Identification, evolution and expression analysis of Pht1 gene family in Sorghum bicolor

doi:10.3969/j.issn.1004-1524.2017.01.03

›› 2017, Vol. 29 ›› Issue (1): 16-22.DOI: 10.3969/j.issn.1004-1524.2017.01.03

• Crop Science • Previous Articles Next Articles

Identification, evolution and expression analysis of Pht1 gene family in Sorghum bicolor

YU Chenliang¹, ZHANG Chenghao¹, ZHAN Yihua², DONG Wenqi^{1, *}

1. Institute of Vegetable, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China;
2. College of Life Sciences, Zhejiang University, Hangzhou 310058, China

Received:2016-08-08 Online:2017-01-15 Published:2017-02-23

Abstract

Abstract: Phosphorus is a significant component of biological molecules, and it is also an important nutrient for plant growth. To identify putative Pht1 family genes in S. bicolor, we performed BLAST searches against the S. bicolor genome database Phytozome using the well-characterized Arabidopsis AtPht and rice OsPht1 family as queries, and 11 non-redundant putative SbPht1 genes were identified. Information of gene sequence, gene structure, protein sequence and chromosomal location were downloaded from Phytozome database. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to analyze the expression patterns of 11 SbPht1 genes under phosphorus deficiency and high phosphorus treatment. The results showed that most of the SbPht1 genes expression was increased under the condition of phosphorus deficiency. Under high phosphorus treatment, the expression of SbPT1, SbPT4, SbPT6, SbPT7, SbPT8, SbPT9 and SbPT10 was up-regulated in the roots. In the shoots, the expression of SbPT3 and SbPT5 was increased. Through the analysis of phosphate transport protein from the whole genome of sorghum, this paper provides the molecular biological basis for the study of phosphorus, and has a great significance in the improvement of plant absorption and utilization of phosphorus.

Key words: sorghum, phosphorus, phosphate transporter family, gene expression

CLC Number:

S514

YU Chenliang, ZHANG Chenghao, ZHAN Yihua, DONG Wenqi. Identification, evolution and expression analysis of Pht1 gene family in Sorghum bicolor[J]. , 2017, 29(1): 16-22.

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Identification, evolution and expression analysis of Pht1 gene family in Sorghum bicolor

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