Variation characteristics of soil microbial diversity in chrysanthemum rhizosphere soil with different continuous cropping years

doi:10.3969/j.issn.1004-1524.2022.12.14

Acta Agriculturae Zhejiangensis ›› 2022, Vol. 34 ›› Issue (12): 2710-2719.DOI: 10.3969/j.issn.1004-1524.2022.12.14

• Environmental Science • Previous Articles Next Articles

Variation characteristics of soil microbial diversity in chrysanthemum rhizosphere soil with different continuous cropping years

ZHANG Xiaobo(), YU Chunlei, ZHANG Wenyang, YAN Ye, RUAN Fang()

Liaoning Research Institute of Cash Crops, Liaoyang 111000, Liaoning, China

Received:2022-03-16 Online:2022-12-25 Published:2022-12-26
Contact: RUAN Fang

Abstract

Abstract:

In order to reveal the community structure and diversity characteristics of microbe in chrysanthemum rhizosphere soil under continuous cropping, taking the rhizosphere soil of chrysanthemum with different continuous cropping years as the research object, high-throughput sequencing technology (Illumina-MiSeq) and bioinformatics were used to analyze the bacterial and fungal community composition and diversity in chrysanthemum rhizosphere soil of no continuous cropping (CK), continuous cropping for 2-years (2a) and continuous cropping for 9 years (9a). The results showed that the number and diversity of bacteria in rhizosphere soil of chrysanthemum in 2a and 9a treatments were significantly (P<0.05) higher than those of CK. Continuous cropping had little influence on the fungal community diversity and abundance, but had significant influence on the fungal community composition. Zygomycota, Basidiomycota, Mortierella and other microbial communities were decreased, and the abundance of microbial communities such as Monographella, Fusarium, Ilyonectria and other microbial community were increased in 2a and 9a treatment, compared with CK. Continuous cropping of chrysanthemum significantly increased the number and diversity of bacteria, and significantly affected the composition of fungal community in the rhizosphere soil.

Key words: chrysanthemum, continuous cropping, rhizosphere soil, bacteria, fungal

CLC Number:

S154.3

ZHANG Xiaobo, YU Chunlei, ZHANG Wenyang, YAN Ye, RUAN Fang. Variation characteristics of soil microbial diversity in chrysanthemum rhizosphere soil with different continuous cropping years[J]. Acta Agriculturae Zhejiangensis, 2022, 34(12): 2710-2719.

Figures/Tables 6

Fig.1 Venn diagram of bacteria (A) and fungal (B) communities in chrysanthemum rhizosphere soil under different continuous cropping years

Table 1 Richness and diversity indices of bacteria and fungal communities in chrysanthemum rhizosphere soil under different continuous cropping years

微生物类群 Microbial groups	处理 Treatment	覆盖度 Coverage	Ace指数 Ace index	Chao指数 Chao index	Shannon指数 Shannon index	Simpson指数 Simpson index
细菌Bacteria	CK	0.992 0	4 691.305 2 b	4 645.523 2 b	6.640 4 b	0.005 2 a
	2a	0.993 4	5 553.566 7 a	5 465.612 0 a	6.953 1 a	0.004 2 a
	9a	0.991 8	5 270.647 6 a	5 177.709 1 a	6.742 5 b	0.004 7 a
真菌Fungi	CK	0.999 6	729.559 3 a	728.330 5 a	3.125 7 a	0.118 7 a
	2a	0.999 8	848.267 0 a	846.308 7 a	3.746 6 a	0.064 0 a
	9a	0.999 7	728.287 6 a	729.722 2 a	3.193 0 a	0.145 0 a

Fig.2 Heatmap diagram of bacteria (A) and fungal (B) communitiesin chrysanthemum rhizosphere soil under different continuous cropping years at genus level

Fig.3 Principal coordinate analysis of bacteria (A) and fungal (B) in chrysanthemum rhizosphere soil under different continuous cropping years

Fig.4 Composition and relative abundance of bacteria (A) and fungal (B) in chrysanthemum rhizosphere soil under different continuous cropping years at phylum level

Fig.5 Composition and relative abundance of bacteria(A) and fungal(B) in chrysanthemum rhizosphere soil under different continuous cropping years at genus level

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Variation characteristics of soil microbial diversity in chrysanthemum rhizosphere soil with different continuous cropping years

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