›› 2018, Vol. 30 ›› Issue (1): 26-35.DOI: 10.3969/j.issn.1004-1524.2018.01.04

• Animal Science • Previous Articles     Next Articles

Isolation, identification and drug sensitive test of Trichosporon loubieri from beef cattle

ZHONG Rui1, JIANG Yaozhang1, MA Xiaoping1, *, ZUO Zhicai1, HUANG Xiaoli2, DENG Junliang1, SHEN Liuhong1, YU Shumin1   

  1. 1. College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China;
    2. College of Animal Science and Technology,Sichuan Agricultural University, Chengdu 611130, China
  • Received:2017-07-31 Online:2018-01-20 Published:2018-02-09

Abstract: In order to have a deeper understanding of the causes of skin disease that beef cattle suffered and find the effective treatment, we collected the dandruff and hair of beef cattle skin with dermatophytosis then carried our an inspection and a separation of mycology. The dandruff and hair of beef cattle skin with dermatophytosis were collected and used for fungi culture. The morphological characteristics and IGS sequences of the isolated strains were identified. The pathogenicity test was carried out in mice, and drug sensitive test was performed. Finally, one strain of fungi, zrfg01, was obtained. The length of the IGS sequence was 542 bp amplified by PCR, which was submitted to GenBank (ID, MF401393.1). NCBI blast result showed that zrfg01 had 100% similarity with Trichosporon loubieri. According to the morphological identification, IGS sequence analysis and phylogenetic tree, the isolated strain was identified as T. loubieri. The pathogenicity test revealed that the fungus had a selective pathogenesis to mice. The drug susceptibility test showed that under the environment of 25 ℃, T. loubieri was resistant to terbinafine and fluorouracil, but sensitive to clotrimazole, amphotericin B, fluconazole and voriconazole. This study provided an important reference for the diagnosis and treatment of dermomycosis caused by T. loubieri.

Key words: Trichosporon loubieri, morphology, ITS sequence, IGS sequence, pathogenicity, drug sensitivity.

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