)Through the construction of evdutionary tree，the eight germplasms were divided into three categories．Prunella germplasm resources are rich in genetic diversity，certain morphological characteristics and have relationship with genotype．

Key words: Prunella vulgaris Linn, germplasm resourse, AFLP, genetic diversity