外源钙浸种对小麦防御酶和麦二叉蚜体内解毒酶活性的影响

doi:10.3969/j.issn.1004-1524.2021.12.14

浙江农业学报 ›› 2021, Vol. 33 ›› Issue (12): 2339-2347.DOI: 10.3969/j.issn.1004-1524.2021.12.14

外源钙浸种对小麦防御酶和麦二叉蚜体内解毒酶活性的影响

王骏¹^,²(), 吴小保¹^,², 宋佳¹^,², 邓倩倩¹^,², 曾广¹^,², 任明见³, 叶茂¹^,²^,^*()

1.贵州大学昆虫研究所,贵州贵阳 550025
2.贵州省山地农业病虫害重点实验室,贵州贵阳 550025
3.国家小麦改良中心贵州分中心,贵州贵阳 550025

收稿日期:2021-03-10 出版日期:2021-12-25 发布日期:2022-01-10
通讯作者: 叶茂
作者简介:* 叶茂,E-mail: Mao_Ye@yeah.net
王骏(1995—),男,贵州遵义人,硕士研究生,主要从事作物抗性与化学生态研究。E-mail: wangjun951030@163.com
基金资助:
国家自然科学基金(31701801);国家自然科学基金(32060621);贵州省科技计划(〔2016〕1043);贵州省科技计划(〔2017〕5788);贵州省科技计划(〔2017〕7276);贵州省科技计划(〔2019〕4246)

Effect of seed soaking with exogenous calcium on activities of defense enzymes in wheat and detoxifying enzymes in Schizaphis graminum (Rondani)

WANG Jun¹^,²(), WU Xiaobao¹^,², SONG Jia¹^,², DENG Qianqian¹^,², ZENG Guang¹^,², REN Mingjian³, YE Mao¹^,²^,^*()

1. Institute of Entomology, Guizhou University, Guiyang 550025, China
2. Guizhou Provincial Key Laboratory of Agricultural Pest Management of the Mountainous Regions, Guiyang 550025, China
3. Guizhou Branch of National Wheat Improvement Center, Guiyang 550025, China

Received:2021-03-10 Online:2021-12-25 Published:2022-01-10
Contact: YE Mao

摘要/Abstract

摘要：

为了研究外源钙介导下小麦(Triticum aestivum L.)与麦二叉蚜[Schizaphis graminum(Rondani)]的互作关系,用浸种法对小麦进行外源氯化钙处理,以蒸馏水浸种处理为对照,检测小麦植株遭麦二叉蚜取食0、24、48、72 h时叶片中过氧化物酶(POD)、多酚氧化酶(PPO)、苯丙氨酸解氨酶(PAL)和β-1,3葡聚糖酶(β-1,3-GA)活性的变化,及麦二叉蚜取食不同处理小麦植株0、24、48、72 h时体内谷胱甘肽巯基转移酶(GSTs)、羧酸酯酶(CarE)和细胞色素P450(CYP450)活性的变化。结果发现,麦二叉蚜取食显著(P<0.05)诱导了小麦叶片POD、PAL和β-1,3-GA活性,但对PPO活性无显著诱导。氯化钙浸种处理进一步显著(P<0.05)增强了POD、PAL和β-1,3-GA的活性,且PPO活性也显著(P<0.05)高于未经氯化钙处理的小麦植株。麦二叉蚜取食氯化钙浸种处理的小麦植株后,其体内解毒酶GSTs、CarE和CYP450的活性显著(P<0.05)高于取食对照植株的麦二叉蚜。这说明,外源钙对小麦叶片中的防御酶活性有明显诱导作用,取食经外源钙处理的小麦叶片后,麦二叉蚜也相应提高了其体内解毒酶的活性,以应对小麦植株增强的抗性。

关键词: 小麦, 麦二叉蚜, 外源钙, 防御酶, 解毒酶

Abstract:

In order to study the interaction mediated by exogenous calcium between Triticum aestivum L. and Schizaphis graminum(Rondani), wheat seeds were soaked with calcium chloride solution (CaCl₂ treatment), while seeds soaked with distilled water were taken as control (CK). The changes of peroxidase (POD), polyphenol oxidase (PPO), phenylalanine ammonia lyase (PAL) and β-1,3-glucanase (β-1,3-GA) activity in wheat leaves were detected at 0, 24, 48, 72 h after aphids infestation. Meanwhile, glutathione S-transferase (GSTs), carboxylesterase (CarE) and cytochrome P450 (CYP450) activities in aphids fed on wheat plant for 0, 24, 48, 72 h were tested as well. It was found that the aphids infestation significantly (P<0.05) induced POD, PAL and β-1,3-GA activities in wheat leaves, but PPO activity did not increase, while CaCl₂ treatment further significantly (P<0.05) enhanced activities of POD, PAL and β-1,3-GA. Besides,PPO activity in wheat plants was significantly (P<0.05) higher under CaCl₂ treatment than that under CK. Meanwhile, the activities of detoxification enzymes (GSTs, CarE and CYP450) in aphids fed on CaCl₂-treated plants were significantly (P<0.05) increased as compared with those fed on control plants. Collectively, the present results indicated that the exogenous calcium could induce the activities of defensive enzymes in wheat plants, while aphids increased their detoxification enzymes activities to adapt the enhanced defense of wheat.

Key words: wheat, Schizaphis graminum(Rondani), exogenous calcium, defense enzyme, detoxification enzyme

中图分类号:

S435
S512

王骏, 吴小保, 宋佳, 邓倩倩, 曾广, 任明见, 叶茂. 外源钙浸种对小麦防御酶和麦二叉蚜体内解毒酶活性的影响[J]. 浙江农业学报, 2021, 33(12): 2339-2347.

WANG Jun, WU Xiaobao, SONG Jia, DENG Qianqian, ZENG Guang, REN Mingjian, YE Mao. Effect of seed soaking with exogenous calcium on activities of defense enzymes in wheat and detoxifying enzymes in Schizaphis graminum (Rondani)[J]. Acta Agriculturae Zhejiangensis, 2021, 33(12): 2339-2347.

图/表 7

图1 不同处理对小麦叶片过氧化物酶(POD)活性的影响柱上无相同字母的表示同一处理不同时间酶活性差异显著(P<0.05),标“*”的表示同一取食时间不同处理间酶活性差异显著(P<0.05)。下同。

Fig. 1 Effect of different treatments on peroxidase (POD) activity of wheat leaves Bars marked without the same letters indicated significant(P<0.05) differences of enzymatic activity under the same treatment within different feeding time; “*”indicated significant (P<0.05) difference of enzymatic activity between treatments at the same feeding time.The same as below.

图2 不同处理对小麦叶片多酚氧化酶(PPO)活性的影响

Fig.2 Effect of different treatments on polyphenol oxidase (PPO) activity of wheat leaves

图3 不同处理对小麦叶片苯丙氨酸解氨酶(PAL)活性的影响

Fig.3 Effect of different treatments on phenylalanine ammonia lyase (PAL) activity of wheat leaves

图4 不同处理对小麦叶片β-1,3葡聚糖酶(β-1,3-GA)活性的影响

Fig.4 Effect of different treatments on β-1,3-glucanase (β-1,3-GA) activity of wheat leaves

图5 不同处理对麦二叉蚜体内谷胱甘肽巯基转移酶(GSTs)活性的影响

Fig.5 Effect of different treatments on glutathione S-transferase (GSTs) activity of Schizaphis graminum(Rondani)

图6 不同处理对麦二叉蚜体内羧酸酯酶(CarE)活性的影响

Fig.6 Effect of different treatments on carboxylesterase (CarE) activity of Schizaphis graminum(Rondani)

图7 不同处理对麦二叉蚜体内细胞色素P450(CYP450)活性的影响

Fig.7 Effect of different treatments on cytochrome P450 activity of Schizaphis graminum(Rondani)

参考文献 48

[1]	李翠平, 秦保平, 李亚静, 等. 减少灌水量对强筋小麦花后干物质和氮素积累、转运及产量的影响[J]. 麦类作物学报, 2020, 40(8): 964-971.
	LI C P, QIN B P, LI Y J, et al. Effect of reducing irrigation amount on the accumulation and translocation of dry matter and nitrogen and grain yield after flowering of strong gluten wheat[J]. Journal of Triticeae Crops, 2020, 40(8): 964-971.(in Chinese with English abstract)
[2]	徐民民, 黄莹, 李波, 等. 生物炭对小麦根际和根内微生物群落结构的影响[J]. 浙江农业学报, 2021, 33(3): 516-525.
	XU M M, HUANG Y, LI B, et al. Effect of biochar on wheat root-associated microbial community structures[J]. Acta Agriculturae Zhejiangensis, 2021, 33(3): 516-525.(in Chinese with English abstract)
[3]	REDDY S K, WENG Y Q, RUDD J C, et al. Transcriptomics of induced defense responses to greenbug aphid feeding in near isogenic wheat lines[J]. Plant Science, 2013, 212: 26-36. DOI URL
[4]	禹海鑫, 叶文丰, 孙民琴, 等. 植物与植食性昆虫防御与反防御的三个层次[J]. 生态学杂志, 2015, 34(1): 256-262.
	YU H X, YE W F, SUN M Q, et al. Three levels of defense and anti-defense responses between host plants and herbivorous insects[J]. Chinese Journal of Ecology, 2015, 34(1): 256-262.(in Chinese with English abstract)
[5]	王小菲, 高文强, 刘建锋, 等. 植物防御策略及其环境驱动机制[J]. 生态学杂志, 2015, 34(12): 3542-3552.
	WANG X F, GAO W Q, LIU J F, et al. Plant defensive strategies and environment-driven mechanisms[J]. Chinese Journal of Ecology, 2015, 34(12): 3542-3552.(in Chinese with English abstract)
[6]	张苏芳, 孔祥波, 王鸿斌, 等. 植物对昆虫不同防御类型及内在联系[J]. 应用昆虫学报, 2013, 50(5): 1428-1437.
	ZHANG S F, KONG X B, WANG H B, et al. Different strategies of plant resistance to insects and their interactions[J]. Chinese Journal of Applied Entomology, 2013, 50(5): 1428-1437.(in Chinese with English abstract)
[7]	ZENG G, ZHI J R, YE M, et al. Inductive effects of exogenous calcium on the defense of kidney bean plants against Frankliniella occidentalis(Thysanoptera: Thripidae)[J]. Arthropod-Plant Interactions, 2020, 14(4): 473-480. DOI URL
[8]	郭祖国, 王梦馨, 崔林, 等. 6种防御酶调控植物体应答虫害胁迫机制的研究进展[J]. 应用生态学报, 2018, 29(12): 4248-4258.
	GUO Z G, WANG M X, CUI L, et al. Research progress on the underlying mechanisms of plant defense enzymes in response to pest stress[J]. Chinese Journal of Applied Ecology, 2018, 29(12): 4248-4258. (in Chinese with English abstract)
[9]	HONG J K, HWANG B K. Functional characterization of PR-1 protein, β-1, 3-glucanase and chitinase genes during defense response to biotic and abiotic stresses in Capsicum annuum[J]. The Plant Pathology Journal, 2005, 21(3): 195-206. DOI URL
[10]	张廷伟, 刘长仲. 禾谷缢管蚜对三个小麦品种幼苗保护酶的影响[J]. 植物保护, 2011, 37(4): 72-75.
	ZHANG T W, LIU C Z. Influences of Rhopalosiphum padi on oxidases of three wheat seedlings[J]. Plant Protection, 2011, 37(4): 72-75.(in Chinese with English abstract)
[11]	XU Y, GUO H, GENG G D, et al. Changes in defense-related enzymes and phenolics in resistant and susceptible common wheat cultivars under aphid stress[J]. Acta Physiologiae Plantarum, 2021, 43(2): 1-9. DOI URL
[12]	孙多鑫, 尚勋武, 师桂英, 等. 四种酶与春小麦抗蚜性的相关性研究[J]. 甘肃农业大学学报, 2006, 41(5): 45-49.
	SUN D X, SHANG X W, SHI G Y, et al. Effect of four kinds of enzymes on resistance to aphide of spring wheat[J]. Journal of Gansu Agricultural University, 2006, 41(5): 45-49.(in Chinese with English abstract)
[13]	ZÜST T, AGRAWAL A A. Mechanisms and evolution of plant resistance to aphids[J]. Nature Plants, 2016, 2: 15206. DOI URL
[14]	彭露, 严盈, 刘万学, 等. 植食性昆虫对植物的反防御机制[J]. 昆虫学报, 2010, 53(5): 572-580.
	PENG L, YAN Y, LIU W X, et al. Counter-defense mechanisms of phytophagous insects towards plant defense[J]. Acta Entomologica Sinica, 2010, 53(5): 572-580.(in Chinese with English abstract)
[15]	李顺欣, 郅军锐, 杨广明, 等. 外源茉莉酸诱导的菜豆叶片生化抗性及其对西花蓟马体内保护酶和解毒酶活性的影响[J]. 应用生态学报, 2017, 28(9): 2975-2983.
	LI S X, ZHI J R, YANG G M, et al. Resistance of bean leaves induced by exogenous jasmonic acid and its effects on activities of protective and detoxification enzymes in Frankliniella occidentalis[J]. Chinese Journal of Applied Ecology, 2017, 28(9): 2975-2983.(in Chinese with English abstract)
[16]	李定银, 郅军锐, 张涛, 等. 乙基多杀菌素和乙虫腈对西花蓟马解毒酶和乙酰胆碱酯酶活性的影响[J]. 应用昆虫学报, 2020, 57(6): 1385-1393.
	LI D Y, ZHI J R, ZHANG T, et al. Effects of spinetoram and ethiprole on detoxification enzyme and acetylcholin esterase activity in Frankliniella occidentalis (Pergande)[J]. Chinese Journal of Applied Entomology, 2020, 57(6): 1385-1393.(in Chinese with English abstract)
[17]	CAI Q N, ZHANG Q W, CHEO M. Contribution of indole alkaloids to Sitobion avenae(F.) resistance in wheat[J]. Journal of Applied Entomology, 2004, 128(8): 517-521. DOI URL
[18]	LOAYZA-MURO R, FIGUEROA C C, NIEMEYER H M. Effect of two wheat cultivars differing in hydroxamic acid concentration on detoxification metabolism in the aphid Sitobion avenae[J]. Journal of Chemical Ecology, 2000, 26(12): 2725-2736. DOI URL
[19]	SHEN Z G, WANG J L, GUAN H Y. Effect of aluminium and calcium on growth of wheat seedlings and germination of seeds[J]. Journal of Plant Nutrition, 1993, 16(11): 2135-2148. DOI URL
[20]	YÜCEL N C, HEYBET E H. Salicylic acid and calcium treatments improves wheat vigor, lipids and phenolics under high salinity[J]. Acta Chimica Slovenica, 2016, 63: 738-746.
[21]	ALBORN H T, TURLINGS T C J, JONES T H, et al. An elicitor of plant volatiles from beet armyworm oral secretion[J]. Science, 1997, 276(5314): 945-949. DOI URL
[22]	ALBORN H T, HANSEN T V, JONES T H, et al. Disulfooxy fatty acids from the American bird grasshopper Schistocerca americana, elicitors of plant volatiles[J]. Proceedings of the National Academy of Sciences of the United States of America, 2007, 104(32): 12976-12981.
[23]	SCHMELZ E A, LECLERE S, CARROLL M J, et al. Cowpea chloroplastic ATP synthase is the source of multiple plant defense elicitors during insect herbivory[J]. Plant Physiology, 2007, 144(2): 793-805. DOI URL
[24]	WINTER T R, BORKOWSKI L, ZEIER J, et al. Heavy metal stress can prime for herbivore-induced plant volatile emission[J]. Plant, Cell & Environment, 2012, 35(7): 1287-1298.
[25]	王祎, 张月玲, 苏建伟, 等. 施钾提高蚜害诱导的小麦茉莉酸含量和叶片相关防御酶活性[J]. 生态学报, 2014, 34(10): 2539-2547.
	WANG Y, ZHANG Y L, SU J W, et al. Potassium application for increased jasmonic acid content and defense enzyme activities of wheat leaves infested by aphids[J]. Acta Ecologica Sinica, 2014, 34(10): 2539-2547.(in Chinese with English abstract)
[26]	卢伟, 侯茂林, 文吉辉, 等. 寄主钾营养对烟粉虱发育、存活和寄主选择的影响[J]. 昆虫学报, 2007, 50(3): 253-258.
	LU W, HOU M L, WEN J H, et al. Effects of host potassium nutrition on development, survival and host selection of Bemisia tabaci(Gennadius)[J]. Acta Entomologica Sinica, 2007, 50(3): 253-258.(in Chinese with English abstract)
[27]	YE M, SONG Y Y, LONG J, et al. Priming of jasmonate-mediated antiherbivore defense responses in rice by silicon[J]. Proceedings of the National Academy of Sciences of the United States of America, 2013, 110(38): E3631-E3639.
[28]	WANG J, XUE R R, JU X Y, et al. Silicon-mediated multiple interactions: simultaneous induction of rice defense and inhibition of larval performance and insecticide tolerance of Chilo suppressalis by sodium silicate[J]. Ecology and Evolution, 2020, 10(11): 4816-4827. DOI URL
[29]	ALLEN G J, CHU S P, HARRINGTON C L, et al. A defined range of guard cell calcium oscillation parameters encodes stomatal movements[J]. Nature, 2001, 411(6841): 1053-1057. DOI URL
[30]	TOYOTA M, SPENCER D, SAWAI-TOYOTA S, et al. Glutamate triggers long-distance, calcium-based plant defense signaling[J]. Science, 2018, 361(6407): 1112-1115. DOI URL
[31]	袁庆华, 桂枝, 张文淑. 苜蓿抗感褐斑病品种内超氧化物歧化酶、过氧化物酶和多酚氧化酶活性的比较[J]. 草业学报, 2002, 11(2): 100-104.
	YUAN Q H, GUI Z, ZHANG W S. Comparison of the activities of SOD, POD and PPO within alfalfa cultivars resistant and susceptible to alfalfa common leaf[J]. Acta Pratacultural Science, 2002, 11(2): 100-104.(in Chinese with English abstract)
[32]	吴文华, 潘瑞炽. 茉莉酸甲酯对水稻幼苗叶片中碳水化合物含量及苯丙氨酸解氨酶和多酚氧化酶活性的影响(简报)[J]. 植物生理学通讯, 1997, 33(3): 178-180.
	WU W H, PAN R C. Effect of Ja-Me on carbohydrate contents and activities of phenylalanine ammonia-lyase and polyphenol oxidase in leaves of rice seedlings[J]. Plant Physiology Communications, 1997, 33(3): 178-180.(in Chinese)
[33]	BRADFORD M M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding[J]. Analytical Biochemistry, 1976, 72(1/2): 248-254. DOI URL
[34]	HARRINGTON R, CLARK S J, WELHAM S J, et al. Environmental change and the phenology of European aphids[J]. Global Change Biology, 2007, 13(8): 1550-1564. DOI URL
[35]	GIROUSSE C, MOULIA B, SILK W, et al. Aphid infestation causes different changes in carbon and nitrogen allocation in alfalfa stems as well as different inhibitions of longitudinal and radial expansion[J]. Plant Physiology, 2005, 137(4): 1474-1484. DOI URL
[36]	ZHAO Y, TU K, SHAO X F, et al. Effects of the yeast Pichia guilliermondii against Rhizopus nigricans on tomato fruit[J]. Postharvest Biology and Technology, 2008, 49(1): 113-120. DOI URL
[37]	李天来, 张亢亢, 余朝阁, 等. 外源钙和茉莉酸甲酯诱导番茄植株抗灰霉病研究[J]. 西北植物学报, 2012, 32(3): 505-510.
	LI T L, ZHANG K K, YU Z G, et al. Research of exogenous calcium and methyl jasmonate on resistance to Botrytis cinerea in tomato[J]. Acta Botanica Boreali-Occidentalia Sinica, 2012, 32(3): 505-510.(in Chinese with English abstract)
[38]	宋佳, 王骏, 叶茂. 茉莉酸甲酯诱导邻近番茄植株抗斜纹夜蛾的浓度和距离效应[J]. 生态学报, 2020, 40(15): 5433-5440.
	SONG J, WANG J, YE M. Concentration and distance effects of airborne methyl jasmonate on neighboring tomato plants’ resistance against Spodoptera litura[J]. Acta Ecologica Sinica, 2020, 40(15): 5433-5440.(in Chinese with English abstract)
[39]	SU J, TU K, CHENG L, et al. Wound-induced H₂O₂ and resistance to Botrytis cinerea decline with the ripening of apple fruit[J]. Postharvest Biology and Technology, 2011, 62(1): 64-70. DOI URL
[40]	DUBEY N K, GOEL R, RANJAN A, et al. Comparative transcriptome analysis of Gossypium hirsutum L. in response to sap sucking insects: aphid and whitefly[J]. BMC Genomics, 2013, 14: 241. DOI URL
[41]	ZHAO L Y, CHEN J L, CHENG D F, et al. Biochemical and molecular characterizations of Sitobion avenae-induced wheat defense responses[J]. Crop Protection, 2009, 28(5): 435-442. DOI URL
[42]	郭玉莲, 陶波, 郑铁军, 等. 植物谷胱甘肽S-转移酶(GSTs)及除草剂解毒剂的诱导作用[J]. 东北农业大学学报, 2008, 39(7): 136-139.
	GUO Y L, TAO B, ZHENG T J, et al. Inducement action of plant GSTs and herbicides antidotes[J]. Journal of Northeast Agricultural University, 2008, 39(7): 136-139.(in Chinese with English abstract)
[43]	汤方, 李丽, 高希武. 植物次生物质和杀虫剂对分月扇舟蛾谷胱甘肽S-转移酶的抑制作用[J]. 应用昆虫学报, 2012, 49(6): 1513-1518.
	TANG F, LI L, GAO X W. Inhibition of glutathione S-transferase activity by insecticides and allelochemicals in Clostera anastomosis[J]. Chinese Journal of Applied Entomology, 2012, 49(6): 1513-1518.(in Chinese with English abstract)
[44]	LIU S, GONG Z J, RAO X J, et al. Identification of putative carboxylesterase and glutathione S-transferase genes from the antennae of the Chilo suppressalis(Lepidoptera: Pyralidae)[J]. Journal of Insect Science, 2015, 15(1): 103. DOI URL
[45]	LU K, SONG Y Y, ZENG R S. The role of cytochrome P450-mediated detoxification in insect adaptation to xenobiotics[J]. Current Opinion in Insect Science, 2021, 43: 103-107. DOI URL
[46]	LAN H, ZHANG Z F, WU J, et al. Performance and transcriptomic response of the English grain aphid, Sitobion avenae, feeding on resistant and susceptible wheat cultivars[J]. Journal of Integrative Agriculture, 2021, 20(1): 178-190. DOI
[47]	牟少飞, 梁沛, 高希武. 槲皮素对B型烟粉虱羧酸酯酶和谷胱甘肽S-转移酶活性的影响[J]. 昆虫知识, 2006, 43(4): 491-495.
	MU S F, LIANG P, GAO X W. Effects of quercetin on specific activity of carboxylesterase and glutathione S-transferases in Bemisia tabaci[J]. Chinese Bulletin of Entomology, 2006, 43(4): 491-495.(in Chinese with English abstract)
[48]	董钧锋, 张继红, 王琛柱. 植物次生物质对烟青虫和棉铃虫食物利用及中肠解毒酶活性的影响[J]. 昆虫学报, 2002, 45(3): 296-300.
	DONG J F, ZHANG J H, WANG C Z. Effects of plant allelochemicals on nutritional utilization and detoxication enzyme activities in two Helicoverpa species[J]. Acta Entomologica Sinica, 2002, 45(3): 296-300.(in Chinese with English abstract)

外源钙浸种对小麦防御酶和麦二叉蚜体内解毒酶活性的影响

Effect of seed soaking with exogenous calcium on activities of defense enzymes in wheat and detoxifying enzymes in Schizaphis graminum (Rondani)

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