关键词: 铁皮石斛, 组织培养, 优化

Abstract:  The seed of Dendrobium candidum was used as test materials to compare the effects of seed disinfection methods， agar， organic compound and activated carbon on adventitious buds proliferation and shoots strengthening and roots induction in tissue culture，in order to optimize the preliminary system for the tissue culture of Dendrobium candidum. The results showed that the proliferation of protocorm\|like bodies was higher than multiple shoots. The disinfection method of 75% alcohol （for 30 s）+80% NaClO （for 8 min） was optimum for seeds，which could replace the traditional method of 01% HgCl2. The liquid medium was superior to semi liquid and solid culture medium. Addition of 10% banana juice and 05% activated carbon in culture medium could promote the growth of shoots and roots.

Key words: Dendrobium candidum, tissue culture, optimization