Effects of interstock on leaf photosynthetic characteristics, physicochemical properties and fruit quality of three mandarin hybrids

doi:10.3969/j.issn.1004-1524.20241017

Acta Agriculturae Zhejiangensis ›› 2025, Vol. 37 ›› Issue (8): 1680-1693.DOI: 10.3969/j.issn.1004-1524.20241017

• Horticultural Science • Previous Articles Next Articles

Effects of interstock on leaf photosynthetic characteristics, physicochemical properties and fruit quality of three mandarin hybrids

HE Shixiong¹^,²(), YANG Lei¹, QI Anmin³, CHENG Ji⁴, WANG Min¹, LI Yingkui¹, HONG Lin¹^,^*()

1. Fruit Research Institute, Chongqing Academy of Agricultural Sciences, Chongqing 401329, China
2. Guang’an Academy of Agriculture and Forestry Sciences, Guang’an 638000, Sichuan, Sichuan, China
3. Yunyang County Development Center of Fruit Industry, Chongqing 404500, China
4. Kaizhou District Agricultural Development Service Center, Chongqing 405400, China

Received:2024-11-25 Online:2025-08-25 Published:2025-09-03
Contact: HONG Lin

Abstract

Abstract:

To evaluate the effects of interstock on the photosynthesis, physiological and biochemical properties, mineral elements, and fruit quality of three mandarin hybrids, using Licheng(single interstock) and Licheng/Ehime 28 (combined interstock) as interstocks for grafting the Tango, Harumi and Orah cultivars, we analyzed leaf photosynthetic pigments contents, soluble sugar content, starch content, diurnal photosynthetic variation, stress-related enzyme activity, mineral element content, as well as fruit quality indicators such as soluble solids, titratable acidity, and vitamin C content. Principal component analysis(PCA) was used to evaluate the overall performance of each scion-rootstock combination. The results showed that photosynthetic efficiency varied significantly among the different varieties, while the diurnal curves of net photosynthetic rate (P_n), stomatal conductance (G_s), transpiration rate (T_r) and intercellular CO₂ concentration (C_i) showed no significant differences among varieties with different interstocks, but the combined interstock significantly increased the P_n of Orah and Harumi in the morning, with peak values being 13.40% and 10.85% higher, respectively. The interstock type influenced the chlorophyll a, chlorophyll b, and total chlorophyll content in the leaves of the hybrid citrus varieties. The soluble sugar and starch contents in the leaves of all three varieties were higher on the combined interstock than those on the single interstock. The combined interstock increased the accumulation of total phenols and soluble proteins in the leaves while reducing phenylalanine ammonia lyase (PAL) activity. Compared with activities of superoxide dismutase (SOD), peroxidase (POD), and polyphenol oxidase (PPO), the activities of catalase (CAT), β-1,3-glucanase (β-1,3-GA), exochitinase and endochitinase were more significantly affected by the interstock treatment. Compared with the single interstock, the combined interstock increased the content of nitrogen (N), phosphorus (P), potassium (K), zinc (Zn), and boron (B) in the leaves of Orah and Harumi while decreased the content of calcium (Ca) and magnesium (Mg). The variation in the micronutrient iron(Fe) content was the most pronounced between the two interstocks, followed by Zn and B. Additionally, the soluble solids contents in the fruits of Tango and Harumi were higher on the single interstock, whereas the combined interstock resulted in lower titratable acidity and vitamin C content but a higher solid-acid ratio in all three varieties. The different interstocks had varying effects on the photosynthesis, physiological biochemistry, stress resistance-related enzyme activity, mineral element accumulation, and fruit quality of the grafted varieties. The combined interstock enhanced the accumulation of soluble sugars, starch, and total phenols while reduced PAL activity, which resulted in lower fruit acidity. Through comprehensive evaluation, it was found that Harumi and Orah grafted on the combined interstock performed better than those on the single interstock, whereas Tango showed the opposite trend.

Key words: interstock, mandarin hybrid, physicochemical property, photosynthetic characteristic, mineral element, fruit quality

CLC Number:

S666

HE Shixiong, YANG Lei, QI Anmin, CHENG Ji, WANG Min, LI Yingkui, HONG Lin. Effects of interstock on leaf photosynthetic characteristics, physicochemical properties and fruit quality of three mandarin hybrids[J]. Acta Agriculturae Zhejiangensis, 2025, 37(8): 1680-1693.

Figures/Tables 8

References 57

[1]	曹建华, 林位夫, 陈俊明. 砧木与接穗嫁接亲合力研究综述[J]. 热带农业科学, 2005, 25(4): 64-69.
	CAO J H, LIN W F, CHEN J M. Studies of affinity between rootstock and scion[J]. Chinese Journal of Tropical Agriculture, 2005, 25(4): 64-69. (in Chinese with English abstract)
[2]	杨蕾, 李勋兰, 杨海健, 等. 重庆市柑橘产业发展成就、存在问题与建议[J]. 南方园艺, 2020, 31(3): 32-35.
	YANG L, LI X L, YANG H J, et al. The developing achievement, existing problems and suggestions of citrus industry in Chongqing[J]. Southern Horticulture, 2020, 31(3): 32-35. (in Chinese with English abstract)
[3]	郭玲霞, 陈鹏, 孙元学, 等. 不同中间砧对金秋砂糖橘树体生长及果实品质的影响[J]. 江西农业大学学报, 2021, 43(6): 1278-1286.
	GUO L X, CHEN P, SUN Y X, et al. Effects of different interstocks on tree growth and fruit quality of Jinqiushatangju[J]. Acta Agriculturae Universitatis Jiangxiensis, 2021, 43(6): 1278-1286. (in Chinese with English abstract)
[4]	李伟佳, 王铁, 王均. 3种中间砧对‘春见’柑橘树体生长、产量和果实品质的影响[J]. 中国果树, 2020(1): 76-78.
	LI W J, WANG T, WANG J. Effects of three interstocks on growth, yield and fruit quality of ‘Harumi’ tangor[J]. China Fruits, 2020(1): 76-78. (in Chinese with English abstract)
[5]	杨晓婷. 不同中间砧以及套袋对‘爱媛28’果实品质的影响[D]. 武汉: 华中农业大学, 2022.
	YANG X T. Effect of different intermediate rootstocks and bagging on fruit quality of ‘Ehime 28’[D]. Wuhan: Huazhong Agricultural University, 2022. (in Chinese with English abstract)
[6]	王铁, 黄胜佳, 杨友婷, 等. 不同中间砧对媛小春柑橘生长与光合特性的影响[J]. 浙江农业学报, 2021, 33(3): 413-421.
	WANG T, HUANG S J, YANG Y T, et al. Effects of different interstocks on growth and photosynthetic characteristics of Yuanxiaochun citrus[J]. Acta Agriculturae Zhejiangensis, 2021, 33(3): 413-421. (in Chinese with English abstract)
[7]	叶宇, 欧春青, 王斐, 等. 梨矮化砧木致矮机制研究进展[J]. 果树学报, 2022, 39(11): 2163-2171.
	YE Y, OU C Q, WANG F, et al. Progress in research on the dwarfing mechanism of pear dwarfing rootstocks[J]. Journal of Fruit Science, 2022, 39(11): 2163-2171. (in Chinese with English abstract)
[8]	刘晓, 宋瑞琴, 刘永忠, 等. 2种中间砧对爱媛38号杂柑矿质元素含量及果实品质的影响[J]. 浙江农业科学, 2015, 56(4): 482-484.
	LIU X, SONG R Q, LIU Y Z, et al. Effects of two kinds of intermediate rootstocks on mineral element content and fruit quality of Ehime 38 hybrid orange[J]. Journal of Zhejiang Agricultural Sciences, 2015, 56(4): 482-484. (in Chinese)
[9]	杨瀚生. 不同基砧、中间砧组合对伦晚树体生长及果实品质的影响[D]. 武汉: 华中农业大学, 2022.
	YANG H S. Effects of different basal and intermediate stock combinations on tree growth and fruit quality of Lane late navel orange[D]. Wuhan: Huazhong Agricultural University, 2022. (in Chinese with English abstract)
[10]	陈招芳, 黎思辰, 杨镰聪, 等. 不同砧木对塔罗科血橙果实抗氧化能力的影响[J]. 云南农业大学学报(自然科学), 2022, 37(3): 447-454.
	CHEN Z F, LI S C, YANG L C, et al. Effects of different rootstock on the antioxidant ability of ‘Tarocco’ blood orange fruit[J]. Journal of Yunnan Agricultural University(Natural Science), 2022, 37(3): 447-454. (in Chinese with English abstract)
[11]	里程辉, 王杰, 王宏, 等. 淹水胁迫下不同中间砧对岳华苹果叶片和根系抗氧化酶、非酶类抗氧化物活性的影响[J]. 江苏农业科学, 2022, 50(11): 130-135.
	LI C H, WANG J, WANG H, et al. Impacts of different interstocks on activities of antioxidant enzymes and non-enzymatic antioxidants in leaves and roots of Yuehua apple under waterlogging stress[J]. Jiangsu Agricultural Sciences, 2022, 50(11): 130-135. (in Chinese with English abstract)
[12]	周开兵, 夏仁学, 罗中奎. 3种中间砧对纽荷尔脐橙树体生长和若干生理生化指标的影响[J]. 亚热带植物科学, 2003, 32(4): 4-7.
	ZHOU K B, XIA R X, LUO Z K. Influences of 3 kinds of interstock on tree growth of Newhall navel orange and some physiological and biochemical indexes in leaf and root[J]. Subtropical Plant Science, 2003, 32(4): 4-7. (in Chinese with English abstract)
[13]	陈启亮. 我国杂柑良种选育进展[J]. 中国南方果树, 2002, 31(4): 3-4.
	CHEN Q L. Advances in the breeding of citrus hybrids in China[J]. South China Fruits, 2002, 31(4): 3-4. (in Chinese)
[14]	江东, 曹立. 晚熟高糖杂柑品种“沃柑”在重庆的引种表现[J]. 中国南方果树, 2011, 40(5): 33-34.
	JIANG D, CAO L. Introduction performance of late-maturing high-sugar hybrid orange variety “Wogan” in Chongqing[J]. South China Fruits, 2011, 40(5): 33-34. (in Chinese)
[15]	陈竹生, 江东, 洪棋斌, 等. 春见桔橙主要性状和栽培技术[J]. 中国南方果树, 2004, 33(2): 5.
	CHEN Z S, JIANG D, HONG Q B, et al. Biological characteristics of Harumi tangor and its cultural technique[J]. South China Fruits, 2004, 33(2): 5. (in Chinese)
[16]	洪林, 付世军, 杨蕾, 等. 晚熟杂柑新品种“探戈”在重庆的引种表现[J]. 南方农业, 2018, 12(31): 56-58.
	HONG L, FU S J, YANG L, et al. Introduction of a new variety of late-ripening mixed mandarin “Tango” in Chongqing[J]. South China Agriculture, 2018, 12(31): 56-58. (in Chinese with English abstract)
[17]	夏仁斌, 熊静丹, 吴正亮, 等. 重庆三峡库区晚熟脐橙品种特性介绍[J]. 中国南方果树, 2009, 38(6): 10-11.
	XIA R B, XIONG J D, WU Z L, et al. Characteristics of late-maturing navel orange varieties in Chongqing Three Gorges Reservoir area[J]. South China Fruits, 2009, 38(6): 10-11. (in Chinese)
[18]	高俊凤. 植物生理学实验指导[M]. 北京: 高等教育出版社, 2006.
[19]	张志良, 瞿伟菁. 植物生理学实验指导[M]. 3版. 北京: 高等教育出版社, 2003.
[20]	赵通, 程丽, 王城, 等. 不同苹果砧穗组合的生长及光合特性[J]. 西北植物学报, 2018, 38(9): 1707-1716.
	ZHAO T, CHENG L, WANG C, et al. Effect of different apple scion-rootstock combinations on growth and photosynthesis characteristics[J]. Acta Botanica Boreali-Occidentalia Sinica, 2018, 38(9): 1707-1716. (in Chinese with English abstract)
[21]	阿布来克·尼牙孜, 章世奎, 樊国全, 等. 不同矮化中间砧木对库尔勒香梨光合特性的影响[J]. 新疆农业科学, 2020, 57(9): 1681-1688.
	ABULAIKE N, ZHANG S K, FAN G Q, et al. Effects of different dwarf interstocks on photosynthetic characteristics of Korla fragrant pear[J]. Xinjiang Agricultural Sciences, 2020, 57(9): 1681-1688. (in Chinese with English abstract)
[22]	欧毅, 王进, 谢永红, 等. 不同砧木对李叶片光合效能和生理生化指标的影响[J]. 西南农业大学学报(自然科学版), 2006, 28(3): 428-431.
	OU Y, WANG J, XIE Y H, et al. Effects of four rootstocks for plum on its photosynthesis and physiological and biochemical traits[J]. Journal of Southwest Agricultural University, 2006, 28(3): 428-431. (in Chinese with English abstract)
[23]	周军永, 陆丽娟, 孙其宝, 等. 不同砧木对“醉金香”葡萄生长及果实品质的影响[J]. 安徽农业大学学报, 2015, 42(1): 130-133.
	ZHOU J Y, LU L J, SUN Q B, et al. Effects of different root stocks on the growth and fruit quality of ‘Zuijinxiang’ grape[J]. Journal of Anhui Agricultural University, 2015, 42(1): 130-133. (in Chinese with English abstract)
[24]	何满, 田洋, 喻莹, 等. 不同砧木对“爱媛28号”橘橙营养器官组织养分吸收利用的影响[J]. 西南大学学报(自然科学版), 2022, 44(11): 80-87.
	HE M, TIAN Y, YU Y, et al. Effect of different rootstocks on nutrient absorption and utilization of vegetative organs in ‘Ehime 28’[J]. Journal of Southwest University(Natural Science Edition), 2022, 44(11): 80-87. (in Chinese with English abstract)
[25]	王中英, 赵雨明, 解思敏, 等. M9矮化中间砧段对³²P运转的影响[J]. 园艺学报, 1991, 18(3): 221-225.
	WANG Z Y, ZHAO Y M, XIE S M, et al. Effect of M9 dwarfing interstock on transport of ³²P[J]. Acta Horticulturae Sinica, 1991, 18(3): 221-225. (in Chinese with English abstract)
[26]	周开兵, 夏仁学, 王贵元, 等. 3种不同柑桔中间砧在树体矿质营养含量上的双重效应: Ⅰ不同中间砧对纽荷尔脐橙叶片矿质营养含量年变化的影响[J]. 中国农学通报, 2004, 20(1): 178-181.
	ZHOU K B, XIA R X, WANG G Y, et al. Double effects of 3 kinds of interstock on the contents of mineral nutrient of tree: Ⅰ effects of different kinds of interstock on the annual changes in the contents of mineral nutrient in leaf of newhall navel orange (Citrus sinensis Osbeck)[J]. Chinese Agricultural Science Bulletin, 2004, 20(1): 178-181. (in Chinese with English abstract)
[27]	WU S W, LI M, ZHANG C M, et al. Effects of phosphorus on fruit soluble sugar and citric acid accumulations in citrus[J]. Plant Physiology and Biochemistry, 2021, 160: 73-81.
[28]	WU K J, HU C X, LIAO P Y, et al. Potassium stimulates fruit sugar accumulation by increasing carbon flow in Citrus sinensis[J]. Horticulture Research, 2024, 11(11): uhae240.
[29]	HAN H, CHEN X L, LIU Y Z, et al. Foliar spraying magnesium promotes soluble sugar accumulation by inducing the activities of sucrose biosynthesis and transport in citrus fruits[J]. Scientia Horticulturae, 2024, 324: 112593.
[30]	SHAHID M A, BALAL R M, KHAN N, et al. Rootstocks influence the salt tolerance of Kinnow mandarin trees by altering the antioxidant defense system, osmolyte concentration, and toxic ion accumulation[J]. Scientia Horticulturae, 2019, 250: 1-11.
[31]	邱洁雅, 袁梦, 朱攀攀, 等. 不同柑橘砧木对锰过量胁迫的耐受性及生理响应[J]. 植物营养与肥料学报, 2021, 27(1): 109-121.
	QIU J Y, YUAN M, ZHU P P, et al. Tolerance and physiological response of citrus rootstock cultivars to manganese toxicity[J]. Journal of Plant Nutrition and Fertilizers, 2021, 27(1): 109-121. (in Chinese with English abstract)
[32]	金天, 徐月美, 邝冠翎, 等. 缺硼胁迫对枳幼苗根系生长及线粒体功能的影响[J]. 园艺学报, 2024, 51(1): 121-132.
	JIN T, XU Y M, KUANG G L, et al. Effect of boron deficiency on the root growth and mitochondrial function of trifoliate orange seedlings[J]. Acta Horticulturae Sinica, 2024, 51(1): 121-132. (in Chinese with English abstract)
[33]	王敏, 袁梦, 朱攀攀, 等. 柑橘4种砧木幼苗对铜过量胁迫的生理响应与耐受性差异[J]. 园艺学报, 2020, 47(10): 1969-1981.
	WANG M, YUAN M, ZHU P P, et al. Physiological response and tolerance to copper toxicity of four citrus rootstock seedlings[J]. Acta Horticulturae Sinica, 2020, 47(10): 1969-1981. (in Chinese with English abstract)
[34]	胡亚平, 郭雁君, 吉前华, 等. 柑桔几丁质酶基因家族的结构、分类与进化分析[J]. 中国南方果树, 2022, 51(6): 16-21.
	HU Y P, GUO Y J, JI Q H, et al. Analysis of structure, classification and evolution of chitinase gene family in citrus[J]. South China Fruits, 2022, 51(6): 16-21. (in Chinese with English abstract)
[35]	GENTILE A, DENG Z, MALFA S L, et al. Enhanced resistance to Phoma tracheiphila and Botrytis cinerea in transgenic lemon plants expressing a Trichoderma harzianum chitinase gene[J]. Plant Breeding, 2007, 126(2): 146-151.
[36]	张秀芝, 郭江云, 王永章, 等. 不同砧木对富士苹果矿质元素含量和品质指标的影响[J]. 植物营养与肥料学报, 2014, 20(2): 414-420.
	ZHANG X Z, GUO J Y, WANG Y Z, et al. Effects of different rootstocks on mineral contents and fruit qualities of Fuji apple[J]. Journal of Plant Nutrition and Fertilizer, 2014, 20(2): 414-420. (in Chinese with English abstract)
[37]	霍强强. 不同砧穗组合对苹果生长、果实品质及产量的影响[D]. 杨凌: 西北农林科技大学, 2017.
	HUO Q Q. Effects of different stock-scion combinations on growth, fruit quality and yield of apple[D]. Yangling: Northwest A&F University, 2017. (in Chinese with English abstract)
[38]	CABRERA D, RODRÍGUEZ P, UBERTI A, et al. ‘Williams’ pear (Pyrus communis L.) productivity and fruit quality when grafted onto different rootstocks[J]. Acta Horticulturae, 2021(1303): 523-528.
[39]	LI M M, GUO Z J, JIA N, et al. Evaluation of eight rootstocks on the growth and berry quality of ‘Marselan’ grapevines[J]. Scientia Horticulturae, 2019, 248: 58-61.
[40]	李超, 白世践, 耿新丽, 等. 不同砧木对‘赤霞珠’葡萄生长发育的影响[J]. 果树学报, 2016, 33(10): 1241-1250.
	LI C, BAI S J, GENG X L, et al. Effects of rootstocks on growth and development of ‘Cabernet Sauvignon’ grape[J]. Journal of Fruit Science, 2016, 33(10): 1241-1250. (in Chinese with English abstract)
[41]	金仲鑫. 不同砧木对葡萄果实品质的影响及机理初探[D]. 泰安: 山东农业大学, 2017.
	JIN Z X. Modifications of grape berry quality as affected by the rootstocks and preliminary exploration on the underlying mechanism[D]. Tai’an: Shandong Agricultural University, 2017. (in Chinese with English abstract)
[42]	KÖSE B, KARABULUT B, CEYLAN K. Effect of rootstock on grafted grapevine quality[J]. European Journal of Horticultural Science, 2014: 197-202.
[43]	淳长品, 彭良志, 雷霆, 等. 不同柑橘砧木对锦橙果实品质的影响[J]. 园艺学报, 2010, 37(6): 991-996.
	CHUN C P, PENG L Z, LEI T, et al. Effects of rootstocks on fruit quality of ‘Jincheng’ sweet orange[J]. Acta Horticulturae Sinica, 2010, 37(6): 991-996. (in Chinese with English abstract)
[44]	刘翔宇, 李娟, 黄敏, 等. 柑橘砧木对砂糖橘果实糖积累的影响[J]. 中国农业科学, 2015, 48(11): 2217-2228.
	LIU X Y, LI J, HUANG M, et al. Research on influences of rootstock on sugar accumulation in ‘Shatangju’ tangerine fruits[J]. Scientia Agricultura Sinica, 2015, 48(11): 2217-2228. (in Chinese with English abstract)
[45]	ALFARO J M, BERMEJO A, NAVARRO P, et al. Effect of rootstock on citrus fruit quality: a review[J]. Food Reviews International, 2023, 39(5): 2835-2853.
[46]	林媚, 姚周麟, 王天玉, 等. 8个杂交柑橘品种的糖酸组分含量及特征研究[J]. 果树学报, 2021, 38(2): 202-211.
	LIN M, YAO Z L, WANG T Y, et al. A study on the components and characteristics of sugars and acids in 8 hybrid citrus cultivars[J]. Journal of Fruit Science, 2021, 38(2): 202-211. (in Chinese with English abstract)
[47]	张阳, 张雅, 李娜, 等. 血橙果实品质综合评价分析系统开发与应用[J]. 果树学报, 2022, 39(2): 302-310.
	ZHANG Y, ZHANG Y, LI N, et al. Development and application of comprehensive evaluation system for blood orange fruit quality[J]. Journal of Fruit Science, 2022, 39(2): 302-310. (in Chinese with English abstract)
[48]	宋江涛, 谌丹丹, 公旭晨, 等. 人工蔬果对‘爱媛28’橘橙果实糖酸含量及代谢基因表达的影响[J]. 中国农业科学, 2022, 55(23): 4688-4701.
	SONG J T, SHEN D D, GONG X C, et al. Effects of artificial fruit thinning on sugar and acid content and expression of metabolism-related genes in fruit of Beni-Madonna tangor[J]. Scientia Agricultura Sinica, 2022, 55(23): 4688-4701. (in Chinese with English abstract)
[49]	彭杨, 马晓燕, 冯天乐, 等. 基于株高、产量、品质联合分析筛选砂糖橘优良砧木[J]. 果树学报, 2024, 41(6): 1078-1093.
	PENG Y, MA X Y, FENG T L, et al. Selection of excellent rootstocks for Shatang mandarin based on combined analysis of plant height, yield, and quality[J]. Journal of Fruit Science, 2024, 41(6): 1078-1093. (in Chinese with English abstract)
[50]	樊娟, 孙鲁龙, 刘振中, 等. 不同矮化中间砧对瑞雪苹果果实品质的影响[J]. 果树学报, 2023, 40(4): 680-689.
	FAN J, SUN L L, LIU Z Z, et al. Effects of different dwarfing interstocks on fruit quality of Ruixue apple[J]. Journal of Fruit Science, 2023, 40(4): 680-689. (in Chinese with English abstract)
[51]	朱志东, 吴韶辉, 徐建国. 甜桔柚在5种中间砧上的表现比较[J]. 中国南方果树, 2015, 44(4): 25-27.
	ZHU Z D, WU S H, XU J G. Performance comparison of sweet orange pomelo on five kinds of intermediate anvil[J]. South China Fruits, 2015, 44(4): 25-27. (in Chinese)
[52]	何文, 郭道祥, 王强, 等. 柑橘砧木‘蜀砧1号’对‘春见’生长和果实品质的影响[J]. 四川农业大学学报, 2024, 42(6): 1195-1202.
	HE W, GUO D X, WANG Q, et al. Effects of Citrus junos cv. ‘Shuzhen No.1’ on tree growth and fruit quality of ‘Harumi’[J]. Journal of Sichuan Agricultural University, 2024, 42(6): 1195-1202. (in Chinese with English abstract)
[53]	傅隆生, 宋思哲, 邵玉玲, 等. 基于主成分分析和聚类分析的海沃德猕猴桃品质指标综合评价[J]. 食品科学, 2014, 35(19): 6-10.
	FU L S, SONG S Z, SHAO Y L, et al. Comprehensive evaluation of kiwifruit quality based on principal component and cluster analysis[J]. Food Science, 2014, 35(19): 6-10. (in Chinese with English abstract)
[54]	朱世平, 王福生, 陈娇, 等. 柑橘不同类型砧木的种子和苗期性状[J]. 中国农业科学, 2020, 53(3): 585-599.
	ZHU S P, WANG F S, CHEN J, et al. Seed traits and seedling performances of different types of citrus rootstock[J]. Scientia Agricultura Sinica, 2020, 53(3): 585-599. (in Chinese with English abstract)
[55]	钟灶发, 张利娟, 高思思, 等. 干旱胁迫下4种柑橘砧木叶片细胞学特征及抗旱性比较[J]. 园艺学报, 2021, 48(8): 1579-1588.
	ZHONG Z F, ZHANG L J, GAO S S, et al. Leaf cytological characteristics and resistance comparison of four citrus rootstocks under drought stress[J]. Acta Horticulturae Sinica, 2021, 48(8): 1579-1588. (in Chinese with English abstract)
[56]	李勋兰, 洪林, 杨蕾, 等. 11个柑橘品种果实营养成分分析与品质综合评价[J]. 食品科学, 2020, 41(8): 228-233.
	LI X L, HONG L, YANG L, et al. Analysis of nutritional components and comprehensive quality evaluation of citrus fruit from eleven varieties[J]. Food Science, 2020, 41(8): 228-233. (in Chinese with English abstract)
[57]	徐宸宇, 唐启正, 刘慧宇, 等. 基于主成分分析综合评价6个杂交授粉组合的马家柚果实品质[J]. 果树学报, 2024, 41(2): 282-293.
	XU C Y, TANG Q Z, LIU H Y, et al. Comprehensive evaluation on fruit quality of six hybrid pollination combinations of Majiayou based on the principal component analysis[J]. Journal of Fruit Science, 2024, 41(2): 282-293. (in Chinese with English abstract)

编号 No.	含量 Content/(mg·g^-1)		活性 Activity
编号 No.	总酚 Total phenols	可溶性蛋白质 Soluble protein	SOD/ (U·g^-1)	CAT/(μmoL· min^-1· g^-1)	POD/ (U·g^-1)	PAL/ (U·g^-1)	β-1,3-GA/ (μg·min^-1· g^-1)	外切几丁质酶 Exochitinase ctivity/(μg· h^-1·g^-1)	内切几丁质酶 Endochitinase activity/(μg· h^-1·g^-1)	PPO/ (U· min^-1· g^-1)
LT	3.40 ±0.06	37.78 ±1.84	8 224.67 ±148.50	565.58 ±14.49^**	1 098.60 ±105.64	76.75 ±3.40^**	747.47 ±33.58^*	473.80 ±11.62^**	1 218.17 ±83.91^**	222.75 ±7.14
LAT	3.46 ±0.18	36.68 ±1.92	8 239.98 ±160.53	634.69 ±12.55^**	1 035.05 ±123.69	56.54 ±2.25^**	600.76 ±40.09^*	150.60 ±8.81^**	380.94 ±15.05^**	212.77 ±6.38
LO	3.35 ±0.05	32.51 ±2.13	6 523.70 ±174.36^**	425.71 ±15.82^*	1 016.24 ±85.08^*	10.68 ±0.56^*	615.64 ±22.02^*	180.42 ±15.16^**	485.61 ±10.93^**	204.10 ±11.86
LAO	3.58 ±0.04	33.71 ±2.29	5 165.95 ±142.08^**	383.03 ±11.79^*	796.43 ±22.84^*	8.06 ±0.59^*	539.27 ±26.49^*	474.02 ±15.41^**	1 220.63 ±160.55^**	202.47 ±11.99
LC	3.76 ±0.36	37.55 ±2.00	9 943.71 ±139.96	594.57 ±21.43	1 345.11 ±69.03	64.88 ±3.32	312.08 ±14.85	305.54 ±13.44	771.21 ±50.30^*	197.94 ±8.44
LAC	4.23 ±0.20	37.69 ±1.88	6 974.50 ±4 206.69	647.08 ±20.86	1 470.67 ±140.65	63.25 ±3.06	348.82 ±17.55	354.43 ±20.91	904.37 ±27.65^*	204.43 ±12.37

编号 No.	含量 Content/(mg·g^-1)		活性 Activity
编号 No.	总酚 Total phenols	可溶性蛋白质 Soluble protein	SOD/ (U·g^-1)	CAT/(μmoL· min^-1· g^-1)	POD/ (U·g^-1)	PAL/ (U·g^-1)	β-1,3-GA/ (μg·min^-1· g^-1)	外切几丁质酶 Exochitinase ctivity/(μg· h^-1·g^-1)	内切几丁质酶 Endochitinase activity/(μg· h^-1·g^-1)	PPO/ (U· min^-1· g^-1)
LT	3.40 ±0.06	37.78 ±1.84	8 224.67 ±148.50	565.58 ±14.49^**	1 098.60 ±105.64	76.75 ±3.40^**	747.47 ±33.58^*	473.80 ±11.62^**	1 218.17 ±83.91^**	222.75 ±7.14
LAT	3.46 ±0.18	36.68 ±1.92	8 239.98 ±160.53	634.69 ±12.55^**	1 035.05 ±123.69	56.54 ±2.25^**	600.76 ±40.09^*	150.60 ±8.81^**	380.94 ±15.05^**	212.77 ±6.38
LO	3.35 ±0.05	32.51 ±2.13	6 523.70 ±174.36^**	425.71 ±15.82^*	1 016.24 ±85.08^*	10.68 ±0.56^*	615.64 ±22.02^*	180.42 ±15.16^**	485.61 ±10.93^**	204.10 ±11.86
LAO	3.58 ±0.04	33.71 ±2.29	5 165.95 ±142.08^**	383.03 ±11.79^*	796.43 ±22.84^*	8.06 ±0.59^*	539.27 ±26.49^*	474.02 ±15.41^**	1 220.63 ±160.55^**	202.47 ±11.99
LC	3.76 ±0.36	37.55 ±2.00	9 943.71 ±139.96	594.57 ±21.43	1 345.11 ±69.03	64.88 ±3.32	312.08 ±14.85	305.54 ±13.44	771.21 ±50.30^*	197.94 ±8.44
LAC	4.23 ±0.20	37.69 ±1.88	6 974.50 ±4 206.69	647.08 ±20.86	1 470.67 ±140.65	63.25 ±3.06	348.82 ±17.55	354.43 ±20.91	904.37 ±27.65^*	204.43 ±12.37

编号 No.	矿质元素含量Mineral element content
编号 No.	N/%	P/%	K/%	Ca/(g· kg^-1)	Mg/(g· kg^-1)	Cu/(mg· kg^-1)	Zn/(mg· kg^-1)	Fe/(mg· kg^-1)	Mn/(mg· kg^-1)	B/(mg· kg^-1)
LT	2.91± 0.05	0.13± 0.01	1.02± 0.02	7.14± 0.04^**	6.27± 0.05	1.81± 0.06^**	6.70± 0.09	40.71± 1.00^*	85.38± 1.67^**	147.56± 6.71^*
LAT	2.79± 0.08	0.12± 0.01	0.98± 0.04	7.80± 0.07^**	6.29± 0.07	2.79± 0.07^**	6.52± 0.09	44.68± 0.94^*	55.34± 1.38^**	132.76± 2.97^*
LO	2.71± 0.07	0.12± 0.01*	1.01± 0.03^**	8.72± 0.53	7.08± 0.07	2.66± 0.07	6.95± 0.08^**	53.21± 4.52^*	45.86± 1.67	83.48± 2.74
LAO	2.94± 0.21	0.14± 0.01^*	1.14± 0.01^**	8.16± 0.07	6.95± 0.06	2.52± 0.08	7.27± 0.07^**	66.13± 2.72^*	45.21± 1.74	84.35± 3.45
LC	2.67± 0.07^**	0.12± 0.01	1.02± 0.03^*	8.62± 0.10^**	7.26± 0.10	2.93± 0.07	5.27± 0.07^**	53.29± 2.14^*	42.76± 2.12	83.68± 2.71^*
LAC	3.19± 0.10^**	0.14± 0.01	1.14± 0.03^*	7.79± 0.06^**	7.15± 0.07	2.97± 0.07	8.86± 0.53^**	47.70± 0.51^*	46.27± 0.84	90.72± 1.79^*

编号 No.	矿质元素含量Mineral element content
编号 No.	N/%	P/%	K/%	Ca/(g· kg^-1)	Mg/(g· kg^-1)	Cu/(mg· kg^-1)	Zn/(mg· kg^-1)	Fe/(mg· kg^-1)	Mn/(mg· kg^-1)	B/(mg· kg^-1)
LT	2.91± 0.05	0.13± 0.01	1.02± 0.02	7.14± 0.04^**	6.27± 0.05	1.81± 0.06^**	6.70± 0.09	40.71± 1.00^*	85.38± 1.67^**	147.56± 6.71^*
LAT	2.79± 0.08	0.12± 0.01	0.98± 0.04	7.80± 0.07^**	6.29± 0.07	2.79± 0.07^**	6.52± 0.09	44.68± 0.94^*	55.34± 1.38^**	132.76± 2.97^*
LO	2.71± 0.07	0.12± 0.01*	1.01± 0.03^**	8.72± 0.53	7.08± 0.07	2.66± 0.07	6.95± 0.08^**	53.21± 4.52^*	45.86± 1.67	83.48± 2.74
LAO	2.94± 0.21	0.14± 0.01^*	1.14± 0.01^**	8.16± 0.07	6.95± 0.06	2.52± 0.08	7.27± 0.07^**	66.13± 2.72^*	45.21± 1.74	84.35± 3.45
LC	2.67± 0.07^**	0.12± 0.01	1.02± 0.03^*	8.62± 0.10^**	7.26± 0.10	2.93± 0.07	5.27± 0.07^**	53.29± 2.14^*	42.76± 2.12	83.68± 2.71^*
LAC	3.19± 0.10^**	0.14± 0.01	1.14± 0.03^*	7.79± 0.06^**	7.15± 0.07	2.97± 0.07	8.86± 0.53^**	47.70± 0.51^*	46.27± 0.84	90.72± 1.79^*

处理 Treatment	单果重 Single fruit weight/g	单果纵径 Longitudinal diameter/mm	单果横径 Transversal diameter/mm	果形指数 Fruit shape index	可溶性固形物含量 Soluble solids content/%	可滴定酸含量 Titratable acid content/%	固酸比 TSS/TA	维生素C含量 Vitamin C content/ (mg·L^-1)
LT	95.31±5.31	59.82	66.06	0.91	13.5^*	0.85^**	15.88	240.6±0.9
LAT	100.13±4.53	60.43	67.43	0.90	12.9^*	0.63^**	20.48	231.5±16.4
LO	119.27±9.85	62.57	67.13	0.93	14.1	0.65	21.69	324.8±31.1
LAO	112.94±7.88	60.57	66.50	0.91	14.5	0.61	23.77	309.7±31.4
LC	153.65±5.11	70.31	71.54	0.98	12.8^*	0.97^**	13.19	421.0±23.6
LAC	143.49±8.20	70.11	70.53	0.99	11.9^*	0.76^**	15.66	354.9±15.1

Effects of interstock on leaf photosynthetic characteristics, physicochemical properties and fruit quality of three mandarin hybrids

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 8

References 57

Related Articles 15

Recommended Articles

Metrics

Comments

指标 Trait	载荷Load
指标 Trait	主成分1 PC1	主成分2 PC2	主成分3 PC3	主成分4 PC4
总叶绿素含量Total chlorophyll content	-0.59	-0.28	0.02	0.63
净光合速率Net photosynthetic rate	-0.99	0.14	0.05	-0.02
可溶性糖含量Soluble sugar content	0.92	0.21	0.31	-0.13
淀粉含量Starch content	-0.31	-0.55	0.23	-0.72
SOD活性SOD activity	0.43	-0.78	-0.43	0.05
CAT活性CAT activity	0.53	-0.78	0.27	-0.22
POD活性POD activity	0.87	-0.38	0.18	0.17
PAL活性PAL activity	0.37	-0.87	0.23	0.16
N	0.17	0.13	0.97	0.08
P	0.05	0.45	0.83	0.20
K	0.26	0.66	0.66	0.12
Ca	0.33	0.58	-0.70	-0.15
Mg	0.70	0.65	-0.21	0.16
Cu	0.71	0.28	-0.14	-0.60
Zn	0.06	0.33	0.86	-0.02
Fe	0	0.92	-0.20	-0.10
单果重Single fruit weight	0.95	0.26	-0.16	0.09
可溶性固形物含量Soluble solid content	-0.75	0.51	-0.35	0.18
可滴定酸含量Titratable acid content	0.58	-0.47	-0.24	0.55
维生素C含量Vitamin C content	0.83	0.39	-0.32	0.21
特征值Eigenvalue	7.34	5.67	4.20	1.90
贡献率Contribution rate/%	36.68	28.33	21.01	9.52
累计方差贡献率Cumulative contribution rate/%	36.68	65.01	86.02	95.53

处理 Treatment	F_AC1	F₁	F_AC2	F₂	F_AC3	F₃	F_AC4	F₄	综合得分 Comprehensive score	排名 Ranking
LT	-0.89	-2.42	-1.13	-2.68	0.43	0.89	1.34	1.85	-1.34	5
LAT	-0.52	-1.40	-0.95	-2.26	-0.06	-0.13	-1.73	-2.39	-1.47	6
LO	-0.47	-1.27	0.68	1.63	-0.91	-1.87	0.32	0.44	-0.37	4
LAO	-0.68	-1.83	1.50	3.58	0.33	0.67	-0.19	-0.26	0.48	3
LC	1.32	3.57	-0.26	-0.62	-1.28	-2.62	0.26	0.35	0.65	2
LAC	1.23	3.34	0.15	0.36	1.49	3.06	-0.01	-0.01	2.06	1

处理 Treatment	F_AC1	U₁	F_AC2	U₂	F_AC3	U₃	F_AC4	U₄	综合得分 Comprehensive score	排名 Ranking
LT	-0.89	0.01	-1.13	0.01	0.43	0.62	1.34	1.00	0.23	5
LAT	-0.52	0.17	-0.95	0.07	-0.06	0.44	-1.73	0.01	0.17	6
L O	-0.47	0.19	0.68	0.69	-0.91	0.13	0.32	0.67	0.36	4
LAO	-0.68	0.10	1.50	1.00	0.33	0.58	-0.19	0.50	0.49	3
LC	1.32	1.00	-0.26	0.33	-1.28	0.01	0.26	0.65	0.52	2
LAC	1.23	0.96	0.15	0.49	1.49	1.00	-0.01	0.56	0.75	1

[1]	ZHANG Shunchang, XU Jigen, FU Chengyue, PU Zhanxu, HU Lipeng, WU Hao, LI Junbing, XIN Liang, LEI Yuanjun. Effect of amino acid calcium spraying on peel cracking and quality of citrus hybrid Hongmeiren [J]. Acta Agriculturae Zhejiangensis, 2025, 37(8): 1706-1715.
[2]	WANG Chengyang, LIU Jieya, WU Minyi, XIE Boyi, HONG Decheng, LENG Feng, WU Guoquan. Effect of calcium treatment on the fruit quality of Reliance grape under waterlogging [J]. Acta Agriculturae Zhejiangensis, 2025, 37(7): 1451-1458.
[3]	XIANG Ying, CONG Jianmin, PAN Danhong, TAO Yonggang. Comprehensive evaluation of the growth process of different tomato varieties under spring organic greenhouse planting [J]. Acta Agriculturae Zhejiangensis, 2025, 37(6): 1252-1261.
[4]	LIU Qihua, SUN Zhaowen, ZHENG Chongke. Effects of nitrogen management on absorption and allocation of microelements in above-ground parts of dry direct-sowing rice [J]. Acta Agriculturae Zhejiangensis, 2025, 37(5): 987-997.
[5]	LI Yancui, LI Fuqiang, ZHOU Bo. Effects of deficit irrigation at different growth stages on photosynthetic characteristics, yield and quality of Astragalus membranaceus var. mongholicus [J]. Acta Agriculturae Zhejiangensis, 2025, 37(4): 779-789.
[6]	WANG Li, CHEN Liming, WANG Pengfei, ZHANG Bin, MU Xiaopeng. Effects of organic fertilizer combined with bacterial fertilizer on fruit quality and soil properties of Cerasus humilis [J]. Acta Agriculturae Zhejiangensis, 2025, 37(4): 820-830.
[7]	QIN Yukun, CHEN Junying, WANG Yuping, ZHANG Lijuan. Effects of reducing nitrogen and increasing carbon on cotton production and nitrogen absorption and utilization in the Yangtze River Basin of China [J]. Acta Agriculturae Zhejiangensis, 2025, 37(4): 869-879.
[8]	ZHU Lyuhan, FANG Kun, WU Xiaoli, YAO Qing, XIE Shuli, ZHENG Lei, AO Yue, CHEN Lihong, ZHAO Ke, WEI Jun, ZHANG Jin. Effect of microwave-assisted superheated steam heating on physicochemical property and sensory quality of pork batters [J]. Acta Agriculturae Zhejiangensis, 2025, 37(3): 679-688.
[9]	LIU Xun, XIA Qile, LI Yanpo, WANG Yangguang, LU Shengmin. Optimization of extraction process for soluble and insoluble dietary fibers from Ougan (Citrus suavissima Hort. ex Tanaka) pomace and the differences between their physicochemical properties and functional characteristics [J]. Acta Agriculturae Zhejiangensis, 2025, 37(1): 189-202.
[10]	SUN Li, ZHANG Shuwen, YU Zheping, ZHENG Xiliang, LIANG Senmiao, REN Haiying, QI Xingjiang. Effects of potassium humate on soil improvement, tree growth and fruiting of Chinese bayberry (Myrica rubra) [J]. Acta Agriculturae Zhejiangensis, 2024, 36(8): 1878-1886.
[11]	ZHU Xuehui, XIE Hui, HAN Shouan, WANG Min, BAI Shijian, MA Yunlong, WANG Yanmeng, MAI Sile, PAN Mingqi, ZHANG Wen. Effect of two plant growth regulators on the fruit quality of ‘Centennial Seedless’ grapes [J]. Acta Agriculturae Zhejiangensis, 2024, 36(6): 1309-1319.
[12]	WANG Ying, WANG Jian, FENG Zishan, WANG Baogen, WU Xinyi, LU Zhongfu, SUN Yuyan, DONG Wenqi, LI Guojing, WU Xiaohua. Factor analysis and comprehensive evaluation of the fruit quality of bottle gourd (Lagenaria siceraria) [J]. Acta Agriculturae Zhejiangensis, 2024, 36(2): 334-343.
[13]	LUO Shasha, WANG Ruyue, ZHEN Ziyi, WU Jialong, XU Yeyong, Bahetiyaer KERIM, SUN Yali, HU Haifang. Effect of irrigation time and amount on cracking rate and quality of apricot plum fruit [J]. Acta Agriculturae Zhejiangensis, 2024, 36(2): 365-372.
[14]	MA Ling, ZHANG Zhenwu, FANG Yingzi, WU Huixin, XING Chenghua. Effects of nitrogen reduction and biochar application on growth and development of Citurs reticulata Blanco cv. ‘Ponkan’ and soil properties [J]. Acta Agriculturae Zhejiangensis, 2024, 36(12): 2739-2747.
[15]	YUE Zongwei, LI Jiaxiao, SUN Xiangyang, LIU Guoliang, LI Suyan, WANG Chenchen, ZHA Guichao, WEI Ningxian. Effects of chemical fertilizer combined with organic fertilizer on soil properties, cherry fruit quality and yield [J]. Acta Agriculturae Zhejiangensis, 2023, 35(9): 2192-2201.