Abstract: The genetic diversity and relationship of 44 tomato accessions were analyzed by AFLP （amplified fragment length polymorphism）. Fifteen primer combinations selected from 64 primer combinations produced a total of 601 fragments，364 of which were polymorphic with a polymorphism percentage of 60.5%. This results showed that there were abundant diversities in enzyme digestion sites among different tomato varieties. These tomato germplasm resources were clustered into 5 multiple-member groups and 1 one-member group by UPGMA （unweighted pair group method with arithmetic average） based on Nei’s genetic distances. The genetic relationship of 44 tomato accessions was analyzed using the different coefficient.

Key words: tomato, AFLP, genetic diversity, relationship