浙江农业学报

• 园艺科学 • 上一篇    下一篇

光质对试管生姜生长发育及微型姜形成、品质的影响

  

  1. (1潍坊科技学院 蔬菜花卉研究所,山东 寿光 262700;2作物生物学国家重点实验室,农业部园艺作物生物学实验室,山东农业大学 园艺科学与工程学院,山东 泰安 271018)
  • 出版日期:2015-07-25 发布日期:2015-08-03

Effect of light quality on the growth, micro\|rhizome induction and quality of in vitro ginger#br#


  1. (1 Institute of Vegetables and Flowers, Weifang University of Science and Technology, Shouguang 262700, China; 2 State Key Laboratory of Crop Biology/Ministry of Agriculture Key Laboratory of Horticultural Crop Biology/College of Horticulture Science and Engineering, Shandong Agricultural University, Taian 271018, China)
  • Online:2015-07-25 Published:2015-08-03

摘要: 为提高生姜微繁效率,研究了不同光质对生姜试管苗生长和微型姜形成及品质的影响。结果表明,与白光处理相比,绿光、红光均显著增加了生姜试管苗的株高,绿光显著降低了其繁殖系数,而蓝光则增加了其繁殖系数;黄光对试管苗生长的影响较小。生姜试管苗叶片净光合速率以绿光处理较低,蓝光和红光较高,但叶片气孔导度及蒸腾速率则以蓝光、黄光处理较高,红光、绿光处理较低。绿光、蓝光、红光均可诱导试管苗较早形成微型姜,而黄光则延迟了微型姜的形成;微型姜鲜重以绿光处理较高,较白光处理增加60%以上;但微型姜干物质、淀粉、粗纤维含量均以绿光处理较低,蓝光处理较高;可溶性糖含量以红光、黄光处理较高,绿光、蓝光处理较低,而可溶性蛋白含量基本呈相反的趋势。综合考虑,绿光有利于微型姜鲜重的增加,但蓝光更有利于提高组培快繁效率及微型姜的大量获得。

关键词: 光质, 生姜试管苗, 生长发育, 根状茎诱导, 微型姜品质

Abstract: In order to improve the efficiency of micropropagation of ginger, the effects of different light quality on the growth and micro\|rhizome induction of in vitro ginger were studied. The results showed that compared with white light, plant height of in vitro ginger increased under green and red lights. The propagation coefficients were significantly reduced under green light, but were just opposite under blue light, and yellow light had a little effect on the growth of in vitro ginger. The net photosynthetic rate of ginger leaves was the lowest under green light, but higher under blue and red light, and stomatal conductance and transpiration rate were higher under blue and yellow light, but lower under red and green light. The time of micro\|rhizome formation of in vitro ginger under green, blue and red lights were earlier than that under white light, but later under yellow light. Moreover, the micro\|rhizome fresh weight of in vitro ginger under green light was higher, which increased by over 60% compared with that under white light. Otherwise the dry mass content, starch and crude cellulose of micro\|rhizome under green light were the lowest, but the highest under blue light. Soluble sugar content was higher under red and yellow light, while lower under green and blue light, but contrary results were observed in soluble protein content. Taken together, green light helps to increase fresh weight of micro ginger, but blue light is more conducive to improve the efficiency of tissue culture and micro ginger in large quantities.

Key words: light quality, in vitro ginger, growth, rhizome induction, micro ginger quality