Study on genetic diversity,population structure and genetic differentiation of tea germplasm in Guizhou

doi:10.3969/j.issn.1004-1524.2021.07.09

Acta Agriculturae Zhejiangensis ›› 2021, Vol. 33 ›› Issue (7): 1234-1243.DOI: 10.3969/j.issn.1004-1524.2021.07.09

• Horticultural Science • Previous Articles Next Articles

Study on genetic diversity,population structure and genetic differentiation of tea germplasm in Guizhou

AN Hongwei(), SONG Qinfei, NIU Suzhen^*()

College of Tea, Guizhou University, Guiyang 550025, China

Received:2021-03-05 Online:2021-07-25 Published:2021-08-06
Contact: NIU Suzhen

Abstract

Abstract:

In this study,68 EST-SSR primers were used to analyze the genetic diversity, population structure and genetic differentiation of 144 tea germplasm resources, which were divided into three groups by different altitude ranges and species: Gro1 represented the tea population of Camellia tachangensis(F.C.Zhang) and over 1 500 m altitude, Gro2 represented the tea population of Camellia sinensis(L.) O. Ktze and altitude from 1 000 to 1 300 m, and Gro3 represented a population whose altitude and species are not obvious. The tea germplasmresources were analyzed according to species, zones and altitude ranges, Gst was 0.079 7, 0.067 7, 0.047 0, Nm was 5.774 1, 6.885 2, 10.134 8, respectively, which showed that genetic variation existed among different individuals within populations, and there were strong gene communication among different populations.The greater the altitude gap was,the farther the genetic distance was.This study further explained the altitude effect on the tea germplasm distribution in Guizhou,laid the foundation for further study on the evolution and distribution law of tea germplasm resources in Guizhou.

Key words: tea germplasm, population structure, genetic diversity

CLC Number:

S571.1

AN Hongwei, SONG Qinfei, NIU Suzhen. Study on genetic diversity,population structure and genetic differentiation of tea germplasm in Guizhou[J]. Acta Agriculturae Zhejiangensis, 2021, 33(7): 1234-1243.

Figures/Tables 11

References 30

[1]	WAMBULWA M C, MEEGAHAKUMBURA M K, KAMUNYA S, et al. Insights into the genetic relationships and breeding patterns of the African tea germplasm based on nSSR markers and cpDNA sequences[J]. Frontiers in Plant Science, 2016, 7:1244.
[2]	LIU S R, LIU H W, WU A L, et al. Construction of fingerprinting for tea plant (Camellia sinensis) accessions using new genomic SSR markers[J]. Molecular Breeding, 2017, 37(8):1-14. DOI URL
[3]	XIA E H, ZHANG H B, SHENG J, et al. The tea tree genome provides insights into tea flavor and independent evolution of caffeine biosynjournal[J]. Molecular Plant, 2017, 10(6):866-877. DOI URL
[4]	WEI C L, YANG H, WANG S B, et al. Draft genome sequence of Camellia sinensis var. sinensis provides insights into the evolution of the tea genome and tea quality[J]. Proceedings of the National Academy of Sciences of the United States of America, 2018, 115(18):E4151-E4158.
[5]	NIU S Z, KOIWA H, SONG Q F, et al. Development of core-collections for Guizhou tea genetic resources and GWAS of leaf size using SNP developed by genotyping-by-sequencing[J]. PeerJ, 2020, 8:e8572. DOI URL
[6]	陈正武, 刘红梅, 曹雨. 贵州野生茶树资源及地方品种变异类型的保护与利用[J]. 贵州农业科学, 2009, 37(7):188-190.
	CHEN Z W, LIU H M, CAO Y. Protection and utilization of variation type of wild tea resources and local tea varieties in Guizhou[J]. Guizhou Agricultural Sciences, 2009, 37(7):188-190.(in Chinese with English abstract)
[7]	NIU S Z, SONG Q F, KOIWA H, et al. Genetic diversity, linkage disequilibrium, and population structure analysis of the tea plant (Camellia sinensis) from an origin center, Guizhou plateau, using genome-wide SNPs developed by genotyping-by-sequencing[J]. BMC Plant Biology, 2019, 19(1):328. DOI URL
[8]	陈亮. 茶组植物的分子系统学研究: 遗传多样性与分类[D]. 杭州: 浙江大学, 2002.
	CHEN L. Studies on molecular systematics of Sect.Thea(L.)Dyer[D]. Hangzhou: Zhejiang University, 2002. (in Chinese with English abstract)
[9]	刘本英. EST-SSR和ISSR分子标记在云南茶树资源中的应用研究[D]. 北京: 中国农业科学院, 2009.
	LIU B Y. Application studies of EST-SSR and ISSR markers in tea germplasms (Camellia spp.) from Yunnan[D]. Beijing: Chinese Academy of Agricultural Sciences, 2009. (in Chinese with English abstract)
[10]	乔婷婷. 茶树资源遗传多样性及其表型性状关联EST-SSR位点的初步鉴定[D]. 北京: 中国农业科学院, 2010.
	QIAO T T. Genetic diversity of tea (Camellia sinensis(l.) O. kuntze) and association analysis of phenotypic traits with EST-SSR markers[D]. Beijing: Chinese Academy of Agricultural Sciences, 2010. (in Chinese with English abstract)
[11]	姚明哲, 刘振, 陈亮, 等. 利用EST-SSR分析江北茶区茶树资源的遗传多样性和遗传结构[J]. 茶叶科学, 2009, 29(3):243-250.
	YAO M Z, LIU Z, CHEN L, et al. Genetic diversity and structure of tea germplasm originated from region of north Yangtze River based on EST-SSR markers[J]. Journal of Tea Science, 2009, 29(3):243-250.(in Chinese with English abstract)
[12]	刘本英, 李友勇, 唐一春, 等. 云南茶树资源遗传多样性与亲缘关系的ISSR分析[J]. 作物学报, 2010, 36(3):391-400.
	LIU B Y, LI Y Y, TANG Y C, et al. Genetic diversity and relationship of tea germplasm in Yunnan revealed by ISSR analysis[J]. Acta Agronomica Sinica, 2010, 36(3):391-400.(in Chinese with English abstract)
[13]	EVANNO G, REGNAUT S, GOUDET J. Detecting the number of clusters of individuals using the software structure: a simulation study[J]. Molecular Ecology, 2005, 14(8):2611-2620. DOI URL
[14]	BOTSTEIN D, WHITE R L, SKOLNICK M, et al. Construction of a genetic linkage map in man using restriction fragment length polymorphisms[J]. American Journal of Human Genetics, 1980, 32(3):314-331.
[15]	王松琳, 马春雷, 黄丹娟, 等. 基于SSR标记的白化和黄化茶树品种遗传多样性分析及指纹图谱构建[J]. 茶叶科学, 2018, 38(1):58-68.
	WANG S L, MA C L, HUANG D J, et al. Analysis of genetic diversity and construction of DNA fingerprints of chlorophyll-deficient tea cultivars by SSR markers[J]. Journal of Tea Science, 2018, 38(1):58-68.(in Chinese with English abstract)
[16]	张亚利, 宋垚, 奉树成. SSR分子标记在山茶属观赏资源遗传多样性研究中的应用[J]. 植物科学学报, 2016, 34(5):755-764.
	ZHANG Y L, SONG Y, FENG S C. Application of SSR for the analysis of genetic diversity in Camellia[J]. Plant Science Journal, 2016, 34(5):755-764.(in Chinese with English abstract)
[17]	苏会, 刘建军, 贺巍, 等. 豫南茶区茶品质相关性状与EST-SSR标记的关联分析[J]. 农业生物技术学报, 2016, 24(9):1328-1336.
	SU H, LIU J J, HE W, et al. Association analysis of traits related to tea(Camellia sinensis) quality with EST-SSRs in southern Henan area[J]. Journal of Agricultural Biotechnology, 2016, 24(9):1328-1336.(in Chinese with English abstract)
[18]	张明泽, 姚玉仙, 陈世军. 黔南60份茶树种质资源遗传多样性的SSR分析[J]. 西北植物学报, 2016, 36(6):1117-1124.
	ZHANG M Z, YAO Y X, CHEN S J. Genetic diversity analysis of tea germplasm in Qiannan prefecture by SSR markers[J]. Acta Botanica Boreali-Occidentalia Sinica, 2016, 36(6):1117-1124.(in Chinese with English abstract)
[19]	刘振, 王新超, 赵丽萍, 等. 基于EST-SSR的西南茶区茶树资源遗传多样性和亲缘关系分析[J]. 分子植物育种, 2008, 6(1):100-110.
	LIU Z, WANG X C, ZHAO L P, et al. Genetic diversity and relationship analysis of tea germplasms originated from south Western China based on EST-SSR[J]. Molecular Plant Breeding, 2008, 6(1):100-110.(in Chinese with English abstract)
[20]	孙雪梅, 赵才美, 李友勇, 等. 云南茶树地方品种与野生茶遗传多样性的ISSR分析[J]. 西南农业学报, 2018, 31(5):901-906.
	SUN X M, ZHAO C M, LI Y Y, et al. Genetic diversity of tea landraces and wild tea germplasms in Yunnan revealed by ISSR analysis[J]. Southwest China Journal of Agricultural Sciences, 2018, 31(5):901-906.(in Chinese with English abstract)
[21]	陈亮, 虞富莲, 童启庆. 关于茶组植物分类与演化的讨论[J]. 茶叶科学, 2000, 20(2):89-94.
	CHEN L, YU F L, TONG Q Q. Discussions on phylogenetic classification and evolution of sect Thea[J]. Journal of Tea Science, 2000, 20(2):89-94.(in Chinese with English abstract)
[22]	葛立雯, 郭维, 潘正康, 等. 贵州姑菁野生茶树形态多样性及相关性研究[J]. 植物遗传资源学报, 2015, 16(3):497-502.
	GE L W, GUO W, PAN Z K, et al. The morphological diversity and correlation research of gu jing wild tea plant from Guizhou[J]. Journal of Plant Genetic Resources, 2015, 16(3):497-502.(in Chinese with English abstract)
[23]	杨亚军. 中国茶树栽培学[M]. 上海: 上海科学技术出版社, 2005.
[24]	中国农业科学院茶叶研究所. 中国茶树栽培学[M]. 上海: 上海科学技术出版社, 1986.
[25]	刘声传, 曹雨, 鄢东海, 等. 贵州野生茶树资源地理分布和形态特征与气候要素的关系[J]. 茶叶科学, 2013, 33(6):517-525.
	LIU S C, CAO Y, YAN D H, et al. Geographical distribution and morphology of wild tea germplasm resources in Guizhou and its relationship with climatic factors[J]. Journal of Tea Science, 2013, 33(6):517-525.(in Chinese with English abstract)
[26]	NEI M. Estimation of average heterozygosity and genetic distance from a small number of individuals[J]. Genetics, 1978, 89(3):583-590. DOI URL
[27]	CURNOW R N, WRIGHT S. Evolution and the genetics of populations, volume 4: variability within and among natural populations[J]. Biometrics, 1979, 35(1):359. DOI URL
[28]	YANG J L, ZHAO Y J, HUANG G F, et al. Genetic diversity analysis of ancient tea plants in Xishuangbanna area[J]. Botanical Research, 2018, 7(4):442-452. DOI URL
[29]	刘振, 成杨, 杨培迪, 等. 基于nSSR和cpDNA序列的城步峒茶群体遗传多样性和结构研究[J]. 茶叶科学, 2020, 40(2):250-258.
	LIU Z, CHENG Y, YANG P D, et al. Genetic diversity and structure of chengbudong tea population revealed by nSSR and cpDNA markers[J]. Journal of Tea Science, 2020, 40(2):250-258.(in Chinese with English abstract)
[30]	李慧. 南江大叶茶种质资源形态、生化及遗传多样性的分析[D]. 雅安: 四川农业大学, 2017.
	LI H. Analysis on the germ-plasm resources’s morphology, biochemistry and genetic diversity of Nanjiang large-leaf tea[D]. Ya’an: Sichuan Agricultural University, 2017. (in Chinese with English abstract)

编号 No.	海拔 Altitude/m	种 Species	地点 Zone	推断群体 Inferential group	编号 No.	海拔 Altitude/m	种 Species	地点 Zone	推断群体 Inferential group
1	1742	大厂茶C.tachangensis	兴义Xingyi	群体1 Group 1	73	1276	茶C.sinensis	普定Puding	群体2 Group 2
2	1742	大厂茶C.tachangensis	兴义Xingyi	群体1 Group 1	74	1027	茶C.sinensis	都匀Duyun	群体2 Group 2
3	1734	大厂茶C.tachangensis	兴义Xingyi	群体1 Group 1	75	1027	茶C.sinensis	都匀Duyun	群体2 Group 2
4	1742	大厂茶C.tachangensis	兴义Xingyi	群体3 Group 3	76	1028	茶C.sinensis	都匀Duyun	群体2 Group 2
5	1744	大厂茶C.tachangensis	兴义Xingyi	群体3 Group 3	77	1029	茶C.sinensis	都匀Duyun	群体2 Group 2
6	1737	大厂茶C.tachangensis	兴义Xingyi	群体3 Group 3	78	1278	茶C.sinensis	雷山Leishan	群体2 Group 2
7	1897	大厂茶C.tachangensis	兴义Xingyi	群体3 Group 3	79	1367	茶C.sinensis	雷山Leishan	群体2 Group 2
8	1446	大厂茶C.tachangensis	贵定Guiding	群体1 Group 1	80	1867	大厂茶C.tachangensis	兴义Xingyi	群体3 Group 3
9	1744	大厂茶C.tachangensis	兴义Xingyi	群体3 Group 3	81	613	茶C.sinensis	黎平Liping	群体2 Group 2
10	1735	大厂茶C.tachangensis	兴义Xingyi	群体1 Group 1	82	613	茶C.sinensis	黎平Liping	群体2 Group 2
11	1733	大厂茶C.tachangensis	兴义Xingyi	群体1 Group 1	83	614	茶C.sinensis	黎平Liping	群体2 Group 2
12	1745	大厂茶C.tachangensis	兴义Xingyi	群体3 Group 3	84	624	茶C.sinensis	黎平Liping	群体2 Group 2
13	1738	大厂茶C.tachangensis	兴义Xingyi	群体1 Group 1	85	624	茶C.sinensis	黎平Liping	群体2 Group 2
14	1892	大厂茶C.tachangensis	兴义Xingyi	群体1 Group 1	86	628	茶C.sinensis	德江Dejiang	群体2 Group 2
15	1900	大厂茶C.tachangensis	兴义Xingyi	群体1 Group 1	87	626	茶C.sinensis	德江Dejiang	群体2 Group 2
16	1897	大厂茶C.tachangensis	兴义Xingyi	群体1 Group 1	88	1373	大厂茶C.tachangensis	贞丰Zhenfeng	群体3 Group 3
17	1742	大厂茶C.tachangensis	兴义Xingyi	群体3 Group 3	89	1712	大厂茶C.tachangensis	兴仁Xingren	群体3 Group 3
18	1744	大厂茶C.tachangensis	兴义Xingyi	群体2 Group 2	90	1849	大厂茶C.tachangensis	普安Puan	群体1 Group 1
19	1732	大厂茶C.tachangensis	兴义Xingyi	群体3 Group 3	91	1855	大厂茶C.tachangensis	普安Puan	群体1 Group 1
20	1373	大厂茶C.tachangensis	贞丰Zhenfeng	群体3 Group 3	92	1855	大厂茶C.tachangensis	普安Puan	群体1 Group 1
21	1373	大理茶C.taliensis	贞丰Zhenfeng	群体2 Group 2	93	1856	大厂茶C.tachangensis	普安Puan	群体1 Group 1
22	1364	大理茶C.taliensis	贞丰Zhenfeng	群体3 Group 3	94	1023	茶C.sinensis	印江Yinjiang	群体3 Group 3
23	1370	大理茶C.taliensis	贞丰Zhenfeng	群体3 Group 3	95	1446	大厂茶C.tachangensis	贵定Guiding	群体3 Group 3
24	1370	大理茶C.taliensis	贞丰Zhenfeng	群体3 Group 3	96	1446	大厂茶C.tachangensis	贵定Guiding	群体3 Group 3
25	1370	大理茶C.taliensis	贞丰Zhenfeng	群体3 Group 3	97	1307	茶C.sinensis	安顺Anshun	群体3 Group 3
26	621	茶C.sinensis	德江Dejiang	群体2 Group 2	98	1305	茶C.sinensis	安顺Anshun	群体2 Group 2
27	1023	茶C.sinensis	印江Yinjiang	群体2 Group 2	99	1303	茶C.sinensis	安顺Anshun	群体2 Group 2
28	1023	茶C.sinensis	印江Yinjiang	群体2 Group 2	100	1301	茶C.sinensis	安顺Anshun	群体2 Group 2
29	1072	茶C.sinensis	印江Yinjiang	群体2 Group 2	101	1108	秃房茶C.gymnogyna	务川Wuchuan	群体3 Group 3
30	1072	茶C.sinensis	印江Yinjiang	群体2 Group 2	102	1109	秃房茶C.gymnogyna	务川Wuchuan	群体1 Group 1
31	1072	茶C.sinensis	印江Yinjiang	群体2 Group 2	103	1108	秃房茶C.gymnogyna	务川Wuchuan	群体1 Group 1
32	1154	茶C.sinensis	贵阳Guiyang	群体3 Group 3	104	1108	秃房茶C.gymnogyna	务川Wuchuan	群体1 Group 1
编号 No.	海拔 Altitude/m	种 Species	地点 Zone	推断群体 Inferential group	编号 No.	海拔 Altitude/m	种 Species	地点 Zone	推断群体 Inferential group
33	1302	大理茶C.taliensis	贵阳Guiyang	群体2 Group 2	105	1108	秃房茶C.gymnogyna	务川Wuchuan	群体3 Group 3
34	1016	大理茶C.taliensis	金沙Jinsha	群体1 Group 1	106	876	秃房茶C.gymnogyna	务川Wuchuan	群体3 Group 3
35	1016	大理茶C.taliensis	金沙Jinsha	群体1 Group 1	107	877	秃房茶C.gymnogyna	务川Wuchuan	群体1 Group 1
36	974	大厂茶C.tachangensis	金沙Jinsha	群体3 Group 3	108	878	秃房茶C. gymnogyna	务川Wuchuan	群体1 Group 1
37	1290	茶C.sinensis	雷山Leishan	群体2 Group 2	109	1239	秃房茶C.gymnogyna	道真Daozhen	群体3 Group 3
38	1279	茶C.sinensis	雷山Leishan	群体2 Group 2	110	1755	大厂茶C.tachangensis	普安Puan	群体1 Group 1
39	1346	大厂茶C.tachangensis	贞丰Zhenfen	群体3 Group 3	111	1224	茶C.sinensis	道真Daozhen	群体2 Group 2
40	1478	茶C.sinensis	雷山Leishan	群体2 Group 2	112	829	茶C.sinensis	务川Wuchuan	群体2 Group 2
41	1367	茶C.sinensis	雷山Leishan	群体2 Group 2	113	1027	茶C.sinensis	都匀Duyun	群体3 Group 3
42	1150	茶C.sinensis	雷山Leishan	群体3 Group 3	114	1028	茶C.sinensis	都匀Duyun	群体3 Group 3
43	1302	茶C.sinensis	雷山Leishan	群体2 Group 2	115	772	茶C.sinensis	三都Sandu	群体2 Group 2
44	1446	大厂茶C.tachangensis	贵定Guiding	群体1 Group 1	116	878	茶C.sinensis	正安Zhengan	群体2 Group 2
45	1447	大厂茶C.tachangensis	贵定Guiding	群体1 Group 1	117	1015	大理茶C.taliensis	金沙Jinsha	群体3 Group 3
46	1448	大厂茶C.tachangensis	贵定Guiding	群体3 Group 3	118	1308	茶C.sinensis	贵阳Guiyang	群体2 Group 2
47	1449	大厂茶C.tachangensis	贵定Guiding	群体3 Group 3	119	1175	茶C.sinensis	晴隆Qinglong	群体2 Group 2
48	1712	大厂茶C.tachangensis	兴仁Xingren	群体1 Group 1	120	1175	茶C.sinensis	晴隆Qinglong	群体2 Group 2
49	1082	茶C.sinensis	贵定Guiding	群体2 Group 2	121	1616	大理茶C.taliensis	安龙Anlong	群体1 Group 1
50	1027	茶C.sinensis	都匀Duyun	群体2 Group 2	122	1175	茶C.sinensis	晴隆Qinglong	群体2 Group 2
51	1082	茶C.sinensis	贵定Guiding	群体2 Group 2	123	1176	茶C.sinensis	晴隆Qinglong	群体3 Group 3
52	1456	茶C.sinensis	贵定Guiding	群体2 Group 2	124	1177	茶C.sinensis	晴隆Qinglong	群体2 Group 2
53	1457	茶C.sinensis	贵定Guiding	群体2 Group 2	125	1178	茶C.sinensis	晴隆Qinglong	群体2 Group 2
54	1131	茶C.sinensis	务川Wuchuan	群体2 Group 2	126	1155	茶C.sinensis	贵阳Guiyang	群体2 Group 2
55	1132	茶C.sinensis	务川Wuchuan	群体2 Group 2	127	1276	茶C.sinensis	普定Puding	群体2 Group 2
56	829	茶C.sinensis	务川Wuchuan	群体2 Group 2	128	1154	茶C.sinensis	贵阳Guiyang	群体2 Group 2
57	1446	大理茶C.taliensis	贵定Guiding	群体1 Group 1	129	1178	茶C.sinensis	晴隆Qinglong	群体2 Group 2
58	853	茶C.sinensis	务川Wuchuan	群体2 Group 2	130	1175	茶C.sinensis	晴隆Qinglong	群体2 Group 2
59	949	茶C.sinensis	正安Zhengan	群体3 Group 3	131	1175	茶C.sinensis	晴隆Qinglong	群体2 Group 2
60	878	茶C.sinensis	正安Zhengan	群体2 Group 2	132	1175	茶C.sinensis	晴隆Qinglong	群体2 Group 2
61	1027	茶C.sinensis	荔波Libo	群体3 Group 3	133	1346	茶C.sinensis	普安Puan	群体2 Group 2
62	884	茶C.sinensis	三都Sandu	群体2 Group 2	134	1147	茶C.sinensis	晴隆Qinglong	群体2 Group 2
63	785	茶C.sinensis	三都Sandu	群体2 Group 2	135	1148	大厂茶C.tachangensis	晴隆Qinglong	群体2 Group 2
64	1027	茶C.sinensis	都匀Duyun	群体2 Group 2	136	1895	大厂茶C.tachangensis	普安Puan	群体1 Group 1
65	1027	茶C.sinensis	都匀uyun	群体2 Group 2	137	1175	茶C.sinensis	晴隆Qinglong	群体2 Group 2
66	1027	茶C.sinensis	都匀Duyun	群体2 Group 2	138	1148	茶C.sinensis	晴隆Qinglong	群体2 Group 2
67	1027	茶C.sinensis	都匀Duyun	群体2 Group 2	139	1148	茶C.sinensis	晴隆Qinglong	群体2 Group 2
68	1108	秃房茶C.gymnogyna	务川Wuchuan	群体3 Group 3	140	1389	茶C.sinensis	黎平Liping	群体2 Group 2
69	1131	秃房茶C.gymnogyna	务川Wuchuan	群体3 Group 3	141	1402	茶C.sinensis	黎平Liping	群体2 Group 2
70	1132	秃房茶C.gymnogyna	务川Wuchuan	群体2 Group 2	142	1356	茶C.sinensis	黎平Liping	群体2 Group 2
71	872	秃房茶C.gymnogyna	务川Wuchuan	群体2 Group 2	143	1356	茶C.sinensis	黎平Liping	群体2 Group 2
72	872	秃房茶C.gymnogyna	务川Wuchuan	群体2 Group 2	144	1356	茶C.sinensis	黎平Liping	群体3 Group 3

编号 No.	海拔 Altitude/m	种 Species	地点 Zone	推断群体 Inferential group	编号 No.	海拔 Altitude/m	种 Species	地点 Zone	推断群体 Inferential group
1	1742	大厂茶C.tachangensis	兴义Xingyi	群体1 Group 1	73	1276	茶C.sinensis	普定Puding	群体2 Group 2
2	1742	大厂茶C.tachangensis	兴义Xingyi	群体1 Group 1	74	1027	茶C.sinensis	都匀Duyun	群体2 Group 2
3	1734	大厂茶C.tachangensis	兴义Xingyi	群体1 Group 1	75	1027	茶C.sinensis	都匀Duyun	群体2 Group 2
4	1742	大厂茶C.tachangensis	兴义Xingyi	群体3 Group 3	76	1028	茶C.sinensis	都匀Duyun	群体2 Group 2
5	1744	大厂茶C.tachangensis	兴义Xingyi	群体3 Group 3	77	1029	茶C.sinensis	都匀Duyun	群体2 Group 2
6	1737	大厂茶C.tachangensis	兴义Xingyi	群体3 Group 3	78	1278	茶C.sinensis	雷山Leishan	群体2 Group 2
7	1897	大厂茶C.tachangensis	兴义Xingyi	群体3 Group 3	79	1367	茶C.sinensis	雷山Leishan	群体2 Group 2
8	1446	大厂茶C.tachangensis	贵定Guiding	群体1 Group 1	80	1867	大厂茶C.tachangensis	兴义Xingyi	群体3 Group 3
9	1744	大厂茶C.tachangensis	兴义Xingyi	群体3 Group 3	81	613	茶C.sinensis	黎平Liping	群体2 Group 2
10	1735	大厂茶C.tachangensis	兴义Xingyi	群体1 Group 1	82	613	茶C.sinensis	黎平Liping	群体2 Group 2
11	1733	大厂茶C.tachangensis	兴义Xingyi	群体1 Group 1	83	614	茶C.sinensis	黎平Liping	群体2 Group 2
12	1745	大厂茶C.tachangensis	兴义Xingyi	群体3 Group 3	84	624	茶C.sinensis	黎平Liping	群体2 Group 2
13	1738	大厂茶C.tachangensis	兴义Xingyi	群体1 Group 1	85	624	茶C.sinensis	黎平Liping	群体2 Group 2
14	1892	大厂茶C.tachangensis	兴义Xingyi	群体1 Group 1	86	628	茶C.sinensis	德江Dejiang	群体2 Group 2
15	1900	大厂茶C.tachangensis	兴义Xingyi	群体1 Group 1	87	626	茶C.sinensis	德江Dejiang	群体2 Group 2
16	1897	大厂茶C.tachangensis	兴义Xingyi	群体1 Group 1	88	1373	大厂茶C.tachangensis	贞丰Zhenfeng	群体3 Group 3
17	1742	大厂茶C.tachangensis	兴义Xingyi	群体3 Group 3	89	1712	大厂茶C.tachangensis	兴仁Xingren	群体3 Group 3
18	1744	大厂茶C.tachangensis	兴义Xingyi	群体2 Group 2	90	1849	大厂茶C.tachangensis	普安Puan	群体1 Group 1
19	1732	大厂茶C.tachangensis	兴义Xingyi	群体3 Group 3	91	1855	大厂茶C.tachangensis	普安Puan	群体1 Group 1
20	1373	大厂茶C.tachangensis	贞丰Zhenfeng	群体3 Group 3	92	1855	大厂茶C.tachangensis	普安Puan	群体1 Group 1
21	1373	大理茶C.taliensis	贞丰Zhenfeng	群体2 Group 2	93	1856	大厂茶C.tachangensis	普安Puan	群体1 Group 1
22	1364	大理茶C.taliensis	贞丰Zhenfeng	群体3 Group 3	94	1023	茶C.sinensis	印江Yinjiang	群体3 Group 3
23	1370	大理茶C.taliensis	贞丰Zhenfeng	群体3 Group 3	95	1446	大厂茶C.tachangensis	贵定Guiding	群体3 Group 3
24	1370	大理茶C.taliensis	贞丰Zhenfeng	群体3 Group 3	96	1446	大厂茶C.tachangensis	贵定Guiding	群体3 Group 3
25	1370	大理茶C.taliensis	贞丰Zhenfeng	群体3 Group 3	97	1307	茶C.sinensis	安顺Anshun	群体3 Group 3
26	621	茶C.sinensis	德江Dejiang	群体2 Group 2	98	1305	茶C.sinensis	安顺Anshun	群体2 Group 2
27	1023	茶C.sinensis	印江Yinjiang	群体2 Group 2	99	1303	茶C.sinensis	安顺Anshun	群体2 Group 2
28	1023	茶C.sinensis	印江Yinjiang	群体2 Group 2	100	1301	茶C.sinensis	安顺Anshun	群体2 Group 2
29	1072	茶C.sinensis	印江Yinjiang	群体2 Group 2	101	1108	秃房茶C.gymnogyna	务川Wuchuan	群体3 Group 3
30	1072	茶C.sinensis	印江Yinjiang	群体2 Group 2	102	1109	秃房茶C.gymnogyna	务川Wuchuan	群体1 Group 1
31	1072	茶C.sinensis	印江Yinjiang	群体2 Group 2	103	1108	秃房茶C.gymnogyna	务川Wuchuan	群体1 Group 1
32	1154	茶C.sinensis	贵阳Guiyang	群体3 Group 3	104	1108	秃房茶C.gymnogyna	务川Wuchuan	群体1 Group 1
编号 No.	海拔 Altitude/m	种 Species	地点 Zone	推断群体 Inferential group	编号 No.	海拔 Altitude/m	种 Species	地点 Zone	推断群体 Inferential group
33	1302	大理茶C.taliensis	贵阳Guiyang	群体2 Group 2	105	1108	秃房茶C.gymnogyna	务川Wuchuan	群体3 Group 3
34	1016	大理茶C.taliensis	金沙Jinsha	群体1 Group 1	106	876	秃房茶C.gymnogyna	务川Wuchuan	群体3 Group 3
35	1016	大理茶C.taliensis	金沙Jinsha	群体1 Group 1	107	877	秃房茶C.gymnogyna	务川Wuchuan	群体1 Group 1
36	974	大厂茶C.tachangensis	金沙Jinsha	群体3 Group 3	108	878	秃房茶C. gymnogyna	务川Wuchuan	群体1 Group 1
37	1290	茶C.sinensis	雷山Leishan	群体2 Group 2	109	1239	秃房茶C.gymnogyna	道真Daozhen	群体3 Group 3
38	1279	茶C.sinensis	雷山Leishan	群体2 Group 2	110	1755	大厂茶C.tachangensis	普安Puan	群体1 Group 1
39	1346	大厂茶C.tachangensis	贞丰Zhenfen	群体3 Group 3	111	1224	茶C.sinensis	道真Daozhen	群体2 Group 2
40	1478	茶C.sinensis	雷山Leishan	群体2 Group 2	112	829	茶C.sinensis	务川Wuchuan	群体2 Group 2
41	1367	茶C.sinensis	雷山Leishan	群体2 Group 2	113	1027	茶C.sinensis	都匀Duyun	群体3 Group 3
42	1150	茶C.sinensis	雷山Leishan	群体3 Group 3	114	1028	茶C.sinensis	都匀Duyun	群体3 Group 3
43	1302	茶C.sinensis	雷山Leishan	群体2 Group 2	115	772	茶C.sinensis	三都Sandu	群体2 Group 2
44	1446	大厂茶C.tachangensis	贵定Guiding	群体1 Group 1	116	878	茶C.sinensis	正安Zhengan	群体2 Group 2
45	1447	大厂茶C.tachangensis	贵定Guiding	群体1 Group 1	117	1015	大理茶C.taliensis	金沙Jinsha	群体3 Group 3
46	1448	大厂茶C.tachangensis	贵定Guiding	群体3 Group 3	118	1308	茶C.sinensis	贵阳Guiyang	群体2 Group 2
47	1449	大厂茶C.tachangensis	贵定Guiding	群体3 Group 3	119	1175	茶C.sinensis	晴隆Qinglong	群体2 Group 2
48	1712	大厂茶C.tachangensis	兴仁Xingren	群体1 Group 1	120	1175	茶C.sinensis	晴隆Qinglong	群体2 Group 2
49	1082	茶C.sinensis	贵定Guiding	群体2 Group 2	121	1616	大理茶C.taliensis	安龙Anlong	群体1 Group 1
50	1027	茶C.sinensis	都匀Duyun	群体2 Group 2	122	1175	茶C.sinensis	晴隆Qinglong	群体2 Group 2
51	1082	茶C.sinensis	贵定Guiding	群体2 Group 2	123	1176	茶C.sinensis	晴隆Qinglong	群体3 Group 3
52	1456	茶C.sinensis	贵定Guiding	群体2 Group 2	124	1177	茶C.sinensis	晴隆Qinglong	群体2 Group 2
53	1457	茶C.sinensis	贵定Guiding	群体2 Group 2	125	1178	茶C.sinensis	晴隆Qinglong	群体2 Group 2
54	1131	茶C.sinensis	务川Wuchuan	群体2 Group 2	126	1155	茶C.sinensis	贵阳Guiyang	群体2 Group 2
55	1132	茶C.sinensis	务川Wuchuan	群体2 Group 2	127	1276	茶C.sinensis	普定Puding	群体2 Group 2
56	829	茶C.sinensis	务川Wuchuan	群体2 Group 2	128	1154	茶C.sinensis	贵阳Guiyang	群体2 Group 2
57	1446	大理茶C.taliensis	贵定Guiding	群体1 Group 1	129	1178	茶C.sinensis	晴隆Qinglong	群体2 Group 2
58	853	茶C.sinensis	务川Wuchuan	群体2 Group 2	130	1175	茶C.sinensis	晴隆Qinglong	群体2 Group 2
59	949	茶C.sinensis	正安Zhengan	群体3 Group 3	131	1175	茶C.sinensis	晴隆Qinglong	群体2 Group 2
60	878	茶C.sinensis	正安Zhengan	群体2 Group 2	132	1175	茶C.sinensis	晴隆Qinglong	群体2 Group 2
61	1027	茶C.sinensis	荔波Libo	群体3 Group 3	133	1346	茶C.sinensis	普安Puan	群体2 Group 2
62	884	茶C.sinensis	三都Sandu	群体2 Group 2	134	1147	茶C.sinensis	晴隆Qinglong	群体2 Group 2
63	785	茶C.sinensis	三都Sandu	群体2 Group 2	135	1148	大厂茶C.tachangensis	晴隆Qinglong	群体2 Group 2
64	1027	茶C.sinensis	都匀Duyun	群体2 Group 2	136	1895	大厂茶C.tachangensis	普安Puan	群体1 Group 1
65	1027	茶C.sinensis	都匀uyun	群体2 Group 2	137	1175	茶C.sinensis	晴隆Qinglong	群体2 Group 2
66	1027	茶C.sinensis	都匀Duyun	群体2 Group 2	138	1148	茶C.sinensis	晴隆Qinglong	群体2 Group 2
67	1027	茶C.sinensis	都匀Duyun	群体2 Group 2	139	1148	茶C.sinensis	晴隆Qinglong	群体2 Group 2
68	1108	秃房茶C.gymnogyna	务川Wuchuan	群体3 Group 3	140	1389	茶C.sinensis	黎平Liping	群体2 Group 2
69	1131	秃房茶C.gymnogyna	务川Wuchuan	群体3 Group 3	141	1402	茶C.sinensis	黎平Liping	群体2 Group 2
70	1132	秃房茶C.gymnogyna	务川Wuchuan	群体2 Group 2	142	1356	茶C.sinensis	黎平Liping	群体2 Group 2
71	872	秃房茶C.gymnogyna	务川Wuchuan	群体2 Group 2	143	1356	茶C.sinensis	黎平Liping	群体2 Group 2
72	872	秃房茶C.gymnogyna	务川Wuchuan	群体2 Group 2	144	1356	茶C.sinensis	黎平Liping	群体3 Group 3

编号 No.	重复位点 Repeat motif	目的片段大小 Size range/bp	PIC	编号 No.	重复位点 Repeat motif	目的片段大小 Size range/bp	PIC
YIN3	(TC)15	178~197	0.576 5	YIN46	(GAGTGT)4	160~200	0.287 0
YIN7	(TC)13	120~130	0.503 5	YIN47	(TTGTG)3	95~125	0.310 9
YIN8	(TG)8(AG)10	145~155	0.591 0	YIN48	(CCT)5	145~180	0.321 9
YIN9	(CTCTT)3	240	0.437 6	YIN49	(CAT)8	230~260	0.310 3
YIN10	(GGAAA)11	160~175	0.343 8	YIN50	(TTTTG)5	220~270	0.317 7
YIN13	(GTGGA)5	260~280	0.435 2	YIN51	(AGAGA)3	195~330	0.235 5
YIN14	(CT)9	246~265	0.309 5	YIN52	(AT)11	180~205	0.259 8
YIN15	(AG)14	135~148	0.313 8	YIN54	(GCACCA)3	250~290	0.380 7
YIN17	(CT)15	258	0.478 6	YIN55	(AAAAT)3	140~175	0.310 0
YIN21	(TGGAGG)5	204	0.314 3	YIN57	(ACC)8	135~155	0.294 5
YIN22	(TCTGAA)4	110~140	0.488 7	YIN58	(TTTGC)3	120~130	0.107 7
YIN23	A27	306	0.168 2	YIN59	(TTTTTA)3	220~250	0.266 0
YIN24	(CA)12	82~160	0.579 8	YIN67	(CTCA)5	100~160	0.245 4
YIN25	(GT)13(CA)23	222	0.487 5	YIN69	(GCTA)5	160~170	0.282 0
YIN26	A11	189	0.139 3	YIN70	(AAGGTAC)3	180~250	0.275 6
YIN28	(TG)13	160~210	0.289 8	YIN71	(TTTTTG)4	160~210	0.285 5
YIN31	(AG)12	95~120	0.304 1	YIN72	(CAG)5	175~195	0.243 0
YIN32	(GA)9	105~120	0.230 2	YIN75	(TGAG)4	240~300	0.285 9
YIN33	(AG)14	115~130	0.375 3	YIN77	(TCTCTG)3	90~145	0.291 8
YIN34	(GGT)6	170~180	0.446 7	YIN78	(ACTCT)3	175~210	0.320 6
YIN35	(TCA)6	250~270	0.331 3	YIN79	(TATTT)3	185~230	0.280 7
YIN36	(CTCAAT)5	195~215	0.406 8	YIN80	(TGA)8	148~170	0.407 1
YIN37	(CT)11	105~120	0.348 7	YIN82	(TTTC)4	120~140	0.235 1
YIN90	(TAAAA)5	250~270	0.280 4	YIN38	(TGAGT)4	200~210	0.525 8
YIN83	(TTTATT)3	120~135	0.574 0	YIN39	(AT)10	245~265	0.647 6
YIN84	(CACCAA)3	280~300	0.248 6	YIN40	(TC)10	230~255	0.560 5
YIN86	(AAGAA)3	220~260	0.212 7	YIN42	(CCTCCA)7	135~145	0.303 4
YIN87	(TGATG)4	160~200	0.384 2	YIN44	(TC)9	250~270	0.301 4
YIN88	(TTTTC)5	100~135	0.280 1	YIN45	(GTG)5	175~215	0.299 4
YIN89	(GTTTTT)3	160~175	0.314 4	YIN100	(ACC)6	130~150	0.343 8
YIN91	(GCG)7	160~205	0.370 0	YIN92	(CAC)5	160~195	0.109 6
YIN94	(AGG)7	120~150	0.239 4	YIN95	(TAC)6	175~200	0.228 0
YIN96	(TTTG)3	145~175	0.222 2	YIN97	(TA)11	160~170	0.405 2
YIN98	(TA)9	100~120	0.230 8	YIN99	(GAT)9	165~200	0.562 1

编号 No.	重复位点 Repeat motif	目的片段大小 Size range/bp	PIC	编号 No.	重复位点 Repeat motif	目的片段大小 Size range/bp	PIC
YIN3	(TC)15	178~197	0.576 5	YIN46	(GAGTGT)4	160~200	0.287 0
YIN7	(TC)13	120~130	0.503 5	YIN47	(TTGTG)3	95~125	0.310 9
YIN8	(TG)8(AG)10	145~155	0.591 0	YIN48	(CCT)5	145~180	0.321 9
YIN9	(CTCTT)3	240	0.437 6	YIN49	(CAT)8	230~260	0.310 3
YIN10	(GGAAA)11	160~175	0.343 8	YIN50	(TTTTG)5	220~270	0.317 7
YIN13	(GTGGA)5	260~280	0.435 2	YIN51	(AGAGA)3	195~330	0.235 5
YIN14	(CT)9	246~265	0.309 5	YIN52	(AT)11	180~205	0.259 8
YIN15	(AG)14	135~148	0.313 8	YIN54	(GCACCA)3	250~290	0.380 7
YIN17	(CT)15	258	0.478 6	YIN55	(AAAAT)3	140~175	0.310 0
YIN21	(TGGAGG)5	204	0.314 3	YIN57	(ACC)8	135~155	0.294 5
YIN22	(TCTGAA)4	110~140	0.488 7	YIN58	(TTTGC)3	120~130	0.107 7
YIN23	A27	306	0.168 2	YIN59	(TTTTTA)3	220~250	0.266 0
YIN24	(CA)12	82~160	0.579 8	YIN67	(CTCA)5	100~160	0.245 4
YIN25	(GT)13(CA)23	222	0.487 5	YIN69	(GCTA)5	160~170	0.282 0
YIN26	A11	189	0.139 3	YIN70	(AAGGTAC)3	180~250	0.275 6
YIN28	(TG)13	160~210	0.289 8	YIN71	(TTTTTG)4	160~210	0.285 5
YIN31	(AG)12	95~120	0.304 1	YIN72	(CAG)5	175~195	0.243 0
YIN32	(GA)9	105~120	0.230 2	YIN75	(TGAG)4	240~300	0.285 9
YIN33	(AG)14	115~130	0.375 3	YIN77	(TCTCTG)3	90~145	0.291 8
YIN34	(GGT)6	170~180	0.446 7	YIN78	(ACTCT)3	175~210	0.320 6
YIN35	(TCA)6	250~270	0.331 3	YIN79	(TATTT)3	185~230	0.280 7
YIN36	(CTCAAT)5	195~215	0.406 8	YIN80	(TGA)8	148~170	0.407 1
YIN37	(CT)11	105~120	0.348 7	YIN82	(TTTC)4	120~140	0.235 1
YIN90	(TAAAA)5	250~270	0.280 4	YIN38	(TGAGT)4	200~210	0.525 8
YIN83	(TTTATT)3	120~135	0.574 0	YIN39	(AT)10	245~265	0.647 6
YIN84	(CACCAA)3	280~300	0.248 6	YIN40	(TC)10	230~255	0.560 5
YIN86	(AAGAA)3	220~260	0.212 7	YIN42	(CCTCCA)7	135~145	0.303 4
YIN87	(TGATG)4	160~200	0.384 2	YIN44	(TC)9	250~270	0.301 4
YIN88	(TTTTC)5	100~135	0.280 1	YIN45	(GTG)5	175~215	0.299 4
YIN89	(GTTTTT)3	160~175	0.314 4	YIN100	(ACC)6	130~150	0.343 8
YIN91	(GCG)7	160~205	0.370 0	YIN92	(CAC)5	160~195	0.109 6
YIN94	(AGG)7	120~150	0.239 4	YIN95	(TAC)6	175~200	0.228 0
YIN96	(TTTG)3	145~175	0.222 2	YIN97	(TA)11	160~170	0.405 2
YIN98	(TA)9	100~120	0.230 8	YIN99	(GAT)9	165~200	0.562 1

	居群 Populations	样品数 Sample size	多态性位点百分率 Percentage of polymorphic loci/%	Ne	H	I
种Species	秃房茶 C.gymnogyna	14	71.82	1.291 5	0.187 0	0.297 8
	茶 C.sinensis	80	91.99	1.279 1	0.185 5	0.304 7
	大厂茶C.tachangensis	39	87.57	1.275 8	0.184 8	0.304 1
	疑似大理茶C.taliensis(W.W.Smith)	11	68.51	1.275 5	0.177 4	0.283 0
地区Zone	黔中 Qianzhong	11	61.88	1.247 0	0.158 7	0.253 4
	黔北 Qianbei	36	87.85	1.294 1	0.193 3	0.314 8
	黔南 Qiannan	27	85.08	1.282 5	0.186 9	0.305 3
	黔东南 Qiandongnan	18	73.76	1.270 3	0.175 1	0.282 4
	黔西南 Qianxinan	52	89.78	1.278 5	0.188 3	0.310 4
海拔范围	500~1 000 m	23	77.90	1.280 6	0.182 4	0.294 3
Altitude/m	1 000~1 300 m	59	93.09	1.286 2	0.191 4	0.315 2
	1 300~1 500 m	34	87.57	1.285 7	0.191 7	0.314 0
	1 500 m以上	28	78.18	1.263 3	0.173 8	0.283 6

Study on genetic diversity,population structure and genetic differentiation of tea germplasm in Guizhou

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种Species	秃房茶C.gymnogyna	茶C.sinensis	大厂茶C.tachangensis
秃房茶C.gymnogyna
茶C.sinensis	0.027 3
大厂茶C.tachangensis	0.026 4	0.017 8
疑似大理茶C.taliensis(W.W.Smith)	0.038 9	0.025 1	0.022 6

地区Zone	黔中Qianzhong	黔北Qianbei	黔南Qiannan	黔东南Qiandongnan
黔中Qianzhong
黔北Qianbei	0.026 5
黔南Qiannan	0.032 2	0.007 8
黔东南Qiandongnan	0.031 5	0.015 2	0.014 2
黔西南Qianxinan	0.032 4	0.010 7	0.011 3	0.019 0

海拔范围 Altitude/m	500~ 1 000 m	1 000~ 1 300 m	1 300~ 1 500 m
500~1 000 m
1 000~1 300 m	0.008 3
1 300~1 500 m	0.012 6	0.006 6
1 500 m以上	0.025 6	0.020 4	0.016 4
More than 1 500 m

项目	样本 Sample	Ht	Hs	Gst	Nm
种Species	144	0.199 6	0.183 7	0.079 7	5.774 1
地区Zone	144	0.193 6	0.180 5	0.067 7	6.885 2
海拔Altitude	144	0.193 9	0.184 8	0.047 0	10.134 8

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[2]	ZHAI Xiuming, LI Jie, TANG Min, HU Fangjie, ZHANG Jun, HOU Yujia, XU Ze. Diversity analysis of 30 tea (Camelia sinensis) germplasm resources in Chongqing based on agronomic traits and biochemical components [J]. Acta Agriculturae Zhejiangensis, 2021, 33(7): 1244-1255.
[3]	HONG Xia, ZHAO Yongbin, QU Weidong, CHEN Yinlong, QIU Liping, WANG Jiaoyang. Comparative analysis on genetic diversity of Colocasia esculenta germplasm in Zhejiang Province based on phenotype and simple sequence repeats markers [J]. , 2020, 32(9): 1544-1554.
[4]	NIU Suzhen, AN Hongwei, SONG Qinfei, CHEN Zhengwu. Analysis and evaluation of soil nutrients of wild tea germplasm in Guizhou [J]. , 2020, 32(6): 1039-1048.
[5]	YAN Fulin, WANG Bo, WEN Di, XU Wenfen, SUN Qingwen, WEI Shenghua. Genetic diversity analysis of Sabia parviflora based on chloroplast gene psbA-trnH sequence [J]. , 2020, 32(5): 810-815.
[6]	GUO Qinwei, ZHANG Ting, LIU Huiqin, ZHANG Xinhui, LI Chaosen, XIANG Xiaomin, ZHAO Dongfeng, WAN Hongjian. Evaluation of genetic diversity in Luffa germplasm resources in China based on ISSR molecular marker [J]. , 2020, 32(4): 616-623.
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[8]	HU Qianwen, XU Yanhao, WANG Rong, ZHANG Wenying, HUA Wei, LYU Chao. Association analysis of four spike traits in barley [J]. , 2020, 32(11): 1941-1953.
[9]	YIN Xiaoxiao, LI Yanfang, GU Jiang, LIAO Yan, XIE Yue, YANG Guangyou, GU Xiaobin. Genetic diversity analysis of Psoroptes ovis var. cuniculi by full-length of mitochondrial ATP6 gene in China [J]. , 2019, 31(8): 1231-1238.
[10]	TANG Shoujie, BI Xiang, ZHANG Feiming, ZHANG Youliang. Genetic diversity analysis and RAPD identification of three successive generations of meio-gynogenetic population in Megalobrama amblycephala [J]. , 2019, 31(8): 1257-1271.
[11]	YUE Gaohong, PAN Binrong, LIU Yongan, MEI Xixue, XU Likui, ZHANG Zongchen, ZHOU Zhihui. Genetic diversity analysis of sweet maize inbred lines in Southern Zhejiang by SSR markers [J]. , 2019, 31(7): 1029-1036.
[12]	JIANG Wu, WU Shiwu, WU Zhigang, TAO Zhengming. Genetic diversity analysis of Gardenia jasminoides in Zhejiang based on SRAP molecular marker technique [J]. , 2019, 31(3): 408-413.
[13]	WANG Weike, YUAN Weidong, LU Na, SONG Jiling, YAN Jing. Genetic diversity of wild Sanghuangporus sanghuang based on ISSR molecular markers [J]. , 2019, 31(3): 414-419.
[14]	ZHAO Wenjie, XU Wei, ZHANG Jinglong, CHENG Shuang, ZHENG Jiacheng, LIU Yanlong, YIN Shixia, LI Jieqin, ZHAN Qiuwen. Genetic diversity of sweet sorghum as revealed by biological characters and SSR markers [J]. , 2019, 31(12): 1945-1954.
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