Expression analysis of bZIP G subfamily genes in maize

doi:10.3969/j.issn.1004-1524.2022.02.02

Acta Agriculturae Zhejiangensis ›› 2022, Vol. 34 ›› Issue (2): 221-231.DOI: 10.3969/j.issn.1004-1524.2022.02.02

• Crop Science • Previous Articles Next Articles

Expression analysis of bZIP G subfamily genes in maize

JIA Liqiang¹(), ZHAO Qiufang², CHEN Shu², DING Bo¹^,^*()

1. School of Biological Sciences, Guizhou Education University, Guiyang 550018, China
2. South Subtropical Crops Research Institute, Chinese Academy of Tropical Agricultural Sciences, Zhanjiang 524091, Guangdong, China

Received:2021-05-19 Online:2022-02-25 Published:2022-03-02
Contact: DING Bo

Abstract

Abstract:

To explore the effect of various abiotic stresses on expression pattern of ZmbZIP, maize inbreed line Zheng58 was adopted as test materials, and five abiotic stresses (200 mmol·L ^-1 NaCl, 20% PEG6000, 4 ℃ low temperature, nitrate or ammonium deficiency stresses) were set, and expression of G group genes of bZIP family were systematically detected. Phylogenetic tree analysis showed that G group genes were subdivided into 3 subgroups. qRT-PCR analysis showed that 15 ZmbZIP genes had different expression pattern in various maize tissues, indicating their important roles during growth and development process in maize. Under different stresses of simulated salt, drought, low temperature and nitrogen deficiency, 20 ZmbZIP genes were widely regulated, indicating that most of those genes were involved in response to stress signaling pathway.

Key words: bZIP transcription factor, abiotic stresses, gene expression, maize

CLC Number:

S513

JIA Liqiang, ZHAO Qiufang, CHEN Shu, DING Bo. Expression analysis of bZIP G subfamily genes in maize[J]. Acta Agriculturae Zhejiangensis, 2022, 34(2): 221-231.

Figures/Tables 8

References 30

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基因名称 Gene name	基因号 Gene locus	上游引物 Forward primers (5'→3')	下游引物 Reverse primers (5'→3')
ZmbZIP2	Zm00001d028372	CATCCATCTGTAGCTCCAAGCCATC	CTGTCCCGTTTGGTAACTGCATAGG
ZmbZIP20	Zm00001d034571	GGCACGCTCAGATCAGAACTCGAC	ATGCTCAGAGTCGTCACAACGCTAG
ZmbZIP26	Zm00001d004897	CAGCAACGGGAAAGGAAGCCAC	GGCTGTGAAAACGCACTGGTTG
ZmbZIP39	Zm00001d005244	GGGAATGCTACCATTTTGCCACAAC	TTTCGCGCTTCAATTCCCTCTCATC
ZmbZIP40	Zm00001d040500	GCCAACACAGCAAGCTCTGCTC	CACGTTCATCCTGTATCCATTGCTC
ZmbZIP50	Zm00001d041142	CCCAGTGCTACAAATGTCGCGAAC	ACGAGCAGGCAGGAACAGCAAC
ZmbZIP51	Zm00001d043908	CCCTGGAATAGCGCCTGGTCATG	CTCCGCACTCAATGGGTTTACCAC
ZmbZIP67	Zm00001d043992	TCATCACGACCAGAGCCAAATCCA	ACCGCAATTGTTCCCGTGACGTTG
ZmbZIP73	Zm00001d052562	CCTATGCCAACAAATGGACATGCTG	CACTCTCACTCTCACTTCCACTCTC
ZmbZIP96	Zm00001d053967	CAATGAGCGTGGAGCCAGCCAA	TACATCGCTTGAGCCTTCTGCAC
ZmbZIP97	Zm00001d015118	CGTCTGCTCCATCCGCCAAC	AGAATCGCCATCTTCCTTCCCATC
ZmbZIP100	Zm00001d039065	GCATCGAGCCGCCGAAGTTG	TGCCTCCAGCACAGTTCCCATTG
ZmbZIP123	Zm00001d039206	CTCGTCCTGTCCGAGCACCTC	GTGAGTCCAACTACTGGCGGCTTAC
ZmbZIP133	Zm00001d019166	GGAAAGGAAGAAGGCGTACCTGAG	GCCTCTTCTGTTGTTGACAGTGGTG
ZmbZIP125	Zm00001d045288	GAACGGCCACACTTACCAGG	GATACGATCACGACGGCTCTAAACC
ZmbZIP136	Zm00001d047967	ATGGCGACGTTGGTGTGCCAC	GAGCGAGCCGTGATGGTGGTG
ZmbZIP138	Zm00001d046664	CAGCGTGGACCAGTTCTTCGAC	CATCGCCATCCACCTCCACCG
ZmbZIP140	Zm00001d024041	AGAACAACAACCGCAGACTCTCAAC	CCGCCTCCCACCTTCAACGAC
ZmbZIP142	Zm00001d024285	GCAACGCCTATACCGTTCTACCAG	CGGTGGATAATGGCAGGTCTGAG

基因名称 Gene name	基因号 Gene locus	上游引物 Forward primers (5'→3')	下游引物 Reverse primers (5'→3')
ZmbZIP2	Zm00001d028372	CATCCATCTGTAGCTCCAAGCCATC	CTGTCCCGTTTGGTAACTGCATAGG
ZmbZIP20	Zm00001d034571	GGCACGCTCAGATCAGAACTCGAC	ATGCTCAGAGTCGTCACAACGCTAG
ZmbZIP26	Zm00001d004897	CAGCAACGGGAAAGGAAGCCAC	GGCTGTGAAAACGCACTGGTTG
ZmbZIP39	Zm00001d005244	GGGAATGCTACCATTTTGCCACAAC	TTTCGCGCTTCAATTCCCTCTCATC
ZmbZIP40	Zm00001d040500	GCCAACACAGCAAGCTCTGCTC	CACGTTCATCCTGTATCCATTGCTC
ZmbZIP50	Zm00001d041142	CCCAGTGCTACAAATGTCGCGAAC	ACGAGCAGGCAGGAACAGCAAC
ZmbZIP51	Zm00001d043908	CCCTGGAATAGCGCCTGGTCATG	CTCCGCACTCAATGGGTTTACCAC
ZmbZIP67	Zm00001d043992	TCATCACGACCAGAGCCAAATCCA	ACCGCAATTGTTCCCGTGACGTTG
ZmbZIP73	Zm00001d052562	CCTATGCCAACAAATGGACATGCTG	CACTCTCACTCTCACTTCCACTCTC
ZmbZIP96	Zm00001d053967	CAATGAGCGTGGAGCCAGCCAA	TACATCGCTTGAGCCTTCTGCAC
ZmbZIP97	Zm00001d015118	CGTCTGCTCCATCCGCCAAC	AGAATCGCCATCTTCCTTCCCATC
ZmbZIP100	Zm00001d039065	GCATCGAGCCGCCGAAGTTG	TGCCTCCAGCACAGTTCCCATTG
ZmbZIP123	Zm00001d039206	CTCGTCCTGTCCGAGCACCTC	GTGAGTCCAACTACTGGCGGCTTAC
ZmbZIP133	Zm00001d019166	GGAAAGGAAGAAGGCGTACCTGAG	GCCTCTTCTGTTGTTGACAGTGGTG
ZmbZIP125	Zm00001d045288	GAACGGCCACACTTACCAGG	GATACGATCACGACGGCTCTAAACC
ZmbZIP136	Zm00001d047967	ATGGCGACGTTGGTGTGCCAC	GAGCGAGCCGTGATGGTGGTG
ZmbZIP138	Zm00001d046664	CAGCGTGGACCAGTTCTTCGAC	CATCGCCATCCACCTCCACCG
ZmbZIP140	Zm00001d024041	AGAACAACAACCGCAGACTCTCAAC	CCGCCTCCCACCTTCAACGAC
ZmbZIP142	Zm00001d024285	GCAACGCCTATACCGTTCTACCAG	CGGTGGATAATGGCAGGTCTGAG

基因ID Gene ID	基因名称 Gene name	长度 Length/bp	分子量 Molecular mass/ku	等电点 Isoelectric point	染色体 Chromosome	可变剪切 Splice variants
Zm00001d028372	ZmbZIP2	583	62.46	9.55	Chr1:32433751-32442505	17
Zm00001d034571	ZmbZIP20	356	37.47	7.59	Chr1:296596877-296600683	3
Zm00001d004897	ZmbZIP24	347	36.48	8.35	Chr2:147657998-147659916	1
Zm00001d005244	ZmbZIP26	371	38.75	7.16	Chr2:165521128-165527158	9
Zm00001d040500	ZmbZIP39	194	21.09	10.13	Chr3:47004098-47005326	1
Zm00001d041142	ZmbZIP40	382	40.63	8.41	Chr3:100232730-100240533	24
Zm00001d043908	ZmbZIP50	191	20.72	9.34	Chr3:213533138-213534279	1
Zm00001d043992	ZmbZIP51	340	37.03	8.8	Chr3:215850507-215866477	3
Zm00001d052562	ZmbZIP60	417	43.86	7.31	Chr4:193207888-193210831	1
Zm00001d053967	ZmbZIP67	314	33.62	6.17	Chr4:244414417-244418397	1
Zm00001d015118	ZmbZIP73	290	31.01	5.22	Chr5:76331375-76334353	1
Zm00001d039065	ZmbZIP96	377	40.12	7.51	Chr6:169491185-169496107	10
Zm00001d039206	ZmbZIP97	208	22.36	6.84	Chr6:172509105-172510659	1
Zm00001d019166	ZmbZIP100	379	39.68	6.88	Chr7:19967615-19972466	8
Zm00001d045288	ZmbZIP123	547	60.82	7.95	Chr9:18011858-18027409	1
Zm00001d047967	ZmbZIP133	423	45.11	9.94	Chr9:146611340-146618721	4
Zm00001d046664	ZmbZIP136	187	20.02	8.56	Chr10:5649486-5650525	1
Zm00001d024041	ZmbZIP138	282	29.93	7.12	Chr10:39073197-39078744	11
Zm00001d024285	ZmbZIP140	213	23.6	6.03	Chr10:61712124-61716615	1
Zm00001d025638	ZmbZIP142	273	30.15	8.45	Chr10:124742514-124747717	1

基因ID Gene ID	基因名称 Gene name	长度 Length/bp	分子量 Molecular mass/ku	等电点 Isoelectric point	染色体 Chromosome	可变剪切 Splice variants
Zm00001d028372	ZmbZIP2	583	62.46	9.55	Chr1:32433751-32442505	17
Zm00001d034571	ZmbZIP20	356	37.47	7.59	Chr1:296596877-296600683	3
Zm00001d004897	ZmbZIP24	347	36.48	8.35	Chr2:147657998-147659916	1
Zm00001d005244	ZmbZIP26	371	38.75	7.16	Chr2:165521128-165527158	9
Zm00001d040500	ZmbZIP39	194	21.09	10.13	Chr3:47004098-47005326	1
Zm00001d041142	ZmbZIP40	382	40.63	8.41	Chr3:100232730-100240533	24
Zm00001d043908	ZmbZIP50	191	20.72	9.34	Chr3:213533138-213534279	1
Zm00001d043992	ZmbZIP51	340	37.03	8.8	Chr3:215850507-215866477	3
Zm00001d052562	ZmbZIP60	417	43.86	7.31	Chr4:193207888-193210831	1
Zm00001d053967	ZmbZIP67	314	33.62	6.17	Chr4:244414417-244418397	1
Zm00001d015118	ZmbZIP73	290	31.01	5.22	Chr5:76331375-76334353	1
Zm00001d039065	ZmbZIP96	377	40.12	7.51	Chr6:169491185-169496107	10
Zm00001d039206	ZmbZIP97	208	22.36	6.84	Chr6:172509105-172510659	1
Zm00001d019166	ZmbZIP100	379	39.68	6.88	Chr7:19967615-19972466	8
Zm00001d045288	ZmbZIP123	547	60.82	7.95	Chr9:18011858-18027409	1
Zm00001d047967	ZmbZIP133	423	45.11	9.94	Chr9:146611340-146618721	4
Zm00001d046664	ZmbZIP136	187	20.02	8.56	Chr10:5649486-5650525	1
Zm00001d024041	ZmbZIP138	282	29.93	7.12	Chr10:39073197-39078744	11
Zm00001d024285	ZmbZIP140	213	23.6	6.03	Chr10:61712124-61716615	1
Zm00001d025638	ZmbZIP142	273	30.15	8.45	Chr10:124742514-124747717	1

Expression analysis of bZIP G subfamily genes in maize

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