玉米ZmGS5基因的克隆及其对转基因拟南芥种子发育的影响

doi:10.3969/j.issn.1004-1524.2019.04.01

浙江农业学报 ›› 2019, Vol. 31 ›› Issue (4): 513-518.DOI: 10.3969/j.issn.1004-1524.2019.04.01

玉米ZmGS5基因的克隆及其对转基因拟南芥种子发育的影响

何春梅, 王娟, 董瑞, 刘春晓, 刘强, 关海英, 汪黎明, 徐相波^*, 刘铁山

山东省农业科学院玉米研究所,小麦玉米国家工程实验室,农业农村部黄淮海北部玉米生物学与遗传育种重点实验室,山东济南 250100

收稿日期:2018-07-16 出版日期:2019-04-25 发布日期:2019-04-19
通讯作者: *徐相波,E-mail: xuxbo2008@126.com
作者简介:何春梅(1978-),女,山东莱芜人,博士,副研究员,主要从事玉米遗传育种研究。E-mail: chunmeihe11@163.com
基金资助:
中国博士后科学基金(2017M622244);山东省农业科学院青年科研基金(2014QNM30)

Cloning and characterization of maize ZmGS5 in regulating seed development of Arabidopsis thaliana

HE Chunmei, WANG Juan, DONG Rui, LIU Chunxiao, LIU Qiang, GUAN Haiying, WANG Liming, XU Xiangbo^*, LIU Tieshan

Maize Research Institute, Shandong Academy of Agricultural Sciences, National Engineering Laboratory of Wheat and Maize, Key Laboratory of Biology and Genetic Improvement of Maize in Northern Yellow-Huai River Plain, Ministry of Agriculture and Rural Affairs, Jinan 250100, China

Received:2018-07-16 Online:2019-04-25 Published:2019-04-19

摘要/Abstract

摘要： 植物种子发育与产量密切相关,研究发现玉米ZmGS5基因与其籽粒发育呈显著性正相关。本研究利用同源克隆技术克隆玉米ZmGS5基因,该基因编码区全长1 491 bp,编码含496个氨基酸的丝氨酸羧肽酶。通过构建ZmGS5过表达载体,采用花序浸染法遗传转化拟南芥,观察转基因拟南芥植株表型及种子变化,初步解析ZmGS5在调控种子发育过程中的功能。结果表明,转基因拟南芥植株莲座叶直径增加,叶片增大,生物量、每株角果数和种子千粒重及种子大小均显著增加。该研究结果初步揭示了玉米ZmGS5基因在调控作物生长发育和产量中发挥重要作用。

关键词: 玉米, ZmGS5, 遗传转化, 拟南芥, 种子大小

Abstract: Seed development is closely related with yield. Genome comparative analysis had revealed that maize ZmGS5 was significantly related with kernel development. In this study, the maize ZmGS5 was cloned, the open reading frame of ZmGS5 was 1 491 bp in length and encoded a 496 aa serine carboxypeptidase. The over-expression construct of ZmGS5 was then transformed into Arabidopsis by floral-dipping method. Through observation of the phenotypic alterations of the transgenic lines, functions of ZmGS5 in regulating growth and seed development was preliminarily revealed. These results showed that the transgenic lines over-expressing ZmGS5 could increase rosette diameter, enlarged leaf size and improved biomass, number of siliques per plant, one-thousand-seed weight and seed size. These results demonstrated that ZmGS5 played important roles in regulating plant growth especially in the seed size.

Key words: maize, ZmGS5, genetic transformation, Arabidopsis, seed size

中图分类号:

S513

何春梅, 王娟, 董瑞, 刘春晓, 刘强, 关海英, 汪黎明, 徐相波, 刘铁山. 玉米ZmGS5基因的克隆及其对转基因拟南芥种子发育的影响[J]. 浙江农业学报, 2019, 31(4): 513-518.

HE Chunmei, WANG Juan, DONG Rui, LIU Chunxiao, LIU Qiang, GUAN Haiying, WANG Liming, XU Xiangbo, LIU Tieshan. Cloning and characterization of maize ZmGS5 in regulating seed development of Arabidopsis thaliana[J]. , 2019, 31(4): 513-518.

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玉米ZmGS5基因的克隆及其对转基因拟南芥种子发育的影响

Cloning and characterization of maize ZmGS5 in regulating seed development of Arabidopsis thaliana

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