Effects of microRNA-499 on lipid metabolism-related gene expression in Pelodiscus sinensis

doi:10.3969/j.issn.1004-1524.2020.05.06

›› 2020, Vol. 32 ›› Issue (5): 798-803.DOI: 10.3969/j.issn.1004-1524.2020.05.06

• Animal Science • Previous Articles Next Articles

Effects of microRNA-499 on lipid metabolism-related gene expression in Pelodiscus sinensis

LU Yi, GAO Youling^*, WANG Shuitao, HE Shengsheng

College of Biological and Environmental Sciences, Zhejiang Wanli University, Ningbo 315100, China

Received:2019-11-08 Online:2020-05-25 Published:2020-05-29

Abstract

Abstract: In this study, a specific inhibitor antagomirs of microRNA-499(miR-499) was injected to reveal the regulatory effect of microRNA-499 on lipid metabolism related genes of Pelodiscus sinensis. Fifteen healthy juvenile Pelodiscus sinensis were selected and randomly divided into control group, saline group and miR-499 antagomirs group, respectively. After 3 days of continuous injection at the same time, triglyceride and total cholesterol in serum and liver were determined, and the expression levels of miR-499 and lipid metabolism related genes in liver were determined by real-time quantitative PCR. The results showed that the injection of miR-499 antagomirs increased the expression levels of thyroid hormone response protein gene(THRSP) and sodium/iodine co-transporter gene(NIS), and decreased the expression level of carnitine palmitoyl transferase 1 gene(CPT1) compared with the control group and the saline group (P<0.05). The injection of miR-499 antagomirs affected the expression level of lipid metabolism genes, and miR-499 was related to fat production and fatty acid β-oxidation of Pelodiscus sinensis.

Key words: Pelodiscus sinensis, miRNA-499, antagomirs, lipid metabolism, gene expression

CLC Number:

S917.4

LU Yi, GAO Youling, WANG Shuitao, HE Shengsheng. Effects of microRNA-499 on lipid metabolism-related gene expression in Pelodiscus sinensis[J]. , 2020, 32(5): 798-803.

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Effects of microRNA-499 on lipid metabolism-related gene expression in Pelodiscus sinensis

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