Effect of antibiotic stress on antibiotic resistance genes in soils with different fertilization histories

doi:10.3969/j.issn.1004-1524.20250024

Acta Agriculturae Zhejiangensis ›› 2026, Vol. 38 ›› Issue (3): 551-558.DOI: 10.3969/j.issn.1004-1524.20250024

• Environmental Science • Previous Articles Next Articles

Effect of antibiotic stress on antibiotic resistance genes in soils with different fertilization histories

ZHU Sengen¹(), CHENG Jianhua²^,^*(), ZHU Xingna³, LI Jianxin⁴, TANG Xiangyu²

^1. Zhejiang Honggaitou Agricultural Science and Technology Co., Ltd., Jiangshan 324109, Zhejiang, China
^2. School of Forestry and Biotechnology, Zhejiang A&F University, Hangzhou 311300, China
^3. School of Modern Agriculture, Jiaxing Vocational & Technical College, Jiaxing 314036, Zhejiang, China
^4. Forestry Bureau of Jiangshan County, Jiangshan 324100, Zhejiang, China

Received:2025-01-06 Online:2026-03-25 Published:2026-04-17
Contact: CHENG Jianhua

Abstract

Abstract:

The effects of antibiotic selective pressure on antibiotic resistance genes (ARGs) in agricultural soils with long term application of different manures remain poorly understood. The high-throughput quantitative polymerase chain reaction technology was adopted in the present study to analyze the effect of antibiotic dose on the number, relative abundance and composition of ARGs in farmland soils subjected to long-term application of chemical fertilizers, chicken manure, and pig manure. The results showed that a total of 109 ARGs and 24 mobile genetic elements (MGEs) were detected across all soil samples. Genes conferring resistance to aminoglycosides and multiple drugs were the two most dominant types of ARGs. Long-term manure application enhanced both the number and relative abundance of soil ARGs and altered their distribution patterns, with pig manure demonstrating a more pronounced effect. Likely, antibiotic addition also stimulated the spread of ARGs in soil, while its extent increased with antibiotic concentration and varied depending on the soil fertilization history. The response of ARGs detection frequency to antibiotic stress was more pronounced in soils with a history of chemical fertilizer application, while the relative concentration and distribution patterns of ARGs in manure-amended soils showed greater sensitivity to antibiotics. Additionally, the relative abundance of soil MGEs showed a significantly (p<0.01) positive correlation with that of ARGs irrespective of soil types. These results provided a scientific basis for the evaluation of health risk of agricultural application of livestock manure.

Key words: soil, fertilization history, antibiotic stress, antibiotic resistance genes, dose-response

CLC Number:

ZHU Sengen, CHENG Jianhua, ZHU Xingna, LI Jianxin, TANG Xiangyu. Effect of antibiotic stress on antibiotic resistance genes in soils with different fertilization histories[J]. Acta Agriculturae Zhejiangensis, 2026, 38(3): 551-558.

Figures/Tables 7

Table 1 Basic physicochemical properties and antibiotic residue content of the original soil sample

样本 Sample	pH值 pH value	有机碳含量/(mg·g^-1) Organic carbon content/(mg·g^-1)	总氮含量/(mg·g^-1) Total nitrogen content/(mg·g^-1)	抗生素残留量/(μg·kg^-1) Antibiotic residue/(μg·kg^-1)
样本 Sample	pH值 pH value	有机碳含量/(mg·g^-1) Organic carbon content/(mg·g^-1)	总氮含量/(mg·g^-1) Total nitrogen content/(mg·g^-1)	SD	SMT	SMZ	OTC	DOXC
CM	5.57	22.23	1.32	6.23	2.98	1.51	5.25	7.36
PM	6.45	24.08	1.42	10.68	19.57	3.44	28.91	16.26
NPK	4.58	13.40	1.29	0.18	—	0.28	1.38	0.14

Fig.1 The number of detected antibiotic resistance genes in soils with different antibiotic addition levels PM, CM, and NPK represent the soil of upland farmlands under long-term (more than 5 years) application of pig manure, chicken manure, and chemical fertilizers, respectively. The same as below. Bars marked without the same letters indicate significan difference at p<0.05. MLSB, Macrolide-lincosamide-streptogramin B.

Fig.2 Relative abundance of detected antibiotic resistance genes in soils with different antibiotic addition levels Bars marked without the same letters indicate significan difference at p<0.05 for the same sample.

Fig.3 Distribution pattern of antibiotic resistance genes in soil based on principal coordinate analysis

Fig.4 The number of detected mobile genetic elements in soils with different antibiotic addition levels

Fig.5 Relative abundance of detected mobile genetic elements in soils with different antibiotic addition levels

Fig.6 Correlation between the relative abundance of antibiotic resistance genes (ARGs) and mobile genetic elements (MGEs) in soil samples

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Effect of antibiotic stress on antibiotic resistance genes in soils with different fertilization histories

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