›› 2012, Vol. 24 ›› Issue (5): 0-841.

• 论文 •    

贵州茶树地方品种的EST-SSR遗传多样性分析

牛素贞1,2,3,刘玉倩2,杨锦标4,樊卫国2,*
  

  1. 1贵州大学 林学院,贵州 贵阳 550025;2贵州大学 农学院,贵州 贵阳 550025;3贵州大学 茶叶工程技术研究中心,贵州 贵阳 550025;4贵定县茶叶产业发展管理办,贵州 贵定 551300
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2012-09-24 发布日期:2012-09-24

Analysis of genetic diversity of tea landrace resources in Guizhou by EST-SSR markers

NIU Su-zhen;;LIU Yu-qian;YANG Jin-biao;FAN Wei-guo;*   

  1. 1College of Forestry, Guizhou University,Guiyang 550025, China; 2College of Agriculture,Guizhou University,Guiyang 550025, China; 3Tea Engineer and Technology Research Center of Guizhou University,Guiyang 550025, China; 4Management Office of Tea Industry Development, Guiding 551300, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2012-09-24 Published:2012-09-24

摘要: 利用29个EST-SSR标记对贵州有代表性的25份地方茶树群体种和3份育成品种遗传多样性、亲缘关系和遗传分化进行了分析。结果表明,29对引物共检测到等位位点104个,平均等位基因3.58个,平均有效等位基因为2.85个,平均观测杂合度为0.56,平均期望杂合度为0.66,平均多态性信息含量为0.58,黔南地区茶树资源遗传多样性的上述5个参数均大于黔北地区。F\|统计量分析表明两个地区种群内近交系数、种群间近交系数、种群遗传分化和基因流分别为0.04,0.12,0.08,3.01。群体遗传结构显示,28份资源间的相似系数范围为0.39~0.89,可划分为4个类群,分别由4,5,8,11份资源组成。表明贵州地方茶树品种资源具有丰富的遗传多样性,资源间的遗传差异较大;聚类结果较客观地反映了群体结构和地域性差异等信息;黔南地区茶树资源遗传多样性程度高于黔北地区,两地区遗传分化较高,基因交流频率较低。

关键词: 茶树, 地方品种, EST-SSR, 遗传多样性

Abstract: Twenty-five tea landrace from Guizhou province, as well as three developed cultivar tea, were analyzed by 29 EST-SSR markers. A total of 104 alleles were identified with an average of 3.58 alleles per marker. The average of effective number of alleles, observed heterozygosity, expected heterozygosity and polymorphism information content were 2.85, 0.56, 0.66 and 0.58, respectively. The Qianbei tea germplasms showed lower values of the five indexes mentioned above, compared to the Qiannan Region. The Fis, Fit, Fst and gene flow were 0.04, 0.12, 0.08, and 3.01, respectively. The population structure analysis showed the genetic similarity coefficient ranged from 0.39 to 0.89,could divide all the 28 accessions into 4 groups. Tea germplasms were grouped into groupⅠ,Ⅱ,Ⅲ,Ⅳ, including 4,5,8,11 tea germplasms, respectively. It was suggested that the materials used in the experiment possessed a broad genetic differences and a higher genetic diversity. The results objectively reflect information including population structure and regional differences. The genetic diversity of tea germplasms in the Qianbei Region was lower than that of Qiannan Region. The tea germplasms in Qianbei Region showed richer genetic diversity than that in Qiannan Region, with relatively high genetic divergence and weak gene flow.

Key words: tea plant, landrace, EST-SSR, genetic diversity