Isolation and identification of main postharvest pathogens of four edible fungi

doi:10.3969/j.issn.1004-1524.20240313

Acta Agriculturae Zhejiangensis ›› 2025, Vol. 37 ›› Issue (2): 456-465.DOI: 10.3969/j.issn.1004-1524.20240313

• Food Science • Previous Articles Next Articles

Isolation and identification of main postharvest pathogens of four edible fungi

GONG Xinxin¹^,²(), LIU Ruiling², HAN Yanchao², MENG Xianghong¹, GAO Haiyan²^,^*(), CHEN Hangjun²^,^*()

1. College of Food Science and Engineering, Ocean University of China, Qingdao 266100, Shandong, China
2. Key Laboratory of Postharvest Vegetable Preservation and Processing of the Ministry of Agriculture and Rural Affairs (Co-Constructed by the Ministry and Province), Key Laboratory of Post-Harvest Handling of Fruits, Ministry of Agriculture and Rural Affairs, Zhejiang Key Laboratory of Intelligent Food Logistic and Processing, Key Laboratory of Postharvest Preservation and Processing of Fruits and Vegetables, China National Light Industry, Institute of Food Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Received:2024-04-03 Online:2025-02-25 Published:2025-03-20
Contact: GAO Haiyan,CHEN Hangjun

Abstract

Abstract:

In this paper, Lentinus edodes, Agaricus bisporus, Auricularia auricula, and Pleurotus pulmonarius were chosen as test materials to explore the main postharvest pathogens of edible fungi in Zhejiang Province of China. The pathogens were isolated and purified using tissue isolation and plate delineation methods, and they were identified morphologically and molecularly. Trichoderma harzianum, Trichoderma virens, Aspergillus tubingensis, and Pseudomonas putida were the main pathogens of the test fungi, according to sequence comparison and phylogenetic tree analysis. The identification of the main postharvest pathogens affecting edible fungi could serve as the foundation for their preservation and control strategies, to ensure the timely safety of edible fungi products.

Key words: edible fungi, postharvest disease, pathogenic fungi, pathogenic bacteria, isolation and identification

CLC Number:

S436.46

GONG Xinxin, LIU Ruiling, HAN Yanchao, MENG Xianghong, GAO Haiyan, CHEN Hangjun. Isolation and identification of main postharvest pathogens of four edible fungi[J]. Acta Agriculturae Zhejiangensis, 2025, 37(2): 456-465.

Figures/Tables 6

References 25

[1]	鲍大鹏, 邹根, 裴晓东, 等. 中国食用菌产业实现高质量现代化发展的路径探讨[J]. 食用菌学报, 2022, 29(6): 103-110.
	BAO D P, ZOU G, PEI X D, et al. Discussion on the path toward high quality modernization of edible fungi industry in China[J]. Acta Edulis Fungi, 2022, 29(6): 103-110. (in Chinese)
[2]	XUE Z H, HAO J F, YU W C, et al. Effects of processing and storage preservation technologies on nutritional quality and biological activities of edible fungi: a review[J]. Journal of Food Process Engineering, 2017, 40(3): e12437.
[3]	KIM S W, KIM S, LEE H J, et al. Isolation of fungal pathogens to an edible mushroom, Pleurotus eryngii, and development of specific ITS primers[J]. Mycobiology, 2013, 41(4): 252-255.
[4]	冯智红, 严培兰, 王建华, 等. 香菇中污染真菌的分离与鉴定[J]. 上海农业学报, 2015, 31(4): 50-53.
	FENG Z H, YAN P L, WANG J H, et al. Isolation and identification of fungus contaminated in Lentinula edodes[J]. Acta Agriculturae Shanghai, 2015, 31(4): 50-53. (in Chinese with English abstract)
[5]	李昊珍, 刘瑞玲, 牛犇, 等. 双孢蘑菇采后主要病原菌分离鉴定及生长特性研究[J]. 浙江农业科学, 2023, 64(6): 1534-1538.
	LI H Z, LIU R L, NIU B, et al. Identification and growth characteristics of dominant pathogens of postharvest Agaricus bisporus[J]. Journal of Zhejiang Agricultural Sciences, 2023, 64(6): 1534-1538. (in Chinese with English abstract)
[6]	刘绍雄, 刘春丽, 尚陆娥, 等. 云南黑木耳常见病害调查及病原菌分离与鉴定[J]. 中国食用菌, 2019, 38(11): 89-94.
	LIU S X, LIU C L, SHANG L E, et al. Investigation on several common diseases of Auricularia heimuer in Yunnan and isolation and identification of pathogens[J]. Edible Fungi of China, 2019, 38(11): 89-94. (in Chinese with English abstract)
[7]	郭倩, 周昌艳, 谭琦, 等. 华东地区秀珍菇黄菇病的发生及防治[J]. 中国食用菌, 2004, 23(1): 30-31.
	GUO Q, ZHOU C Y, TAN Q, et al. Occurrence and control of Pleurotus eryngii yellow mushroom disease in East China[J]. Edible Fungi of China, 2004, 23(1): 30-31. (in Chinese)
[8]	袁卫东, 陆娜, 宋吉玲, 等. 浙江秀珍菇黄菇病病原菌的分离与鉴定[J]. 浙江农业学报, 2018, 30(11): 1893-1898.
	YUAN W D, LU N, SONG J L, et al. Pathogen isolation and identification of yellow rot disease in Pleurotus geesteranus in Zhejiang Province[J]. Acta Agriculturae Zhejiangensis, 2018, 30(11): 1893-1898. (in Chinese with English abstract)
[9]	LU N, YUAN W D, WU Y L, et al. Identification of contaminants as members of the Bacillus pumilus group and analysis of contamination sources in the cultivation of Pleurotus geesteranus mushrooms[J]. Journal of Plant Pathology, 2021, 103(1): 105-115.
[10]	卢政辉. 秀珍菇哈茨木霉菌高效低毒防治药剂的筛选[J]. 食药用菌, 2018, 26(3): 178-180, 183.
	LU Z H. Screening of efficient and low toxic agent to control Trichoderma harzianum of Pleurotus pulmonariusa[J]. Edible and Medicinal Mushrooms, 2018, 26(3): 178-180, 183. (in Chinese with English abstract)
[11]	王晶. 食用菌病害的调查与新病害病原鉴定研究[D]. 沈阳: 沈阳农业大学, 2010.
	WANG J. Investigation of edible fungi diseases and identification of new pathogens[D]. Shenyang: Shenyang Agricultural University, 2010. (in Chinese with English abstract)
[12]	杜小琴, 李杰, 秦文, 等. 甜樱桃果实采后病原菌的分离鉴定及其生物学特性研究[J]. 食品工业科技, 2015, 36(18): 197-202.
	DU X Q, LI J, QIN W, et al. Isolation, identification and biological characteristics of pathogenic bacteria for sweet cherry[J]. Science and Technology of Food Industry, 2015, 36(18): 197-202. (in Chinese with English abstract)
[13]	方中达. 植病研究方法[M]. 3版. 北京: 中国农业出版社, 1998.
[14]	UZAIR B, MENAA F, KHAN B A, et al. Isolation, purification, structural elucidation and antimicrobial activities of kocumarin, a novel antibiotic isolated from actinobacterium Kocuria marina CMG S2 associated with the brown seaweed Pelvetia canaliculata[J]. Microbiological Research, 2018, 206: 186-197.
[15]	牛天贵. 食品微生物学实验技术[M]. 2版. 北京: 中国农业大学出版社, 2011.
[16]	魏景超. 真菌鉴定手册[M]. 上海: 上海科学技术出版社,1979.
[17]	罗秀俊, 郭建峰, 韩波波, 等. 一株香菇病原真菌的分离、鉴定与室内防治药剂筛选[J]. 云南农业大学学报(自然科学), 2017, 32(5): 793-804.
	LUO X J, GUO J F, HAN B B, et al. Isolation, identification of a Lentinula edodes pathogen and vitro screening of control medicament[J]. Journal of Yunnan Agricultural University(Natural Science), 2017, 32(5): 793-804. (in Chinese with English abstract)
[18]	王莹, 刘作易, 冯瑶, 等. 香菇菌种生产中木霉侵染过程研究[J]. 种子, 2021, 40(6): 131-141.
	WANG Y, LIU Z Y, FENG Y, et al. Study on Trichoderma infection process in Lentinus edodes strain production[J]. Seed, 2021, 40(6): 131-141. (in Chinese with English abstract)
[19]	宋晓霞, 王倩, 隽加香, 等. 双孢蘑菇工厂化栽培中四种木霉病害的精准鉴定[J]. 食用菌, 2019, 41(3): 67-72.
	SONG X X, WANG Q, JUN J X, et al. Study on accurate identification of four Trichoderma diseases in Agaricus bisporus in factory cultivation[J]. Edible Fungi, 2019, 41(3): 67-72. (in Chinese with English abstract)
[20]	刘正慧, 李丹, SOSSAH Frederick Leo, 等. 食用菌主要病原真菌和细菌[J]. 菌物研究, 2018, 16(3): 158-163.
	LIU Z H, LI D, LEO S, et al. Major pathogenic fungi and bacteria in edible fungi[J]. Journal of Fungal Research, 2018, 16(3): 158-163. (in Chinese with English abstract)
[21]	李婕, 黄琳翔, 詹艺舒, 等. 双孢蘑菇贮藏保鲜过程中菇体表面的菌相变化[J]. 食品工业科技, 2019, 40(9): 109-114.
	LI J, HUANG L X, ZHAN Y S, et al. Changes of microflora on the surface of Agaricus bisporus fruit body during chilled storage[J]. Science and Technology of Food Industry, 2019, 40(9): 109-114. (in Chinese with English abstract)
[22]	高源, 周春香, 杨丽芬, 等. 小桐子叶斑病菌塔宾曲霉的生物学特性研究[J]. 云南农业大学学报(自然科学), 2017, 32(4): 600-605.
	GAO Y, ZHOU C X, YANG L F, et al. Study on biological characteristics of Aspergillus tubingensis causing leaf spot disease of Jatropha curcas[J]. Journal of Yunnan Agricultural University(Natural Science), 2017, 32(4): 600-605. (in Chinese with English abstract)
[23]	GUO J W, GAO Y, LI C Y, et al. First report of leaf spot disease caused by Aspergillus tubingensis on Jatropha curcas in Yunnan, China[J]. Plant Disease, 2017, 101(3): 505.
[24]	DIGUTA C F, VINCENT B, GUILLOUX-BENATIER M, et al. PCR ITS-RFLP: a useful method for identifying filamentous fungi isolates on grapes[J]. Food Microbiology, 2011, 28(6): 1145-1154.
[25]	衣杰. 几种常见食用菌细菌性病害的诊断及防治(二): 蘑菇、平菇、香菇[J]. 中国食用菌, 2005, 24(3): 41.
	YI J. Diagnosis and control of bacterial diseases of several common edible fungi (Ⅱ): mushrooms, oyster mushroon and Lentinula edodes[J]. Edible Fungi of China, 2005, 24(3): 41. (in Chinese)

名称 Name	序列 Sequence
XG-1	TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGTTTACAACTCCCAAACCCAATGTGAACGTTACCAAACTGTTGCCTCGGCGGG- ATCTCTGCCCCGGGTGCGTCGCAGCCCCGGACCAAGGCGCCCGCCGGAGGACCAACCAAAACTCTTTTTGTATACCCCCTCGCGGGT- TTTTTATAATCTGAGCCTTCTCGGCGCCTCTCGTAGGCGTTTCGAAAATGAATCAAAACTTTCAACAACGGATCTCTTGGTTCTGGCAT- CGATGAAGAACGCAGCGAAATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCGC- CAGTATTCTGGCGGGCATGCCTGTCCGAGCGTCATTTCAACCCTCGAACCCCTCCGGGGGGTCGGCGTTGGGGATCGGCCCTGCCTTT- GGCGGTGGCCGTCTCCGAAATACAGTGGCGGTCTCGCCGCAGCCTCTCCTGCGCAGTAGTTTGCACACTCGCATCGGGAGCGCGGCG- CGTCCACAGCCGTTAAACACCCAACTTCTGAAATGTTGACCTCGGATCAGGTAGGAATACCCGCTGAACTTAAGCATATCAATAAGC- GGAGGAA
SB-1	TGGAAGTTAAAAAAACGAAACAAGGTCTCCGTTGGTGAACCAGCGGAGGGATCATTACCGAGTTTACAACTCCCAAACCCAATGTG- AACGTTACCAAACTGTTGCCTCGGCGGGATCTCTGCCCCGGGTGCGTCGCAGCCCCGGACCAAGGCGCCCGCCGGAGGACCAACCA- AAACTCTTATTGTATACCCCCTCGCGGGTTTTTTACTATCTGAGCCATCTCGGCGCCCCTCGTGGGCGTTTCGAAAATGAATCAAAAC- TTTCAACAACGGATCTCTTGGTTCTGGCATCGATGAAGAACGCAGCGAAATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATC- ATCGAATCTTTGAACGCACATTGCGCCCGCCAGTATTCTGGCGGGCATGCCTGTCCGAGCGTCATTTCAACCCTCGAACCCCTCCGG- GGGGTCGGCGTTGGGGATCGGCCCTTTACGGGGCCGGCCCCGAAATACAGTGGCGGTCTCGCCGCAGCCTCTCCTGCGCAGTAGTTT- GCACACTCGCATCGGGAGCGCGGCGCGTCCACAGCCGTTAAACACCCCAAACTTCTGAAATGTGACCTCGGATCAGGTAGGAATTC- CCAG
ME-1	CTTCCGTAGGTGAACCTGCGGAAGGATCATTACCGAGTGCGGGTCCTTTGGGCCCAACCTCCCATCCGTGTCTATTATACCCTGTTGC- TTCGGCGGGCCCGCCGCTTGTCGGCCGCCGGGGGGGCGCCTTTGCCCCCCGGGCCCGTGCCCGCCGGAGACCCCAACACGAACACT- GTCTGAAAGCGTGCAGTCTGAGTTGATTGAATGCAATCAGTTAAAACTTTCAACAATGGATCTCTTGGTTCCGGCATCGATGAAGAA- CGCAGCGAAATGCGATAACTAATGTGAATTGCAGAATTCAGTGAATCATCGAGTCTTTGAACGCACATTGCGCCCCCTGGTATTCCG- GGGGGCATGCCTGTCCGAGCGTCATTGCTGCCCTCAAGCCCGGCTTGTGTGTTGGGTCGCCGTCCCCCTCTCCGGGGGGACGGGCCC- GAAAGGCAGCGGCGGCACCGCGTCCGATCCTCGAGCGTATGGGGCTTTGTCACATGCTCTGTAGGATTGGCCGGCGCCTGCCGACGT- TTTCCAACCATTTTTTCCAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAA
XZ-1	GAGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAGCGGATGACGGGAGCTTGCTCCTTGATT- CAGCGGCGGACGGGTGAGTAATGCCTAGGAATCTGCCTGGTAGTGGGGGACAACGTTTCGAAAGGAACGCTAATACCGCATACGTC- CTACGGGAGAAAGCAGGGGACCTTCGGGCCTTGCGCTATCAGATGAGCCTAGGTCGGATTAGCTAGTTGGTGGGGTAATGGCTCAC- CAAGGCGACGATCCGTAACTGGTCTGAGAGGATGATCAGTCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAG- TGGGGAATATTGGACAATGGGCGAAAGCCTGATCCAGCCATGCCGCGTGTGTGAAGAAGGTCTTCGGATTGTAAAGCACTTTAAGTT- GGGAGGAAGGGCAGTAAGCTAATACCTTGCTGTTTTGACGTTACCGACAGAATAAGCACCGGCTAACTCTGTGCCAGCAGCCGCGGT- AATACAGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTAGGTGGTTCGTTAAGTTGGATGTGAAAGCCCCGGG- CTCAACCTGGGAACTGCATCCAAAACTGGCGAGCTAGAGTACGGTAGAGGGTGGTGGAATTTCCTGTGTAGCGGTGAAATGCGTAG- ATATAGGAAGGAACACCAGTGGCGAAGGCGACCACCTGGACTGATACTGACACTGAGGTGCGAAAGCGTGGGGAGCAAACAGGAT- TAGATACCCTGGTAGTCCACGCCGTAAACGATGTCAACTAGCCGTTGGAATCCTTGAGATTTTAGTGGCGCAGCTAACGCATTAAGT- TGACCGCCTGGGGAGTACGGCCGCAAGGTTAAAACTCAAATGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATT- CGAAGCAACGCGAAGAACCTTACCAGGCCTTGACATGCAGAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTCTGACACAG- GTGCTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGTAACGAGCGCAACCCTTGTCCTTAGTTACCAGCACG- TTATGGTGGGCACTCTAAGGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAGTCATCATGGCCCTTACGGCCTG- GGCTACACACGTGCTACAATGGTCGGTACAGAGGGTTGCCAAGCCGCGAGGTGGAGCTAATCTCACAAAACCGATCGTAGTCCGGA- TCGCAGTCTGCAACTCGACTGCGTGAAGTCGGAATCGCTAGTAATCGCGAATCAGAATGTCGCGGTGAATACGTTCCCGGGCCTTGT- ACACACCGCCCGTCACACCATGGGAGTGGGTTGCACCAGAAGTAGCTAGTCTAACCTTCGGGAGGACGGTTACCACGGTGTGATTC- ATGACTGGGGTGAAG

名称 Name	序列 Sequence
XG-1	TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGTTTACAACTCCCAAACCCAATGTGAACGTTACCAAACTGTTGCCTCGGCGGG- ATCTCTGCCCCGGGTGCGTCGCAGCCCCGGACCAAGGCGCCCGCCGGAGGACCAACCAAAACTCTTTTTGTATACCCCCTCGCGGGT- TTTTTATAATCTGAGCCTTCTCGGCGCCTCTCGTAGGCGTTTCGAAAATGAATCAAAACTTTCAACAACGGATCTCTTGGTTCTGGCAT- CGATGAAGAACGCAGCGAAATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCGC- CAGTATTCTGGCGGGCATGCCTGTCCGAGCGTCATTTCAACCCTCGAACCCCTCCGGGGGGTCGGCGTTGGGGATCGGCCCTGCCTTT- GGCGGTGGCCGTCTCCGAAATACAGTGGCGGTCTCGCCGCAGCCTCTCCTGCGCAGTAGTTTGCACACTCGCATCGGGAGCGCGGCG- CGTCCACAGCCGTTAAACACCCAACTTCTGAAATGTTGACCTCGGATCAGGTAGGAATACCCGCTGAACTTAAGCATATCAATAAGC- GGAGGAA
SB-1	TGGAAGTTAAAAAAACGAAACAAGGTCTCCGTTGGTGAACCAGCGGAGGGATCATTACCGAGTTTACAACTCCCAAACCCAATGTG- AACGTTACCAAACTGTTGCCTCGGCGGGATCTCTGCCCCGGGTGCGTCGCAGCCCCGGACCAAGGCGCCCGCCGGAGGACCAACCA- AAACTCTTATTGTATACCCCCTCGCGGGTTTTTTACTATCTGAGCCATCTCGGCGCCCCTCGTGGGCGTTTCGAAAATGAATCAAAAC- TTTCAACAACGGATCTCTTGGTTCTGGCATCGATGAAGAACGCAGCGAAATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATC- ATCGAATCTTTGAACGCACATTGCGCCCGCCAGTATTCTGGCGGGCATGCCTGTCCGAGCGTCATTTCAACCCTCGAACCCCTCCGG- GGGGTCGGCGTTGGGGATCGGCCCTTTACGGGGCCGGCCCCGAAATACAGTGGCGGTCTCGCCGCAGCCTCTCCTGCGCAGTAGTTT- GCACACTCGCATCGGGAGCGCGGCGCGTCCACAGCCGTTAAACACCCCAAACTTCTGAAATGTGACCTCGGATCAGGTAGGAATTC- CCAG
ME-1	CTTCCGTAGGTGAACCTGCGGAAGGATCATTACCGAGTGCGGGTCCTTTGGGCCCAACCTCCCATCCGTGTCTATTATACCCTGTTGC- TTCGGCGGGCCCGCCGCTTGTCGGCCGCCGGGGGGGCGCCTTTGCCCCCCGGGCCCGTGCCCGCCGGAGACCCCAACACGAACACT- GTCTGAAAGCGTGCAGTCTGAGTTGATTGAATGCAATCAGTTAAAACTTTCAACAATGGATCTCTTGGTTCCGGCATCGATGAAGAA- CGCAGCGAAATGCGATAACTAATGTGAATTGCAGAATTCAGTGAATCATCGAGTCTTTGAACGCACATTGCGCCCCCTGGTATTCCG- GGGGGCATGCCTGTCCGAGCGTCATTGCTGCCCTCAAGCCCGGCTTGTGTGTTGGGTCGCCGTCCCCCTCTCCGGGGGGACGGGCCC- GAAAGGCAGCGGCGGCACCGCGTCCGATCCTCGAGCGTATGGGGCTTTGTCACATGCTCTGTAGGATTGGCCGGCGCCTGCCGACGT- TTTCCAACCATTTTTTCCAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAA
XZ-1	GAGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAGCGGATGACGGGAGCTTGCTCCTTGATT- CAGCGGCGGACGGGTGAGTAATGCCTAGGAATCTGCCTGGTAGTGGGGGACAACGTTTCGAAAGGAACGCTAATACCGCATACGTC- CTACGGGAGAAAGCAGGGGACCTTCGGGCCTTGCGCTATCAGATGAGCCTAGGTCGGATTAGCTAGTTGGTGGGGTAATGGCTCAC- CAAGGCGACGATCCGTAACTGGTCTGAGAGGATGATCAGTCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAG- TGGGGAATATTGGACAATGGGCGAAAGCCTGATCCAGCCATGCCGCGTGTGTGAAGAAGGTCTTCGGATTGTAAAGCACTTTAAGTT- GGGAGGAAGGGCAGTAAGCTAATACCTTGCTGTTTTGACGTTACCGACAGAATAAGCACCGGCTAACTCTGTGCCAGCAGCCGCGGT- AATACAGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTAGGTGGTTCGTTAAGTTGGATGTGAAAGCCCCGGG- CTCAACCTGGGAACTGCATCCAAAACTGGCGAGCTAGAGTACGGTAGAGGGTGGTGGAATTTCCTGTGTAGCGGTGAAATGCGTAG- ATATAGGAAGGAACACCAGTGGCGAAGGCGACCACCTGGACTGATACTGACACTGAGGTGCGAAAGCGTGGGGAGCAAACAGGAT- TAGATACCCTGGTAGTCCACGCCGTAAACGATGTCAACTAGCCGTTGGAATCCTTGAGATTTTAGTGGCGCAGCTAACGCATTAAGT- TGACCGCCTGGGGAGTACGGCCGCAAGGTTAAAACTCAAATGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATT- CGAAGCAACGCGAAGAACCTTACCAGGCCTTGACATGCAGAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTCTGACACAG- GTGCTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGTAACGAGCGCAACCCTTGTCCTTAGTTACCAGCACG- TTATGGTGGGCACTCTAAGGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAGTCATCATGGCCCTTACGGCCTG- GGCTACACACGTGCTACAATGGTCGGTACAGAGGGTTGCCAAGCCGCGAGGTGGAGCTAATCTCACAAAACCGATCGTAGTCCGGA- TCGCAGTCTGCAACTCGACTGCGTGAAGTCGGAATCGCTAGTAATCGCGAATCAGAATGTCGCGGTGAATACGTTCCCGGGCCTTGT- ACACACCGCCCGTCACACCATGGGAGTGGGTTGCACCAGAAGTAGCTAGTCTAACCTTCGGGAGGACGGTTACCACGGTGTGATTC- ATGACTGGGGTGAAG

Isolation and identification of main postharvest pathogens of four edible fungi

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 6

References 25

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	YANG Lei, WANG Xiaofu, WEI Wei, CHEN Xiaoyun, PENG Cheng, XU Xiaoli, XU Junfeng. The antifungal responses of insects against an entomopathogenic fungi, Beauveria bassiana and their application potential in pest control [J]. Acta Agriculturae Zhejiangensis, 2024, 36(4): 825-836.
[2]	YE Tao, SUN Qinyu, CHEN Weili, SHAN Wenshu, LIAN Wenxu, NIU Tingting, ZHANG Jiaxia. Research progress of bZIP gene family in plant-pathogenic fungi [J]. Acta Agriculturae Zhejiangensis, 2024, 36(12): 2885-2894.
[3]	JIA Beiping, LYU Xuan, YANG Qing, WANG Yinan, LI Wanxiao, XIE Xindi, ZHU Yingqi, WANG Bei, YIN Dongdong, ZHANG Yunkai, WANG Qing, WANG Guijun. Isolation, identification and genetic evolution analysis of novel goose astrovirus in Anhui Province, China [J]. Acta Agriculturae Zhejiangensis, 2023, 35(5): 1048-1057.
[4]	WANG Zhipeng, ZHAO Jian, HUANG Pan, CUI Xuemei, NAN Li, SONG Houhui, BAO Guolian, LIU Yan. Isolation, identification and biological characteristics of rabbit-derived Escherichia coli bacteriophage [J]. Acta Agriculturae Zhejiangensis, 2022, 34(8): 1599-1608.
[5]	LI Xudong, LIU Yongtao, YANG Xianle, YANG Yibin, AI Xiaohui. Analysis on pathogens of frogs with crooked head, broken head or white eye [J]. Acta Agriculturae Zhejiangensis, 2022, 34(8): 1617-1625.
[6]	WANG Xiaoli, ZHAO Yingwei, KONG Xiaona, CAO Zilin. Isolation and identification of mycorrhizal fungi in rhizosphere and their effect on growth and photosynthetic characteristics of Eucalyptus globulus seedlings [J]. Acta Agriculturae Zhejiangensis, 2022, 34(5): 1015-1023.
[7]	ZHU Yinchu, WANG Hongyu, YUN Tao, HUA Jionggang, YE Weicheng, NI Zheng, CHEN Liu, ZHANG Cun. Isolation and identification of goose astrovirus in Zhejiang Province, China, and preparation of polyclone antibodies against capside protein [J]. Acta Agriculturae Zhejiangensis, 2022, 34(10): 2149-2159.
[8]	CHEN Mengzhu, KANG Zhenya, GUO Xianghui, GENG Yi, BAI Minghuan, OUYANG Ping, CHEN Defang, HUANG Xiaoli, LAI Weimin. Isolation and biological characteristics of a pathogenic ST-251 Aeromonas hydrophila from Procypris Rabaudi (rock carp) [J]. Acta Agriculturae Zhejiangensis, 2021, 33(12): 2286-2294.
[9]	YUAN Xianyu, YANG Longbin, HE Zanzan, MAO Tianjiao, HE Changsheng, ZHAN Songhe, SUN Pei, WEI Jianzhong, LI Yu. Isolation and identification of pseudorabies virus and molecular characterization of its main virulence genes in Anhui [J]. , 2020, 32(1): 43-56.
[10]	YI Keke, YIN Wenqi, ZHOU Yuancheng, JIANG Jinzhen, ZHANG Baiyu, LI Zhongyin, YAN Qigui. Isolation and identification of 4 strains of porcine pseudorabies virus and analysis of main virulence genes [J]. , 2019, 31(9): 1429-1436.
[11]	YANG Yibin, AI Xiaohui, SONG Yi, DONG Jing, XU Ning, JIANG Lan. Preliminary study on hemolytic ascites disease of Pelteobagrus fulvidraco [J]. , 2019, 31(8): 1239-1248.
[12]	CUI Yilong, SHI Yun, YANG Dahan, YIN Youqin, XUE Jiangdong, HUO Xiaowei, MA Dehui. Isolation,identification of horse Bacillus cereus and its virulence genes detection [J]. , 2019, 31(2): 216-221.
[13]	BA Shaobo, SHI Lin, LI Qunjing, LIU Zhengkui, CHEN Lin, WANG Xiaodu, SONG Houhui. Isolation, identification and molecullar epidemiological analysis of 17-ZJ-HZ strain of porcine reproductive and respiratory syndrome virus [J]. , 2018, 30(8): 1303-1311.
[14]	YANG Yibin, SONG Yi, YANG Qiuhong, LIU Yongtao, YANG Xianle, AI Xiaohui. Isolation, identification and antibiotic sensitivity of Morganella morganii from Channa argus [J]. , 2018, 30(2): 194-202.
[15]	ZHENG Liping, GENG Yi, LEI Xueping, YU Zehui, HUANG Xiaoli, CHEN Defang, OUYANG Ping, CAO Shiqi, HAN Rui. Isolation and identification of Streptococcus iniae from sturgeon and its pathological lesions of infection [J]. , 2018, 30(2): 203-210.