Identification and expression analysis of PAL gene family in apple

doi:10.3969/j.issn.1004-1524.2018.12.07

›› 2018, Vol. 30 ›› Issue (12): 2031-2043.DOI: 10.3969/j.issn.1004-1524.2018.12.07

• Horticultural Science • Previous Articles Next Articles

Identification and expression analysis of PAL gene family in apple

ZHANG Lizhi, FAN Sheng, AN Na, ZUO Xiya, GAO Cai, ZHANG Dong^*, HAN Mingyu

College of Horticulture, Northwest A&F University, Yangling 712100, China

Received:2018-03-09 Online:2018-12-25 Published:2018-12-28

Abstract

Abstract: Based on apple genome and the genomic database of Golden Delicious, 8 apple PAL genes were identified through BLAST tool. All members shared conserved MIO active site including Ala-Gly-Ser. The analysis of classification and evolutionary relationship showed that the 8 MdPAL genes were divided into three groups, the molecular weight was between 47.713 and 81.748 ku, the isoelectric point was between 5.44 and 6.39, and the members of one subgroup shared similar gene structure and conserved protein motifs. Based on GEO database and qRT-PCR, expression patterns of MdPAL genes in different organs and different stages of fruits in Nagafu No.2 were determined. The analysis of expression patterns of MdPAL genes in different organs showed that MdPAL2, MdPAL3/4/6/7, MdPAL8 had the highest expression levels in leaves, while MdPAL1 and MdPAL5 expressions were the highest in flowers. The expression patterns were diverse. In 5 stages of the fruit development in Nagafu No.2, the expression patterns of MdPAL genes were significantly increased in the young stage or the ripening stage except MdPAL1, indicating that they might play important roles in different stages during the fruit development.

Key words: apple, PAL gene family, bioinformatics, phylogenetic analysis, gene expression

CLC Number:

S661.1

ZHANG Lizhi, FAN Sheng, AN Na, ZUO Xiya, GAO Cai, ZHANG Dong, HAN Mingyu. Identification and expression analysis of PAL gene family in apple[J]. , 2018, 30(12): 2031-2043.

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Identification and expression analysis of PAL gene family in apple

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