外源褪黑素对金线兰有效成分含量及抗氧化酶活性的影响

doi:10.3969/j.issn.1004-1524.2023.01.07

浙江农业学报 ›› 2023, Vol. 35 ›› Issue (1): 58-66.DOI: 10.3969/j.issn.1004-1524.2023.01.07

外源褪黑素对金线兰有效成分含量及抗氧化酶活性的影响

何秀丽(), 王人民()

浙江大学农业与生物技术学院,种子科学与技术中心,浙江杭州 310058

收稿日期:2021-09-06 出版日期:2023-01-25 发布日期:2023-02-21
通讯作者: *王人民,E-mail:wangrm@zju.edu.cn
作者简介:何秀丽(1994—),女,安徽合肥人,硕士,研究方向为作物种质资源创新。E-mail:21916142@zju.edu.cn
基金资助:
浙江博爱教育基金

Effects of exogenous melatonin on active components content and antioxidase activity of Anoectochilus roxburghii

HE Xiuli(), WANG Renmin()

Seed Science and Technology Center, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China

Received:2021-09-06 Online:2023-01-25 Published:2023-02-21

摘要/Abstract

摘要：

金线兰[Anoectochilus roxburghii(Wall.)Lindl.]是一种珍稀的兰科开唇兰属多年生单子叶草本植物,具有重要的药用价值。褪黑素(MT)是一类胺类激素,具有调节机体昼夜规律和机体抗氧化等多种生理功能。试验分别采用5 μmol·L^-1、500 μmol·L^-1、5 mmol·L^-1褪黑素根灌处理,研究褪黑素对金线兰生长发育和有效成分含量的影响,结果表明,5 μmol·L^-1的褪黑素根灌技术处理能够显著增加总黄酮和总氨基酸的含量,分别增加了27.27%和21.79%,而500 μmol·L^-1和5 mmol·L^-1褪黑素显著增加了多糖的含量,分别增加了20.79%和23.88%。3种浓度褪黑素处理下,叶绿素总含量显著高于对照41.56%~55.84%。5 μmol·L^-1褪黑素处理下,叶片中超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性显著增加了23.72%、180.00%和33.65%。综上,褪黑素通过影响金线兰叶绿素含量、代谢物积累和抗氧化酶活性,从而增强金线兰的抗逆性,进而增强金线兰移栽后的生命力。

关键词: 金线兰, 褪黑素, 有效成分, 抗氧化酶, 活性

Abstract:

Anoectochilus roxburghii (Wall.) Lindl. is a rare perennial monocot herb in the family Orchidaceae, of important medicinal value. Melatonin (MT) is a class of amine hormones with various physiological functions such as regulating body day and night and antioxidant. In order to investigate the role of melatonin in the process of tissue culture seedling transplanting to the substrate, melatonin with 5 μmol·L^-1, 500 μmol·L^-1 and 5 mmol·L^-1 was used to study the effects of root irrigation on seedling growth and effective component content. The results showed that 5 μmol·L^-1 melatonin root irrigation treatment could significantly increase the total flavonoid by 27.27% and total amino acids content by 21.79%, whereas 500 μmol·L^-1 and 5 mmol·L^-1 melatonin significantly increased the polysaccharide content by 20.79% and 23.88%. Under the three concentrations of melatonin, the total chlorophyll content was 41.56%-55.84% significantly higher than that of the control. Under 5 μmol·L^-1 melatonin treatment, the activities of SOD, POD and CAT in leaves were significantly increased by 23.72%, 180.00% and 33.65%. In conclusion, melatonin could enhance the stress resistance of A.roxburghii by affecting chlorophyll content, metabolite accumulation and antioxidant enzyme activity, thus enhancing the vitality of A.roxburghii after transplanting.

Key words: Anoectochilus roxburghii, melatonin, active components, antioxidant enzyme, activity

中图分类号:

S682.31

何秀丽, 王人民. 外源褪黑素对金线兰有效成分含量及抗氧化酶活性的影响[J]. 浙江农业学报, 2023, 35(1): 58-66.

HE Xiuli, WANG Renmin. Effects of exogenous melatonin on active components content and antioxidase activity of Anoectochilus roxburghii[J]. Acta Agriculturae Zhejiangensis, 2023, 35(1): 58-66.

图/表 10

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处理	株高	茎粗	叶长	叶宽	根长	根粗	单株鲜重	单株干重	折干率
Treatment	Plant height/cm	Stem diameter/mm	Leaf length/cm	Leaf width/cm	Root length/cm	Root diameter/mm	Fresh weight per plant/g	Dry weight per plant/g	Drying rate/%
CK	4.99±0.28 a	2.99±0.20 a	3.38±0.02 bc	2.73±0.05 a	3.40±0.35 a	1.18±0.07 b	1.17±0.14 a	0.11±0.01 a	9.59±1.50 a
M1	5.05±0.28 a	3.01±0.22 a	3.48±0.04 ab	2.81±0.07 a	3.40±0.19 a	1.37±0.02 a	1.18±0.03 a	0.11±0.01 a	9.52±0.23 a
M2	5.07±0.10 a	3.32±0.19 a	3.44±0.09 ab	2.75±0.04 a	2.81±0.15 ab	1.36±0.11 a	1.22±0.05 a	0.11±0.01 a	9.36±0.59 a
M3	5.08±0.18 a	3.09±0.36 a	3.29±0.04 c	2.65±0.13 a	2.69±0.18 b	1.26±0.06 ab	1.05±0.12 b	0.10±0.01 a	9.46±0.55 a

处理	株高	茎粗	叶长	叶宽	根长	根粗	单株鲜重	单株干重	折干率
Treatment	Plant height/cm	Stem diameter/mm	Leaf length/cm	Leaf width/cm	Root length/cm	Root diameter/mm	Fresh weight per plant/g	Dry weight per plant/g	Drying rate/%
CK	4.99±0.28 a	2.99±0.20 a	3.38±0.02 bc	2.73±0.05 a	3.40±0.35 a	1.18±0.07 b	1.17±0.14 a	0.11±0.01 a	9.59±1.50 a
M1	5.05±0.28 a	3.01±0.22 a	3.48±0.04 ab	2.81±0.07 a	3.40±0.19 a	1.37±0.02 a	1.18±0.03 a	0.11±0.01 a	9.52±0.23 a
M2	5.07±0.10 a	3.32±0.19 a	3.44±0.09 ab	2.75±0.04 a	2.81±0.15 ab	1.36±0.11 a	1.22±0.05 a	0.11±0.01 a	9.36±0.59 a
M3	5.08±0.18 a	3.09±0.36 a	3.29±0.04 c	2.65±0.13 a	2.69±0.18 b	1.26±0.06 ab	1.05±0.12 b	0.10±0.01 a	9.46±0.55 a

处理	Δ株高	Δ茎粗	Δ叶长	Δ叶宽	Δ根长	Δ根粗	Δ单株鲜重	Δ单株干重	Δ折干率
Treatment	ΔPlant height/cm	ΔStem diameter/mm	ΔLeaf length/cm	ΔLeaf width/cm	ΔRoot length/cm	ΔRoot diameter/mm	ΔFresh weight per plant/g	ΔDry weight per plant/g	ΔDrying rate/%
CK	0.66± 0.28 a	-0.08± 0.20a	0.25± 0.02 ab	0.27± 0.05 a	1.00± 0.35 a	-0.02± 0.07b	0.26± 0.14 a	0.03± 0.01 a	0.86± 1.50 a
M1	0.72± 0.28 a	-0.06± 0.22a	0.35± 0.04 a	0.35± 0.07 a	1.00± 0.19 a	0.17± 0.02 a	0.27± 0.03 a	0.03± 0.01 a	0.79± 0.24 a
M2	0.74± 0.10 a	0.25± 0.19 a	0.31± 0.09 a	0.29± 0.04 a	0.40± 0.15 b	0.16± 0.11 a	0.31± 0.05 a	0.04± 0.01 a	0.63± 0.59 a
M3	0.75± 0.18 a	0.02± 0.36 a	0.16± 0.04 b	0.19± 0.13 a	0.29± 0.18 b	0.06± 0.06ab	0.14± 0.12 a	0.02± 0.01 a	0.73± 0.55 a

处理	Δ株高	Δ茎粗	Δ叶长	Δ叶宽	Δ根长	Δ根粗	Δ单株鲜重	Δ单株干重	Δ折干率
Treatment	ΔPlant height/cm	ΔStem diameter/mm	ΔLeaf length/cm	ΔLeaf width/cm	ΔRoot length/cm	ΔRoot diameter/mm	ΔFresh weight per plant/g	ΔDry weight per plant/g	ΔDrying rate/%
CK	0.66± 0.28 a	-0.08± 0.20a	0.25± 0.02 ab	0.27± 0.05 a	1.00± 0.35 a	-0.02± 0.07b	0.26± 0.14 a	0.03± 0.01 a	0.86± 1.50 a
M1	0.72± 0.28 a	-0.06± 0.22a	0.35± 0.04 a	0.35± 0.07 a	1.00± 0.19 a	0.17± 0.02 a	0.27± 0.03 a	0.03± 0.01 a	0.79± 0.24 a
M2	0.74± 0.10 a	0.25± 0.19 a	0.31± 0.09 a	0.29± 0.04 a	0.40± 0.15 b	0.16± 0.11 a	0.31± 0.05 a	0.04± 0.01 a	0.63± 0.59 a
M3	0.75± 0.18 a	0.02± 0.36 a	0.16± 0.04 b	0.19± 0.13 a	0.29± 0.18 b	0.06± 0.06ab	0.14± 0.12 a	0.02± 0.01 a	0.73± 0.55 a

处理	Δ总黄酮含量	Δ多糖含量	Δ总生物碱含量	Δ总氨基酸含量
Treatment	ΔTotal content of flavonoid/%	ΔThe content of polysaccharide/%	ΔThe content of alkaloid/%	ΔTotal content of amino acid/%
CK	-0.28±0.01 b	-0.61±0.27 b	-0.29±0.08 a	4.01±0.44 b
M1	0.05±0.12 a	-0.40±0.08 b	-0.25±0.03 a	5.83±0.27 a
M2	-0.35±0.16 b	1.63±0.17 a	-0.17±0.22 a	3.32±0.14 c
M3	0.12±0.14 a	2.42±0.61 a	-0.25±0.11 a	1.96±0.02 d

外源褪黑素对金线兰有效成分含量及抗氧化酶活性的影响

Effects of exogenous melatonin on active components content and antioxidase activity of Anoectochilus roxburghii

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处理	Δ叶绿素a	Δ叶绿素b	Δ叶绿素a+b	Δ类胡萝卜素a
Treatment	ΔChlorophyll a	ΔChlorophyll b	ΔChlorophyll a+b	ΔCarotenoid
CK	0.14±0.09 b	-0.08±0.03 b	0.06±0.11 b	0.39±0.07 b
M1	0.37±0.04 a	0.01±0.04 a	0.38±0.08 a	0.54±0.05 a
M2	0.45±0.08 a	0.04±0.01 a	0.49±0.08 a	0.59±0.03 a
M3	0.37±0.12 a	0.00±0.05 a	0.38±0.17 a	0.53±0.08 ab

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