大豆异黄酮干预肥胖大鼠肝氧化应激及炎症反应

doi:10.3969/j.issn.1004-1524.2022.05.08

浙江农业学报 ›› 2022, Vol. 34 ›› Issue (5): 942-948.DOI: 10.3969/j.issn.1004-1524.2022.05.08

大豆异黄酮干预肥胖大鼠肝氧化应激及炎症反应

熊昕宜(), 许泽玉, 何念佳, 何俊博, 陈正礼, 黄超, 刘文涛, 罗启慧()

四川农业大学动物医学院, 动物疾病模型实验室, 四川成都 611130

收稿日期:2020-11-16 出版日期:2022-05-25 发布日期:2022-06-06
通讯作者: 罗启慧
作者简介:* 罗启慧,E-mail: lqhbiology@163.com
熊昕宜(1999—),女,云南保山人,本科生。E-mail: 15528283216@163.com
基金资助:
大学生创新训练计划(201710626045);四川省科技厅应用基础研究(2019YJ0426)

Effects of soy isoflavones on oxidative stress and inflammatory response in liver of rats with food borne obesity

XIONG Xinyi(), XU Zeyu, HE Nianjia, HE Junbo, CHEN Zhengli, HUANG Chao, LIU Wentao, LUO Qihui()

Animal Disease Model Laboratory, School of Animal Medicine, Sichuan Agricultural University, Chengdu 611130, China

Received:2020-11-16 Online:2022-05-25 Published:2022-06-06
Contact: LUO Qihui

摘要/Abstract

摘要：

本研究旨在探究不同剂量大豆异黄酮(soy isoflavones,SIF)干预肥胖大鼠后肝的氧化应激及炎症反应情况。准备80只SD大鼠,普通饲料喂养16只SD大鼠做空白对照;高脂饲料喂养64只SD大鼠9周建立肥胖大鼠模型,将64只模型大鼠随机分成4组,每组16只,大豆异黄酮(0、150、300、450 mg·kg^-1)灌喂,每周定期测量体重,连续干预4周。取肝脏组织,苏木素-伊红(Hematoxylin-Eosin,HE)染色观察肝脏组织形态学结构;检测肝脏组织中超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)活性;荧光定量PCR方法检测肝脏组织中促炎因子白细胞介素-6(IL-6)、白细胞介素-1β(IL-1β)和肿瘤坏死因子-α(TNF-α)的表达。组织病理学发现,肥胖大鼠出现典型的肝细胞脂肪变性伴肝组织炎性损伤,肝SOD、CAT和GSH-Px水平降低,IL-6、IL-1β和TNF-α mRNA表达水平升高。大豆异黄酮干预后,各剂量组肝组织病理损伤得到改善,抗氧化因子活性显著提高并呈剂量效应,促炎因子表达水平显著下调。大豆异黄酮可逆转肥胖致肝组织病理损伤和提高肝脏抗氧化活性,下调肥胖相关促炎因子基因的表达,上述结果可为大豆异黄酮的应用开发提供参考。

关键词: 大豆异黄酮, 氧化应激, 炎症, 肥胖, 肝, 大鼠

Abstract:

To investigate the effects of different doses of soy isoflavones (SIF) on oxidative stress and inflammatory response of liver in food-induced obese rats, 80 SD rats were prepared and 16 SD rats fed with ordinary feed were set as blank control. SD rats were fed with high-fat diet for 9 weeks to establish obesity rat model. Then 64 obese rats were randomly divided into 4 groups, 16 rats per group. The rats were given by gavage different does of soy isoflavone (0,150,300,450 mg·kg^-1) for 4 weeks, weighed and recorded every week. Four weeks later, the rats were killed, and the liver tissue was separated. The pathological changes of liver were detected by Hematoxylin-Eosin (HE) staining. Liver superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) activities were detected by chemical colorimetry. Liver tumor necrosis factor-α(TNF-α), interleukin-1β(IL-1β)、interleukin-6 (IL-6) and tumor necrosis factor-α(TNF-α) were measured by qRT-PCR. It was found by histopathology that typical fatty degeneration of hepatocytes with inflammatory injury of liver tissue occurred in obese rats. The activities of SOD, CAT and GSH-Px in liver tissue were decreased, and the expression levels of IL-6、IL-1β and TNF-α were increased (P<0.05). After the intervention of soybean isoflavones, the pathological damage of liver tissue in each dose group was improved, the activity of antioxidant factors was significantly increased and showed a dose effect. Besides, the expression of pro-inflammatory factors was significantly down-regulated. Soy isoflavones can reverse obesity-induced liver tissue pathological damage and increase liver antioxidant activity, and down-regulate the expression of obesity-related pro-inflammatory factors. These results provide a reference for the application and development of soy isoflavones.

Key words: soy isoflavone, oxidative stress, inflammation, obesity, liver, rat

中图分类号:

S856

熊昕宜, 许泽玉, 何念佳, 何俊博, 陈正礼, 黄超, 刘文涛, 罗启慧. 大豆异黄酮干预肥胖大鼠肝氧化应激及炎症反应[J]. 浙江农业学报, 2022, 34(5): 942-948.

XIONG Xinyi, XU Zeyu, HE Nianjia, HE Junbo, CHEN Zhengli, HUANG Chao, LIU Wentao, LUO Qihui. Effects of soy isoflavones on oxidative stress and inflammatory response in liver of rats with food borne obesity[J]. Acta Agriculturae Zhejiangensis, 2022, 34(5): 942-948.

图/表 4

图1 高脂肥胖及大豆异黄酮干预大鼠体重及血脂水平变化 TG,甘油三酯;TC,总胆固醇;HDL,高密度脂蛋白;LDL,低密度酯蛋白。*,**分别表示不同处理组间差异达显著(P<0.05)或极显著(P<0.01)水平。下同。

Fig.1 effect of high-fat obesity and isoflavones on body weight and serum lipid level in rats TG, Triglyceride; TC, Total cholesterol; HDL, High density lipoprotein; LDL, Low density lipoprotein. *, ** represented the significant difference at P<0.05 or P<0.01 among different treatments.The same as below.

图2 大豆异黄酮干预肥胖大鼠肝脏组织形态学变化 A, 空白对照组;B,肥胖对照组;C,低剂量组;D,中剂量组;E,高剂量组。

Fig.2 Histomorphological changes of liver in obese rats treated with Isoflavones A, Blank control group; B, Obese control group; C, Low-dose group; D, Mid-dose group; E, High-dose group.

表1 大豆异黄酮干预改善了肥胖大鼠肝脏氧化应激

Table 1 Isoflavones intervention ameliorates hepatic oxidative stress in obese rats

组别Group	SOD/(U·mg^-1)	CAT/(U·mg^-1)	GSH-Px/U
C组Group C	176.08±12.14	100.13±9.80	2.776±0.294 1
F组Group F	128.98±11.54^**	68.76±9.63^*	2.137±0.246 2
H组Group H	232.87±17.42^##	142.85±14.74^##	4.647±0.688 0^##
M组Group M	190.67±14.94^##	104.99±27.56^##	3.062±0.351 7^#
L组Group L	153.88±22.06	99.68±12.39	2.423±0.512 2

图3 大豆异黄酮干预下调肥胖大鼠肝脏炎症反应

Fig.3 Down regulation of liver inflammation in obese rats by isoflavones intervention

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大豆异黄酮干预肥胖大鼠肝氧化应激及炎症反应

Effects of soy isoflavones on oxidative stress and inflammatory response in liver of rats with food borne obesity

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