糜子矮秆突变体“819”矮秆基因的遗传学分析

doi:10.3969/j.issn.1004-1524.2020.01.03

浙江农业学报 ›› 2020, Vol. 32 ›› Issue (1): 20-27.DOI: 10.3969/j.issn.1004-1524.2020.01.03

糜子矮秆突变体“819”矮秆基因的遗传学分析

贾小平¹, 王振山¹, 朱学海^2,*, 杨德智², 寇淑君², 刘星星¹

1. 河南科技大学农学院,河南洛阳 471000;
2. 张家口市农业科学院黍子研究所,河北张家口 075000

收稿日期:2019-08-01 出版日期:2020-01-25 发布日期:2020-03-11
通讯作者: ^*朱学海,E-mail: 924180928@qq.com
作者简介:贾小平(1973—),男,内蒙古包头人,博士,副教授,主要从事谷子和糜子的分子生物学研究。E-mail: jiaxiaoping2007@163.com
基金资助:
河北省科技厅项目(17396310D)

Genetic analysis of dwarf gene for dwarf mutant“819” in Panicum miliaceum L.

JIA Xiaoping¹, WANG Zhenshan¹, ZHU Xuehai^2,*, YANG Dezhi², KOU Shujun², LIU Xingxing¹

1. College of Agronomy, Henan University of Science and Technology, Luoyang 471000, China;
2. Institute of Broomcorn Millet, Zhangjiakou Academy of Agricultural Sciences, Zhangjiakou 075000, China

Received:2019-08-01 Online:2020-01-25 Published:2020-03-11

摘要/Abstract

摘要： 为揭示糜子矮秆突变体“819”矮秆基因的遗传规律,为后续定位矮秆突变基因提供理论基础,研究调查了糜子矮秆突变体“819”与高秆材料J12,以及它们杂交产生的674个F₂代个体的株高、穗长、穗颈长、分蘖数、茎节数、二级枝梗长度1、二级枝梗长度2、二级枝梗长度3、二级枝梗间距1、二级枝梗间距2共10个性状,进一步利用方差分析、卡方检验、相关及回归分析、主成分分析对这些性状进行研究。结果表明,糜子矮秆突变体“819”的矮秆性状是由单基因控制的隐性性状;株高与穗长、穗颈长、分蘖数、茎节数、二级枝梗长度1、二级枝梗长度2、二级枝梗长度3、二级枝梗间距1呈极显著正相关(P<0.01),与二级枝梗间距2呈显著正相关(P<0.05);株高(Y)对其他农艺性状的回归方程为:Y=-18.446+1.491X₁+1.222X₂+6.827X₄+1.319X₇+0.746X₈,回归方程的拟合度为0.811,回归方程经显著性检验达到极显著水平(P<0.01),可以利用回归方程对株高进行预测;糜子F₂群体的10个性状进行主成分分析获得3个主成分,这3个主成分的累积贡献率高达72.656%,3个主成分分别被命名为长度因子、茎节数因子、枝梗间距因子。研究结果为矮秆基因定位、矮秆后代的评价选择提供了依据。

关键词: 糜子, 矮秆突变体“819”, 遗传分析, 主成分

Abstract: In order to uncover the genetic characteristics of dwarf gene from a dwarf mutant “819”, and provide theoretical basis for the subsequent locating of dwarf mutant gene ten traits' including plant height, ear length, neck length, tiller number, stem node number, length of second panicle branch 1, length of second panicle branch 2, length of second panicle branch 3 and so on were investigated for Panicum miliaceum L. crossing parents dwarf mutant “819”, high-stem material J12 and their 674 F₂ individuals, and further variance analysis, cartesian inspection, correlation and regression analysis, principal component analysis were performed for these traits. The results showed that dwarf trait of dwarf mutant “819” was a recessive trait controlled by a single gene. A significantly positive correlation was found between plant height and ear length, tiller number, stem node number, secondary branch length 1, secondary branch length 2, secondary branch length 3, secondary branch distance 1 (P<0.01), and a significant positive correlation was found between plant height and secondary branch spacing 2 (P<0.05). The regression equation of plant height (Y) and other agronomic traits was as follow: Y=-18.446+1.491X₁+1.222X₂+6.827X₄+1.319X₇+0.746X₈, and the fitting degree of which was 0.811. The regression equation reached significant level (P<0.01) and could be used to predict the plant height. Three principal components were obtained by principal component analysis for 10 characters of F₂ population, which accounted for 72.656% of the cumulative contribution rate. These three principal components were named as length factor, stem node number factor and branch spacing factor respectively. The results above provided basis for locating dwarf gene and evaluating, selecting of dwarf offsprings.

Key words: Panicum miliaceum L., dwarf mutant 819, genetic analysis, principal component

中图分类号:

S516

贾小平, 王振山, 朱学海, 杨德智, 寇淑君, 刘星星. 糜子矮秆突变体“819”矮秆基因的遗传学分析[J]. 浙江农业学报, 2020, 32(1): 20-27.

JIA Xiaoping, WANG Zhenshan, ZHU Xuehai, YANG Dezhi, KOU Shujun, LIU Xingxing. Genetic analysis of dwarf gene for dwarf mutant“819” in Panicum miliaceum L.[J]. , 2020, 32(1): 20-27.

参考文献

[1] 李文强. 水稻矮秆基因d62和光叶基因gl1的图位克隆及功能研究[D]. 杭州: 浙江大学, 2010.
LI W Q.Map-based cloning and functional analysis of the dwarf gene d62 and glabrous gene gl1 in rice[D]. Hangzhou: Zhejiang University, 2010.(in Chinese with English abstract)
[2] 王益军, 苗楠, 施亚婷, 等. 一份玉米显性矮杆突变体的遗传分析[J]. 华北农学报, 2010, 25(5): 90-93.
WANG Y J, MIAO N, SHI Y T, et al.Genetic analysis of a dominant dwarf mutant in maize[J]. Acta Agriculturae Boreali-Sinica, 2010, 25(5): 90-93.(in Chinese with English abstract)
[3] 卢永根, 曾世雄, 李镇邦, 等. 我国早籼稻矮生性基因源的表型表现和遗传传递的研究[J]. 遗传学报, 1979, 6(3): 311-321.
LU Y G, ZENG S X, LI Z B, et al.A study on the phenotypic expression and genetic transmission of dwarf-gene sources of early hsien rice in China[J]. Acta Genetica Sinica, 1979, 6(3): 311-321.(in Chinese with English abstract)
[4] 顾铭洪, 朱立宏. 粳稻矮秆基因遗传分析[J]. 遗传, 1981, 3(3): 20-23.
GU M H, ZHU L H.Genetical analysis of se-midwarf genes in Oryza sativa subsp. keng[J]. Hereditas, 1981, 3(3): 20-23. (in Chinese with English abstract)
[5] 熊振民, 闵绍楷, 程式华. 我国水稻矮源的研究与利用[J]. 水稻文摘, 1988, 7(4): 1-5.
XIONG Z M, MIN S K, CHENG S H.Research and utilization of rice dwarf sources in China[J]. Rice Abstracts, 1988, 7(4): 1-5. (in Chinese with English abstract)
[6] 李欣, 朱立宏. 粳稻矮生性的遗传研究[J]. 南京农业大学学报, 1982(3): 12-25.
LI X, ZHU L H.Genetic study of dwarfism in Oryza sativa subsp. keng[J]. Journal of Nanjing Agricultural University, 1982(3): 12-25.(in Chinese with English abstract)
[7] 袁倩倩. 小麦苗期性状和穗部性状的QTL定位及杂种优势的遗传分析[D]. 泰安: 山东农业大学, 2012.
YUAN Q Q.Genetic analysis of QTL mapping and heterosis for seeding traits and spike related traits[D]. Tai'an: Shandong Agricultural University, 2012.(in Chinese with English abstract)
[8] 张晓科, 王辉. 小麦矮源的研究和利用现状[J]. 麦类作物学报, 1996, 4: 10-12.
ZHANG X K, WANG H.Research and utilization status of wheat dwarf source[J]. Journal of Triticeae Crops, 1996, 4: 10-12.(in Chinese with English abstract)
[9] HU X Y, WANG J F, LU P, et al.Assessment of genetic diversity in broomcorn millet (Panicum miliaceum L.) using SSR markers[J]. Journal of Genetics and Genomics, 2009, 36(8): 491-500.
[10] HUNT H V, CAMPANA M G, LAWES M C, et al.Genetic diversity and phylogeography of broomcorn millet (Panicum miliaceum L.) across Eurasia[J]. Molecular Ecology, 2011, 20(22): 4756-4771.
[11] 张博, 贾小平, 杨德智, 等. 糜子矮秆突变体778农艺性状调查及其对GA的敏感性分析[J]. 浙江农业学报, 2019, 31(5): 688-694.
ZHANG B, JIA X P, YANG D Z, et al.Investigation on agronomic characters of dwarf mutant in Panicum miliaceuml and analysis of its sensitivity to GA[J]. Acta Agriculturae Zhejiangensis, 2019, 31(5): 688-694.(in Chinese with English abstract)
[12] 李延玲, 白晓倩, 于澎湃, 等. 高粱株型性状数量遗传分析[J]. 华北农学报, 2018, 33(1): 143-149.
LI Y L, BAI X Q, YU P P, et al.Quantitative genetic analysis of Sorghum plant type characters[J]. Acta Agriculturae Boreali-Sinica, 2018, 33(1): 143-149.(in Chinese with English abstract)
[13] 潘晶. 大豆矮杆突变体的初步遗传分析及大豆老化种子引发处理研究[D]. 聊城: 聊城大学, 2016.
PAN J.Preliminary genetic analysis of soybean dwarf mutant and priming treatment of soybean aging seeds [D]. Liaocheng: Liaocheng University, 2016. (in Chinese with English abstract)
[14] 高奋明. 一个水稻半矮杆突变体的遗传分析与基因定位[D]. 雅安: 四川农业大学, 2005.
GAO F M.Genetic analysis and gene mapping of a semi-dwarf mutant in rice[D]. Ya'an: Sichuan Agricultural University, 2005.(in Chinese with English abstract)
[15] 韩玉翠, 朱学海, 相金英, 等. 糜子育成新品种的鉴定与评价[J]. 河北农业科学, 2015, 19(3): 12-17.
HAN Y C, ZHU X H, XIANG J Y, et al.Identification and evaluation of newly bred proso millet(Panicum miliaceum) varieties in China[J]. Journal of Hebei Agricultural Sciences, 2015, 19(3): 12-17.(in Chinese with English abstract)
[16] 陆维忠, 赵寅槐, 冯晓棠, 等. 小麦“矮变一号”的矮秆性遗传研究[J]. 作物学报, 1985, 11(1): 39-46.
LU W Z, ZHAO Y H, FENG X T, et al.Studies on dwarfness inheritance of Aibian No.1 wheat[J]. Acta Agronomica Sinica, 1985, 11(1): 39-46.(in Chinese with English abstract)
[17] 薛建峰, 汤永荣, 查仁明, 等. 2个水稻矮杆突变体的遗传分析[J]. 中国农学通报, 2011, 27(27): 60-64.
XUE J F, TANG Y R, ZHA R M, et al.Genetic analysis of two dwarf mutants of rice[J]. Chinese Agricultural Science Bulletin, 2011, 27(27): 60-64. (in Chinese with English abstract)
[18] 李文滨, 王金陵. 利用矮杆基因改良大豆种间杂种的蔓生性[J]. 东北农学院学报, 1987, 18(4): 301-310.
LI W B, WANG J L.A study on overcoming the viny habit of Glycine max × G. soja hybrids by means of using g. max parents with dwarf gene (a preliminary report)[J]. Journal of Northeast Agricultural College, 1987, 18(4): 301-310.(in Chinese with English abstract)
[19] 江婷婷. 一份玉米矮杆新材料的创制及鉴定[D]. 雅安: 四川农业大学, 2016.
JIANG T T.Creation and identification of a new dwarf maize[D]. Ya'an: Sichuan Agricultural University, 2016.(in Chinese with English abstract)
[20] 黄英杰, 林润年. 黍子主要农艺性状对产量影响的效应分析[J]. 吉林农业科学, 1993, 18(3): 31-33.
HUANG Y J, LIN R N.Effects of main agronomic characters of millet on yield[J]. Journal of Jilin Agricultural Sciences, 1993, 18(3): 31-33.(in Chinese with English abstract)

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糜子矮秆突变体“819”矮秆基因的遗传学分析

Genetic analysis of dwarf gene for dwarf mutant“819” in Panicum miliaceum L.

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