鸡TGF-β2基因启动子区多态性分析

doi:10.3969/j.issn.1004-1524.2016.12.10

浙江农业学报 ›› 2016, Vol. 28 ›› Issue (12): 2028-2032.DOI: 10.3969/j.issn.1004-1524.2016.12.10

鸡TGF-β2基因启动子区多态性分析

鲁伟, 吴允, 徐璐, 徐琪, 常国斌, 陈国宏^*

扬州大学动物科学与技术学院,江苏扬州 225009

收稿日期:2016-05-12 出版日期:2016-12-15 发布日期:2017-01-05
通讯作者: 陈国宏,E-mail: ghchen@yzu.edu.cn
作者简介:鲁伟(1990—),女,山东章丘人,硕士研究生,研究方向为动物遗传育种与繁殖。 E-mail: 18252775129@163.com
基金资助:
江苏省六大人才高峰项目资助 (2015-NY-019); 江苏省农业科技支撑(BE2013392)

Polymorphism analysis of TGF-β2 gene promoter of chickens

LU Wei, WU Yun, XU Lu, XU Qi, CHANG Guo-bin, CHEN Guo-hong^*

College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China

Received:2016-05-12 Online:2016-12-15 Published:2017-01-05

摘要/Abstract

摘要： 为了研究鸡TGF-β2基因启动子区的SNP及其对启动子功能元件的影响,采用PCR-SSCP方法对16个鸡种的TGF-β2启动子区的554~824 bp(即3号染色体的19 433 476~19 433 746 bp)进行基因分型,经测序,共检测到3个有效的SNPs位点,分别是679位点的C/G,699位点的G/A,774位点的T/C。其中大骨鸡和太湖鸡在3个位点均检测到SNP存在,AA鸡在3个位点都没有发现SNPs。分别利用生物信息学软件JASPAR和MethPrimer预测TGF-β2基因启动子区转录因子结合位点和CpG岛,发现SNP位点可改变转录因子结合位点且位于CpG岛上,表明TGF-β2启动子区SNPs可能通过不同方式影响基因表达调控。

关键词: 鸡, TGF-β2, 启动子, SNP

Abstract: To study the SNP in TGF-β2 gene promoter and its effect on the promoter functional elements, genotyping was used by PCR-SSCP in the region of 554-824 bp(19 433 476-19 433 746 bp in Chromosome 3) of the promoter of TGF-β2 gene among 16 chicken breeds. After the direct sequencing, we found three SNP loci totally in all breeds: the C/G in 679 loci, the G/A in 699 loci and the T/C in 774 loci. Three SNPs were detected in the Big-bone chicken and Taihu chicken, but no SNP was found in AA chicken. Moreover, we analyzed the transcription factor binding sites and CpG island by JASPAR and MethPrimer software respectively in TGF-β2 gene promoter, and found that SNPs changed the transcription factor binding sites and the CpG islands. The results suggested that SNPs in TGF-β2 promoter played an important role in the regulation of gene expression through different ways.

Key words: chicken, TGF-β2, promoter, SNP

中图分类号:

S831.2

鲁伟, 吴允, 徐璐, 徐琪, 常国斌, 陈国宏. 鸡TGF-β2基因启动子区多态性分析[J]. 浙江农业学报, 2016, 28(12): 2028-2032.

LU Wei, WU Yun, XU Lu, XU Qi, CHANG Guo-bin, CHEN Guo-hong. Polymorphism analysis of TGF-β2 gene promoter of chickens[J]. , 2016, 28(12): 2028-2032.

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鸡TGF-β2基因启动子区多态性分析

Polymorphism analysis of TGF-β2 gene promoter of chickens

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