响应面法优化螺旋藻培养基

doi:10.3969/j.issn.1004-1524.2017.02.18

浙江农业学报 ›› 2017, Vol. 29 ›› Issue (2): 307-314.DOI: 10.3969/j.issn.1004-1524.2017.02.18

响应面法优化螺旋藻培养基

谢丽丹, 王素英^*

天津市食品生物技术重点实验室,天津商业大学生物技术与食品科学学院,天津 300400

收稿日期:2016-09-09 出版日期:2017-02-15 发布日期:2017-03-06
通讯作者: 王素英,E-mail:wsying@tjcu.edu.cn
作者简介:谢丽丹(1992—),女,山西运城人,硕士,从事微生物资源前期开发研究。E-mail:13820159533@163.com
基金资助:
国家自然科学基金项目(31270050); 天津市高等学校创新团队(TD12-5049)

Optimization of Spirulina media by response surface methodology

XIE Lidan, WANG Suying^*

Tianjin Key Laboratory of Food Biotechnology, College of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin 300400, China

Received:2016-09-09 Online:2017-02-15 Published:2017-03-06

摘要/Abstract

摘要： 为了优化螺旋藻培养基的基本配方,以纯碱生产废料、硝酸钠(NaNO₃)、氯化钾(KCl)、磷酸二氢钾(KH₂PO₄)为自变量,以螺旋藻藻蓝蛋白含量为响应值,应用Box-Behnken中心组合原理建立数学模型,进行响应面分析(RSM),确定螺旋藻培养基的最佳配方。结果表明,螺旋藻培养基最佳配方为纯碱生产废料17 g、硝酸钠(NaNO₃)2 g、氯化钾(KCl)1 g、磷酸二氢钾(KH₂PO₄)1.6 g,在此条件下,螺旋藻藻蓝蛋白实际含量达到0.269 3 mg·L^-1,与室外养殖采用的半合成培养基相比,藻蓝蛋白含量提高了23%。

关键词: 螺旋藻, 响应面, 培养基优化, 藻蓝蛋白

Abstract: To optimize the basic formula of Spirulina medium,the effects of soda ash production wastes, sodium nitrate(NaNO₃), potassium chloride (KCl), potassium dihydrogen phosphate (KH₂PO₄) on the phycocyanin content of Spirulina were determined using the response surface methodology (RSM) . Results showed that the optimal formula of Spirulina medium was soda ash production wastes 17 g, NaNO₃ 2 g, KCl 1 g, KH₂PO₄ 1.6 g, which can achieve 0.269 3 mg·L^-1 phycocyanin, 23% higher than that of the semi-synthetic medium used in outdoor cultivation.

Key words: Spirulina, response surface, medium optimization, phycocyanin

中图分类号:

Q179.1

谢丽丹, 王素英. 响应面法优化螺旋藻培养基[J]. 浙江农业学报, 2017, 29(2): 307-314.

XIE Lidan, WANG Suying. Optimization of Spirulina media by response surface methodology[J]. , 2017, 29(2): 307-314.

参考文献

[1] 张文, 吴清平, 吴军林, 等. 螺旋藻营养保健价值及开发应用进展[J]. 食品与发酵科技, 2013 (3): 89-92.
ZHANG W, WU Q P, WU J L, et al. The nutrition health value and research progress of Spirulina[J]. Food and Fermentation Technology, 2013 (3): 89-92. (in Chinese with English abstract)
[2] 徐建红, 辛晓芸, 王爱业, 等. 螺旋藻的研究现状及进展[J]. 山西师范大学学报(自然科学版), 2003, 17(3): 57-63.
XU J H, XIN X Y, WANG A Y, et al. The advance of the studies on the application of Spindina[J]. Journal of Shanxi Normal University (Natural Science Edition), 2003, 17(3): 57-63.(in Chinese)
[3] 王政. 螺旋藻的营养价值及应用[J]. 才智, 2014 (27): 373.
WANG Z. Nutritional value and application of Spirulina[J]. Caizhi, 2014 (27): 373.(in Chinese)
[4] 刘剑娜, 杨海龙, 李怡萱, 等. 浅谈碱性食物螺旋藻的营养价值[J]. 食品安全导刊, 2013 (7): 56-57.
LIU J N, YANG H L, LI Y X, et al. Talking about the nutritional value of basic foods of Spirulina[J]. China Food Safety Magazine, 2013 (7): 56-57.(in Chinese)
[5] VO T S, NGO D H, KIM S K. Chapter 19-Nutritional and pharmaceutical properties of microalgal Spirulina[M]//KIM S K. Handbook of marine microalgae. London: Academic Press, 2015: 299-308.
[6] 李永强, 陈清香, 师文庆, 等. 光照对螺旋藻生长速率的影响[J]. 激光生物学报, 2014(1): 51-54.
LI Y Q, CHEN Q X, SHI W Q, et al. The effects of light on the growth rate of Spirulina[J]. Acta Laser Biology Sinica, 2014(1): 51-54. (in Chinese with English abstract)
[7] 潘曰磊, 邹宁, 梁妍, 等. 螺旋藻培养研究进展[J]. 生命科学仪器, 2008, 6(9): 48-50.
PAN Y L, ZOU N, LIANG Y, et al. The advance of research on Spriulina cultivate[J]. Life Science Instruments, 2008, 6(9): 48-50. (in Chinese with English abstract)
[8] 马增岭.阳光辐射变化对经济蓝藻螺旋藻形态、光合作用及生长的影响[D].汕头:汕头大学,2008.
MA Z L. Impacts of solar radiation on morphology, photosynthesis and growth of the economic cyanobacterium Arthospira(Spirulina) platensis[J].Shantou: Shantou University,2008.(in Chinese with English abstract)
[9] IVANOVA K G, STANKOVA K G, NIKOLOV V N, et al. The biliprotein C-phycocyanin modulates the early radiation response: A pilot study[J]. Mutation Research/Genetic Toxicology and Environmental Mutagenesis, 2010, 695(1): 40-45.
[10] 陈必链, 庄惠如, 王明兹, 等. 培养方法对钝顶螺旋藻生长的影响[J]. 福建师范大学学报(自然科学版), 2001, 17(1): 72-74.
CHEN B L, ZHUANG H R, WANG M Z, et al. Effect of cultivation methods on growth of Spirulina platensis[J]. Journal of Fujian Teachers University (Natural Science), 2001, 17(1): 72-74. (in Chinese)
[11] JEON H J, CHOI Y K, EOM H S, et al. Comparison among dry cell weight, chlorophyll a concentration, and amperometric signal during a batch cultivation of Spirulina maxima[J]. Sensors & Actuators B Chemical, 2014, 205: 9-11.
[12] FERREIRA S L C, BRUNS R E, FERREIRA H S, et al. Box-Behnken design: An alternative for the optimization of analytical methods[J]. Analytica Chimica Acta, 2007, 597(2): 179-186.
[13] 冯强, 滕建文, 黄丽, 等. 响应面法优化螺旋藻自溶条件的研究[J]. 食品科技, 2011(7): 95-98.
FENG Q, TENG J W, HUANG L, et al. Optimizing condition for autolysis of Spirulina using response surface methodology(RSM)[J]. Food Science and Technology, 2011(7): 95-98. (in Chinese with English abstract)
[14] 谯顺彬,迟海洋,张奕婷,等.螺旋藻混合营养培养基响应面法的优化研究[J].食品科学,2009,30(7):109-113.
QIAO S B, CHI H Y, ZHANG Y T, et al. Optimization of mixotrophic medium for Spirulina culture by response surface methodogy[J]. Food Science, 2009, 30(7):109-113.(in Chinese with English abstract)
[15] 宋倩, 赵声兰, 刘彬球, 等. 响应面法优化核桃壳总黄酮提取工艺的研究[J]. 食品工业科技, 2013, 34(11): 214-217.
SONG Q, ZHAO S L, LIU B Q, et al. Optimization of extraction of total flavonoids from walnut shell using response surface methodology[J]. Science and Technology of Food Industry, 2013,34(11): 214-217. (in Chinese with English abstract)
[16] 李莉, 顾赛麒, 王锡昌, 等. 响应面法优化酶法提取大鲵皮胶原蛋白工艺[J]. 中国水产科学, 2013, 20(4): 876-883.
LI L, GU S L, WANG X C, et al. Optimization of conditions for the extraction of pepsin-soluble collagen from the skin of Chinese giant salamander[J]. Journal of Fishery Sciences of China, 2013,20 (4): 876-883. (in Chinese)
[17] ZHENG J, SHAO G, SHEN X. Synergistic interactions of chemical additives on the strength development of silicate cement by a box-behnken model optimization[J]. Journal of the Japanese Forestry Society, 2014, 131(22): 285-293.
[18] YE Z, WANG W, YUAN Q, et al. Box-Behnken design for extraction optimization, characterization and in vitro antioxidant activity of Cicer arietinum L. hull polysaccharides[J]. Carbohydrate Polymers, 2016, 147(20): 354-364.
[19] 张嘉, 沈丹虹, 郑晓冬, 等. 1株高产油脂酵母菌株的诱变选育及其发酵条件研究[J]. 浙江大学学报(农业与生命科学版), 2009, 35 (5): 497-502.
ZHANG J, SHEN D H, ZHENG X D, et al. Researches on mutation breeding and fermentation condition of oleaglnous yeast[J]. Journal of Zhejiang University (Agriculture & Life Sciences), 2009, 35(5): 497-502.(in Chinese with English abstract)
[20] 刘国荣, 张郡莹, 王成涛, 等. 响应面法优化双歧杆菌B04代谢产细菌素的发酵条件[J]. 食品科学, 2013, 34(3): 147-152.
LIU G R, ZHANG J Y, WANG C T, et al. Optimization of fermentation conditions for bacteriocin production from bifidobacterium animal b04 by response surface methodology[J]. Food Science, 2013, 34(3): 147-152.(in Chinese with English abstract)
[21] 田华, 于斐, 张义明. 螺旋藻混合营养培养基配方的优化研究[J]. 食品科技, 2008,33(12): 32-34.
TIAN H, YU F, ZHANG Y M. Optimization of mixotrophism culture medium of S.platensis[J]. Food Science and Technology, 2008,33(12): 32-34.(in Chinese with English abstract)

响应面法优化螺旋藻培养基

Optimization of Spirulina media by response surface methodology

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