Identification and biological characteristics analysis of Moraxella from swine

doi:10.3969/j.issn.1004-1524.2018.01.05

›› 2018, Vol. 30 ›› Issue (1): 36-42.DOI: 10.3969/j.issn.1004-1524.2018.01.05

• Animal Science • Previous Articles Next Articles

Identification and biological characteristics analysis of Moraxella from swine

LIAO Changyu¹, ZHANG Pengfei^{1, 2}, WANG Yin^{1, 2, *}, YANG Zexiao¹, YAO Xueping¹, JIANG Ruijiao¹, WU Xulong^{1, 2}, ZHANG Bo^{1, 2}, ZHOU Lijun^{1, 2}, SONG Yong³

1. College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China;
2. Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, China;
3. Ruipu Biological Co., Ltd, Tianjin 300300, China

Received:2017-05-28 Online:2018-01-20 Published:2018-02-09

Abstract

Abstract: A gram-negative bacterium was isolated from the heart of dead piglets, and morphological observation, biochemical identification and 16S rDNA sequence were performed. The strain was identified as Moraxella porci sp., which was named as Moraxella porci-ZY20001. The pathogenicity test, drug susceptibility test and partial resistance gene were detected in strain ZY20001. The results showed that the bacteria caused the pleural effusion in mice and showed varying degrees of sensitivity to erythromycin, penicillin, polymyxin and other antibiotics (a total of 21 kinds), except piperacillin. The strain ZY20001 had a TEM-type β-lactamase-resistant gene, and the phenotype was not consistent with the genotype. This study provided scientific reference for the further study of the evolution and drug therapy of Moraxella, and the analysis of relationship between penicillin resistance phenotype and genotype would enrich the epidemiological survey data.

Key words: Moraxella, isolation and identification, 16S rDNA, drug resistance

CLC Number:

LIAO Changyu, ZHANG Pengfei, WANG Yin, YANG Zexiao, YAO Xueping, JIANG Ruijiao, WU Xulong, ZHANG Bo, ZHOU Lijun, SONG Yong. Identification and biological characteristics analysis of Moraxella from swine[J]. , 2018, 30(1): 36-42.

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Identification and biological characteristics analysis of Moraxella from swine

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