Expression of antimicrobial peptides thanatin tandem fusion in Escherichia coli

doi:10.3969/j.issn.1004-1524.2018.05.18

›› 2018, Vol. 30 ›› Issue (5): 804-809.DOI: 10.3969/j.issn.1004-1524.2018.05.18

• Plant Protection • Previous Articles Next Articles

Expression of antimicrobial peptides thanatin tandem fusion in Escherichia coli

LIU Wei¹, LI Qinghai², LIU Renhu^3,*

1.Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China;
2. Department of Radiology, The Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou 310009, China;
3. Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Received:2017-09-07 Online:2018-05-20 Published:2018-05-23

Abstract

Abstract: The antibacterial peptide thanatin is a broad spectrum cationic antibacterial peptide, which can significantly kill pathogenic fungi. In order to improve the expression level of thanatin, three copies of thanatin monomer and MBP protein was co-expressed in Escherichia coli. The expression products were identified by Western blot and mass spectrometry after enzyme digestion and purification. The results showed that the three copies of thanatin fusion protein, 3×thanatin, were successfully expressed in E. coli and had good inhibition activity on Sclerotinia sclerotiorum. The minimum concentration of inhibition on S. sclerotiorum was 6.6 μmol·L^-1 for 3×thanatin, which was about ten times higher than 1×thanatin.

Key words: antimicrobial peptides, thanatin, tandem, expression

CLC Number:

S432.2⁺3

LIU Wei, LI Qinghai, LIU Renhu. Expression of antimicrobial peptides thanatin tandem fusion in Escherichia coli[J]. , 2018, 30(5): 804-809.

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Expression of antimicrobial peptides thanatin tandem fusion in Escherichia coli

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