浙江农业学报

• 园艺科学 • 上一篇    下一篇

外源水杨酸对盐胁迫栀子幼苗的缓解效应

  

  1. (1 阜阳师范学院 生物与食品工程学院,安徽 阜阳 236037;2 林木遗传育种国家重点实验室/中国林业科学研究院林业研究所,北京 100091; 3 安徽建筑大学 建筑与规划学院,安徽 合肥 230022)
  • 出版日期:2015-11-25 发布日期:2015-12-05

Ameliorating effects of exogenous salicylic acid on Gardenia jasminoides seedlings under salt stress

  1. (1 School of Biotechnology and Food Engineering,Fuyang Normal College, Fuyang 236037, China; 2 State Key Laboratory of Tree Genetics and Breeding,Research Institute of Forestry, Chinese Academy of Forestry Sciences, Beijing 100091, China; 3 College of Architecture & Urban Planning, Anhui Jianzhu University, Hefei 230022, China)
  • Online:2015-11-25 Published:2015-12-05

摘要: 以一年生栀子苗为试验材料,采用水培法,在150 mmol·L-1 NaCl胁迫条件下,通过研究不同浓度的外源水杨酸(SA),对栀子幼苗渗透调节物质含量及抗氧化系统等生理生化指标的影响,探讨SA对盐胁迫的缓解作用。结果表明:NaCl胁迫下,施加一定浓度的SA可使栀子幼苗叶片可溶性糖、可溶性蛋白、叶绿素、脯氨酸含量和抗氧化系统SOD,POD,CAT活性显著升高,叶片中相对电导率、MDA含量则显著降低,且0.5~1 mmol·L-1 SA处理效果最佳。研究显示:0.5~1 mmol·L-1 SA能够有效缓解盐胁迫对栀子幼苗造成的生理伤害,提高其抗盐性,而2~4 mmol·L-1SA处理则有可能加剧盐胁迫造成的伤害。

关键词: 栀子, 盐胁迫, 水杨酸, 抗氧化系统

Abstract: This study investigated the effect of exogenous salicylic acid (SA) on physiological characteristics of Gardenia jasminoides seedlings under salt stress. G. jasminoides seedlings were cultured with 150 mmol·L-1 NaCl and different concentrations(0, 05, 1, 2 and 4 mmol·L-1)of SA, physiological and biochemical indexes were studied, including osmolytes content and antioxidant system. The results showed that 150 mmol·L-1 NaCl significantly inhibited the physiological and biochemical indexes of G.jasminoides seedlings. Appropriate concentration of SA could improve chlorophyll, soluble sugar, soluble protein, free proline(Pro) contents, and superoxide(SOD), peroxidase(POD) and catalase(CAT) activities of G.jasminoides seedlings under 150 mmol·L-1 NaCl, meanwhile reduced their relative electric conductivity and malondialdehyde(MDA) content, especially the SA concentration of 0.5-1 mmol·L-1 showed the best effect. In conclusion, it was shown that SA with concentration of 0.5-1 mmol·L-1 could markedly reduce the physiological damages to G.jasminoides seedlings exposed to salt stress, and increase its salt resistance, but 2-4 mmol·L-1 SA were likely to exacerbate the salt stress.

Key words: Gardenia jasminoides, salt stress, salicylic acid, antioxidant system