Effect of salicylic acid and proline on gene expression profiles in response to salt stress in lettuce

doi:10.3969/j.issn.1004-1524.2017.09.10

›› 2017, Vol. 29 ›› Issue (9): 1489-1497.DOI: 10.3969/j.issn.1004-1524.2017.09.10

• Horticultural Science • Previous Articles Next Articles

Effect of salicylic acid and proline on gene expression profiles in response to salt stress in lettuce

WU Jianhua^{1, 2}, WU Zhongxia³, WANG Yuanhua^{1, 2}, FENG Yingna^{1, 2, *}, YAN Zhiming^{1, 2}, CAI Shanya¹, WANG Quanzhi^{1, 2}, SUN Ying¹

1. Jiangsu Polytechnic College of Agriculture and Forestry, Zhenjiang 212400, China;
2. Jiangsu Engineering and Technology Center for Modern Horticulture, Zhenjiang 212400, China;
3. Suining County Gaozuo Town Agricultural Technology Extension Service Center, Xuzhou 221200, China

Received:2017-03-04 Online:2017-09-20 Published:2017-09-27

Abstract

Abstract: In order to investigate the role of salicylic acid (SA) and proline (Pro) in alleviating salt stress in plants, a lettuce cultivar ‘Meiguodayesusheng' was used in this study. The lettuce seedlings were grown in hydroponic system under salt stress, and SA and Pro were added to the nutrition solution, respectively. In determination of the morphological and physiological indexes related to salt stress, the results showed that the growth of lettuce seedlings under salt stress was significantly alleviated when treated with 0.2 mmol·L^-1 SA and 0.2 mmol·L^-1 Pro for 24 hours. The expression levels of the genes related to salt stress were analyzed by RT-PCR and qRT-PCR,and the expression of LSNCED1, LSNCED2 and LSP5CS were remarkably up-regulated under salt stress. These results indicated that 0.2 mmol·L^-1 SA and 0.2 mmol·L^-1 Pro could reduce the injury to lettuce caused by salt stress to some extent.

Key words: salt stress, lettuce, physiological characteristic, salt-stress related gene, gene expression

CLC Number:

S636.2

WU Jianhua, WU Zhongxia, WANG Yuanhua, FENG Yingna, YAN Zhiming, CAI Shanya, WANG Quanzhi, SUN Ying. Effect of salicylic acid and proline on gene expression profiles in response to salt stress in lettuce[J]. , 2017, 29(9): 1489-1497.

References

[1] 祁向玲. 外源ALA、SA、CaCl 2 处理对盐胁迫下生菜植株生理特性的影响[D].杨凌:西北农林科技大学, 2008.
QI X L. The affection of exogenous ALA, SA, CaCl 2 on physiological characteristic of lettuce under salt stress[D]. Yangling: Northwest A & F University, 2008. (in Chinese with English abstract)
[2] 何俊瑜,任艳芳.外源水杨酸对NaCl胁迫下生菜幼苗生长和光合性能的影响[J].湖南农业大学学报(自然科学版),2009,35(6):628-631.
HE J Y, REN Y F. Effects of exogenous salicylic acid on growth and photosynthetic characteristics of lettuce ( Lactuca sativa L.) seedlings under NaCl stress[J]. Journal of Hunan Agricultural University ( Natural Sciences ), 2009, 35(6):628-631.(in Chinese with English abstract)
[3] BORSANI O, VALPUESTA V, BOTELLA M A. Evidence for a role of salicylic acid in the oxidative damage generated by NaCl and osmotic stress in Arabidopsis seedlings[J]. Plant Physiology ,2001,126(3):1024-1030.
[4] 李德红,潘瑞炽.水杨酸在植物体内的作用[J].植物生理学通讯,1995,31(2):144-149.
LI D H, PAN R Z. The role of salicylic acid in plant[J]. Plant Physiology Communication ,1995,31(2),144-149. (in Chinese with English abstract)
[5] 刘爱华,王永飞.土壤水分胁迫对生菜幼苗部分生理指标的影响[J].西北农业学报,2010,19(6):144-147.
LIU A H, WANG Y F. Effects of soil water stress on the physiological and biochemical indexes of Lactuca sativa L. during seedling stage[J]. Acta Agriculturae Boreali-occidentalis Sinica , 2010,19(6):144-147. (in Chinese with English abstract)
[6] 祁向玲, 邹志荣. NaCl胁迫对两品种生菜生理特性的影响[J].仲恺农业技术学院学报, 2008,21(2):14-18.
QI X L, ZOU Z R. Effects of sodium chloride stress on physiological traits of lettuce[J]. Journal of Zhongkai University of Agriculture and Technology 2008,21(2):14-18. (in Chinese with English abstract)
[7] 李锡香,王海平.莴苣种质资源描述规范和数据标准[M].北京:中国农业科技出版社, 2006:4-30.
[8] SAWADA Y, AOKI M, NAKAMINAMI K, et al. Phytochrome-and gibberellin-mediated regulation of abscisic acid metabolism during germination of photoblastic lettuce seeds[J]. Plant Physiology , 2008, 146(3):1386-1396.
[9] PORCEL R, AZCÓN R, RUIZ-LOZANO J M, et al. Evaluation of the role of genes encoding for delta1—pyrroline-5-carboxylate synthetase ( P 5 CS ) during drought stress in arbuscular mycorrhizal Glycine max and Lactuca sativa plants[J]. Physiological and Molecular Plant Pathology ,2004,65(4): 211-221.
[10] HE Z, LI J, ZHANG H, et al. Different effects of calcium and lanthanum on the expression of phytochelatin synthase gene and cadmium absorption in Lactuca sativa [J]. Plant Science ,2005, 168(2):309-318.
[11] MATSUI K, TANAKA H, OHME-TAKAGI M. Suppression of the biosynthesis of proanthocyanidin in Arabidopsis by a chimeric PAP1 repressor[J]. Plant Biotechnology Journal ,2004,6 (2): 487-493.
[12] PARK K I, CHOI J D, HOSHINO A, et al. An intragenic tandem duplication in a transcriptional regulatory gene for anthocyanins biosynthesis confers pale-colored flowers and seeds with fine spots in Ipomea tricolor[J]. Plant Journal ,2004,38 (5): 840-849.
[13] LIVAK K J, SCHMITTGEN T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2 -ΔΔCT method[J]. Methods , 2001,25 (4): 402-408.
[14] TESTER M, DAVENPORT R. Na + tolerance and Na + transport in higher plants[J]. Annals of Botany ,2003,91(5):503-527.
[15] TAN B C, SEHWARTZ S H, ZEEVAART J A, et al. Genetic control of abscisis acid biosynthesis in maize[J]. Proceedings of the National Academy of Sci ences of the United States of America ,1997,94(22):12235-12240.
[16] KITAHATA N, HAN S Y, NOJI N, et al. A 9-cis-epoxycarotenoid dioxygenase inhibitor for use in the elucidation of abscisic acid action mechanisms[J]. Bioorganic Medicinal Chemistry ,2006,14(16):5555-5561.
[17] 王学敏,高洪文.柠条锦鸡儿NCED4基因的克隆及对逆境胁迫的响应[C]// 中国遗传学会.植物分子生物学与现代农业——全国植物生物学研讨会论文摘要集.北京,2010.
[18] 陈吉宝,赵丽英,景蕊莲,等.植物脯氨酸合成酶基因工程研究进展[J].生物技术通报,2010 (2):8-10.
CHEN J B,ZHAO L Y, JING R L, et al. Advance in genetic engineering of proline sythetase in plant[J]. Biotechnology Bulletin , 2010 (2):8-10. (in Chinese with English abstract)
[19] LEHMANN S, FUNCK D, SZABADOS L, et al. Proline metabolism and transport in plant development[J]. Amino Acids ,2010,39(4):949-962.
[20] KISHOR P, HONG Z, MIAO G H, et al. Overexpression of deltal-pyrroline-5-carboxylate synthetase increases proline production and confers osmotolerance in transgenic plants[J]. Plant Physiology ,1995,108(4):1387-1394.
[21] CHOUDHARY N L, SAIRAM R K, TYAGI A. Expression of deltal-pyrroline-5-carboxylate synthetase gene during drought in rice( Oryza sativa L.)[J]. Indian Journal of Biochemistry & Biophysics ,2005,42(6):366-370.
[22] HMIDA-SAYYARI A, GARGOURI-BOUZID R, BIDANI A, et al. Overexpression of deltal-pyrroline-5-carboxylate synthetase increases proline production and confers salt tolerance in transgenic potato plants[J]. Plant Science ,2005,169(4):746-752.
[23] 陈吉宝,赵丽英,毛新国,等.转 PvP 5 CS 1基因拟南芥植株对干旱和盐胁迫的反应[J].作物学报,2010,36(1):147-153.
CHEN J B, ZHAO L Y, MAO X G, et al. Response of PvP 5 CS 1 transgenic Arabidopsis plants to drought-and salt-stress[J]. Acta Agronomica Sinica , 2010,36(1):147-153. (in Chinese with English abstract)
[24] BURBIDGE A, GRIEVE T M, JACKSON A, et al. Characterization of the ABA-deficient tomato mutant notabilis and its relationship with maize Vp14[J]. Plant Journal ,1999,17(4):427-431.
[25] IUCHI S, KOBAYASHI M, TAJI T, et al. Regulation of drought tolerance by gene manipulation of 9-cis-epoxycarotenoid dioxygenase,a key enzyme in abscisic acid biosynthesis in Arabidopsis[J]. Plant Journal ,2001,27(4):325-333.
[26] DELAUNEY A J, MORISHITA H, VERMA D P S. Proline biosynthesis and osmoregulation in plants[J]. Plant Journal ,1993,4(2):215-223.
[27] 田亚然,薛璟祺,赵家昱,等.叶子花脱落酸生物合成关键酶基因 NCED 的克隆及调节开花功能初探[J].西北植物学报,2015,35(6):1106-1112.
TIAN Y R,XUE J Q,ZHAO J Y, et al. Isolation of NCED, the key ABA biosynthesis gene and its function analysis in flowering regulation of Bougainvillea glabra [J]. Acta Botanica Boreali-Occidentalia Sinica , 2015, 35(6):1106-1112. (in Chinese with English abstract)
[28] 李康,聂小军,方桂英,等.普通小麦及其近缘种 NCED 基因的克隆及表达分析[J].西北农业学报,2010,19(6):55-59.
LI K, NIE X J, FANG G Y, et al. Cloning and expression of a NCED gene in wheat and its relative species[J]. Acta Botanica Boreali-Occidentalia Sinica , 2010, 19(6):55-59. (in Chinese with English abstract)
[29] 刘江那,邓晓燕,李志博,等.棉花9-顺式环氧类胡萝卜素双加氧酶基因在干旱条件下的表达分析[J].石河子大学学报(自然科学版),2010, 28 (5):546-550.
LIU J N, DENG X Y, LI Z B, et al. The expression analysis of cotton 9-cis-epoxycarotenoid dioxygenase gene under drought stress[J]. Journal of Shihezi University ( Natural Science ) , 2010, 28 (5):546-550. (in Chinese with English abstract)
[30] SILVA-ORTEGA C O, OCHOA-ALFARO A E, REYES-AGÜERO J A, et al. Salt stress increases the expression of p5cs gene and induces proline accumulation in cactus pear[J]. Plant Physiology and Biochemistry ,2008,46(1):82-92.
[31] 陈吉宝,景蕊莲,毛新国,等.普通菜豆PvP5CS2基因对逆境胁迫的应答[J].作物学报,2008,34(7):1121-1127.
CHEN J B, JING R L, MAO X G, et al. A response of PvP5CS2 gene to abiotic stresses in common bean[J]. Acta Agronomica Sinica , 2008,34(7):1121-1127. (in Chinese with English abstract)
[32] 张积森,陈由强,张木清,等.甘蔗△ 1 -吡咯琳-5-羧酸合成酶基因( P 5 CS )基因的克隆与序列分析[J].热带作物学报,2009,30(9):1337-1344.
ZHANG J S,CHEN Y Q,ZHANG M Q, et al. Molecular cloning and expression of the P5CS gene from sugarcane[J]. Chinese Journal of Tropical Crops , 2009,30(9):1337-1344. (in Chinese with English abstract)
[33] 董慧,段小春,常智慧.外源水杨酸对多年生黑麦草耐盐性的影响[J].北京林业大学学报,2015,37(2):128-135.
DONG H, DUAN X C, CHANG Z H. Effect of exogenous salicylic acid on tolerance in perennial ryegrass[J]. Journal of Beijing forestry university ,2015,37(2):128-135. (in Chinese with English abstract)
[34] 沙汉景.外源脯氨酸对盐胁迫下水稻耐盐性的影响[D]. 哈尔滨: 东北农业大学,2013.
SHA H J. Effect of exogenous proline on the salt-tolerance of rice ( Oryza sativa L.) [D]. Harbin: Northeast Agricultural University, 2013. (in Chinese with English abstract)
[35] AHMED C B, MAGDICH S, ROUINA B B, et al. Exogenous proline effects on water relations and ions contents in leaves and roots of young olive[J]. Amino Acids ,2011,40(2):565-573.
[36] 王玉萍,董雯,张鑫,等.水杨酸对盐胁迫下花椰菜种子萌发及幼苗生理特性的影响[J].草业学报,2012,21(1):213-219.
WANG Y P,DONG W,ZHANG X, et al. Effects of salicylic acid on seed germination and physiological characters of cauliflower seedlings under salt stress[J]. Acta Prataculturae Sinica ,2012,21(1):213-219. (in Chinese with English abstract)

Effect of salicylic acid and proline on gene expression profiles in response to salt stress in lettuce

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	LU Anqiao, ZHANG Fengju, WANG Xueqin, XU Xing. Effects of NaCl and Na₂SO₄ stress on content and distribution of K ⁺ and Na ⁺ of Echinochloa frumentacea seedlings [J]. Acta Agriculturae Zhejiangensis, 2021, 33(3): 396-403.
[2]	HUANG Yongming, SONG Fang, WANG Ce, YAO Jinglei, WANG Zhijing, HE Ligang, WU Liming, JIANG Yingchun. Effects of root pruning on growth and expression of related genes in Poncirus trifoliata [J]. Acta Agriculturae Zhejiangensis, 2021, 33(2): 270-277.
[3]	XU Xiuhong, LIU Jinliang, LI Dongcheng, LIU Renxiang. Analysis of nicotine content and related gene expression in different types of tobacco germplasm [J]. , 2020, 32(9): 1555-1563.
[4]	SONG Xindan, CHEN Binbin, MA Zengling, XU Lili, LIN Lidong, WU Mingjiang. Effects of salinity level on photosynthetic characteristics of Sargassum fusiforme seedlings [J]. , 2020, 32(9): 1634-1644.
[5]	LIU Kunju, ZHANG Xiaohui, PANG Youzhi, ZHAO Shujuan, QI Yanxia, WANG Qiankun. Relationship of plumage color with expression and polymorphism of GNAS gene in Korean quail [J]. , 2020, 32(8): 1369-1377.
[6]	SHI Jing, LIU Dongyang, ZHANG Fenghua. Physiological response and salt tolerance mechanism of cotton seedlings to salt stress [J]. , 2020, 32(7): 1141-1148.
[7]	LI Qiuling, QI Ying, WANG Chen, ZHANG Yiming, WANG Xinyu, SHANG Xiaolan, JIA Yonghong, LI Meiru, CHU Mingxing. Effect of heat stress on gene expressions and signaling pathways of mammary gland in Chinese Holstein [J]. , 2020, 32(5): 770-778.
[8]	LU Yi, GAO Youling, WANG Shuitao, HE Shengsheng. Effects of microRNA-499 on lipid metabolism-related gene expression in Pelodiscus sinensis [J]. , 2020, 32(5): 798-803.
[9]	LI Weifang, WANG Chunlei, WANG Ni, DENG Yuzheng, YAO Yandong, WEI Lijuan, LIAO Weibiao. Research progress on effect of nitric oxide on adventitious root formation in plants [J]. , 2020, 32(4): 742-752.
[10]	WU Yan, QIAO Xiaoyan, GE Weiqiang, GAO Qinghai. Effects of exogenous melatonin on physiological and biochemical characteristics in female flowers of Trichosanthes kirilowii under high temperature and strong light [J]. , 2020, 32(3): 421-429.
[11]	QIN Ling, ZHANG Xin, RONG Chunxiao, MO Chuanyuan, FAN Lu, YAN Jie, MENG Ying, ZHANG Manrang. Identification and expression analysis of polyamine oxidase (PAO) gene family in apple [J]. , 2020, 32(2): 262-273.
[12]	ZHANG Zheng, WANG Xiaorong, QIAN Hong, ZHANG Lan, YAN Peng, ZHANG Liping, ZHANG Xinfu, LI Xin, HAN Wenyan. Effects of anthracnose disease on photosynthetic characteristics in tea leaves (Camellia sinensis L.) [J]. , 2020, 32(11): 2020-2026.
[13]	BIAN Jianwen, CUI Yan, YANG Songqi, LUO Guanghong, MENG Xiangang. Effects of Chlamydomonas debaryana Gor. and Anabaena azotica Ley. on wheat seedling growth under salt stress [J]. , 2020, 32(10): 1748-1756.
[14]	ZHU Xiaolin, WEI Xiaohong, WANG Baoqiang, WANG Xian, ZHANG Mingjun. Transcriptome analysis of tomato under salt stress induced by c-GMP [J]. , 2020, 32(10): 1788-1797.
[15]	ZHAI Keqing, ZHANG Xinyu, ZHOU Niannian, AN Yulan, LEI Yue, GAO Jie, GAN Defang, LIU Wen, HE Xiaomei. Changes of physiological indexes and quality analysis of lettuce under optical interference film [J]. , 2019, 31(9): 1493-1501.