不同连作年限菊花根际土壤微生物多样性变化特征

doi:10.3969/j.issn.1004-1524.2022.12.14

浙江农业学报 ›› 2022, Vol. 34 ›› Issue (12): 2710-2719.DOI: 10.3969/j.issn.1004-1524.2022.12.14

不同连作年限菊花根际土壤微生物多样性变化特征

张晓波(), 于春雷, 张文洋, 闫烨, 阮芳()

辽宁省经济作物研究所,辽宁辽阳 111000

收稿日期:2022-03-16 出版日期:2022-12-25 发布日期:2022-12-26
通讯作者: 阮芳
作者简介:*阮芳,E-mail:yuanfang_renee@163.com
张晓波(1980—),女,黑龙江林甸人,硕士,副研究员,从事花卉资源创新、新品种选育与栽培技术研究。E-mail:zxb0459@163.cm
基金资助:
辽宁省科学技术计划(2020JH2/10200016);辽宁省农业科学院基本科研业务费专项(2021GR2910)

Variation characteristics of soil microbial diversity in chrysanthemum rhizosphere soil with different continuous cropping years

ZHANG Xiaobo(), YU Chunlei, ZHANG Wenyang, YAN Ye, RUAN Fang()

Liaoning Research Institute of Cash Crops, Liaoyang 111000, Liaoning, China

Received:2022-03-16 Online:2022-12-25 Published:2022-12-26
Contact: RUAN Fang

摘要/Abstract

摘要：

为揭示连作菊花根际土壤微生物群落结构和多样性变化特征,以不同连作年限菊花根际土壤为试验材料,采用高通量测序技术(Illumina-MiSeq)和生物信息学方法,分析无连作(CK)、连作2年(2a)、连作9年(9a)菊花根际土壤细菌和真菌的丰富度、多样性指数和群落结构,以期为菊花连作障碍防控提供理论支撑。结果表明,2a、9a根际土壤细菌数量和多样性较CK显著(P<0.05)增加,2a和9a的群落结构相似,共有OTU(operational taxonomic unit)比例高。连作对菊花根际土壤真菌群落多样性和丰度影响较小,但对真菌群落组成影响显著,2a、9a根际土壤真菌接合菌门、担子菌门和被孢霉属等群落丰度减少,明梭孢属、镰刀菌属、土赤壳菌等群落丰度增加。菊花连作会显著增加其根际土壤细菌数量和多样性,显著影响真菌群落结构组成。

关键词: 菊花, 连作, 根际土壤, 细菌, 真菌

Abstract:

In order to reveal the community structure and diversity characteristics of microbe in chrysanthemum rhizosphere soil under continuous cropping, taking the rhizosphere soil of chrysanthemum with different continuous cropping years as the research object, high-throughput sequencing technology (Illumina-MiSeq) and bioinformatics were used to analyze the bacterial and fungal community composition and diversity in chrysanthemum rhizosphere soil of no continuous cropping (CK), continuous cropping for 2-years (2a) and continuous cropping for 9 years (9a). The results showed that the number and diversity of bacteria in rhizosphere soil of chrysanthemum in 2a and 9a treatments were significantly (P<0.05) higher than those of CK. Continuous cropping had little influence on the fungal community diversity and abundance, but had significant influence on the fungal community composition. Zygomycota, Basidiomycota, Mortierella and other microbial communities were decreased, and the abundance of microbial communities such as Monographella, Fusarium, Ilyonectria and other microbial community were increased in 2a and 9a treatment, compared with CK. Continuous cropping of chrysanthemum significantly increased the number and diversity of bacteria, and significantly affected the composition of fungal community in the rhizosphere soil.

Key words: chrysanthemum, continuous cropping, rhizosphere soil, bacteria, fungal

中图分类号:

S154.3

张晓波, 于春雷, 张文洋, 闫烨, 阮芳. 不同连作年限菊花根际土壤微生物多样性变化特征[J]. 浙江农业学报, 2022, 34(12): 2710-2719.

ZHANG Xiaobo, YU Chunlei, ZHANG Wenyang, YAN Ye, RUAN Fang. Variation characteristics of soil microbial diversity in chrysanthemum rhizosphere soil with different continuous cropping years[J]. Acta Agriculturae Zhejiangensis, 2022, 34(12): 2710-2719.

图/表 6

图1 不同连作年限菊花根际土壤细菌(A)和真菌(B)群落维恩图

Fig.1 Venn diagram of bacteria (A) and fungal (B) communities in chrysanthemum rhizosphere soil under different continuous cropping years

表1 不同连作年限菊花根际土壤细菌和真菌群落丰富度与多样性指数

Table 1 Richness and diversity indices of bacteria and fungal communities in chrysanthemum rhizosphere soil under different continuous cropping years

微生物类群 Microbial groups	处理 Treatment	覆盖度 Coverage	Ace指数 Ace index	Chao指数 Chao index	Shannon指数 Shannon index	Simpson指数 Simpson index
细菌Bacteria	CK	0.992 0	4 691.305 2 b	4 645.523 2 b	6.640 4 b	0.005 2 a
	2a	0.993 4	5 553.566 7 a	5 465.612 0 a	6.953 1 a	0.004 2 a
	9a	0.991 8	5 270.647 6 a	5 177.709 1 a	6.742 5 b	0.004 7 a
真菌Fungi	CK	0.999 6	729.559 3 a	728.330 5 a	3.125 7 a	0.118 7 a
	2a	0.999 8	848.267 0 a	846.308 7 a	3.746 6 a	0.064 0 a
	9a	0.999 7	728.287 6 a	729.722 2 a	3.193 0 a	0.145 0 a

图2 不同连作年限菊花根际土壤细菌(A)和真菌(B)属水平群落热图

Fig.2 Heatmap diagram of bacteria (A) and fungal (B) communitiesin chrysanthemum rhizosphere soil under different continuous cropping years at genus level

图3 不同连作年限菊花根际土壤细菌(A)和真菌(B)PCoA分析

Fig.3 Principal coordinate analysis of bacteria (A) and fungal (B) in chrysanthemum rhizosphere soil under different continuous cropping years

图4 不同连作年限菊花根际土壤细菌(A)和真菌(B)群落门水平组成与相对丰度 A:Proteobacteria,变形菌门;Acidobacteria,酸杆菌门;Actinobacteria,放线菌门;Bacteroidetes,拟杆菌门;Chloroflexi,绿弯菌门;Gemmatimonadetes,芽单胞菌门;Saccharibacteria,糖杆菌门;Firmicutes,厚壁菌门;Nitrospirae,硝化螺旋菌门;Verrucomicrobia,疣微菌门;Planctomycetes,扁平菌纲;Others,其他。 B:Ascomycota,子囊菌门;Fungi_unclassified,真菌_未分类;Chytridiomycota,壶菌门;Zygomycota,接合菌门;Basidiomycota,担子菌门;Glomeromycota,球囊菌门;Others,其他。

Fig.4 Composition and relative abundance of bacteria (A) and fungal (B) in chrysanthemum rhizosphere soil under different continuous cropping years at phylum level

图5 不同连作年限菊花根际土壤细菌(A)和真菌(B)群落属水平组成与相对丰度 A:Acidobacteria_noran,k酸杆菌属;Anaerolineaceae_uncultured,厌氧绳菌属_未培养;Flavobacterium,黄杆菌属;Saccharibacteria_norank,糖杆菌属;Nitrosomonadaceae_uncultured,亚硝化单胞菌属_未培养;Gemmatimonadaceae_uncultured,芽单胞菌属_未培养;Micrococcaceae_uncultured,微球菌属;Sphingomonas,鞘氨醇单胞菌属;Bacillus,芽孢杆菌;Nitrospira,硝化螺菌;Devosia,德沃斯氏菌属;Ramlibacter,拉姆利氏杆菌;Cytophagaceae_uncultured,噬纤维菌属_未培养;Rhizomicrobium,根霉菌属;Bryobacter,苔藓菌属;Acidimicrobiales_uncultured,酸微菌属_未培养;Comamonadaceae_unclassified,丛毛单胞菌属_未培养; Blastocatellaceae_Subgroup_4_uncultured,酸杆菌属_亚组_4_未培养;Acidobacteriaceae_Subgroup_1_uncultured,酸杆菌属_亚组_1_未培养;Actinobacteria_norank,放线菌属;Gemmatimonas,芽单胞菌属;Others,其他。 B:Fungi_unclassified,真菌_未分类;Olpidiaster_unclassified;Mortierella,被孢霉属;Monographella,明梭孢属;Paraphoma,异茎点霉属;Chaetomiaceae_unclassified,毛壳菌属_未分类;Gibellulopsis;Pyronemataceae_unclassified,火丝菌属_未分类;Chaetomium,毛壳菌属;Fusarium,镰刀菌属;Ilyonectria,土赤壳属;Ascomycota_unclassified,子囊菌_未分类;Funneliformis,管柄囊霉;Cladosporium,枝孢菌属;Alternaria,链格孢属;Pseudaleuria;Agaricomycetes_unclassified,伞菌纲_未分类;Dothideomycetes_unclassified,座囊菌纲_未分类;Entoloma,粉褶菌属;Arthrobotrys,节丛孢属;Sporidiobolales_fam_Incertae_sedis_unclassified,锁掷酵母目_未分类;Others,其他。

Fig.5 Composition and relative abundance of bacteria(A) and fungal(B) in chrysanthemum rhizosphere soil under different continuous cropping years at genus level

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不同连作年限菊花根际土壤微生物多样性变化特征

Variation characteristics of soil microbial diversity in chrysanthemum rhizosphere soil with different continuous cropping years

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