Genetic diversity of Lingkun chicken based on microsatellite markers

›› 2010, Vol. 22 ›› Issue (4): 0-463.

• 论文 •

Genetic diversity of Lingkun chicken based on microsatellite markers

YUAN Qing-yan;YU De-bing;WU Chun-qin;LI Guo-qin;WANG De-qian;LU Li-zhi;*

1Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China; 2 College of Animal Science & Technique, Nanjing Agriculture University, Nanjing 210095, China; 3 Institute of Animal Science, Wenzhou Academy of Agricultural Sciences, Wenzhou 325006, China)

Received:1900-01-01 Revised:1900-01-01 Online:2010-07-25 Published:2010-07-25

Abstract

Abstract: In order to assess Lingkun chicken's genetic variation and population structure, 23 chicken-specific microsatellite loci were used to analyze the alleles for 30 individuals from natural population using PCR and polyacrylamide gel electrophoresis. A total of 174 alleles were detected across the 23 microsatellite loci. All the loci were polymorphic and the number of alleles varied from 3 to 12, and the mean number was 7.5 alleles per locus. The average heterozygosity and polymorphism information content of 23 microsatellite loci were 0.758 and 0.723, respectively. The results indicated that there was high genetic diversity in the natural population of Lingkun chicken．

Key words: microsatellite marker, Lingkun chicken, genetic diversity, heterozygosity, polymorphism information content

YUAN Qing-yan;YU De-bing;WU Chun-qin;LI Guo-qin;WANG De-qian;LU Li-zhi;*. Genetic diversity of Lingkun chicken based on microsatellite markers[J]. , 2010, 22(4): 0-463.

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Genetic diversity of Lingkun chicken based on microsatellite markers

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