基于DNA G-四链体的农兽药残留与重金属离子快速检测研究进展

doi:10.3969/j.issn.1004-1524.2021.09.21

浙江农业学报 ›› 2021, Vol. 33 ›› Issue (9): 1770-1778.DOI: 10.3969/j.issn.1004-1524.2021.09.21

基于DNA G-四链体的农兽药残留与重金属离子快速检测研究进展

孙丽萍¹^,²(), 白琳琳²^,³, 干雅婷¹, 陈雪雲²^,³, 王柳², 张宜明¹^,^*(), 何开雨²^,^*(), 徐霞红²

1.浙江农林大学农业与食品科学学院,浙江杭州 311300
2.浙江省农业科学院农产品质量安全与营养研究所,浙江杭州 310021
3.福州大学生物科学与工程学院,福建福州 350108

收稿日期:2020-08-10 出版日期:2021-09-25 发布日期:2021-10-09
作者简介:何开雨,E-mail: hekaiyu@zaas.ac.cn
* 张宜明,E-mail: zym7307@zju.edu.cn;
孙丽萍(1995—),女,山东临沂人,硕士研究生,研究方向为食品中有毒有害物质的快速检测。E-mail: 305104261@qq.com
通讯作者: 张宜明,何开雨
基金资助:
国家自然科学基金(31701707);浙江省基础公益研究计划(LGF18C200006);浙江省基础公益研究计划(LGN19C200021)

Research progress of rapid detection of pesticides and veterinary drugs residues and heavy metal ions based on DNA G-quadruplex

SUN Liping¹^,²(), BAI Linlin²^,³, GAN Yating¹, CHEN Xueyun²^,³, WANG Liu², ZHANG Yiming¹^,^*(), HE Kaiyu²^,^*(), XU Xiahong²

1. College of Agriculture and Food Science, Zhejiang A&F University, Hangzhou 311300, China
2. Institute of Quality Safety and Nutrition of Agri-Products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
3. College of Biological Science and Engineering, Fuzhou University, Fuzhou 350108, China

Received:2020-08-10 Online:2021-09-25 Published:2021-10-09
Contact: ZHANG Yiming,HE Kaiyu

摘要/Abstract

摘要：

DNA的结构具有多样性,除经典的双螺旋结构外,近年来的研究发现和组装获得了更为多样的DNA结构类型,如三螺旋结构、发夹结构、i-motif结构、G-四链体等非传统结构。这些特殊结构具有一定的功能,例如,G-四链体可与氯高铁血红素结合形成具有辣根过氧化物酶活性的复合物,也可增强某些染料分子的荧光强度。此外,G-四链体的形成与稳定性可控、可设计,是构建化学生物传感的良好材料。以一些典型的研究工作为例,本文对近年来G-四链体在农产品中农兽药残留及重金属离子快速检测中的应用进行了总结,并对其应用前景进行展望。

关键词: G-四链体, 农药, 兽药, 重金属离子, 比色法, 荧光法, 快速检测

Abstract:

The conformation of DNA are diverse. In addition to the well-known double helix,a number of unconventional structures have been discovered and assembled, such as DNA triple helix,DNA hairpin structure,DNA i-motif, DNA G-quadruplex, and so on. These special DNA structures have some certain functions, for example, DNA G-quadruplexes can bind with hemin and significantly improve the intrinsic peroxidase activity of hemin. Besides this function, they also enhance the fluorescence intensity of some organic dyes. Further more, the formation and stability of G-quadruplexes are programmable and controllable, and they are excellent materials for the construction of chemosensors and biosensors. Taking some typical research works as examples, this paper summarized the application of G-quadruplexes in the rapid detection of pesticide residues, veterinary residues and heavy metals, and the prospective research and application are also discussed.

Key words: G-quadruplex, pesticides, veterinary drugs, heavy metal ions, colorimetric, fluorescence, rapid detection

中图分类号:

S481⁺.8

孙丽萍, 白琳琳, 干雅婷, 陈雪雲, 王柳, 张宜明, 何开雨, 徐霞红. 基于DNA G-四链体的农兽药残留与重金属离子快速检测研究进展[J]. 浙江农业学报, 2021, 33(9): 1770-1778.

SUN Liping, BAI Linlin, GAN Yating, CHEN Xueyun, WANG Liu, ZHANG Yiming, HE Kaiyu, XU Xiahong. Research progress of rapid detection of pesticides and veterinary drugs residues and heavy metal ions based on DNA G-quadruplex[J]. Acta Agriculturae Zhejiangensis, 2021, 33(9): 1770-1778.

图/表 5

图1 G-四分体及G-四链体 A,G-四分体结构;B,分子内折叠形成G-四链体(反平行结构);C,双分子折叠形成G-四链体(反平行结构);D,四分子折叠形成G-四链体(平行结构)。

Fig.1 G-tetrad and G-quadruplexes A, G-tetrad structure; B, Monomolecular folded G-quadruplex (Antiparallel structure); C, Bimolecular folded G-quadruplex (Antiparallel structure); D, Tetramolecular folded G-quadruplex (Parallel structure).

图2 染料分子结构式 A,卟啉[33];B,硫磺素T[34];C,噻唑橙[35];D,N-甲基吗啡啉[36]。

Fig.2 Molecular structural formula of dyes A, Protoporphyrin IX[33]; B, Thioflavin T[34]; C, Thiazole Orange[35]; D, N-methylmorpholine[36].

图3 基于G-四链体传感快速检测农产品中兽药残留 A,基于DNA G-四链体和三螺旋结构快速检测四环素[39];B,利用DNA G-四链体构建SERS传感平台快速检测孔雀石绿[40]。

Fig.3 Rapid detection of veterinary drug residues in agricultural products based on G-quadruplex sensors A, Scheme of tetracycline rapid detection based on DNA G-quadruplex and triple helix[39]; B, Scheme of G-quadruplex based SERS sensing platform for the rapid detection of malachite green[40].

图4 基于G-四链体传感快速检测农产品中农药残留 A,利用乙醇胺适配体形成的G-四链体快速检测乙醇胺原理图[42];B,基于DNA 适配体和G-四链体免标记无酶荧光生物传感快速检测水胺硫磷[43];C,基于DNA三螺旋结构分子开关和G-四链体荧光生物传感快速检测啶虫脒[44]。

Fig.4 Rapid detection of pesticide residues in agricultural products based on G-quadruplex sensors A, The rapid detection of ethanolamine based on G-quadruplex formed by ethanolamine aptamer[42]; B, The label-free and enzyme-free fluorescent biosensor for the rapid detection of ICP[43]; C, Fluorescence biosensor for the detection of acetamiprid based on DNA triple helix molecular switch and G-quadruplex[44].

图5 基于G-四链体传感快速检测农产品中重金属离子 A,基于DNA G-四链体的Hg2+免标记传感器[45];B,G-四链体-hemin催化放大电化学信号灵敏检测Pb2+[46];C,基于G-四链体的Ag+快速检测[47]。

Fig.5 Rapid detection of heavy metal ions in agricultural products based on G-quadruplex sensors A, Scheme of the label-free Hg2+ sensor based on G-quadruplex[45]; B, DNA G-quadruplex-hemin amplified signal for the sensitive electrochemical detection of Pb2+[46]; C, DNA G-quadruplex based Ag+ biosensor[47].

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基于DNA G-四链体的农兽药残留与重金属离子快速检测研究进展

Research progress of rapid detection of pesticides and veterinary drugs residues and heavy metal ions based on DNA G-quadruplex

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