药用真菌桑黄分子鉴定及遗传多样性分析

doi:10.3969/j.issn.1004-1524.2019.02.17

浙江农业学报 ›› 2019, Vol. 31 ›› Issue (2): 307-314.DOI: 10.3969/j.issn.1004-1524.2019.02.17

药用真菌桑黄分子鉴定及遗传多样性分析

王伟科¹, 宋吉玲¹, 巫优良², 闫静¹, 陆娜¹, 袁卫东¹, 陈观平³

1.杭州市农业科学研究院蔬菜研究所,浙江杭州 310024;
2.江山市农业科学研究所,浙江江山 324104;
3.浙江省中医药研究院,浙江杭州 310012

收稿日期:2018-05-09 出版日期:2019-02-25 发布日期:2019-03-06
作者简介:王伟科(1981—),男,浙江宁海人,硕士,高级农艺师,研究方向为食药用菌育种与栽培技术研究。 E-mail: akeok@126.com
基金资助:
浙江省科技计划(2016F10025); 杭州市农业与社会发展科研项目(20180416A02); 杭州市农业科学研究院科技创新项目(2017HNCX-03)

Molecular identification and genetic diversity of a traditional Chinese medicinal fungus Sanghuang

WANG Weike¹, SONG Jiling¹, WU Youliang², YAN Jing¹, LU Na¹, YUAN Weidong¹, CHEN Guanping³

1. Institute of Vegetables, Hangzhou Academy of Agricultural Sciences, Hangzhou 310024, China;
2. Jiangshan Institute of Agricultural Sciences, Jiangshan 324104, China;
3.Zhejiang Academy of Chinese Medicine, Hangzhou 310012, China

Received:2018-05-09 Online:2019-02-25 Published:2019-03-06

摘要/Abstract

摘要： 通过对不同来源的桑黄菌株进行分子鉴定和遗传多样性分析,为后续桑黄种质资源的研究、开发及利用提供参考。采用rDNA ITS序列分析技术,对收集到的国内22个桑黄菌株进行分子鉴定与遗传多样性分析。依据rDNA ITS序列计算遗传距离并构建系统发育树,结果显示收集到的桑黄菌株明确聚为3个独立类群,且3个类群的桑黄真菌存在明显的遗传分化。通过BLAST分析,成功鉴定出3类桑黄种质分别为:桑树桑黄(Sanghuangporus sanghuang)、杨树桑黄(Fuscoporia gilva)及丁香桑黄(Inonotus baumii)。不同来源的桑黄菌株间具有一定的遗传多样性,种间遗传趋异度显著高于种内。本研究结果提供了一种准确可靠且简单易行的桑黄真菌分子鉴定技术,可明确各桑黄真菌的种属来源及遗传结构组成,为桑黄真菌的进一步研究及开发应用奠定了基础。

关键词: 桑黄, rDNA ITS, 分子鉴定, 遗传多样性

Abstract: To study the genetic diversity and genetic relationship of Phellinus baumii, and their relative species by ITS molecular marker technique, and to provide the reference for Phellinus baumii identification and breeding, the ribosomal DNA internal transcribed spacer (rDNA ITS) region was amplified and sequenced to identify the 22 species of Sanghuang which were collected from domestic and detect its genetic diversity. Based on rDNA ITS sequences, the genetic distance and phylogenetic tree were obtained. Results showed that there were distinct genetic divergences among 22 species of Sanghuang, which formed three separate species cluster in the topology tree obviously with high bootstrap value. Meanwhile, two counterfeit Sanghuang strain, Fuscoporia gilva and Inonotus baumii, were detected successfully, and the certified Sanghuang germplasms were distinguished as Sanghuangporus sanghuang unambiguously by BLAST analysis. The results of ITS analysis revealed that Phellinus baumii had the plentiful genetic diversity and the genetic relationships were consistent with morphological characters of taxonomy. ITS method was efficient for identification of Phellinus baumii, which could provide a scientific basis for the resource collection and identification of the species in Phellinus baumii.

Key words: Sanghuang, ribosomal DNA internal transcribed spacer, molecular identification, genetic biodiversity analysis

中图分类号:

S567.9
R282

王伟科, 宋吉玲, 巫优良, 闫静, 陆娜, 袁卫东, 陈观平. 药用真菌桑黄分子鉴定及遗传多样性分析[J]. 浙江农业学报, 2019, 31(2): 307-314.

WANG Weike, SONG Jiling, WU Youliang, YAN Jing, LU Na, YUAN Weidong, CHEN Guanping. Molecular identification and genetic diversity of a traditional Chinese medicinal fungus Sanghuang[J]. , 2019, 31(2): 307-314.

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药用真菌桑黄分子鉴定及遗传多样性分析

Molecular identification and genetic diversity of a traditional Chinese medicinal fungus Sanghuang

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