›› 2018, Vol. 30 ›› Issue (9): 1513-1518.DOI: 10.3969/j.issn.1004-1524.2018.09.10

• Horticultural Science • Previous Articles     Next Articles

Isolation, drug resistance of Klebsiella pneumoniae from waxberry and its virulence gene analysis

DAI Baoling1, 2, YANG Hua2, DAI Xianjun1, YANG Guiling2, WANG Wen2, XIAO Yingping2, *   

  1. 1. College of Life Science, China Jiliang University, Hangzhou 310018, China;
    2. Institute of Quality and Standard for Agro-Products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
  • Received:2017-09-27 Online:2018-09-25 Published:2018-10-15
  • Contact: 肖英平,E-mail: ypxiaozju@126.com
  • Supported by:
    浙江省植物有害生物防控省部共建国家重点实验室培育基地项目(2010DS700124-ZM1608)

Abstract: Waxberry is easy to be polluted by pathogenic bacteria in the process of production and transportation, the main purpose, therefore, is to analyze the carrying status, drug resistance characteristics and virulence genes of Klebsiella pneumoniae in waxberry. 90 samples were collected from Hangzhou market and base. To determine coliform contamination and Klebsiella pneumoniae, the bacteria isolated from the samples were identified by Vitek Auto Microbic System and 16S rRNA sequence. The resistant phenotype of Klebsiella pneumoniae was analyzed by VITEK 2 negative bacteria susceptibility card. Drug resistance genes and virulence genes were detected by PCR amplification. The results showed as follows: 51 samples were contaminated by coliforms in the 90 samples, and the pollution rate was 56.7%; 9 strains of Klebsiella pneumonia were detected, the positive rate was 10%, of which 8 strains were clustered into a cluster with high homology, and the homology of another was significantly different. These 9 strains of Klebsiella pneumonia showed different resistance to ampicillin, cefazolin and nitrofurantain. The resistant rate to ampicillin reached 88.89%. tetA gene was detected in all the 9 strains, and positive rate of drug resistance gene blaTEM, gyrA, aadA1 and AAC(6')-1b were 88.89%, while blaCTX-M, floR, ereA, ermB and mecA gene were not detected. The virulence genes of wcaG, magA, rmpA and Aerobactin were not detected. Qualitative analysis showed that waxberry had a certain contamination of Klebsiella pneumoniae, but these strains did not carry virulence genes such as wcaG, magA, rmpA and Aerobactin.

Key words: waxberry, Klebsiella pneumoniae, drug resistant, virulence

CLC Number: