优质小麦品种温麦10号籽粒蛋白质组学分析

doi:10.3969/j.issn.1004-1524.20240036

浙江农业学报 ›› 2024, Vol. 36 ›› Issue (11): 2437-2446.DOI: 10.3969/j.issn.1004-1524.20240036

优质小麦品种温麦10号籽粒蛋白质组学分析

刘永安¹^,²(), 黄业昌¹^,², 岳高红¹^,², 高锡腾¹^,², 邓立章¹^,², 潘彬荣¹^,²^,^*()

1.浙南作物育种重点实验室, 浙江温州 325006
2.温州市农业科学研究院,浙江温州 325006

收稿日期:2024-01-05 出版日期:2024-11-25 发布日期:2024-11-27
作者简介:刘永安(1980—),男,河南太康人,博士,副教授,主要从事小麦、鲜食玉米遗传育种研究。E-mail: liuanliuan123@163.com
通讯作者: *潘彬荣,E-mail: wzpbrong@163.com
基金资助:
“十四五”浙江省农业(旱粮新品种选育)新品种选育重大科技专项(2021C02064-3);温州市农业新品种选育协作组项目(2019ZX006-5)

Proteomic analysis of grain of high-quality wheat variety Wenmai 10

LIU Yongan¹^,²(), HUANG Yechang¹^,², YUE Gaohong¹^,², GAO Xiteng¹^,², DENG Lizhang¹^,², PAN Binrong¹^,²^,^*()

1. South Zhejiang Key Laboratory of Crop Breeding, Wenzhou 325006, Zhejiang, China
2. Wenzhou Academy of Agricultural Sciences, Wenzhou 325006, Zhejiang, China

Received:2024-01-05 Online:2024-11-25 Published:2024-11-27

摘要/Abstract

摘要：

为探究温麦10号成为优质素面小麦品种的原因,以温麦10号和扬麦20(对照)为材料,采用非标记定量蛋白质组学(Label-free)技术结合生物信息学分析法,对差异表达蛋白质进行鉴定并对其进行功能分析。结果表明,两个小麦品种中共鉴定出78个显著性差异表达蛋白质,其中温麦10号有40个蛋白质上调,38个蛋白质下调。同时,还鉴定出207个有无差异表达蛋白质,其中107个蛋白质只在温麦10号籽粒中表达,100个蛋白质只在扬麦20籽粒中表达。对差异表达蛋白质进行功能分析,表明高(低)分子量谷蛋白亚基、醇溶蛋白及类燕麦贮藏蛋白可能是影响温麦10号和扬麦20品质的重要因素。

关键词: 温麦10号, 小麦, 蛋白质组, 素面

Abstract:

In order to explore the reasons why Wenmai 10 became a high-quality wheat variety of hand-made dry noddles, Wenmai 10 and Yangmai 20 (control) were used as materials and Label-free quantitative proteomics (label-free) technology combined with bioinformatics analysis was used to identify and perform functional analysis of different-expressed proteins. The results showed that a total of 78 differentially expressed proteins were identified between the two wheat varieties, of which 40 proteins were up-regulated and 38 proteins were down-regulated in Wenmai 10. At the same time, 207 proteins presence/absence differential expression were also identified, of which 107 proteins were only expressed in Wenmai 10 and 100 proteins were only expressed in Yangmai 20. The functional analysis of different-expressed proteins showed that high (low) molecular weight glutenin subunits, gliadin and avenin-like proteins might be important factors affecting the quality of Wenmai 10 and Yangmai 20.

Key words: Wenmai 10, wheat, proteome, hand-made dry noddles

中图分类号:

S512.1

刘永安, 黄业昌, 岳高红, 高锡腾, 邓立章, 潘彬荣. 优质小麦品种温麦10号籽粒蛋白质组学分析[J]. 浙江农业学报, 2024, 36(11): 2437-2446.

LIU Yongan, HUANG Yechang, YUE Gaohong, GAO Xiteng, DENG Lizhang, PAN Binrong. Proteomic analysis of grain of high-quality wheat variety Wenmai 10[J]. Acta Agriculturae Zhejiangensis, 2024, 36(11): 2437-2446.

图/表 7

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Uniprot登录号 Uniprot ID	蛋白名称 Protein name	LFQ表达量强度值 LFQ intensity		表达倍数 Fold change	温麦10号/扬麦20 Wenmai 10/ Yangmai 20
Uniprot登录号 Uniprot ID	蛋白名称 Protein name	温麦10号 Wenmai 10	扬麦20 Yangmai 20	表达倍数 Fold change	温麦10号/扬麦20 Wenmai 10/ Yangmai 20
A0A060MZP1	高分子量谷蛋白亚基(片段) High molecular weight glutenin subunit (Fragment)	17 786 666 667	3 153 533 333	5.64	上调 Up-regulated
A0A060N0C7	低分子量谷蛋白亚基(片段) Low molecular weight glutenin subunit (Fragment)	0	1 102 735 000	—	无/有 Absence/presence
B6UKM6	γ-醇溶蛋白 Gamma-gliadin	1 030 310 000	243 953 667	4.22	上调 Up-regulated
A0A0E3Z5U5	α-醇溶蛋白(片段) Alpha-gliadin (Fragment)	594 563 333	1 249 233 333	0.48	下调 Down-regulated
Q8W3W6	低分子量谷蛋白亚基3型Ⅱ (片段) Low-molecular-weight glutenin subunit group 3	9 279 333 333	0	—	有/无 Presence/absence
	type Ⅱ (Fragment)
A0A173DQZ7	b型avenin-like蛋白 Type-b avenin-like protein	467 986 667	0	—	有/无 Presence/absence
A0A173DQZ4	b型avenin-like蛋白 Type-b avenin-like protein	3 833 066 667	0	—	有/无 Presence/absence
A0A290XYZ9	γ-醇溶蛋白(片段) Gamma-gliadin (Fragment)	28 207 000	0	—	有/无 Presence/absence
A0A2U8T924	高分子量谷蛋白亚基 High molecular weight glutenin subunit	26 378 333	976 016 667	0.03	下调 Down-regulated
A0A3B6ILV9	未知蛋白 Uncharacterized protein	1 852 276 667	7 512 766 667	0.25	下调 Down-regulated
A0A3B6LUV8	未知蛋白 Uncharacterized protein	84 140 267	6 697 966 667	0.01	下调 Down-regulated
A0A3B6R7B8	AAI结构域蛋白 AAI domain-containing protein	67 002 333	258 986 667	0.26	下调 Down-regulated
B2BZD0	低分子量谷蛋白亚基0359D24-S LMW-s glutenin subunit 0359D24-S	0	589 020 000	—	无/有 Absence/presence
Q5MFQ0	低分子量谷蛋白亚基 Low molecular weight glutenin	262 726 667	2 895 566 667	0.09	下调 Down-regulated
B7U6L5	球蛋白3B Globulin 3B	27 124 500	0	—	有/无 Presence/absence
D2KFH0	麦醇溶蛋白/avenin-like种子蛋白 Gliadin/avenin-like seed protein	120 316 667	580 800 000	0.21	下调 Down-regulated
D3U326	低分子量谷蛋白亚基D3-1 Low molecular weight glutenin subunit D3-1	2 432 333 333	912 520 000	2.67	上调 Up-regulated
F8SGN5	低分子量谷蛋白亚基 Low-molecular-weight glutenin subunit	0	121 140 500	—	无/有 Absence/presence
F8SGN9	低分子量谷蛋白亚基 Low-molecular-weight glutenin subunit	1 114 736 667	179 730 000	14.49	上调 Up-regulated
Q0Q5D8	高分子量谷蛋白亚基 High-molecular-weight glutenin	2 027 833 333	0	—	有/无 Presence/absence
Q8W3W8	低分子量谷蛋白亚基3型Ⅱ (片段) Low-molecular-weight glutenin subunit group 3	1 031 740 000	0	—	有/无 Presence/absence
	type Ⅱ (Fragment)

Uniprot登录号 Uniprot ID	蛋白名称 Protein name	LFQ表达量强度值 LFQ intensity		表达倍数 Fold change	温麦10号/扬麦20 Wenmai 10/ Yangmai 20
Uniprot登录号 Uniprot ID	蛋白名称 Protein name	温麦10号 Wenmai 10	扬麦20 Yangmai 20	表达倍数 Fold change	温麦10号/扬麦20 Wenmai 10/ Yangmai 20
A0A060MZP1	高分子量谷蛋白亚基(片段) High molecular weight glutenin subunit (Fragment)	17 786 666 667	3 153 533 333	5.64	上调 Up-regulated
A0A060N0C7	低分子量谷蛋白亚基(片段) Low molecular weight glutenin subunit (Fragment)	0	1 102 735 000	—	无/有 Absence/presence
B6UKM6	γ-醇溶蛋白 Gamma-gliadin	1 030 310 000	243 953 667	4.22	上调 Up-regulated
A0A0E3Z5U5	α-醇溶蛋白(片段) Alpha-gliadin (Fragment)	594 563 333	1 249 233 333	0.48	下调 Down-regulated
Q8W3W6	低分子量谷蛋白亚基3型Ⅱ (片段) Low-molecular-weight glutenin subunit group 3	9 279 333 333	0	—	有/无 Presence/absence
	type Ⅱ (Fragment)
A0A173DQZ7	b型avenin-like蛋白 Type-b avenin-like protein	467 986 667	0	—	有/无 Presence/absence
A0A173DQZ4	b型avenin-like蛋白 Type-b avenin-like protein	3 833 066 667	0	—	有/无 Presence/absence
A0A290XYZ9	γ-醇溶蛋白(片段) Gamma-gliadin (Fragment)	28 207 000	0	—	有/无 Presence/absence
A0A2U8T924	高分子量谷蛋白亚基 High molecular weight glutenin subunit	26 378 333	976 016 667	0.03	下调 Down-regulated
A0A3B6ILV9	未知蛋白 Uncharacterized protein	1 852 276 667	7 512 766 667	0.25	下调 Down-regulated
A0A3B6LUV8	未知蛋白 Uncharacterized protein	84 140 267	6 697 966 667	0.01	下调 Down-regulated
A0A3B6R7B8	AAI结构域蛋白 AAI domain-containing protein	67 002 333	258 986 667	0.26	下调 Down-regulated
B2BZD0	低分子量谷蛋白亚基0359D24-S LMW-s glutenin subunit 0359D24-S	0	589 020 000	—	无/有 Absence/presence
Q5MFQ0	低分子量谷蛋白亚基 Low molecular weight glutenin	262 726 667	2 895 566 667	0.09	下调 Down-regulated
B7U6L5	球蛋白3B Globulin 3B	27 124 500	0	—	有/无 Presence/absence
D2KFH0	麦醇溶蛋白/avenin-like种子蛋白 Gliadin/avenin-like seed protein	120 316 667	580 800 000	0.21	下调 Down-regulated
D3U326	低分子量谷蛋白亚基D3-1 Low molecular weight glutenin subunit D3-1	2 432 333 333	912 520 000	2.67	上调 Up-regulated
F8SGN5	低分子量谷蛋白亚基 Low-molecular-weight glutenin subunit	0	121 140 500	—	无/有 Absence/presence
F8SGN9	低分子量谷蛋白亚基 Low-molecular-weight glutenin subunit	1 114 736 667	179 730 000	14.49	上调 Up-regulated
Q0Q5D8	高分子量谷蛋白亚基 High-molecular-weight glutenin	2 027 833 333	0	—	有/无 Presence/absence
Q8W3W8	低分子量谷蛋白亚基3型Ⅱ (片段) Low-molecular-weight glutenin subunit group 3	1 031 740 000	0	—	有/无 Presence/absence
	type Ⅱ (Fragment)

Uniprot登录号 Uniprot ID	蛋白名称 Protein name	亚细胞定位 Subcellular localization	结构域 Domain
A0A060MZP1	高分子量谷蛋白亚基(片段) High molecular weight glutenin subunit (Fragment)	细胞核 Nuclear	高分子量谷蛋白亚基 High molecular weight glutenin subunit
A0A060N0C7	低分子量谷蛋白亚基(片段) Low molecular weight glutenin subunit (Fragment)	细胞核 Nuclear	富半胱氨酸麦醇溶蛋白N端 Cys-rich Gliadin N-terminal
B6UKM6	γ-醇溶蛋白 Gamma-gliadin	细胞外;细胞核 Extracellular; Nuclear	富半胱氨酸麦醇溶蛋白N端 Cys-rich Gliadin N-terminal
A0A0E3Z5U5	α-醇溶蛋白(片段) Alpha-gliadin (Fragment)	细胞核 Nuclear	蛋白酶抑制剂/种子储存/LTP家族 Protease inhibitor/seed storage/LTP family
Q8W3W6	低分子量谷蛋白亚基3型Ⅱ (片段) Low-molecular-weight glutenin subunit group 3 type Ⅱ (Fragment)	细胞核 Nuclear	富半胱氨酸麦醇溶蛋白N端 Cys-rich Gliadin N-terminal
A0A173DQZ7	b型avenin-like蛋白 Type-b avenin-like protein	细胞外;细胞核 Extracellular; Nuclear	富半胱氨酸麦醇溶蛋白N端 Cys-rich Gliadin N-terminal
A0A173DQZ4	b型avenin-like蛋白 Type-b avenin-like protein	细胞外;细胞核 Extracellular; Nuclear	富半胱氨酸麦醇溶蛋白N端 Cys-rich Gliadin N-terminal
A0A290XYZ9	γ-醇溶蛋白(片段) Gamma-gliadin (Fragment)	细胞外;细胞核 Extracellular; Nuclear	富半胱氨酸麦醇溶蛋白N端 Cys-rich Gliadin N-terminal
A0A2U8T924	高分子量谷蛋白亚基 High molecular weight glutenin subunit	细胞核 Nuclear	高分子量谷蛋白亚基 High molecular weight glutenin subunit
A0A3B6ILV9	未知蛋白Uncharacterized protein	细胞核Nuclear	Cupin
A0A3B6LUV8	未知蛋白Uncharacterized protein	细胞核Nuclear	Cupin
A0A3B6R7B8	AAI结构域蛋白 AAI domain-containing protein	细胞外 Extracellular	富半胱氨酸麦醇溶蛋白N端 Cys-rich Gliadin N-terminal
B2BZD0	低分子量谷蛋白亚基0359D24-S LMW-s glutenin subunit 0359D24-S	细胞外;细胞核 Extracellular; Nuclear	富半胱氨酸麦醇溶蛋白N端 Cys-rich Gliadin N-terminal
Q5MFQ0	低分子量谷蛋白亚基 Low molecular weight glutenin	细胞核 Nuclear	富半胱氨酸麦醇溶蛋白N端 Cys-rich Gliadin N-terminal
B7U6L5	球蛋白3B Globulin 3B	细胞核Nuclear	Cupin
D2KFH0	麦醇溶蛋白/avenin-like种子蛋白 Gliadin/avenin-like seed protein	细胞外 Extracellular	富半胱氨酸麦醇溶蛋白N端 Cys-rich Gliadin N-terminal
D3U326	低分子量谷蛋白亚基D3-1 Low molecular weight glutenin subunit D3-1	细胞核 Nuclear	富半胱氨酸麦醇溶蛋白N端 Cys-rich Gliadin N-terminal
F8SGN5	低分子量谷蛋白亚基 Low-molecular-weight glutenin subunit	细胞外;细胞核 Extracellular; Nuclear	富半胱氨酸麦醇溶蛋白N端 Cys-rich Gliadin N-terminal
F8SGN9	低分子量谷蛋白亚基 Low-molecular-weight glutenin subunit	细胞外;细胞核 Extracellular; Nuclear	富半胱氨酸麦醇溶蛋白N端 Cys-rich Gliadin N-terminal
Q0Q5D8	高分子量谷蛋白亚基 High-molecular-weight glutenin	细胞核 Nuclear	高分子量谷蛋白亚基 High molecular weight glutenin subunit
Q8W3W8	低分子量谷蛋白亚基3型Ⅱ (片段) Low-molecular-weight glutenin subunit group 3 type Ⅱ (Fragment)	细胞外;细胞核 Extracellular; Nuclear	富半胱氨酸麦醇溶蛋白N端 Cys-rich Gliadin N-terminal

Uniprot登录号 Uniprot ID	蛋白名称 Protein name	亚细胞定位 Subcellular localization	结构域 Domain
A0A060MZP1	高分子量谷蛋白亚基(片段) High molecular weight glutenin subunit (Fragment)	细胞核 Nuclear	高分子量谷蛋白亚基 High molecular weight glutenin subunit
A0A060N0C7	低分子量谷蛋白亚基(片段) Low molecular weight glutenin subunit (Fragment)	细胞核 Nuclear	富半胱氨酸麦醇溶蛋白N端 Cys-rich Gliadin N-terminal
B6UKM6	γ-醇溶蛋白 Gamma-gliadin	细胞外;细胞核 Extracellular; Nuclear	富半胱氨酸麦醇溶蛋白N端 Cys-rich Gliadin N-terminal
A0A0E3Z5U5	α-醇溶蛋白(片段) Alpha-gliadin (Fragment)	细胞核 Nuclear	蛋白酶抑制剂/种子储存/LTP家族 Protease inhibitor/seed storage/LTP family
Q8W3W6	低分子量谷蛋白亚基3型Ⅱ (片段) Low-molecular-weight glutenin subunit group 3 type Ⅱ (Fragment)	细胞核 Nuclear	富半胱氨酸麦醇溶蛋白N端 Cys-rich Gliadin N-terminal
A0A173DQZ7	b型avenin-like蛋白 Type-b avenin-like protein	细胞外;细胞核 Extracellular; Nuclear	富半胱氨酸麦醇溶蛋白N端 Cys-rich Gliadin N-terminal
A0A173DQZ4	b型avenin-like蛋白 Type-b avenin-like protein	细胞外;细胞核 Extracellular; Nuclear	富半胱氨酸麦醇溶蛋白N端 Cys-rich Gliadin N-terminal
A0A290XYZ9	γ-醇溶蛋白(片段) Gamma-gliadin (Fragment)	细胞外;细胞核 Extracellular; Nuclear	富半胱氨酸麦醇溶蛋白N端 Cys-rich Gliadin N-terminal
A0A2U8T924	高分子量谷蛋白亚基 High molecular weight glutenin subunit	细胞核 Nuclear	高分子量谷蛋白亚基 High molecular weight glutenin subunit
A0A3B6ILV9	未知蛋白Uncharacterized protein	细胞核Nuclear	Cupin
A0A3B6LUV8	未知蛋白Uncharacterized protein	细胞核Nuclear	Cupin
A0A3B6R7B8	AAI结构域蛋白 AAI domain-containing protein	细胞外 Extracellular	富半胱氨酸麦醇溶蛋白N端 Cys-rich Gliadin N-terminal
B2BZD0	低分子量谷蛋白亚基0359D24-S LMW-s glutenin subunit 0359D24-S	细胞外;细胞核 Extracellular; Nuclear	富半胱氨酸麦醇溶蛋白N端 Cys-rich Gliadin N-terminal
Q5MFQ0	低分子量谷蛋白亚基 Low molecular weight glutenin	细胞核 Nuclear	富半胱氨酸麦醇溶蛋白N端 Cys-rich Gliadin N-terminal
B7U6L5	球蛋白3B Globulin 3B	细胞核Nuclear	Cupin
D2KFH0	麦醇溶蛋白/avenin-like种子蛋白 Gliadin/avenin-like seed protein	细胞外 Extracellular	富半胱氨酸麦醇溶蛋白N端 Cys-rich Gliadin N-terminal
D3U326	低分子量谷蛋白亚基D3-1 Low molecular weight glutenin subunit D3-1	细胞核 Nuclear	富半胱氨酸麦醇溶蛋白N端 Cys-rich Gliadin N-terminal
F8SGN5	低分子量谷蛋白亚基 Low-molecular-weight glutenin subunit	细胞外;细胞核 Extracellular; Nuclear	富半胱氨酸麦醇溶蛋白N端 Cys-rich Gliadin N-terminal
F8SGN9	低分子量谷蛋白亚基 Low-molecular-weight glutenin subunit	细胞外;细胞核 Extracellular; Nuclear	富半胱氨酸麦醇溶蛋白N端 Cys-rich Gliadin N-terminal
Q0Q5D8	高分子量谷蛋白亚基 High-molecular-weight glutenin	细胞核 Nuclear	高分子量谷蛋白亚基 High molecular weight glutenin subunit
Q8W3W8	低分子量谷蛋白亚基3型Ⅱ (片段) Low-molecular-weight glutenin subunit group 3 type Ⅱ (Fragment)	细胞外;细胞核 Extracellular; Nuclear	富半胱氨酸麦醇溶蛋白N端 Cys-rich Gliadin N-terminal

优质小麦品种温麦10号籽粒蛋白质组学分析

Proteomic analysis of grain of high-quality wheat variety Wenmai 10

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