Effect and correlation analysis of Eimeria tenella infection on IL-6, IL-8 and CCLi2 genes expression in spleen and caecum of Jinghai Yellow Chicken (Gallus gallus)

doi:10.3969/j.issn.1004-1524.2019.01.05

›› 2019, Vol. 31 ›› Issue (1): 39-46.DOI: 10.3969/j.issn.1004-1524.2019.01.05

• OAnimal Science • Previous Articles Next Articles

Effect and correlation analysis of Eimeria tenella infection on IL-6, IL-8 and CCLi2 genes expression in spleen and caecum of Jinghai Yellow Chicken (Gallus gallus)

XIN Shijie^1,2, WANG Xiaohui^1,2, DAI Guojun^1,2,*, AN Tingting^1,2, ZHANG Tao^1,2, ZHANG Genxi^1,2, XIE Kaizhou^1,2, WANG Jinyu^1,2, WANG Hongsheng³

1. College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China;
2. Key Lab for Animal Genetics, Breeding and Reproduction and Molecular Design of Jiangsu Province, Yangzhou 225009, China;
3. Jiangsu Jinghai Poultry Group Co. Ltd., Haimen 226100, China

Received:2018-03-14 Online:2019-01-25 Published:2019-04-09

Abstract

Abstract: Interleukin 6 (IL-6), interleukin 8 (IL-8) and CC motif chemokine ligand 2 (CCLi2) play an important role in the inflammatory response and immune. In this study, in order to reveal the expression relationships between the three genes in spleen, caecum and between spleen and caecum at coccidiosis infected and non-infected state, Jinghai Yellow Chicken(Gallus gallus) were divided into coccidiosis infection and non-infection group, the relative expression of mRNA of IL-6, IL-8 and CCLi2 in spleen and caecum was detected by quantitative real-time polymerase chain reaction (qRT-PCR), the relative expression difference of the three genes were compared between infection and control group in the same tissue, meanwhile, the relative expression correlation were analyzed among the three genes in the same and different tissues within the same group. The results showed that the relative expression levels of IL-6, IL-8 and CCLi2 genes in spleen tissue of infected group were all higher than those of the control group (P<0.05), and the relative expression level of IL-6 gene in infected group was extremely significant different from control group (P<0.01); the relative expression levels of IL-6, IL-8 and CCLi2 genes in the caecum tissue of infected group were all extremely significant different from that of the control group (P<0.01). The correlation analysis showed that the correlation coefficient between the expression of IL-6, IL-8 and CCLi2 genes in spleen tissues of infected group were all positively related (P<0.01); in caecum tissue of infected group, there were extremely significant positive correlations(P<0.01) among the relative expression of the IL-8, CCLi2 with IL-6 genes, but the relative expression correlation between IL-8 and CCLi2 genes was not significant correlated (P>0.05); in the spleen tissue of the control group, there were extremely significant positive correlations (P<0.01) between the relative expression of IL-6, IL-8 with CCLi2 genes, and the correlation between other genes in the control group was not significant (P>0.05); there were extremely significant positive correlations (P<0.01) among the three genes of IL-6, IL-8 and CCLi2 in the caecum tissue of the control group; the correlation analysis results also showed that the expression of the three genes was not significantly correlated (P>0.05) between spleen and caecum tissue, regardless of the infection group or the control group. All of the results indicated that IL-6, IL-8 and CCLi2 might play an important role in the process of Jinghai Yellow Chicken coccidiosis infection, and the results provided the basis for further exploring the role of these three genes in the genetic breeding of coccidiosis resistance, and it had an important significance for Jinghai Yellow Chicken genetic breeding of coccidiosis resistance.

Key words: Jinghai Yellow Chicken, caecum, spleen, IL-6, IL-8, CCLi2, gene expression, correlation analysis

CLC Number:

S831.2

XIN Shijie, WANG Xiaohui, DAI Guojun, AN Tingting, ZHANG Tao, ZHANG Genxi, XIE Kaizhou, WANG Jinyu, WANG Hongsheng. Effect and correlation analysis of Eimeria tenella infection on IL-6, IL-8 and CCLi2 genes expression in spleen and caecum of Jinghai Yellow Chicken (Gallus gallus)[J]. , 2019, 31(1): 39-46.

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Effect and correlation analysis of Eimeria tenella infection on IL-6, IL-8 and CCLi2 genes expression in spleen and caecum of Jinghai Yellow Chicken (Gallus gallus)

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