玉米细胞核雄性不育突变体K305ms的生理生化分析

doi:10.3969/j.issn.1004-1524.2018.08.02

浙江农业学报 ›› 2018, Vol. 30 ›› Issue (8): 1281-1287.DOI: 10.3969/j.issn.1004-1524.2018.08.02

玉米细胞核雄性不育突变体K305ms的生理生化分析

汪燕^1,2, 石海春^1,3, 余学杰^1,3, 赵长云³, 柯永培^1,3,*

1.四川农业大学农学院,四川成都 611130;
2.贵州师范大学植物遗传育种研究所荞麦产业技术研究中心,贵州贵阳 550001;
3.四川农大正红生物技术有限责任公司,四川成都 610213

收稿日期:2017-10-11 出版日期:2018-08-25 发布日期:2018-08-28
通讯作者: 柯永培,E-mail: keyp169@163.com
作者简介:汪燕(1985—),女,重庆人,博士,助理研究员,从事玉米雄性不育及育性恢复机制等相关研究。E-mail: yanwanguf@163.com
基金资助:
四川科技厅省重点支撑项目(Grand 2011FZ0119,YZGG2011-3-11)

Analysis of physiological and biochemical characteristics of maize male sterile mutant K305ms

WANG Yan^1,2, SHI Haichun^1,3, YU Xuejie^1,3, ZHAO Changyun³, KE Yongpei^1,3,*

1. College of Agronomy, Sichuan Agricultural University, Chengdu 611130, China;
2. Research Center of Buckwheat Industry Technology, Institute of Plant Genetics and Breeding, Guizhou Normal University, Guiyang 550001, China;
3. Sichuan Nongda Zhenghong Bio. Co., Ltd, Chengdu 610213, China

Received:2017-10-11 Online:2018-08-25 Published:2018-08-28

摘要/Abstract

摘要： 为探讨⁶⁰Co-γ辐射诱变获得的玉米细胞核雄性不育突变体K305ms的生理生化特征,以不育株K305ms及其姊妹交群体中的可育株K305F为材料,对雄花发育不同时期花药和颖壳中的可溶性蛋白含量、游离脯氨酸含量及超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性进行了测定。结果表明,与可育株K305F相比,不育株K305ms雄花发育过程中花药与颖壳的可溶性蛋白含量和游离脯氨酸含量均不同程度降低,且K305ms花药的SOD和CAT活性明显降低,而POD活性显著或极显著升高。说明玉米细胞核雄性不育可能与花药发育过程中物质代谢和抗氧化系统异常有关。

关键词: 玉米, 细胞核雄性不育, 突变体K305ms, 生理生化特性

Abstract: In order to understand physiological and biochemical characteristics of the maize genic male sterile mutant K305ms induced by⁶⁰Co-γ irradiation, male sterile mutant K305ms and fertile plant K305F from their sister-crossed line were used as materials. The content change of soluble protein, free proline, and the activity of superoxide dismutase (SOD), catalase (CAT), peroxidase (POD) during the anther development stage were detected. The main results were showed as follows: The soluble protein content and free proline content in anther and glume of K305ms mutant were lower than fertile plant K305F during anther development stage; Moreover, the activities of SOD and CAT in anther of K305ms mutant were significant lower than those in K305F, whereas the POD activity was significant higher. The results indicated that genic male sterility of maize was related to abnormal metabolism of soluble protein and the disordered reactive oxygen scavenging enzyme system (ROS) during the development of anther.

Key words: maize, genic male sterility, K305ms mutant, physiological and biochemical characteristics

中图分类号:

汪燕, 石海春, 余学杰, 赵长云, 柯永培. 玉米细胞核雄性不育突变体K305ms的生理生化分析[J]. 浙江农业学报, 2018, 30(8): 1281-1287.

WANG Yan, SHI Haichun, YU Xuejie, ZHAO Changyun, KE Yongpei. Analysis of physiological and biochemical characteristics of maize male sterile mutant K305ms[J]. , 2018, 30(8): 1281-1287.

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玉米细胞核雄性不育突变体K305ms的生理生化分析

Analysis of physiological and biochemical characteristics of maize male sterile mutant K305ms

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