Protective effects of Glycyrrhiza total flavones on liver injury of tilapia (Oreochromis niloticus) under high fat condition

doi:10.3969/j.issn.1004-1524.2021.10.05

Acta Agriculturae Zhejiangensis ›› 2021, Vol. 33 ›› Issue (10): 1826-1835.DOI: 10.3969/j.issn.1004-1524.2021.10.05

• Animal Science • Previous Articles Next Articles

Protective effects of Glycyrrhiza total flavones on liver injury of tilapia (Oreochromis niloticus) under high fat condition

DU Jinliang¹^,²^,³(), CAO Liping¹^,², JIA Rui¹^,², GU Zhengyan⁴, HE Qin⁴, XU Pao¹^,²^,⁴, JENEY Galina²^,⁵, MA Yuzhong³^,^*(), YIN Guojun¹^,²^,⁴^,^*()

1. Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture and Rural Affairs/Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China
2. International Joint Research Laboratory for Fish Immunopharmacology/Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China
3. College of Veterinary Medicine, Hebei Agricultural University, Baoding 071001, China
4. Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China
5. National Agricultural Research Center, Research Institute for Fisheries and Aquaculture, Szarvas 5440, Hungary

Received:2020-11-18 Online:2021-10-25 Published:2021-11-02
Contact: MA Yuzhong,YIN Guojun

Abstract

Abstract:

In order to evaluate protective effects of Glycyrrhiza total flavones (GTF) on liver of tilapia (Oreochromis niloticus) after fatty liver injury, 180 healthy and uninjured tilapia were randomly divided into six experimental groups. Namely, control group (CK), high-fat diet (HFD) model group and four different concentrations (0.05, 0.10, 0.50, 1.00 g·kg ^-1) of Glycyrrhiza total flavones treatment groups, each group 30 tails. After 90 days of feeding, liver were collected for determination activities of superoxide dismutase (SOD), reduced glutathione (GSH), contents of malondialdehyde (MDA), triglyceride (TG) and total cholesterol (TC), and level of total antioxidant capacity (T-AOC) in liver homogenate. Western blotting method was used to measure the protein levels of nuclear transcription factor-κB C-rel (NF-κB C-Rel) and nuclear transcription factor-κB P 65 (NF-κB P65) in liver. Quantitative real-time PCR method was used to measure mRNA levels of microsomal triglyceride transfer protein (MTTP) and apolipoprotein B100 (ApoB100). The results showed that: compared with the control group, contents of TG, TC and MDA in tilapia liver homogenate in high-fat diet group significantly increased (P< 0.01), activities of GSH, SOD and T-AOC level in liver homogenate significantly decreased (P<0.01), and mRNA levels of MTTP, ApoB100 down-regulated (P<0.01 or P<0.05), NF-κB P65 and C-rel protein levels increased. After adding Glycyrrhiza total flavones to the diet, activities of GSH, SOD and T-AOC level in liver were increased in different degrees, contents of TC, TG, MDA, and NF-κB C-Rel, NF-κB P65 protein levels were decreased in different degrees, mRNA levels of MTTP and ApoB100 showed an up-regulated trend, and concentration of Glycyrrhiza total flavones at 0.50 g·kg ^-1 showed the best effects. Overall, these results indicated that Glycyrrhiza total flavones had certain effects on tilapia fatty liver and could alleviate the fatty liver injury caused by high-fat diet.

Key words: Glycyrrhiza uralensis Fisch, total flavonoids, tilapia, fatty liver, gene expression, western blotting

CLC Number:

S965.125

DU Jinliang, CAO Liping, JIA Rui, GU Zhengyan, HE Qin, XU Pao, JENEY Galina, MA Yuzhong, YIN Guojun. Protective effects of Glycyrrhiza total flavones on liver injury of tilapia (Oreochromis niloticus) under high fat condition[J]. Acta Agriculturae Zhejiangensis, 2021, 33(10): 1826-1835.

Figures/Tables 9

References 43

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基因 Genes	引物序列 Primer sequence (5'-3')	Genbank登录号 GenBank accession No.
MTTP	F: TGCATGTGGCTTTGCTTCAC R: CTTGACCTGCTCCACGGTAG	XM_005458713.4
ApoB100	F: ACTAGAAGGCAGACCAACCA R: CCTGCCATTTCAACACCCATT	XM_003446376.4
β-actin	F: CCTGAGCGTAAATACTCCGTCTG R: AAGCACTTGCGGTGGACGAT	KJ126772.1

基因 Genes	引物序列 Primer sequence (5'-3')	Genbank登录号 GenBank accession No.
MTTP	F: TGCATGTGGCTTTGCTTCAC R: CTTGACCTGCTCCACGGTAG	XM_005458713.4
ApoB100	F: ACTAGAAGGCAGACCAACCA R: CCTGCCATTTCAACACCCATT	XM_003446376.4
β-actin	F: CCTGAGCGTAAATACTCCGTCTG R: AAGCACTTGCGGTGGACGAT	KJ126772.1

Protective effects of Glycyrrhiza total flavones on liver injury of tilapia (Oreochromis niloticus) under high fat condition

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成分 Ingredients/ (g·kg^-1)		鱼粉 Fish meal (CP 53.5%)/ (g·kg^-1)	酪蛋白 Casein/ (g·kg^-1)	明胶 Gelatin/ (g·kg^-1)	玉米淀粉 Corn starch/ (g·kg^-1)	维生素预混料¹⁾ Vitamin premix/ (g·kg^-1)	矿物质预混料²⁾ Mineral premix/ (g·kg^-1)	纤维素 Cellulose/ (g·kg^-1)	羧甲基纤维素钠 Carboxymethylc- ellulose sodium/ (g·kg^-1)
对照组Control group		90	200	70	440.00	10	20	80	30
高脂组High-fat-fed group		90	200	70	282.00	10	20	80	30
不同浓度(g·kg^-1)	0.05	90	200	70	281.95	10	20	80	30
甘草总黄酮组	0.10	90	200	70	281.90	10	20	80	30
Different concentrations	0.50	90	200	70	281.50	10	20	80	30
(g·kg^-1) of Glycyrrhiza	1.00	90	200	70	281.00	10	20	80	30
total flavones groups
成分 Ingredients/ (g·kg^-1)		氯化胆碱 Choline chloride/ (g·kg^-1)	猪油 Lard/ (g·kg^-1)	胆固醇 Choleste- rol/(g· kg^-1)	大豆油 Soybean oil/(g· kg^-1)	甘草总黄酮 GTF/ (g· kg^-1)	粗蛋白质 Crude protein/ %	粗脂肪 Crude lipid/%	灰分 Ash/%
对照组Control group		5	0	0	55	0	28	6.0	2.7
高脂组High-fat-fed group		5	135	5	73	0	28	21.1	2.8
不同浓度(g·kg^-1)	0.05	5	135	5	73	0.05	28	21.1	2.8
甘草总黄酮组	0.10	5	135	5	73	0.10	28	21.1	2.8
Different concentrations	0.50	5	135	5	73	0.50	28	21.1	2.8
(g·kg^-1) of Glycyrrhiza	1.00	5	135	5	73	1.00	28	21.1	2.8
total flavones groups

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