图1 SmMYB13基因全长PCR产物

Fig.1 The full-length PCR product of the SmMYB13 gene

图2 SmMYB13蛋白亲水性预测(A)和三级结构预测(B、C)

Fig.2 Prediction of hydrophilicity (A) and tertiary structure (B and C) of SmMYB13 protein

图3 不同物种MYB13的系统进化树

Fig.3 Phylogenetic tree of MYB13 in different species

图4 SmMYB13基因转录激活活性的验证(A)及其在茄子不同器官中的相对表达水平(B) 柱上无相同小写字母表示差异显著(p<0.05)。

Fig.4 Verification of the transcriptional activation activity of the SmMYB13 gene (A) and its relative expression levels in different organs of Solanum melongena L. (B) The bars marked without the same lowercase letter indicated significant differences (p<0.05).

图5 不同非生物胁迫下茄子幼苗叶片中SmMYB13基因的相对表达水平 “****”表示与野生型相比在p<0.001水平存在显著差异,“ns”表示与野生型相比差异不显著(p>0.05)。同一处理不同时间无相同小写字母表示差异显著(p<0.05)。下同。

Fig.5 Relative expression levels of the SmMYB13 gene in eggplant seedling leaves under different abiotic stresses “****” indicates significant differences compared with the wild type at the p<0.001 level,“ns” indicates no significant difference compared with the wild type (p>0.05). Different lowercase letters for the same treatment at different sampling times indicate significant differences (p<0.05). The same as below.

图6 SmMYB13基因过表达拟南芥的Basta抗性筛选(A)、PCR(B)和qRT-PCR(C)鉴定 +表示阳性对照(质粒);-表示阴性对照(野生型植株);数字1~10表示10个转基因阳性株系。Col-0表示野生型植株,OE表示SmMYB13基因过表达株系。

Fig.6 Identification of SmMYB13 overexpression Arabidopsis thaliana lines by Basta resistance screening (A), PCR (B), and qRT-PCR (C) “+” indicates the positive control (plasmid); “-” indicates the negative control (wild-type plant); Numbers 1-10 represent ten transgenic positive lines. Col-0 indicates the wild-type plant, and OE represents the SmMYB13 overexpression line.

图7 干旱胁迫下拟南芥叶片的失水率、TB染色结果和植株的表型 A,干旱处理后0~6 h拟南芥叶片的失水率,“**”“****”分别表示与野生型相比在p<0.01和p<0.001水平存在显著差异;B,干旱处理后拟南芥叶片的TB染色结果;C,干旱处理10 d后拟南芥植株的表型。Col-0表示野生型植株,OE表示SmMYB13基因过表达株系。下同。

Fig.7 Water loss rate and Trypan blue (TB) staining results of Arabidopsis thaliana leaves, and phenotypes of Arabidopsis thaliana plants under drought stress A, Water loss rate of Arabidopsis thaliana leaves during 0-6 h under drought treatment, “**” and “****” indicate significant differences compared with the wild type at p <0.01 and p<0.001 levels, respectively; B, Trypan blue (TB) staining results of Arabidopsis thaliana leaves after drought treatment; C, Phenotype of Arabidopsis thaliana plants after 10 days of drought treatment. Col-0 indicates the wild-type plant, and OE represents the SmMYB13 overexpression lines. The same as below.

图8 干旱胁迫后拟南芥叶片的POD(A)、CAT(B)、SOD(C)活性和MDA含量(D) POD,过氧化物酶;CAT,过氧化氢酶;SOD,超氧化物歧化酶;MDA,丙二醛。

Fig.8 Activities of POD (A), CAT (B), SOD (C) and MDA content (D) in Arabidopsis thaliana leaves after drought stress POD, Peroxidase; CAT, Catalase; SOD, Superoxide dismutase; MDA, Malondialdehyde.

图9 不同培养基处理7 d后拟南芥根部的表型(A~C)和主根长度(D) 相同培养基中无相同小写字母表示在p<0.05水平存在显著差异。

Fig.9 Phenotype (A-C) and primary root length (D) of Arabidopsis thaliana roots after 7-day treatment with different culture media The bars marked without the same lowercase letter in the same culture medium indicate significant differences (p<0.05).

图10 ABA处理下拟南芥中ABA应答基因和调节基因的相对表达水平 A~F,ABA处理0~12 h时ABA应答基因的相对表达水平;G,ABA处理3 h时调节基因的相对表达水平。“*”“**”分别表示与野生型相比在p<0.05、p<0.01水平存在显著差异。

Fig.10 The relative expression levels of ABA-responsive genes and regulatory genes in Arabidopsis thaliana under ABA treatment A-F, Relative expression levels of ABA-responsive genes within 0-12 h of ABA treatment; G, Relative expression levels of regulatory genes after 3 h of ABA treatment. “*” and “**” indicate significant differences compared with wild type at p<0.05 and p<0.01 levels, respectively.