京海黄鸡柔嫩艾美尔球虫感染对脾脏和盲肠IL-6、IL-8、CCLi2基因表达量的影响及其相关性

doi:10.3969/j.issn.1004-1524.2019.01.05

浙江农业学报 ›› 2019, Vol. 31 ›› Issue (1): 39-46.DOI: 10.3969/j.issn.1004-1524.2019.01.05

京海黄鸡柔嫩艾美尔球虫感染对脾脏和盲肠IL-6、IL-8、CCLi2基因表达量的影响及其相关性

辛世杰^1,2, 王晓慧^1,2, 戴国俊^1,2,*, 安婷婷^1,2, 张涛^1,2, 张跟喜^1,2, 谢恺舟^1,2, 王金玉^1,2, 王宏胜³

1.扬州大学动物科学与技术学院,江苏扬州 225009;
2.江苏省动物遗传育种与分子设计重点实验室,江苏扬州 225009;
3.江苏京海禽业集团有限公司,江苏海门 226100

收稿日期:2018-03-14 出版日期:2019-01-25 发布日期:2019-04-09
通讯作者: 戴国俊,E-mail: daigj@yzu.edu.cn
作者简介:辛世杰(1992—),男,山东日照人,硕士研究生,主要从事家禽生产及抗病育种研究。E-mail: 1028638864@qq.com
基金资助:
江苏省农业三新工程(SXGC [2017]298); 江苏现代农业产业技术体系建设专项资金(JATS[2018]303); “十二五”国家科技支撑计划(2014BAD13B02); 江苏省高校优势学科建设工程(PAPD); 国家现代农业产业技术体系专项资金(CARS-41-G23)

Effect and correlation analysis of Eimeria tenella infection on IL-6, IL-8 and CCLi2 genes expression in spleen and caecum of Jinghai Yellow Chicken (Gallus gallus)

XIN Shijie^1,2, WANG Xiaohui^1,2, DAI Guojun^1,2,*, AN Tingting^1,2, ZHANG Tao^1,2, ZHANG Genxi^1,2, XIE Kaizhou^1,2, WANG Jinyu^1,2, WANG Hongsheng³

1. College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China;
2. Key Lab for Animal Genetics, Breeding and Reproduction and Molecular Design of Jiangsu Province, Yangzhou 225009, China;
3. Jiangsu Jinghai Poultry Group Co. Ltd., Haimen 226100, China

Received:2018-03-14 Online:2019-01-25 Published:2019-04-09

摘要/Abstract

摘要： 白介素6(IL-6)、白介素8(IL-8)和CC趋化因子配体2(CCLi2)3种细胞因子在炎症反应以及机体免疫方面发挥重要作用。为了揭示球虫感染对脾脏和盲肠中IL-6、IL-8和CCLi2的表达量的影响,以京海黄鸡(Gallus gallus)为试验材料,设球虫感染组和非感染的对照组,利用实时荧光定量PCR(qRT-PCR)检测了脾脏与盲肠组织中IL-6、IL-8和CCLi2的mRNA的相对表达量,比较同一组织感染组与对照组间3个基因表达量的差异,同时分析同一处理组的同一组织内、不同组织间3个基因表达量的相关性。研究结果表明:感染组脾脏组织IL-6、IL-8和CCLi2三个基因的表达量均显著高于对照组(P<0.05),其中IL-6基因达极显著水平(P<0.01);感染组盲肠组织中IL-6、IL-8和CCLi2的表达量均极显著地高于对照组(P<0.01)。相关性分析结果表明,感染组脾脏组织中IL-6、IL-8和CCLi2的表达量之间均存在极显著的正相关(P<0.01);在感染组盲肠组织中IL-8、CCLi2与IL-6基因的表达量存在极显著的正相关(P<0.01),但IL-8与CCLi2基因表达量相关不显著(P>0.05);在对照组脾脏组织中,IL-6、IL-8与CCLi2间存在极显著的正相关(P<0.01),其他基因间相关均不显著(P>0.05);对照组盲肠组织中IL-6、IL-8和CCLi2之间均存在极显著的正相关(P<0.01);此外,无论感染组还是对照组,脾脏和盲肠组织间3个基因的表达量相关性均不显著(P>0.05)。以上结果表明,IL-6、IL-8和CCLi2可能在京海黄鸡球虫感染过程中发挥重要作用,研究结果对进一步探讨这3个基因在球虫病抗病育种中的作用提供了依据,对京海黄鸡球虫病抗病育种具有重要意义。

关键词: 京海黄鸡, 盲肠, 脾脏, IL-6, IL-8, CCLi2, 基因表达, 相关性分析

Abstract: Interleukin 6 (IL-6), interleukin 8 (IL-8) and CC motif chemokine ligand 2 (CCLi2) play an important role in the inflammatory response and immune. In this study, in order to reveal the expression relationships between the three genes in spleen, caecum and between spleen and caecum at coccidiosis infected and non-infected state, Jinghai Yellow Chicken(Gallus gallus) were divided into coccidiosis infection and non-infection group, the relative expression of mRNA of IL-6, IL-8 and CCLi2 in spleen and caecum was detected by quantitative real-time polymerase chain reaction (qRT-PCR), the relative expression difference of the three genes were compared between infection and control group in the same tissue, meanwhile, the relative expression correlation were analyzed among the three genes in the same and different tissues within the same group. The results showed that the relative expression levels of IL-6, IL-8 and CCLi2 genes in spleen tissue of infected group were all higher than those of the control group (P<0.05), and the relative expression level of IL-6 gene in infected group was extremely significant different from control group (P<0.01); the relative expression levels of IL-6, IL-8 and CCLi2 genes in the caecum tissue of infected group were all extremely significant different from that of the control group (P<0.01). The correlation analysis showed that the correlation coefficient between the expression of IL-6, IL-8 and CCLi2 genes in spleen tissues of infected group were all positively related (P<0.01); in caecum tissue of infected group, there were extremely significant positive correlations(P<0.01) among the relative expression of the IL-8, CCLi2 with IL-6 genes, but the relative expression correlation between IL-8 and CCLi2 genes was not significant correlated (P>0.05); in the spleen tissue of the control group, there were extremely significant positive correlations (P<0.01) between the relative expression of IL-6, IL-8 with CCLi2 genes, and the correlation between other genes in the control group was not significant (P>0.05); there were extremely significant positive correlations (P<0.01) among the three genes of IL-6, IL-8 and CCLi2 in the caecum tissue of the control group; the correlation analysis results also showed that the expression of the three genes was not significantly correlated (P>0.05) between spleen and caecum tissue, regardless of the infection group or the control group. All of the results indicated that IL-6, IL-8 and CCLi2 might play an important role in the process of Jinghai Yellow Chicken coccidiosis infection, and the results provided the basis for further exploring the role of these three genes in the genetic breeding of coccidiosis resistance, and it had an important significance for Jinghai Yellow Chicken genetic breeding of coccidiosis resistance.

Key words: Jinghai Yellow Chicken, caecum, spleen, IL-6, IL-8, CCLi2, gene expression, correlation analysis

中图分类号:

S831.2

辛世杰, 王晓慧, 戴国俊, 安婷婷, 张涛, 张跟喜, 谢恺舟, 王金玉, 王宏胜. 京海黄鸡柔嫩艾美尔球虫感染对脾脏和盲肠IL-6、IL-8、CCLi2基因表达量的影响及其相关性[J]. 浙江农业学报, 2019, 31(1): 39-46.

XIN Shijie, WANG Xiaohui, DAI Guojun, AN Tingting, ZHANG Tao, ZHANG Genxi, XIE Kaizhou, WANG Jinyu, WANG Hongsheng. Effect and correlation analysis of Eimeria tenella infection on IL-6, IL-8 and CCLi2 genes expression in spleen and caecum of Jinghai Yellow Chicken (Gallus gallus)[J]. , 2019, 31(1): 39-46.

参考文献

[1] BLAKE D P, TOMLEY F M.Securing poultry production from the ever-present eimeria challenge[J]. Trends in Parasitology, 2014, 30(1):12-19.
[2] SHARMAN P A, SMITH N C, WALLACH M G, et al.Chasing the golden egg: vaccination against poultry coccidiosis[J]. Parasite Immunology, 2010, 32(8):590-598.
[3] LI G Q, KANU S, XIAO S M, et al.Responses of chickens vaccinated with a live attenuated multi-valent ionophore-tolerant Eimeria vaccine[J]. Veterinary Parasitology, 2005, 129(3):179-186.
[4] 林雨鑫. 京海黄鸡柔嫩艾美耳球虫感染后盲肠转录组分析及其杂交配套系亲本的抗性评价[D]. 扬州:扬州大学,2015.
LIN Y X.RNA sequencing analysis of chicken cecum tissues following E. tenella infection and coccidiosis evaluation of Jinghai Yellow Chicken cross-breeding system parents[D]. Yangzhou: Yangzhou University, 2015.(in Chinese with English abstract)
[5] NISHIMICHI N, KAWASHIMA T, HOJYO S, et al.Characterization and expression analysis of a chicken interleukin-6 receptor alpha[J]. Developmental & Comparative Immunology, 2006, 30(4):419-429.
[6] NEURATH M F, FINOTTO S.IL-6 signaling in autoimmunity, chronic inflammation and inflammation-associated cancer[J]. Cytokine & Growth Factor Reviews, 2011, 22(2):83-89.
[7] COUSSENS L M, WERB Z.Inflammation and cancer[J]. Nature, 2002, 420(6917):860-867.
[8] 陈卫红. 鸭IL-8 cDNA克隆与基因组结构分析及其重组蛋白的表达[D]. 北京:中国农业大学, 2005.
CHEN W H. cDNA cloning, genomic structure and recombinant protein expression of the duck IL-8 [D].Beijing: China Agricultural University, 2005.(in Chinese with English abstract)
[9] 曹禹, 张效林, 刘丹,等. 趋化因子CCL2对血小板p38MAPK-HSP27通路的影响[J].解放军医学杂志, 2017, 42(5):407-412.
CAO Y, ZHANG X L, LIU D, et al.Effects of chemokine CCL2 on the p38MAPK-HSP27 pathway in the platelets[J]. Medical Journal of Chinese People's Liberation Army, 2017, 42(5):407-412.(in Chinese with English abstract)
[10] 方强. 家禽Toll样受体基因的克隆、序列分析及不同品种鸡抗沙门菌感染天然免疫应答研究[D].扬州:扬州大学,2012.
FANG Q.Cloning and sequence analysis of avian toll-like receptors genes and the differences of innate immunity in different chicken lines against salmonella infection[D].Yangzhou: Yangzhou University, 2012.(in Chinese with English abstract)
[11] 栾维民, 杨树宝, 杨丽华,等. 鸡脾脏中T淋巴细胞及其亚群的发育[J]. 中国兽医学报, 2008, 28(6):684-687.
LUAN W M, YANG S B, YANG L H, et al.Development of Tlymphocyte subpopulations in chicken spleen[J]. Chinese Journal of Veterinary Science, 2008, 28(6):684-687.(in Chinese with English abstract)
[12] 刘伟杰, 孔正杰, 李海燕,等.鸭源NDV激活鸡和鸭先天性免疫应答的比较研究[J]. 农业生物技术学报, 2017, 25(2):307-315.
LIU W J, KONG Z J, LI H Y, et al.Comparison of host innate immune response of chicken (Gallus gallus) and duck (Anas platyrhynchos) infected with duck-origin Newcastle disease virus[J]. Journal of Agricultural Biotechnology, 2017, 25(2):307-315.(in Chinese with English abstract)
[13] FERNANDO F S, OKINO C H, SILVA K R, et al.Increased expression of Interleukin-6 related to nephritis in chickens challenged with an Avian infectious bronchitis virus variant[J]. Clinical Science, 2015, 35(3):216-222.
[14] 赵娜, 李烨初, 张小荣,等. 鸡白细胞介素6研究进展[J]. 上海畜牧兽医通讯, 2013(1):16-17.
ZHAO N, LI Y C, ZHANG X R, et al.Research progress of chicken interleukin-6[J]. Shanghai Journal of Animal Husbandry and Veterinary Medicine, 2013(1):16-17. (in Chinese)
[15] BANNERMAN D D, PAAPE M J, LEE J W, et al.Escherichia coli and Staphylococcus aureus elicit differential innate immune responses following intramammary infection[J]. Clinical and Diagnostic Laboratory Immunology, 2004, 11(3):463-472.
[16] 张鑫宇, 秦爱建, 金文杰,等. 马立克氏病病毒类白细胞介素8(vIL-8)基因的克隆和表达[J]. 中国兽医学报, 2003, 23(6):521-524.
ZHANG X Y, QIN A J, JIN W J, et al.Expression of viral interleukin-8 gene of marek's disease virus in Escherichia coli[J]. Chinese Journal of Veterinary Science, 2003, 23(6):521-524. (in Chinese with English abstract)
[17] MIN W, LILEHOJ H S, BURNSIDE J, et al.Adjuvant effects of IL-1β, IL-2, IL-8, IL-15, IFN-α, IFN-γ TGF-β4, and lymphotactin, on DNA vaccination against Eimeria acervulina[J]. Vaccine, 2001, 20(1):267-274.
[18] O'CONNOR T, BORSIG L, HEIKENWALDER M. CCL2-CCR2 signaling in disease pathogenesis[J]. Endocrine Metabolic & Immune Disorders Drug Targets, 2015, 15(2):105-118.
[19] QIAN B Z, LI J, ZHANG H, et al.CCL2 recruits inflammatory monocytes to facilitate breast tumor metastasis[J]. Nature, 2011, 475(7355):222-225.
[20] MARTIN A P, RANKIN S, PITCHFORD S, et al.Increased expression of CCL2 in insulin-producing cells of transgenic mice promotes mobilization of myeloid cells from the bone marrow, marked insulitis, and diabetes.[J]. Diabetes, 2008, 57(11):3025-3033.
[21] MA Y, MATTAROLLO S R, ADJEMIAN S, et al.CCL2/CCR2-dependent recruitment of functional antigen-presenting cells into tumors upon chemotherapy[J]. Cancer Research, 2014, 74(2):436-445.
[22] BARON V T, PIO R, JIA Z, et al.Early growth response 3 regulates genes of inflammation and directly activates IL6 and IL8 expression in prostate cancer[J]. British Journal of Cancer, 2015, 112(4):755-764.
[23] KOGUT M H, ROTHWELL L, KAISER P.Differential regulation of cytokine gene expression by avian heterophils during receptor-mediated phagocytosis of opsonized and nonopsonized Salmonella enteritidis[J]. Journal of Interferon & Cytokine Research, 2003, 23(6):319-327.
[24] LUTHER S A, CYSTER J G.Chemokines as regulators of T cell differentiation[J]. Nature Immunology, 2001, 2(2):102-107.
[25] SWAGGERTY C L, PEVZNER I Y, KOGUT M H.Selection for pro-inflammatory mediators produces chickens more resistant to Eimeria tenella[J]. Poultry Science, 2015, 94(1):37-42.
[26] SWAGGERTY C L, KOGUT M H, FERRO P J, et al.Differential cytokine mRNA expression in heterophils isolated from Salmonella-resistant and -susceptible chickens[J]. Insect Science, 2004, 113(1):139-148.

京海黄鸡柔嫩艾美尔球虫感染对脾脏和盲肠IL-6、IL-8、CCLi2基因表达量的影响及其相关性

Effect and correlation analysis of Eimeria tenella infection on IL-6, IL-8 and CCLi2 genes expression in spleen and caecum of Jinghai Yellow Chicken (Gallus gallus)

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	黄咏明, 宋放, 王策, 姚京磊, 王志静, 何利刚, 吴黎明, 蒋迎春. 根系修剪对枳生长及相关基因表达的影响[J]. 浙江农业学报, 2021, 33(2): 270-277.
[2]	徐秀红, 刘金亮, 李栋成, 刘仁祥. 不同类型烟草种质的烟碱含量变化与相关基因表达水平[J]. 浙江农业学报, 2020, 32(9): 1555-1563.
[3]	刘坤举, 张小辉, 庞有志, 赵淑娟, 祁艳霞, 王乾昆. 朝鲜鹌鹑GNAS基因表达、克隆及其多态性与羽色的相关性[J]. 浙江农业学报, 2020, 32(8): 1369-1377.
[4]	李秋玲, 齐颖, 王琛, 张一名, 王新妤, 尚校兰, 贾永红, 李美茹, 储明星. 热应激对中国荷斯坦牛乳腺组织基因表达及信号通路的影响[J]. 浙江农业学报, 2020, 32(5): 770-778.
[5]	卢祎, 高有领, 王水涛, 何盛盛. MicroRNA-499对中华鳖脂类代谢相关基因表达的影响[J]. 浙江农业学报, 2020, 32(5): 798-803.
[6]	沈迪, 陈龙正, 路晓华, 陶建平, 冯顾城, 刘洁霞, 冯凯, 尹莲, 丁旭, 贾丽丽, 徐志胜, 刘惠吉, 熊爱生. 苏南地区29个秋冬茬芹菜品种资源评价[J]. 浙江农业学报, 2020, 32(4): 653-660.
[7]	李维芳, 王春蕾, 王妮, 邓雨正, 姚彦东, 魏丽娟, 廖伟彪. 一氧化氮影响植物不定根发生的研究进展[J]. 浙江农业学报, 2020, 32(4): 742-752.
[8]	白俊艳, 卢军浩, 付学言, 武晓红, 杨又兵, 雷莹, 庞有志, 卢小宁, 巩慧荣, 胡陆星, 刘红涛, 樊红灯, 曹恒, 时坤鹏, 陈梦柯, 马永康. 蛋用鹌鹑IGF-1R基因的多态性与体尺性状相关性分析[J]. 浙江农业学报, 2020, 32(3): 398-405.
[9]	秦玲, 张鑫, 荣春笑, 莫传园, 范露, 闫婕, 孟莹, 张满让. 苹果多胺氧化酶(PAO)基因家族鉴定与表达分析[J]. 浙江农业学报, 2020, 32(2): 262-273.
[10]	张政, 王晓荣, 钱虹, 张兰, 颜鹏, 张丽平, 张新富, 李鑫, 韩文炎. 炭疽病对茶树叶片光合特性的影响[J]. 浙江农业学报, 2020, 32(11): 2020-2026.
[11]	张爱菊, 刘士力, 刘金殿, 张根芳, 周志明. 一种三角帆蚌肌浆网Ca²⁺-ATP酶基因cDNA的全长克隆与表达分析[J]. 浙江农业学报, 2019, 31(4): 545-555.
[12]	陈洪强, 夏惠, 王进, 邓群仙, 梁东, 吕秀兰, 唐礼平. 葡萄STS基因家族的鉴定与表达分析[J]. 浙江农业学报, 2019, 31(3): 401-407.
[13]	李琴, 何敏, 赵飘, 陈小梅, 吕莹莹. 白介素21在42日龄中国黄羽鹌鹑盲肠扁桃体中的定位[J]. 浙江农业学报, 2018, 30(9): 1476-1481.
[14]	张卓, 辜彦霏, 阳莎莎, 王亚超, 邓俊良, 任志华. DON、ZEA联合染毒对体外培养鸡脾脏淋巴细胞分泌IL-2、IL-4、IL-10和IFN-γ的影响[J]. 浙江农业学报, 2018, 30(7): 1110-1115.
[15]	宁秋燕, 范凯, 王敏, 石元值. 茶树根系XTHs和伸展蛋白对不同浓度铝的响应[J]. 浙江农业学报, 2018, 30(6): 961-969.