浙江农业学报

• 作物科学 •    下一篇

海藻糖浸种对盐胁迫下扬麦19生理特性的影响

  

  1. (浙江农林大学 林业与生物技术学院,浙江 杭州 311300)
  • 出版日期:2016-08-25 发布日期:2016-08-04

Effects of soaking seeds in trehalose on physiological characteristics of wheat Yangmai19 under salt stress

  1. (College of Forestry & Biotechnology, Zhejiang A & F University, Hangzhou 311300, China)
  • Online:2016-08-25 Published:2016-08-04

摘要: 以扬麦19为材料,研究4个海藻糖溶液浓度(0、2、20、40 mmol·L-1)浸种对其种子萌发的影响。选择对种子萌发没有明显抑制效应的最适海藻糖溶液浓度(2 mmol·L-1),研究其对盐胁迫下(3 g·L-1 NaCl)扬麦19幼苗生长及生理特性的影响。结果表明,2 mmol·L-1海藻糖溶液浸种处理对扬麦19种子发芽率和幼苗株高无显著影响,但对幼苗单株干质量有显著促进作用。在盐胁迫下,2 mmol·L-1海藻糖浸种处理可显著提高扬麦19幼苗相对生长率、单株生物量、叶绿素相对含量和N含量,比水浸种处理分别提高200%、650%、1507%和925%;幼苗脯氨酸积累速度明显加快,K+含量及K+/Na+显著增加,Na+含量显著下降(P<005)。双因素方差分析表明,影响幼苗K+含量的程度依次为海藻糖>NaCl>海藻糖×NaCl。海藻糖浸种处理可以使扬麦19更快更早地积累脯氨酸,增强对K+的吸收,以适应盐胁迫环境。

关键词: 扬麦19, 海藻糖, 盐胁迫, 浸种, 生理特性

Abstract: To investigate the effect of trehalose pretreatment with different concentrations of 0, 2, 20 and 40 mmol·L-1 on seed germination, Yangmai19, a highyielding winter wheat cultivar, was selected as the experimental material. Furthermore, the optimal concentration of trehalose (2 mmol·L-1) was screened out to study its effect on growth and physiological characteristics of Yangmai19 under salt stress (3 g·L-1 NaCl). The results showed that the seed germination and plant height were not significantly affected in the pretreatment with 2 mmol·L-1 trehalose, but the dry weight per plant was significantly increased. Under salt stress, compared with the nontreated seeds, the relative growth rate, biomass per plant, relative chlorophyll content and N content in the pretreatment of 2 mmol·L-1 trehalose were significantly improved by 200%, 650%, 1507% and 925%, respectively. Proline accumulation rate was found to be significantly increased in the seedlings pretreated with 2 mmol·L-1 trehalose than the nontreated seeds, as well as the total K+ content and K+/Na+ ratio, while Na+ content was significantly decreased (P<005). Twofactor variance analysis showed that the factor orders of impacting K+ content was trehalose>NaCl>trehalose×NaCl. Trehalose pretreatment could improve the seedlings adaption to salt stress earlier, by increasing their proline accumulation and also increasing their K+ absorption.

Key words: wheat Yangmai19, trehalose, salt stress, soaking seed, physiological characteristics