Effects of purified rice bran on antioxidant capacity and gut microbiota in D-galactose-induced subacute aging mice

doi:10.3969/j.issn.1004-1524.20250344

Acta Agriculturae Zhejiangensis ›› 2026, Vol. 38 ›› Issue (5): 845-856.DOI: 10.3969/j.issn.1004-1524.20250344

• Animal Science • Previous Articles Next Articles

Effects of purified rice bran on antioxidant capacity and gut microbiota in D-galactose-induced subacute aging mice

QIN Haisang¹^,²(), LI Jianqiang², LIU Zhaojun², WU Yuhang², GU Jie³, ZHANG Ge³, JIANG Yuanrong³, LI Jinjun², WANG Xin², KUANG Jian², BIAN Xiangyu², SHI Fangshu², CHEN Yin¹, WEN Zhengshun¹^,⁴^,^*(), LI Xiaoqiong²^,^*()

¹ Food and Pharmacy College, Zhejiang Ocean University, Zhoushan 316022, Zhejiang, China
² State Key Laboratory for Quality and Safety of Agro-Products, Institute of Food Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
³ Wilmar (Shanghai) Biotechnology Research & Development Center Co., Ltd., Shanghai 200137, China
⁴ Xianghu Laboratory, Hangzhou 311231, China

Received:2025-04-28 Online:2026-05-25 Published:2026-06-02

Abstract

Abstract:

Rice bran, a by-product of grain processing, is rich in dietary fiber and possesses valuable nutritional and functional properties. This study aimed to evaluate the physiological effects of purified rice bran (PRB), produced via α-amylase hydrolysis, on behavioral function, antioxidant capacity, and gut microbiota composition in a murine model of subacute aging induced by D-galactose (D-gal). The results showed that D-gal treatment significantly (p<0.05) impaired locomotor and cognitive functions. In contrast, PRB supplementation effectively alleviated these deficits dose-dependently, with the high-dose group showing the most pronounced improvement. In terms of antioxidant capacity, PRB significantly increased the superoxide dismutase (SOD) activity and glutathione (GSH) content, yet reduced malondialdehyde (MDA) content, thereby alleviating oxidative stress. Gut microbiota analysis revealed that PRB regulated microbial diversity, suppressed harmful bacteria such as Desulfovibrio, and promoted the growth of beneficial genera such as Bifidobacterium, Rikenella, promoting microbial homeostasis. Moreover, PRB significantly enhanced the production of short-chain fatty acid (SCFA), including acetic, propionic, butyric, and valeric acids, contributing to intestinal pH value regulation and homeostasis. Overall, PRB exerted synergistic effects on oxidative stress and gut dysbiosis in aging mice, suggesting its potential to promote gut health and overall well-being.

Key words: purified rice bran, aging, gut microbiota, short-chain fatty acid, antioxidation

CLC Number:

TS201.4

QIN Haisang, LI Jianqiang, LIU Zhaojun, WU Yuhang, GU Jie, ZHANG Ge, JIANG Yuanrong, LI Jinjun, WANG Xin, KUANG Jian, BIAN Xiangyu, SHI Fangshu, CHEN Yin, WEN Zhengshun, LI Xiaoqiong. Effects of purified rice bran on antioxidant capacity and gut microbiota in D-galactose-induced subacute aging mice[J]. Acta Agriculturae Zhejiangensis, 2026, 38(5): 845-856.

Figures/Tables 8

Table 1 Comparison of rice bran traits before and after purification

样品 Sample	c_sta/%	c_pro/%	c_fat/%	c_ash/%	c_TDF/%	c_WEA/%	c_ara/%	c_FTP/ (mg·g^-1)	c_BTP/ (mg·g^-1)
RB	19.35±0.31	19.42±0.34	2.47±0.09	12.56±0.14	38.00±0.56	0.20±0.02	16.49±0.12	2.05±0.04	6.51±0.20
PRB	2.12±0.33	26.48±0.55	2.00±0.36	8.72±0.17	64.01±0.27	0.24±0.05	20.18±0.39	2.69±0.06	6.53±0.07

Table 2 Diet composition of mice in each group Unit: g

原料 Ingredient	各组每1 kg饲料的原料构成Ingredient composition of feed per kilogram for each group
原料 Ingredient	CON	MOD	PRBL	PRBM	PRBH
酪蛋白Casein	123.8	123.8	123.8	123.8	123.8
L-胱氨酸L-Cystine	1.8	1.8	1.8	1.8	1.8
玉米淀粉Corn starch	383.2	383.2	323.7	264.2	204.7
麦芽糊精10 Maltodextrin 10	125	125	125	125	125
蔗糖Sucrose	100	100	100	100	100
纯化米糠Purified rice bran	0	0	100	200	300
纤维素BW200 Cellulose BW200	121.5	121.5	81	40.5	0
大豆油Soybean oil	40	40	40	40	40
猪油Lard	57.2	57.2	57.2	57.2	57.2
混合矿物质 S10022M Mineral mix S10022M	35	35	35	35	35
混合维生素 V10037 Vitamin mix V10037	10	10	10	10	10
酒石酸氢胆碱Choline bitartrate	2.5	2.5	2.5	2.5	2.5
合计 Total	1 000	1 000	1 000	1 000	1 000

Fig.1 Effect of different treatments on behavioral performance of mice Panels A and B show the results of the open field test; Panel C shows the results of the Y-maze test; Panel D shows the results of the novel object recognition test; Panel E shows the results of the pole climbing test. Bars marked without the same letters indicate significant differences at p<0.05.

Fig.2 Effect of different treatments on oxidative stress in serum of mice T-AOC, Total antioxidant capacity; ASOD, Superoxide dismutase activity; cGSH, Glutathione content; cMDA, Malondialdehyde content.

Fig.3 Effect of different treatments on short-chain fatty acids contents in feces of mice

Fig.4 Effect of different treatments on intestinal microbial diversity of mice A, Chao1 index; B, Shannon index; C, Bray _ Curtis _ PCoA; D, Jaccard _ PCoA. PCoA, Principal coordinates analysis; PCoA1, Principal coordinate 1; PCoA2, Principal coordinate 2.

Fig.5 Effect of different treatments on intestinal flora of mice A, Relative abundance of the dominant phyla at the phylum level; B, Relative abundance of the dominant genera at the genus level; C, Ratio of the relative abundance of Firmicutes to Bacteroidota (F/B) under different treatments; D-K show the relative abundances of Desulfovibrio, Lachnospiraceae_NK4A136_group, Colidextribacter, Akkermansia, Bifidobacterium, Acetatifactor, Rikenella and Rikenellaceae_RC9_gut_group under different treatments in sequence; L, Linear discriminant analysis (LDA) scores of the biomarker microbes at the genus level under different treatments.

Fig.6 Spearman correlation analysis of the relative abundance of gut microbiota (genus level) with short-chain fatty acids contents and antioxidant indices of mice cAA, Acetic acid content; cPA, Propanoic acid content; cBA, Butyric acid content; cVA, Valeric acid content; T-AOC, Total antioxidant capacity; ASOD, Superoxide dismutase activity; cGSH, Glutathione content; cMDA, Malondialdehyde content. “*” and “**” indicate significant correlation at p<0.05 and p<0.01 level, respectively.

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Effects of purified rice bran on antioxidant capacity and gut microbiota in D-galactose-induced subacute aging mice

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